Mutagenetix > Incidental Mutation

Incidental Mutation 'R0742:Vmn2r12'

70721

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

038923-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R0742 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109233715-109245730 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 109234281 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 644 (T644S)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

possibly damaging
Transcript: ENSMUST00000095922
AA Change: T644S

PolyPhen 2 Score 0.546 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: T644S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 94.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg2	T	A	6: 58,655,311 (GRCm39)	D415E	probably benign	Het
Aff3	A	G	1: 38,666,189 (GRCm39)	W12R	probably damaging	Het
Aldh3b2	G	A	19: 4,031,034 (GRCm39)	G428S	probably damaging	Het
Arhgef10l	A	G	4: 140,264,156 (GRCm39)	L736P	probably damaging	Het
Baz2a	G	T	10: 127,949,535 (GRCm39)	E374*	probably null	Het
Casd1	T	C	6: 4,635,888 (GRCm39)		probably null	Het
Cct2	A	T	10: 116,891,151 (GRCm39)		probably null	Het
Cdc42bpg	G	A	19: 6,368,605 (GRCm39)		probably null	Het
Copg2	T	C	6: 30,840,548 (GRCm39)		probably null	Het
Fbxw15	C	T	9: 109,384,624 (GRCm39)		probably null	Het
Fyb1	A	G	15: 6,664,297 (GRCm39)	D460G	probably benign	Het
Lgr6	C	T	1: 134,921,748 (GRCm39)	A199T	probably damaging	Het
Lyz1	A	T	10: 117,125,022 (GRCm39)		probably null	Het
Mroh3	A	T	1: 136,118,718 (GRCm39)	I533N	probably damaging	Het
Otogl	A	G	10: 107,702,601 (GRCm39)	V684A	possibly damaging	Het
Pcdh15	A	G	10: 74,457,129 (GRCm39)	D1302G	probably damaging	Het
Plec	A	T	15: 76,056,983 (GRCm39)	I4183N	probably damaging	Het
Rpe	C	T	1: 66,754,300 (GRCm39)	T124I	probably benign	Het
Rufy4	T	C	1: 74,185,875 (GRCm39)	I514T	probably benign	Het
Scap	T	A	9: 110,210,327 (GRCm39)	L912Q	probably damaging	Het
Sec61a2	T	C	2: 5,881,359 (GRCm39)	D264G	probably benign	Het
Slc4a3	T	A	1: 75,532,725 (GRCm39)	I995K	probably damaging	Het
Sptbn2	T	C	19: 4,769,011 (GRCm39)	I48T	possibly damaging	Het
Steap3	C	T	1: 120,169,313 (GRCm39)	R328H	possibly damaging	Het
Thoc2l	G	A	5: 104,670,020 (GRCm39)	S1514N	probably benign	Het
Tmprss13	T	C	9: 45,243,765 (GRCm39)	F167S	probably damaging	Het
Ttc3	G	A	16: 94,260,739 (GRCm39)	C1408Y	probably benign	Het
Twf1	A	G	15: 94,483,411 (GRCm39)	M99T	probably damaging	Het
Unc80	A	G	1: 66,567,052 (GRCm39)	N886S	possibly damaging	Het
Vps13b	A	G	15: 35,794,507 (GRCm39)	S2306G	probably benign	Het
Xpo1	T	C	11: 23,244,682 (GRCm39)	V1020A	possibly damaging	Het
Ylpm1	T	A	12: 85,075,886 (GRCm39)	N870K	probably benign	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109,245,541 (GRCm39)	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109,234,125 (GRCm39)	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109,239,716 (GRCm39)	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109,240,893 (GRCm39)	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109,240,025 (GRCm39)	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109,234,343 (GRCm39)	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109,233,858 (GRCm39)	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109,238,351 (GRCm39)	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109,239,936 (GRCm39)	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109,240,765 (GRCm39)	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109,239,755 (GRCm39)	nonsense	probably null
R0529:Vmn2r12	UTSW	5	109,240,714 (GRCm39)	missense	probably benign
R0715:Vmn2r12	UTSW	5	109,238,373 (GRCm39)	missense	probably benign	0.10
R0894:Vmn2r12	UTSW	5	109,235,716 (GRCm39)	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109,239,763 (GRCm39)	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R1388:Vmn2r12	UTSW	5	109,240,840 (GRCm39)	missense	possibly damaging	0.56
R1549:Vmn2r12	UTSW	5	109,240,696 (GRCm39)	missense	probably benign	0.06
R1766:Vmn2r12	UTSW	5	109,239,910 (GRCm39)	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109,239,594 (GRCm39)	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109,239,942 (GRCm39)	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109,239,340 (GRCm39)	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109,238,370 (GRCm39)	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109,239,412 (GRCm39)	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109,234,301 (GRCm39)	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109,234,379 (GRCm39)	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109,240,852 (GRCm39)	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109,239,544 (GRCm39)	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109,239,372 (GRCm39)	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109,238,261 (GRCm39)	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109,239,684 (GRCm39)	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109,234,483 (GRCm39)	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109,240,666 (GRCm39)	missense	probably benign	0.06
R5753:Vmn2r12	UTSW	5	109,239,670 (GRCm39)	missense	probably damaging	1.00
R5797:Vmn2r12	UTSW	5	109,233,736 (GRCm39)	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109,240,763 (GRCm39)	missense	probably benign
R6162:Vmn2r12	UTSW	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109,233,866 (GRCm39)	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109,240,771 (GRCm39)	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109,245,655 (GRCm39)	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109,239,811 (GRCm39)	missense	possibly damaging	0.95
R7341:Vmn2r12	UTSW	5	109,234,113 (GRCm39)	missense	possibly damaging	0.74
R7383:Vmn2r12	UTSW	5	109,240,684 (GRCm39)	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109,239,615 (GRCm39)	missense	probably damaging	1.00
R7749:Vmn2r12	UTSW	5	109,233,920 (GRCm39)	missense	probably damaging	0.99
R7861:Vmn2r12	UTSW	5	109,235,829 (GRCm39)	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109,234,307 (GRCm39)	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109,239,747 (GRCm39)	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109,238,349 (GRCm39)	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109,234,074 (GRCm39)	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109,239,952 (GRCm39)	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109,234,522 (GRCm39)	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109,234,033 (GRCm39)	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109,233,885 (GRCm39)	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109,240,910 (GRCm39)	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109,234,203 (GRCm39)	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R9375:Vmn2r12	UTSW	5	109,233,986 (GRCm39)	missense	probably damaging	1.00
R9524:Vmn2r12	UTSW	5	109,239,823 (GRCm39)	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109,239,322 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109,240,646 (GRCm39)	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109,239,303 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGACCAAGTTAGGTGCCCCTGATG -3'
(R):5'- TGCCAGCAGATATGGAACAGTGTG -3'

Sequencing Primer
(F):5'- CCCTGATGCCAGCATCC -3'
(R):5'- ACTGCCTTCAAAGAGCTGTG -3'

Posted On

2013-09-30