Mutagenetix > Incidental Mutation

Incidental Mutation 'R9341:Tas2r144'

707487

Institutional Source

Beutler Lab

Gene Symbol

Tas2r144

Ensembl Gene

ENSMUSG00000051917

Gene Name

taste receptor, type 2, member 144

Synonyms

mt2r33, Tas2r44

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R9341 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

42192262-42193221 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 42193066 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 269 (I269F)

Ref Sequence

ENSEMBL: ENSMUSP00000067734 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063489]

AlphaFold

Q7TQB8

Predicted Effect

probably benign
Transcript: ENSMUST00000063489
AA Change: I269F

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000067734
Gene: ENSMUSG00000051917
AA Change: I269F

Domain	Start	End	E-Value	Type
Pfam:TAS2R	12	314	9.6e-63	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acat2	T	C	17: 13,167,538 (GRCm39)	N166S	probably damaging	Het
Agap2	G	C	10: 126,927,559 (GRCm39)	G1147R	unknown	Het
Atp8b2	A	T	3: 89,855,811 (GRCm39)	F446I	possibly damaging	Het
Cacna2d3	A	T	14: 28,704,315 (GRCm39)	M822K	possibly damaging	Het
Caskin1	A	G	17: 24,723,447 (GRCm39)	E745G	probably damaging	Het
Clec4a2	G	T	6: 123,104,955 (GRCm39)	A82S	probably benign	Het
Col5a3	T	C	9: 20,704,909 (GRCm39)	N749S	unknown	Het
Col9a2	T	A	4: 120,911,483 (GRCm39)	M608K	probably benign	Het
Cope	T	C	8: 70,761,228 (GRCm39)		probably null	Het
Dennd2d	T	A	3: 106,397,730 (GRCm39)		probably null	Het
Dpys	T	C	15: 39,656,748 (GRCm39)	T440A	possibly damaging	Het
Fam186b	G	A	15: 99,177,616 (GRCm39)	A570V	probably damaging	Het
Fbln5	G	A	12: 101,737,551 (GRCm39)	T152I	probably damaging	Het
Gm16503	A	T	4: 147,625,508 (GRCm39)	M1L	unknown	Het
Gm9195	A	G	14: 72,717,500 (GRCm39)	S278P	probably damaging	Het
Hmcn2	A	G	2: 31,279,359 (GRCm39)	N1787S	probably benign	Het
Klhl33	A	G	14: 51,133,903 (GRCm39)		probably null	Het
Lrrk2	A	G	15: 91,584,618 (GRCm39)	D345G	probably benign	Het
Man2b2	A	G	5: 36,975,951 (GRCm39)	L368P	probably damaging	Het
Med23	T	A	10: 24,788,705 (GRCm39)	S1371T	probably benign	Het
Mllt3	T	A	4: 87,792,168 (GRCm39)	E109D	possibly damaging	Het
Myom2	G	A	8: 15,134,633 (GRCm39)	D479N	probably damaging	Het
Or52r1c	A	T	7: 102,735,324 (GRCm39)	R195*	probably null	Het
Pcdhga10	G	T	18: 37,880,532 (GRCm39)	A98S	probably benign	Het
Pde8a	A	T	7: 80,950,427 (GRCm39)	Q221H	probably benign	Het
Pkd1l1	T	C	11: 8,786,399 (GRCm39)	H2335R		Het
Pkd1l1	T	A	11: 8,911,305 (GRCm39)	D324V		Het
Pou2f2	G	A	7: 24,794,277 (GRCm39)	T363I	possibly damaging	Het
Prlr	A	G	15: 10,328,988 (GRCm39)	I488V	probably benign	Het
Psmd2	T	C	16: 20,475,441 (GRCm39)		probably null	Het
Rbm7	T	C	9: 48,400,969 (GRCm39)	D253G	probably damaging	Het
Ros1	A	G	10: 51,972,116 (GRCm39)		probably null	Het
S1pr3	G	T	13: 51,573,553 (GRCm39)	V245L	probably damaging	Het
Sacs	A	G	14: 61,446,219 (GRCm39)	D2755G	probably benign	Het
Scgn	T	A	13: 24,173,829 (GRCm39)		probably null	Het
Sh3rf3	A	G	10: 58,966,802 (GRCm39)	K715E	probably damaging	Het
Sox18	T	C	2: 181,312,231 (GRCm39)	E300G	probably damaging	Het
Spef2	T	A	15: 9,713,190 (GRCm39)	N394I	probably damaging	Het
Strn4	T	C	7: 16,573,827 (GRCm39)	F759L	probably damaging	Het
Tpcn1	T	C	5: 120,678,737 (GRCm39)	D606G	possibly damaging	Het
Trh	A	G	6: 92,220,823 (GRCm39)	I13T	probably benign	Het
Umodl1	T	C	17: 31,217,701 (GRCm39)	I1169T	possibly damaging	Het
Vwa3b	G	A	1: 37,153,615 (GRCm39)	D486N	probably damaging	Het
Wars2	T	C	3: 99,094,846 (GRCm39)	L47P	probably benign	Het
Zbtb14	A	G	17: 69,695,576 (GRCm39)	S425G	probably damaging	Het
Zdhhc16	T	C	19: 41,926,549 (GRCm39)	S111P	probably benign	Het

Other mutations in Tas2r144

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02349:Tas2r144	APN	6	42,193,010 (GRCm39)	missense	probably benign	0.26
IGL02644:Tas2r144	APN	6	42,192,787 (GRCm39)	missense	possibly damaging	0.89
IGL02816:Tas2r144	APN	6	42,192,539 (GRCm39)	missense	probably benign	0.03
R0375:Tas2r144	UTSW	6	42,193,058 (GRCm39)	missense	possibly damaging	0.92
R1526:Tas2r144	UTSW	6	42,192,674 (GRCm39)	missense	probably benign
R1543:Tas2r144	UTSW	6	42,192,537 (GRCm39)	missense	probably benign	0.00
R1678:Tas2r144	UTSW	6	42,192,490 (GRCm39)	missense	probably benign	0.44
R1868:Tas2r144	UTSW	6	42,192,936 (GRCm39)	missense	probably benign	0.01
R1880:Tas2r144	UTSW	6	42,193,004 (GRCm39)	missense	probably benign	0.02
R4060:Tas2r144	UTSW	6	42,192,563 (GRCm39)	missense	possibly damaging	0.82
R5173:Tas2r144	UTSW	6	42,193,048 (GRCm39)	missense	probably benign	0.40
R6108:Tas2r144	UTSW	6	42,192,691 (GRCm39)	missense	possibly damaging	0.88
R6249:Tas2r144	UTSW	6	42,192,291 (GRCm39)	nonsense	probably null
R6533:Tas2r144	UTSW	6	42,192,280 (GRCm39)	missense	probably benign
R6850:Tas2r144	UTSW	6	42,192,857 (GRCm39)	missense	possibly damaging	0.71
R7237:Tas2r144	UTSW	6	42,192,800 (GRCm39)	missense	probably damaging	0.99
R7296:Tas2r144	UTSW	6	42,192,373 (GRCm39)	missense	probably damaging	1.00
R7431:Tas2r144	UTSW	6	42,192,908 (GRCm39)	missense	probably damaging	1.00
R8206:Tas2r144	UTSW	6	42,192,325 (GRCm39)	missense	probably damaging	0.98
R9343:Tas2r144	UTSW	6	42,193,066 (GRCm39)	missense	probably benign	0.03
R9640:Tas2r144	UTSW	6	42,192,428 (GRCm39)	missense	probably benign	0.00
X0067:Tas2r144	UTSW	6	42,193,099 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGATCAATGTACTCAGCCTAGC -3'
(R):5'- AGAACTGGTTGGGTCAGGTC -3'

Sequencing Primer
(F):5'- ATGTTCATCATGGCAGCCAC -3'
(R):5'- GGGTCAGGTCTTATAATACTTCCAG -3'

Posted On

2022-04-18