Mutagenetix > Incidental Mutation

Incidental Mutation 'R9342:Klc2'

707604

Institutional Source

Beutler Lab

Gene Symbol

Klc2

Ensembl Gene

ENSMUSG00000024862

Gene Name

kinesin light chain 2

Synonyms

8030455F02Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9342 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

5107746-5118560 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 5108631 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 612 (S612P)

Ref Sequence

ENSEMBL: ENSMUSP00000112262 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025798] [ENSMUST00000025804] [ENSMUST00000113727] [ENSMUST00000113728] [ENSMUST00000116563]

AlphaFold

no structure available at present

PDB Structure

Crystal structure of the TPR domain of kinesin light chain 2 in complex with a tryptophan-acidic cargo peptide [X-RAY DIFFRACTION]

Predicted Effect

unknown
Transcript: ENSMUST00000025798
AA Change: S610P

SMART Domains

Protein: ENSMUSP00000025798
Gene: ENSMUSG00000024862
AA Change: S610P

Domain	Start	End	E-Value	Type
Pfam:Rab5-bind	69	239	4.1e-64	PFAM
Pfam:TPR_10	197	238	9.5e-9	PFAM
TPR	240	273	6.19e-1	SMART
TPR	282	315	1.16e-5	SMART
TPR	324	357	2.16e0	SMART
TPR	366	399	1.6e-3	SMART
Pfam:TPR_10	448	483	1.5e-4	PFAM
low complexity region	496	507	N/A	INTRINSIC
low complexity region	528	542	N/A	INTRINSIC
low complexity region	592	615	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000025804

SMART Domains

Protein: ENSMUSP00000025804
Gene: ENSMUSG00000024870

Domain	Start	End	E-Value	Type
RAB	9	172	4.57e-105	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000113727
AA Change: S610P

SMART Domains

Protein: ENSMUSP00000109356
Gene: ENSMUSG00000024862
AA Change: S610P

Domain	Start	End	E-Value	Type
Pfam:Rab5-bind	69	239	4.1e-64	PFAM
Pfam:TPR_10	197	238	9.5e-9	PFAM
TPR	240	273	6.19e-1	SMART
TPR	282	315	1.16e-5	SMART
TPR	324	357	2.16e0	SMART
TPR	366	399	1.6e-3	SMART
Pfam:TPR_10	448	483	1.5e-4	PFAM
low complexity region	496	507	N/A	INTRINSIC
low complexity region	528	542	N/A	INTRINSIC
low complexity region	592	615	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000113728
AA Change: S610P

SMART Domains

Protein: ENSMUSP00000109357
Gene: ENSMUSG00000024862
AA Change: S610P

Domain	Start	End	E-Value	Type
Pfam:Rab5-bind	69	239	4.1e-64	PFAM
Pfam:TPR_10	197	238	9.5e-9	PFAM
TPR	240	273	6.19e-1	SMART
TPR	282	315	1.16e-5	SMART
TPR	324	357	2.16e0	SMART
TPR	366	399	1.6e-3	SMART
Pfam:TPR_10	448	483	1.5e-4	PFAM
low complexity region	496	507	N/A	INTRINSIC
low complexity region	528	542	N/A	INTRINSIC
low complexity region	592	615	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000116563
AA Change: S612P

PolyPhen 2 Score 0.037 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000112262
Gene: ENSMUSG00000024862
AA Change: S612P

Domain	Start	End	E-Value	Type
coiled coil region	80	140	N/A	INTRINSIC
Pfam:TPR_10	197	238	3.1e-9	PFAM
TPR	240	273	6.19e-1	SMART
TPR	282	315	1.16e-5	SMART
TPR	324	357	2.16e0	SMART
TPR	366	399	1.6e-3	SMART
low complexity region	416	428	N/A	INTRINSIC
Pfam:TPR_10	450	486	1.1e-4	PFAM
low complexity region	498	509	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	594	617	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a light chain of kinesin, a molecular motor responsible for moving vesicles and organelles along microtubules. Defects in this gene are a cause of spastic paraplegia, optic atrophy, and neuropathy (SPOAN) syndrome. [provided by RefSeq, Mar 2016]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700025F22Rik	T	A	19: 11,142,411	T52S	possibly damaging	Het
A330070K13Rik	T	C	5: 130,379,035	I112V	unknown	Het
Aars2	A	G	17: 45,507,076	N75D	possibly damaging	Het
Abcf2	G	A	5: 24,573,477	R228C	probably benign	Het
Agfg2	A	G	5: 137,653,852	L415P	probably benign	Het
Alpi	A	G	1: 87,098,664	L535P	unknown	Het
Anxa9	T	G	3: 95,303,048	T69P	probably damaging	Het
Arrb2	T	A	11: 70,436,637	D79E	probably benign	Het
Bcat1	A	G	6: 145,048,606	V55A	probably benign	Het
Cacna1c	A	G	6: 119,057,374	L64P		Het
Cd247	A	T	1: 165,855,190	D28V	probably damaging	Het
Celsr2	T	A	3: 108,413,126	N790I	probably damaging	Het
Clrn1	C	T	3: 58,884,830	V71I	probably benign	Het
Cmtr2	T	C	8: 110,222,446	Y463H	possibly damaging	Het
Col6a6	T	C	9: 105,785,973	T122A	probably benign	Het
Cubn	T	A	2: 13,458,956	D646V	probably damaging	Het
Dab2ip	T	A	2: 35,723,093	L1062Q	possibly damaging	Het
Dnajb4	T	C	3: 152,186,635	N187S	probably benign	Het
Dock10	A	T	1: 80,592,643	F365L	probably benign	Het
Erich6	G	A	3: 58,626,680	Q309*	probably null	Het
Ext1	T	C	15: 53,345,128	H79R	probably benign	Het
Fam155a	C	A	8: 9,771,006	A5S	probably damaging	Het
Fam187b	T	A	7: 30,977,760	C231*	probably null	Het
Fbxo15	T	A	18: 84,965,484	M319K	unknown	Het
Fbxo18	T	C	2: 11,749,603	I775V	probably benign	Het
Fbxo9	A	T	9: 78,095,238	M187K	possibly damaging	Het
Fer1l4	T	C	2: 156,035,276	D1113G	probably benign	Het
Fsip2	A	T	2: 82,988,403	T4827S	possibly damaging	Het
Gm49398	A	T	13: 61,287,664	L7Q	probably damaging	Het
H2-M10.4	A	T	17: 36,460,393	W298R	probably damaging	Het
Hoxd10	A	G	2: 74,692,638	E220G	probably benign	Het
Hrh2	T	G	13: 54,214,203	L66R	probably damaging	Het
Igf1r	A	G	7: 68,194,998	T840A	probably benign	Het
Iqca	A	G	1: 90,144,966	V64A	probably damaging	Het
Klf15	A	G	6: 90,466,869	E142G	probably damaging	Het
Krt40	T	A	11: 99,538,753	T332S	probably damaging	Het
Lrrc9	T	C	12: 72,459,993	F348S	probably damaging	Het
Lrrn4	T	C	2: 132,870,370	D511G	probably benign	Het
Lrtm2	T	C	6: 119,320,973	I36V	probably benign	Het
Lsm10	T	C	4: 126,098,067	L72P	probably damaging	Het
Mab21l3	T	A	3: 101,835,203	S14C	possibly damaging	Het
Map2k4	T	C	11: 65,690,743	K381R	probably benign	Het
Mcidas	A	G	13: 112,994,381	D80G	probably damaging	Het
Med23	A	G	10: 24,874,571	M99V	probably benign	Het
Mga	A	G	2: 119,948,175	D2067G	probably benign	Het
Msc	G	T	1: 14,755,483	P89Q	probably benign	Het
Olfr1086	A	T	2: 86,677,150	L61Q	probably damaging	Het
Olfr301	A	G	7: 86,413,222	I287V	probably benign	Het
Olfr709-ps1	A	T	7: 106,927,357	I34N	possibly damaging	Het
Plekhg6	T	C	6: 125,363,060	D779G	probably damaging	Het
Pnpla2	C	A	7: 141,455,418	Y44*	probably null	Het
Ppp1r17	T	A	6: 56,026,539	D112E	probably damaging	Het
Prdm2	A	G	4: 143,134,908	I604T	probably damaging	Het
Prkce	A	G	17: 86,474,449	Y182C	probably damaging	Het
Prokr2	G	A	2: 132,340,870	S189F	possibly damaging	Het
Qser1	A	T	2: 104,787,819	S793T	probably benign	Het
Reck	T	C	4: 43,943,301	F951S	probably benign	Het
Rev3l	T	C	10: 39,821,462	S652P	probably benign	Het
Rtl1	A	C	12: 109,592,450	L985R	probably damaging	Het
Sardh	T	C	2: 27,230,857	E385G	possibly damaging	Het
Sfmbt1	G	T	14: 30,797,642	W440L	possibly damaging	Het
Slc4a7	T	A	14: 14,772,541	C683*	probably null	Het
Slc51a	A	G	16: 32,479,699	L80P	possibly damaging	Het
Slco3a1	C	A	7: 74,504,289	L178F	probably damaging	Het
Spata6	T	A	4: 111,779,192	C227S	possibly damaging	Het
Tbc1d30	A	T	10: 121,267,461	Y555*	probably null	Het
Tcf7l2	A	G	19: 55,743,085	Q90R	probably benign	Het
Tff3	A	C	17: 31,127,449	S50A	probably benign	Het
Tnrc6c	T	C	11: 117,739,894	M1027T	probably benign	Het
Ttc12	A	G	9: 49,440,380	F606L	probably benign	Het
Uqcc3	G	A	19: 8,880,726	Q34*	probably null	Het
Veph1	T	A	3: 66,244,538	I157F	probably damaging	Het
Vmn2r14	G	A	5: 109,220,562	T188I	probably damaging	Het
Vmn2r67	A	G	7: 85,136,580	I739T	probably benign	Het
Vmn2r68	A	G	7: 85,233,785	F253S	probably benign	Het
Vmn2r74	T	C	7: 85,957,416	I241V	probably benign	Het
Vmn2r97	A	G	17: 18,929,106	E252G	probably benign	Het
Vps13b	T	C	15: 35,455,054	V703A	possibly damaging	Het
Vps25	T	A	11: 101,258,797	L149Q	probably damaging	Het
Xirp1	T	C	9: 120,016,884	T978A	probably benign	Het
Xpnpep1	T	A	19: 53,004,817	I360F	probably benign	Het
Zfpm1	C	T	8: 122,334,569	T291M	probably benign	Het
Zkscan8	A	G	13: 21,526,532	V136A	probably benign	Het
Zscan18	A	G	7: 12,771,685	Y592H	probably damaging	Het

Other mutations in Klc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00787:Klc2	APN	19	5111662	missense	probably benign	0.17
IGL00822:Klc2	APN	19	5111513	missense	probably damaging	1.00
IGL01732:Klc2	APN	19	5109797	missense	probably damaging	1.00
IGL02374:Klc2	APN	19	5110410	missense	possibly damaging	0.76
IGL02677:Klc2	APN	19	5111668	missense	probably damaging	1.00
P0042:Klc2	UTSW	19	5113777	unclassified	probably benign
R0126:Klc2	UTSW	19	5112746	missense	possibly damaging	0.93
R1687:Klc2	UTSW	19	5111654	missense	probably damaging	1.00
R1887:Klc2	UTSW	19	5108612	missense	probably benign	0.00
R5620:Klc2	UTSW	19	5112856	missense	probably damaging	1.00
R6977:Klc2	UTSW	19	5109365	missense	probably damaging	1.00
R7622:Klc2	UTSW	19	5111632	missense	probably damaging	0.96
R7631:Klc2	UTSW	19	5108619	missense	probably benign	0.21
R8017:Klc2	UTSW	19	5111839	missense	probably benign
R8339:Klc2	UTSW	19	5109534	missense	probably benign	0.44
R8737:Klc2	UTSW	19	5118449	unclassified	probably benign
R8830:Klc2	UTSW	19	5110366	critical splice donor site	probably null
R8962:Klc2	UTSW	19	5111836	missense	probably benign	0.05
R9435:Klc2	UTSW	19	5109634	missense	possibly damaging	0.80
R9532:Klc2	UTSW	19	5111537	missense	possibly damaging	0.50

Predicted Primers

PCR Primer
(F):5'- GCCATGGCTGAGATGACAAG -3'
(R):5'- GGTCCTGGTTGTGAGCTAAAC -3'

Sequencing Primer
(F):5'- GGGAGGAAGTTACCCTGAGATTG -3'
(R):5'- GTCCTGGTTGTGAGCTAAACTTTAAC -3'

Posted On

2022-04-18