Mutagenetix > Incidental Mutation

Incidental Mutation 'R9344:Gsto2'

707713

Institutional Source

Beutler Lab

Gene Symbol

Gsto2

Ensembl Gene

ENSMUSG00000025069

Gene Name

glutathione S-transferase omega 2

Synonyms

4930425C18Rik, 1700020F09Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9344 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

47853973-47874763 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 47864884 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 139 (D139G)

Ref Sequence

ENSEMBL: ENSMUSP00000052592 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056159] [ENSMUST00000120645] [ENSMUST00000135016]

AlphaFold

Q8K2Q2

Predicted Effect

probably benign
Transcript: ENSMUST00000056159
AA Change: D139G

PolyPhen 2 Score 0.254 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000052592
Gene: ENSMUSG00000025069
AA Change: D139G

Domain	Start	End	E-Value	Type
Pfam:GST_N	23	95	1.7e-9	PFAM
Pfam:Glutaredoxin	24	75	1.1e-7	PFAM
Pfam:GST_N_3	26	101	1.9e-21	PFAM
Pfam:GST_N_2	31	96	3.2e-14	PFAM
SCOP:d1eema1	104	242	3e-25	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000120645
AA Change: D139G

PolyPhen 2 Score 0.254 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000113409
Gene: ENSMUSG00000025069
AA Change: D139G

Domain	Start	End	E-Value	Type
Pfam:GST_N	22	95	3.8e-14	PFAM
Pfam:Glutaredoxin	24	75	9e-8	PFAM
Pfam:GST_N_3	26	101	6.9e-22	PFAM
Pfam:GST_N_2	31	96	1.7e-14	PFAM
SCOP:d1eema1	104	242	3e-25	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135016
AA Change: D139G

PolyPhen 2 Score 0.254 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000119680
Gene: ENSMUSG00000025069
AA Change: D139G

Domain	Start	End	E-Value	Type
Pfam:GST_N	23	95	3.4e-10	PFAM
Pfam:Glutaredoxin	24	75	4.4e-8	PFAM
Pfam:GST_N_3	26	101	4.2e-22	PFAM
Pfam:GST_N_2	31	96	6e-15	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (57/57)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an omega class glutathione S-transferase (GST). GSTs are involved in the metabolism of xenobiotics and carcinogens. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930453N24Rik	A	G	16: 64,591,135 (GRCm39)	V31A	possibly damaging	Het
Adss2	A	G	1: 177,597,527 (GRCm39)	Y378H	probably damaging	Het
Aldh1a1	T	A	19: 20,608,150 (GRCm39)	V324D	probably damaging	Het
Ankrd33b	A	G	15: 31,297,903 (GRCm39)	S285P	probably damaging	Het
Arel1	C	T	12: 84,981,371 (GRCm39)	G269S	probably damaging	Het
Arhgap44	A	G	11: 65,053,463 (GRCm39)	M1T	probably null	Het
Arpin	C	T	7: 79,577,983 (GRCm39)	V149I	probably benign	Het
Arpp21	A	G	9: 112,014,720 (GRCm39)	L28P	possibly damaging	Het
Ccr8	G	A	9: 119,923,133 (GRCm39)	V83I	probably damaging	Het
Cnksr1	T	C	4: 133,963,508 (GRCm39)	E58G	probably damaging	Het
Dctn2	T	A	10: 127,114,084 (GRCm39)	H341Q	probably damaging	Het
Ddx21	C	A	10: 62,428,825 (GRCm39)	A362S	possibly damaging	Het
Dnajc1	C	T	2: 18,289,586 (GRCm39)	V274I	probably benign	Het
Dock3	A	C	9: 106,870,763 (GRCm39)	C550W	probably damaging	Het
Dst	T	C	1: 34,220,676 (GRCm39)	L2160S	probably damaging	Het
Duox1	T	A	2: 122,168,163 (GRCm39)	M1096K	probably benign	Het
Dync2h1	A	T	9: 7,148,659 (GRCm39)	D928E	probably benign	Het
E030025P04Rik	C	T	11: 109,030,454 (GRCm39)		probably null	Het
Eid2b	T	C	7: 27,977,591 (GRCm39)	M129T	possibly damaging	Het
Fnip2	G	A	3: 79,407,717 (GRCm39)	S288F	possibly damaging	Het
Grm4	G	T	17: 27,653,737 (GRCm39)	R738S	probably benign	Het
Herc2	T	A	7: 55,772,112 (GRCm39)	V1097E	probably benign	Het
Impg1	G	A	9: 80,312,040 (GRCm39)	A181V	probably benign	Het
Irs2	G	T	8: 11,057,289 (GRCm39)	S381*	probably null	Het
Itprid1	C	T	6: 55,955,470 (GRCm39)	T1026I	probably benign	Het
Lrrd1	A	C	5: 3,908,819 (GRCm39)	D697A	possibly damaging	Het
Nat10	A	G	2: 103,573,460 (GRCm39)	S346P	probably damaging	Het
Ncapg2	G	A	12: 116,388,273 (GRCm39)	R319H	probably damaging	Het
Nid1	A	G	13: 13,652,894 (GRCm39)	Y568C	probably damaging	Het
Noc2l	G	A	4: 156,325,130 (GRCm39)	C325Y	probably damaging	Het
Or12e9	A	T	2: 87,202,161 (GRCm39)	D95V	possibly damaging	Het
Or1e17	C	A	11: 73,831,744 (GRCm39)	S224Y	possibly damaging	Het
Pde2a	G	T	7: 101,144,891 (GRCm39)	V169F	possibly damaging	Het
Pon3	T	C	6: 5,221,586 (GRCm39)	K348R	probably benign	Het
Ppp6c	A	G	2: 39,090,052 (GRCm39)		probably null	Het
Prrc2b	G	A	2: 32,103,600 (GRCm39)	G1026D	probably benign	Het
Psg22	A	T	7: 18,460,816 (GRCm39)	T482S	possibly damaging	Het
Rdh19	G	A	10: 127,692,740 (GRCm39)	V136M	probably damaging	Het
Sbf2	T	A	7: 109,940,535 (GRCm39)	N1275I	probably benign	Het
Sema4c	T	C	1: 36,592,395 (GRCm39)	N182D	probably damaging	Het
Slc7a12	T	A	3: 14,570,491 (GRCm39)	H414Q	probably damaging	Het
Slitrk5	A	G	14: 111,916,702 (GRCm39)	I109V	probably damaging	Het
Spag17	C	T	3: 100,010,793 (GRCm39)	P2096S	probably benign	Het
Steap1	T	A	5: 5,786,459 (GRCm39)	D326V	probably damaging	Het
Tacr1	C	T	6: 82,380,847 (GRCm39)	T86I	probably damaging	Het
Tenm4	C	T	7: 96,545,352 (GRCm39)	T2493I	probably damaging	Het
Tet2	A	G	3: 133,175,115 (GRCm39)	S1411P	possibly damaging	Het
Tom1	C	T	8: 75,785,076 (GRCm39)	R303C	probably damaging	Het
Trav7n-4	T	A	14: 53,329,200 (GRCm39)	I70N	possibly damaging	Het
Utrn	A	C	10: 12,560,275 (GRCm39)	V1338G	probably benign	Het
Vipr1	G	A	9: 121,471,993 (GRCm39)		probably null	Het
Vmn1r122	A	T	7: 20,867,271 (GRCm39)	H261Q	probably benign	Het
Vmn1r40	G	T	6: 89,691,235 (GRCm39)	L17F	probably benign	Het
Vmn2r54	A	G	7: 12,366,283 (GRCm39)	V217A	probably benign	Het
Vmn2r55	C	A	7: 12,385,782 (GRCm39)	G733*	probably null	Het
Vmn2r98	A	T	17: 19,286,777 (GRCm39)	N425I	probably benign	Het
Vps51	C	T	19: 6,126,345 (GRCm39)	V136I	unknown	Het
Zfp184	T	C	13: 22,144,411 (GRCm39)	C706R	probably damaging	Het

Other mutations in Gsto2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00161:Gsto2	APN	19	47,863,406 (GRCm39)	missense	probably damaging	1.00
IGL02561:Gsto2	APN	19	47,874,629 (GRCm39)	unclassified	probably benign
IGL02820:Gsto2	APN	19	47,863,398 (GRCm39)	missense	probably damaging	1.00
IGL03141:Gsto2	APN	19	47,863,312 (GRCm39)	missense	probably damaging	1.00
R1343:Gsto2	UTSW	19	47,873,146 (GRCm39)	splice site	probably null
R4297:Gsto2	UTSW	19	47,864,935 (GRCm39)	missense	possibly damaging	0.49
R4427:Gsto2	UTSW	19	47,860,212 (GRCm39)	missense	possibly damaging	0.94
R4701:Gsto2	UTSW	19	47,873,095 (GRCm39)	missense	probably benign	0.02
R4762:Gsto2	UTSW	19	47,863,312 (GRCm39)	missense	probably damaging	1.00
R6765:Gsto2	UTSW	19	47,860,227 (GRCm39)	nonsense	probably null
R7903:Gsto2	UTSW	19	47,873,096 (GRCm39)	missense	possibly damaging	0.50
R8877:Gsto2	UTSW	19	47,873,176 (GRCm39)	missense	probably damaging	1.00
R8939:Gsto2	UTSW	19	47,873,203 (GRCm39)	critical splice donor site	probably null
R9351:Gsto2	UTSW	19	47,874,608 (GRCm39)	missense	possibly damaging	0.95
R9477:Gsto2	UTSW	19	47,864,911 (GRCm39)	missense	probably benign	0.01
X0018:Gsto2	UTSW	19	47,863,340 (GRCm39)	missense	probably benign	0.12
X0067:Gsto2	UTSW	19	47,874,461 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGCTACAGTCTCTAGGCACAGAG -3'
(R):5'- TTTCAGAGCAGGTCAGGGAC -3'

Sequencing Primer
(F):5'- GTCTCTAGGCACAGAGATCCC -3'
(R):5'- AGGTCAGGGACTCAGGCATC -3'

Posted On

2022-04-18