Mutagenetix > Incidental Mutation

Incidental Mutation 'R9351:Gsto2'

708103

Institutional Source

Beutler Lab

Gene Symbol

Gsto2

Ensembl Gene

ENSMUSG00000025069

Gene Name

glutathione S-transferase omega 2

Synonyms

4930425C18Rik, 1700020F09Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9351 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

47853973-47874763 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 47874608 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 243 (C243S)

Ref Sequence

ENSEMBL: ENSMUSP00000052592 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056159] [ENSMUST00000120645] [ENSMUST00000135016]

AlphaFold

Q8K2Q2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000056159
AA Change: C243S

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000052592
Gene: ENSMUSG00000025069
AA Change: C243S

Domain	Start	End	E-Value	Type
Pfam:GST_N	23	95	1.7e-9	PFAM
Pfam:Glutaredoxin	24	75	1.1e-7	PFAM
Pfam:GST_N_3	26	101	1.9e-21	PFAM
Pfam:GST_N_2	31	96	3.2e-14	PFAM
SCOP:d1eema1	104	242	3e-25	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000120645
AA Change: C243S

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000113409
Gene: ENSMUSG00000025069
AA Change: C243S

Domain	Start	End	E-Value	Type
Pfam:GST_N	22	95	3.8e-14	PFAM
Pfam:Glutaredoxin	24	75	9e-8	PFAM
Pfam:GST_N_3	26	101	6.9e-22	PFAM
Pfam:GST_N_2	31	96	1.7e-14	PFAM
SCOP:d1eema1	104	242	3e-25	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135016

SMART Domains

Protein: ENSMUSP00000119680
Gene: ENSMUSG00000025069

Domain	Start	End	E-Value	Type
Pfam:GST_N	23	95	3.4e-10	PFAM
Pfam:Glutaredoxin	24	75	4.4e-8	PFAM
Pfam:GST_N_3	26	101	4.2e-22	PFAM
Pfam:GST_N_2	31	96	6e-15	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

100% (54/54)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an omega class glutathione S-transferase (GST). GSTs are involved in the metabolism of xenobiotics and carcinogens. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	A	G	10: 87,066,068 (GRCm39)	T84A	probably benign	Het
Abca17	G	A	17: 24,510,751 (GRCm39)	S909L	probably benign	Het
Adamtsl3	A	T	7: 82,169,929 (GRCm39)	Y371F	possibly damaging	Het
Adcy9	A	T	16: 4,236,228 (GRCm39)	S394R	probably damaging	Het
Ahcyl1	T	C	3: 107,575,011 (GRCm39)	N444S	probably damaging	Het
Ahnak	A	C	19: 8,985,232 (GRCm39)	D2172A	probably damaging	Het
Arhgef28	C	T	13: 98,130,576 (GRCm39)	D421N	probably benign	Het
Brd1	T	A	15: 88,614,307 (GRCm39)	E196V	possibly damaging	Het
Cabs1	A	G	5: 88,128,300 (GRCm39)	D317G	probably damaging	Het
Ccdc96	T	C	5: 36,642,069 (GRCm39)	I25T	unknown	Het
Clgn	A	G	8: 84,153,218 (GRCm39)	R607G	possibly damaging	Het
Clp1	T	C	2: 84,554,195 (GRCm39)	K325E	probably benign	Het
Dcun1d1	A	T	3: 35,975,185 (GRCm39)	I52K	probably benign	Het
Dync2h1	G	A	9: 7,176,911 (GRCm39)	T16I	probably damaging	Het
Eif4a3l1	A	T	6: 136,306,771 (GRCm39)	I411F	probably benign	Het
Epb41l5	A	G	1: 119,477,639 (GRCm39)	F711L	probably benign	Het
Esr1	G	A	10: 4,696,763 (GRCm39)	W204*	probably null	Het
Fat2	A	G	11: 55,172,127 (GRCm39)	L2862P	probably damaging	Het
Frmd3	A	G	4: 74,054,068 (GRCm39)	E159G	probably damaging	Het
Heg1	A	G	16: 33,545,867 (GRCm39)	H206R	probably benign	Het
Ifi203	A	G	1: 173,750,133 (GRCm39)	V862A	probably benign	Het
Ippk	C	T	13: 49,615,107 (GRCm39)	H497Y	probably benign	Het
Irf5	A	T	6: 29,531,317 (GRCm39)	N61I	possibly damaging	Het
Iws1	G	A	18: 32,213,213 (GRCm39)	E214K	possibly damaging	Het
Kcnj12	C	T	11: 60,960,673 (GRCm39)	H324Y	probably damaging	Het
Kdm2a	C	T	19: 4,393,141 (GRCm39)	D405N		Het
Lars2	A	G	9: 123,265,366 (GRCm39)	Q474R	probably benign	Het
Lctl	T	C	9: 64,040,473 (GRCm39)	F472S	possibly damaging	Het
Map4k2	T	C	19: 6,401,223 (GRCm39)	S590P	probably benign	Het
Mcf2l	G	T	8: 13,050,757 (GRCm39)	L337F	possibly damaging	Het
Mvp	A	G	7: 126,595,435 (GRCm39)	V225A	probably damaging	Het
Nfat5	T	A	8: 108,065,910 (GRCm39)	D241E	probably damaging	Het
Or6n2	T	A	1: 173,897,021 (GRCm39)	D52E	probably benign	Het
Pagr1a	A	T	7: 126,616,073 (GRCm39)	H7Q	probably damaging	Het
Parp8	G	T	13: 117,000,781 (GRCm39)	Q787K	probably damaging	Het
Pcdhb2	C	A	18: 37,429,369 (GRCm39)	N90K	probably damaging	Het
Pde6h	A	G	6: 136,936,332 (GRCm39)	K25R	probably benign	Het
Prr35	G	A	17: 26,166,118 (GRCm39)	Q390*	probably null	Het
Psd3	C	A	8: 68,413,301 (GRCm39)	A410S	probably benign	Het
Pus10	A	G	11: 23,617,311 (GRCm39)	N8S	probably benign	Het
Rnf115	T	C	3: 96,695,994 (GRCm39)	L260S	probably damaging	Het
Sdsl	A	T	5: 120,601,159 (GRCm39)	Y38N	probably benign	Het
Sf1	A	G	19: 6,415,694 (GRCm39)	D11G	probably damaging	Het
Stxbp5l	A	G	16: 36,936,047 (GRCm39)	Y1177H	probably damaging	Het
Taar2	G	A	10: 23,816,900 (GRCm39)	V147I	probably benign	Het
Tmed3	A	T	9: 89,584,980 (GRCm39)	F92I	possibly damaging	Het
Tmprss15	T	C	16: 78,832,086 (GRCm39)	T357A	probably damaging	Het
Trbv14	A	T	6: 41,112,428 (GRCm39)	D75V	probably damaging	Het
Vmn1r30	A	G	6: 58,412,262 (GRCm39)	V190A	probably benign	Het
Vmn2r115	A	T	17: 23,578,482 (GRCm39)	I652F	probably benign	Het
Vmn2r26	A	T	6: 124,016,333 (GRCm39)	M266L	probably benign	Het
Wdr17	T	C	8: 55,143,057 (GRCm39)	I198V	probably benign	Het
Wdr27	G	A	17: 15,128,833 (GRCm39)	A540V	possibly damaging	Het
Zfp26	A	C	9: 20,349,447 (GRCm39)	Y372*	probably null	Het
Zfp286	A	G	11: 62,670,801 (GRCm39)	V424A	probably damaging	Het

Other mutations in Gsto2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00161:Gsto2	APN	19	47,863,406 (GRCm39)	missense	probably damaging	1.00
IGL02561:Gsto2	APN	19	47,874,629 (GRCm39)	unclassified	probably benign
IGL02820:Gsto2	APN	19	47,863,398 (GRCm39)	missense	probably damaging	1.00
IGL03141:Gsto2	APN	19	47,863,312 (GRCm39)	missense	probably damaging	1.00
R1343:Gsto2	UTSW	19	47,873,146 (GRCm39)	splice site	probably null
R4297:Gsto2	UTSW	19	47,864,935 (GRCm39)	missense	possibly damaging	0.49
R4427:Gsto2	UTSW	19	47,860,212 (GRCm39)	missense	possibly damaging	0.94
R4701:Gsto2	UTSW	19	47,873,095 (GRCm39)	missense	probably benign	0.02
R4762:Gsto2	UTSW	19	47,863,312 (GRCm39)	missense	probably damaging	1.00
R6765:Gsto2	UTSW	19	47,860,227 (GRCm39)	nonsense	probably null
R7903:Gsto2	UTSW	19	47,873,096 (GRCm39)	missense	possibly damaging	0.50
R8877:Gsto2	UTSW	19	47,873,176 (GRCm39)	missense	probably damaging	1.00
R8939:Gsto2	UTSW	19	47,873,203 (GRCm39)	critical splice donor site	probably null
R9344:Gsto2	UTSW	19	47,864,884 (GRCm39)	missense	probably benign	0.25
R9477:Gsto2	UTSW	19	47,864,911 (GRCm39)	missense	probably benign	0.01
X0018:Gsto2	UTSW	19	47,863,340 (GRCm39)	missense	probably benign	0.12
X0067:Gsto2	UTSW	19	47,874,461 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GTGATAACTGTCCTCGTCCC -3'
(R):5'- TGCTGGGCACTTTCTAGAGAATC -3'

Sequencing Primer
(F):5'- GTCCTCGTCCCCACCCTG -3'
(R):5'- GCACTTTCTAGAGAATCATTAGAGGC -3'

Posted On

2022-04-18