Mutagenetix > Incidental Mutation

Incidental Mutation 'R9352:Unc13b'

708120

Institutional Source

Beutler Lab

Gene Symbol

Unc13b

Ensembl Gene

ENSMUSG00000028456

Gene Name

unc-13 homolog B

Synonyms

Munc13-2, Unc13h2

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.262) question?

Stock #

R9352 (G1)

Quality Score

185.468

Status

Not validated

Chromosome

Chromosomal Location

43058953-43264871 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

GAGCCA to GAGCCATAGCCA at 43177313 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000147100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079978] [ENSMUST00000107952] [ENSMUST00000107953] [ENSMUST00000163653] [ENSMUST00000207569]

AlphaFold

Q9Z1N9

Predicted Effect

probably benign
Transcript: ENSMUST00000079978

SMART Domains

Protein: ENSMUSP00000078894
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	179	193	N/A	INTRINSIC
low complexity region	292	303	N/A	INTRINSIC
low complexity region	322	333	N/A	INTRINSIC
C1	478	527	4.21e-18	SMART
C2	601	708	2.07e-22	SMART
DUF1041	917	1021	2.02e-53	SMART
Pfam:Membr_traf_MHD	1262	1404	4.8e-60	PFAM
C2	1438	1544	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107952

SMART Domains

Protein: ENSMUSP00000103586
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	191	205	N/A	INTRINSIC
low complexity region	304	315	N/A	INTRINSIC
low complexity region	334	345	N/A	INTRINSIC
C1	490	539	4.21e-18	SMART
C2	613	720	2.07e-22	SMART
DUF1041	929	1033	2.02e-53	SMART
Pfam:Membr_traf_MHD	1274	1416	4.8e-60	PFAM
C2	1450	1556	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107953

SMART Domains

Protein: ENSMUSP00000103587
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	179	193	N/A	INTRINSIC
low complexity region	292	303	N/A	INTRINSIC
low complexity region	322	333	N/A	INTRINSIC
C1	478	527	4.21e-18	SMART
C2	601	708	2.07e-22	SMART
DUF1041	917	1021	2.02e-53	SMART
Pfam:Membr_traf_MHD	1263	1403	2.3e-56	PFAM
C2	1457	1563	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000163653

SMART Domains

Protein: ENSMUSP00000128608
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	191	205	N/A	INTRINSIC
low complexity region	304	315	N/A	INTRINSIC
low complexity region	334	345	N/A	INTRINSIC
C1	490	539	4.21e-18	SMART
C2	613	720	2.07e-22	SMART
DUF1041	929	1032	4.64e-53	SMART
Pfam:Membr_traf_MHD	1273	1415	4.8e-60	PFAM
C2	1449	1555	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000168032

SMART Domains

Protein: ENSMUSP00000132622
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C1	147	196	4.21e-18	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000207569

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice are grossly phenotypically normal. Mice older than 12 months will exhibit sporadic seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamtsl3	A	T	7: 82,091,656 (GRCm39)	Q123L	probably damaging	Het
Adarb2	T	A	13: 8,807,428 (GRCm39)	L743Q	probably damaging	Het
Alpk2	C	A	18: 65,439,783 (GRCm39)	D537Y	probably benign	Het
Anxa4	C	T	6: 86,742,775 (GRCm39)		probably benign	Het
Arfgap3	T	A	15: 83,191,127 (GRCm39)	I464L	possibly damaging	Het
Asb2	C	T	12: 103,296,698 (GRCm39)	V320I	probably damaging	Het
Axl	T	C	7: 25,462,752 (GRCm39)	T659A	possibly damaging	Het
Bbof1	A	G	12: 84,461,394 (GRCm39)	H139R	probably benign	Het
Birc6	G	A	17: 74,965,347 (GRCm39)		probably null	Het
Cdh23	G	A	10: 60,143,306 (GRCm39)	A3005V	possibly damaging	Het
Chil3	A	T	3: 106,067,787 (GRCm39)	F126Y	probably damaging	Het
Chrnb4	T	A	9: 54,951,167 (GRCm39)	D32V	probably benign	Het
Chst15	C	A	7: 131,872,257 (GRCm39)	C8F	probably damaging	Het
Cntnap5c	A	G	17: 58,399,463 (GRCm39)	I439V	probably benign	Het
Crisp2	T	A	17: 41,078,200 (GRCm39)	N194I	probably damaging	Het
Csrnp1	G	A	9: 119,801,997 (GRCm39)	T354M	probably benign	Het
Cul1	T	A	6: 47,479,426 (GRCm39)	S231T	probably benign	Het
Cyp27a1	C	T	1: 74,752,920 (GRCm39)	T44M	possibly damaging	Het
Dgkg	T	C	16: 22,398,581 (GRCm39)	D232G	probably damaging	Het
Dnah1	G	T	14: 31,038,620 (GRCm39)	Q154K	probably benign	Het
Dnmt1	A	G	9: 20,840,384 (GRCm39)	V304A	probably benign	Het
Dok6	T	C	18: 89,492,133 (GRCm39)	N148S	probably benign	Het
Fnta	A	T	8: 26,501,119 (GRCm39)	W134R	probably damaging	Het
Garin5b	T	A	7: 4,761,605 (GRCm39)	Y369F		Het
Gm32742	T	G	9: 51,052,544 (GRCm39)	Q1441P	possibly damaging	Het
Gm3486	T	C	14: 41,208,318 (GRCm39)	Q131R	possibly damaging	Het
Gnptab	A	G	10: 88,268,350 (GRCm39)	N486D	probably benign	Het
H2-Q4	T	G	17: 35,601,909 (GRCm39)	F257C	probably damaging	Het
Hfe	A	G	13: 23,890,119 (GRCm39)	V218A	probably benign	Het
Htr2b	A	G	1: 86,027,294 (GRCm39)	V404A	probably benign	Het
Hyou1	T	A	9: 44,300,926 (GRCm39)		probably null	Het
Ikbkb	T	C	8: 23,150,444 (GRCm39)	D746G	probably benign	Het
Ikzf4	T	C	10: 128,472,623 (GRCm39)	N251S	probably benign	Het
Il23r	A	C	6: 67,403,592 (GRCm39)	N436K	probably damaging	Het
Impg2	A	T	16: 56,072,470 (GRCm39)	I301L	probably benign	Het
Irf4	A	T	13: 30,936,706 (GRCm39)	M146L	probably benign	Het
Iws1	G	A	18: 32,213,213 (GRCm39)	E214K	possibly damaging	Het
Kctd3	T	C	1: 188,704,777 (GRCm39)	S665G	probably damaging	Het
Kiz	T	C	2: 146,794,927 (GRCm39)	S596P	probably damaging	Het
Kpna2	T	C	11: 106,880,292 (GRCm39)	E452G	probably damaging	Het
Lclat1	A	G	17: 73,468,937 (GRCm39)	Y39C	probably damaging	Het
Lig3	A	G	11: 82,686,971 (GRCm39)	S705G	probably benign	Het
Lrp1b	T	C	2: 40,748,438 (GRCm39)	D3134G		Het
Map3k8	C	T	18: 4,349,170 (GRCm39)	M49I	probably benign	Het
Mep1b	A	G	18: 21,209,431 (GRCm39)	D54G	probably damaging	Het
Mgat5b	T	A	11: 116,857,533 (GRCm39)	S342R	probably benign	Het
Mthfd2l	G	T	5: 91,109,172 (GRCm39)	V201L	possibly damaging	Het
Naip6	G	T	13: 100,437,893 (GRCm39)	T371K	possibly damaging	Het
Naxd	T	C	8: 11,555,504 (GRCm39)	I104T	probably damaging	Het
Nbeal2	T	A	9: 110,456,916 (GRCm39)	I2361F	probably benign	Het
Ogfr	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	2: 180,237,059 (GRCm39)		probably benign	Het
Or1e17	A	G	11: 73,831,470 (GRCm39)	T133A	probably benign	Het
Or6c75	A	T	10: 129,337,364 (GRCm39)	M196L	probably benign	Het
Or8b8	A	G	9: 37,808,712 (GRCm39)	E4G	probably benign	Het
Or8c16	T	G	9: 38,130,683 (GRCm39)	L185R	probably damaging	Het
Pga5	C	T	19: 10,646,897 (GRCm39)	G303S	probably damaging	Het
Polq	T	C	16: 36,862,252 (GRCm39)	F591L	probably damaging	Het
Ppp2r1a	A	T	17: 21,185,499 (GRCm39)		probably null	Het
Prrc1	A	G	18: 57,522,317 (GRCm39)	Y383C	probably damaging	Het
Ptgfr	A	T	3: 151,541,160 (GRCm39)	V116D	probably damaging	Het
Qpctl	C	T	7: 18,878,599 (GRCm39)	R292Q	possibly damaging	Het
Rasip1	G	A	7: 45,278,280 (GRCm39)	V194M	possibly damaging	Het
Rbbp4	A	T	4: 129,211,498 (GRCm39)	N385K	probably benign	Het
Rbbp8nl	A	G	2: 179,921,053 (GRCm39)	S444P	probably benign	Het
Resf1	T	C	6: 149,236,180 (GRCm39)	F1500S	probably damaging	Het
Sf3a1	G	A	11: 4,110,494 (GRCm39)	A3T	unknown	Het
Slc1a4	A	G	11: 20,282,025 (GRCm39)	S150P	probably damaging	Het
Slc28a2	T	C	2: 122,281,522 (GRCm39)		probably null	Het
Slc30a5	A	G	13: 100,940,380 (GRCm39)	I702T	probably benign	Het
Slc36a4	T	A	9: 15,633,319 (GRCm39)		probably null	Het
Slf2	A	G	19: 44,931,957 (GRCm39)	R671G	probably null	Het
Smarcc1	A	G	9: 110,035,220 (GRCm39)	K881R	probably null	Het
Srsf11	A	G	3: 157,717,836 (GRCm39)	V414A	unknown	Het
Tcn2	T	C	11: 3,873,446 (GRCm39)	N300S	probably damaging	Het
Tecta	T	C	9: 42,249,147 (GRCm39)	K1905R	probably damaging	Het
Tex30	T	G	1: 44,130,753 (GRCm39)		probably null	Het
Tktl2	A	T	8: 66,965,974 (GRCm39)	I511F	possibly damaging	Het
Tpp1	G	T	7: 105,398,881 (GRCm39)	Q183K	probably benign	Het
Trib2	A	C	12: 15,865,413 (GRCm39)	I30R	probably benign	Het
Tti1	A	G	2: 157,842,692 (GRCm39)	L779P	probably benign	Het
Vmn2r112	A	G	17: 22,822,479 (GRCm39)	T386A	probably damaging	Het
Vps16	T	C	2: 130,283,823 (GRCm39)		probably null	Het
Zan	T	G	5: 137,434,745 (GRCm39)	N2186T	unknown	Het
Zdhhc11	C	T	13: 74,121,800 (GRCm39)	R104*	probably null	Het
Zfp318	T	G	17: 46,721,284 (GRCm39)	H1176Q	probably damaging	Het
Zfp335	G	A	2: 164,742,242 (GRCm39)	H581Y	probably damaging	Het
Zfp7	T	C	15: 76,775,674 (GRCm39)	I572T	probably damaging	Het
Zfp955a	T	C	17: 33,461,335 (GRCm39)	T266A	probably benign	Het

Other mutations in Unc13b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Unc13b	APN	4	43,240,285 (GRCm39)	missense	probably damaging	1.00
IGL00832:Unc13b	APN	4	43,258,921 (GRCm39)	missense	probably damaging	1.00
IGL01111:Unc13b	APN	4	43,096,927 (GRCm39)	missense	possibly damaging	0.76
IGL01115:Unc13b	APN	4	43,258,492 (GRCm39)	missense	probably damaging	1.00
IGL01137:Unc13b	APN	4	43,091,291 (GRCm39)	missense	probably damaging	1.00
IGL01637:Unc13b	APN	4	43,241,066 (GRCm39)	missense	probably damaging	1.00
IGL01789:Unc13b	APN	4	43,239,462 (GRCm39)	missense	probably damaging	1.00
IGL01792:Unc13b	APN	4	43,250,218 (GRCm39)	missense	probably damaging	0.99
IGL01877:Unc13b	APN	4	43,249,583 (GRCm39)	critical splice donor site	probably null
IGL01924:Unc13b	APN	4	43,239,385 (GRCm39)	nonsense	probably null
IGL02087:Unc13b	APN	4	43,091,270 (GRCm39)	missense	probably null	1.00
IGL02197:Unc13b	APN	4	43,165,828 (GRCm39)	missense	probably damaging	0.99
IGL02504:Unc13b	APN	4	43,263,031 (GRCm39)	missense	probably damaging	1.00
IGL02659:Unc13b	APN	4	43,235,332 (GRCm39)	missense	probably damaging	1.00
IGL03031:Unc13b	APN	4	43,235,368 (GRCm39)	missense	probably damaging	1.00
IGL03036:Unc13b	APN	4	43,235,249 (GRCm39)	missense	probably damaging	1.00
IGL03209:Unc13b	APN	4	43,239,351 (GRCm39)	missense	probably damaging	0.99
IGL03352:Unc13b	APN	4	43,237,110 (GRCm39)	missense	possibly damaging	0.90
BB006:Unc13b	UTSW	4	43,174,399 (GRCm39)	missense	unknown
BB016:Unc13b	UTSW	4	43,174,399 (GRCm39)	missense	unknown
G1Funyon:Unc13b	UTSW	4	43,263,568 (GRCm39)	missense	probably benign
P0028:Unc13b	UTSW	4	43,256,225 (GRCm39)	missense	probably damaging	1.00
PIT4585001:Unc13b	UTSW	4	43,091,298 (GRCm39)	missense	probably benign	0.03
R0019:Unc13b	UTSW	4	43,096,990 (GRCm39)	missense	possibly damaging	0.58
R0019:Unc13b	UTSW	4	43,096,990 (GRCm39)	missense	possibly damaging	0.58
R0335:Unc13b	UTSW	4	43,236,983 (GRCm39)	missense	possibly damaging	0.95
R0504:Unc13b	UTSW	4	43,263,559 (GRCm39)	missense	probably damaging	0.99
R0631:Unc13b	UTSW	4	43,182,849 (GRCm39)	missense	possibly damaging	0.47
R0748:Unc13b	UTSW	4	43,241,164 (GRCm39)	splice site	probably benign
R1275:Unc13b	UTSW	4	43,235,366 (GRCm39)	missense	probably damaging	1.00
R1293:Unc13b	UTSW	4	43,235,190 (GRCm39)	missense	probably damaging	1.00
R1434:Unc13b	UTSW	4	43,239,385 (GRCm39)	nonsense	probably null
R1552:Unc13b	UTSW	4	43,237,144 (GRCm39)	missense	probably damaging	0.99
R1591:Unc13b	UTSW	4	43,244,747 (GRCm39)	missense	probably damaging	1.00
R1628:Unc13b	UTSW	4	43,263,371 (GRCm39)	missense	probably damaging	1.00
R1740:Unc13b	UTSW	4	43,240,285 (GRCm39)	missense	probably damaging	1.00
R1839:Unc13b	UTSW	4	43,258,308 (GRCm39)	splice site	probably benign
R2045:Unc13b	UTSW	4	43,091,266 (GRCm39)	missense	probably damaging	1.00
R2191:Unc13b	UTSW	4	43,245,566 (GRCm39)	nonsense	probably null
R2259:Unc13b	UTSW	4	43,182,780 (GRCm39)	missense	possibly damaging	0.87
R2307:Unc13b	UTSW	4	43,239,854 (GRCm39)	missense	probably damaging	0.98
R2317:Unc13b	UTSW	4	43,245,514 (GRCm39)	missense	probably damaging	1.00
R2402:Unc13b	UTSW	4	43,095,843 (GRCm39)	missense	probably benign
R2847:Unc13b	UTSW	4	43,180,404 (GRCm39)	missense	probably benign	0.04
R3414:Unc13b	UTSW	4	43,234,658 (GRCm39)	splice site	probably benign
R3436:Unc13b	UTSW	4	43,097,028 (GRCm39)	splice site	probably benign
R3955:Unc13b	UTSW	4	43,256,834 (GRCm39)	missense	probably damaging	1.00
R3957:Unc13b	UTSW	4	43,256,834 (GRCm39)	missense	probably damaging	1.00
R4015:Unc13b	UTSW	4	43,237,801 (GRCm39)	missense	probably damaging	1.00
R4650:Unc13b	UTSW	4	43,261,035 (GRCm39)	missense	probably damaging	0.97
R4836:Unc13b	UTSW	4	43,237,137 (GRCm39)	missense	probably damaging	1.00
R5041:Unc13b	UTSW	4	43,237,836 (GRCm39)	missense	probably benign	0.41
R5413:Unc13b	UTSW	4	43,257,936 (GRCm39)	critical splice donor site	probably null
R5994:Unc13b	UTSW	4	43,172,596 (GRCm39)	intron	probably benign
R6015:Unc13b	UTSW	4	43,177,995 (GRCm39)	nonsense	probably null
R6090:Unc13b	UTSW	4	43,239,306 (GRCm39)	missense	probably damaging	1.00
R6242:Unc13b	UTSW	4	43,165,800 (GRCm39)	missense	possibly damaging	0.92
R6246:Unc13b	UTSW	4	43,216,246 (GRCm39)	missense	probably benign	0.18
R6427:Unc13b	UTSW	4	43,176,966 (GRCm39)	unclassified	probably benign
R6660:Unc13b	UTSW	4	43,177,412 (GRCm39)	unclassified	probably benign
R6670:Unc13b	UTSW	4	43,255,562 (GRCm39)	missense	probably damaging	0.99
R6753:Unc13b	UTSW	4	43,239,331 (GRCm39)	missense	probably damaging	1.00
R6858:Unc13b	UTSW	4	43,165,828 (GRCm39)	missense	possibly damaging	0.85
R6886:Unc13b	UTSW	4	43,170,156 (GRCm39)	intron	probably benign
R6969:Unc13b	UTSW	4	43,263,538 (GRCm39)	missense	possibly damaging	0.94
R6994:Unc13b	UTSW	4	43,173,203 (GRCm39)	intron	probably benign
R6994:Unc13b	UTSW	4	43,171,403 (GRCm39)	intron	probably benign
R7080:Unc13b	UTSW	4	43,171,926 (GRCm39)	missense	unknown
R7117:Unc13b	UTSW	4	43,216,544 (GRCm39)	missense	probably benign	0.33
R7132:Unc13b	UTSW	4	43,215,757 (GRCm39)	missense	probably benign	0.17
R7181:Unc13b	UTSW	4	43,258,893 (GRCm39)	missense	probably damaging	0.99
R7192:Unc13b	UTSW	4	43,258,519 (GRCm39)	missense	probably damaging	1.00
R7246:Unc13b	UTSW	4	43,172,910 (GRCm39)	missense	unknown
R7342:Unc13b	UTSW	4	43,258,703 (GRCm39)	missense	probably damaging	0.99
R7345:Unc13b	UTSW	4	43,173,966 (GRCm39)	missense	unknown
R7355:Unc13b	UTSW	4	43,237,754 (GRCm39)	missense	probably damaging	1.00
R7391:Unc13b	UTSW	4	43,216,459 (GRCm39)	missense	probably benign	0.03
R7419:Unc13b	UTSW	4	43,174,023 (GRCm39)	missense	unknown
R7424:Unc13b	UTSW	4	43,172,235 (GRCm39)	missense	unknown
R7517:Unc13b	UTSW	4	43,215,765 (GRCm39)	missense	probably benign
R7532:Unc13b	UTSW	4	43,249,565 (GRCm39)	missense	probably benign	0.44
R7564:Unc13b	UTSW	4	43,091,258 (GRCm39)	missense	probably damaging	1.00
R7598:Unc13b	UTSW	4	43,263,569 (GRCm39)	missense	probably benign	0.20
R7604:Unc13b	UTSW	4	43,256,776 (GRCm39)	missense	possibly damaging	0.95
R7604:Unc13b	UTSW	4	43,170,102 (GRCm39)	missense	unknown
R7643:Unc13b	UTSW	4	43,216,333 (GRCm39)	missense	probably benign
R7718:Unc13b	UTSW	4	43,173,854 (GRCm39)	missense	unknown
R7735:Unc13b	UTSW	4	43,165,791 (GRCm39)	missense	probably damaging	1.00
R7756:Unc13b	UTSW	4	43,177,312 (GRCm39)	small deletion	probably benign
R7757:Unc13b	UTSW	4	43,177,341 (GRCm39)	small insertion	probably benign
R7757:Unc13b	UTSW	4	43,177,330 (GRCm39)	small insertion	probably benign
R7757:Unc13b	UTSW	4	43,177,312 (GRCm39)	small deletion	probably benign
R7758:Unc13b	UTSW	4	43,177,344 (GRCm39)	small insertion	probably benign
R7758:Unc13b	UTSW	4	43,177,312 (GRCm39)	small insertion	probably benign
R7781:Unc13b	UTSW	4	43,259,546 (GRCm39)	missense	possibly damaging	0.87
R7793:Unc13b	UTSW	4	43,172,737 (GRCm39)	missense	unknown
R7858:Unc13b	UTSW	4	43,176,285 (GRCm39)	missense	unknown
R7867:Unc13b	UTSW	4	43,232,573 (GRCm39)	nonsense	probably null
R7897:Unc13b	UTSW	4	43,171,860 (GRCm39)	missense	unknown
R7904:Unc13b	UTSW	4	43,217,075 (GRCm39)	missense	probably benign
R7929:Unc13b	UTSW	4	43,174,399 (GRCm39)	missense	unknown
R7984:Unc13b	UTSW	4	43,173,973 (GRCm39)	missense	unknown
R8069:Unc13b	UTSW	4	43,177,597 (GRCm39)	missense	unknown
R8101:Unc13b	UTSW	4	43,239,918 (GRCm39)	missense	probably benign	0.08
R8246:Unc13b	UTSW	4	43,175,954 (GRCm39)	missense	unknown
R8289:Unc13b	UTSW	4	43,172,524 (GRCm39)	nonsense	probably null
R8301:Unc13b	UTSW	4	43,263,568 (GRCm39)	missense	probably benign
R8397:Unc13b	UTSW	4	43,217,290 (GRCm39)	missense	probably benign	0.12
R8421:Unc13b	UTSW	4	43,178,304 (GRCm39)	missense	unknown
R8738:Unc13b	UTSW	4	43,177,564 (GRCm39)	missense	unknown
R8746:Unc13b	UTSW	4	43,176,120 (GRCm39)	missense	unknown
R8766:Unc13b	UTSW	4	43,174,722 (GRCm39)	missense	unknown
R8825:Unc13b	UTSW	4	43,237,683 (GRCm39)	splice site	probably benign
R8834:Unc13b	UTSW	4	43,175,954 (GRCm39)	missense	unknown
R8862:Unc13b	UTSW	4	43,235,207 (GRCm39)	missense	probably damaging	1.00
R8864:Unc13b	UTSW	4	43,174,724 (GRCm39)	missense	unknown
R8889:Unc13b	UTSW	4	43,176,484 (GRCm39)	missense	unknown
R8892:Unc13b	UTSW	4	43,176,484 (GRCm39)	missense	unknown
R8904:Unc13b	UTSW	4	43,178,531 (GRCm39)	intron	probably benign
R9089:Unc13b	UTSW	4	43,095,847 (GRCm39)	missense	probably damaging	1.00
R9144:Unc13b	UTSW	4	43,173,649 (GRCm39)	missense	unknown
R9149:Unc13b	UTSW	4	43,176,186 (GRCm39)	missense	unknown
R9173:Unc13b	UTSW	4	43,177,421 (GRCm39)	missense	unknown
R9200:Unc13b	UTSW	4	43,257,352 (GRCm39)	missense	possibly damaging	0.50
R9232:Unc13b	UTSW	4	43,240,321 (GRCm39)	missense	probably benign	0.03
R9269:Unc13b	UTSW	4	43,171,955 (GRCm39)	missense	unknown
R9320:Unc13b	UTSW	4	43,171,044 (GRCm39)	missense	unknown
R9335:Unc13b	UTSW	4	43,255,551 (GRCm39)	missense	probably damaging	0.99
R9335:Unc13b	UTSW	4	43,216,123 (GRCm39)	missense	possibly damaging	0.86
R9352:Unc13b	UTSW	4	43,177,312 (GRCm39)	small insertion	probably benign
R9378:Unc13b	UTSW	4	43,173,282 (GRCm39)	missense	unknown
R9382:Unc13b	UTSW	4	43,172,512 (GRCm39)	missense	unknown
R9569:Unc13b	UTSW	4	43,177,312 (GRCm39)	small deletion	probably benign
R9622:Unc13b	UTSW	4	43,172,513 (GRCm39)	missense
R9687:Unc13b	UTSW	4	43,174,920 (GRCm39)	missense	unknown
R9704:Unc13b	UTSW	4	43,237,102 (GRCm39)	missense	probably benign	0.31
R9721:Unc13b	UTSW	4	43,101,869 (GRCm39)	missense	probably benign
R9753:Unc13b	UTSW	4	43,182,842 (GRCm39)	nonsense	probably null
RF016:Unc13b	UTSW	4	43,177,350 (GRCm39)	small insertion	probably benign
RF016:Unc13b	UTSW	4	43,177,347 (GRCm39)	small insertion	probably benign
RF041:Unc13b	UTSW	4	43,177,338 (GRCm39)	small insertion	probably benign
RF056:Unc13b	UTSW	4	43,177,359 (GRCm39)	small insertion	probably benign
Z1176:Unc13b	UTSW	4	43,177,764 (GRCm39)	missense	unknown
Z1176:Unc13b	UTSW	4	43,177,191 (GRCm39)	missense	unknown
Z1176:Unc13b	UTSW	4	43,171,419 (GRCm39)	missense	unknown
Z1176:Unc13b	UTSW	4	43,261,043 (GRCm39)	missense	probably benign	0.11
Z1177:Unc13b	UTSW	4	43,173,669 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CCCCATGTTTTGAAGTACTTGC -3'
(R):5'- GCTCCTGCCTCATCAAACAG -3'

Sequencing Primer
(F):5'- CTGTCTCAGAGGATGATATGGGAC -3'
(R):5'- TGCCTCATCAAACAGTGAAATACTAG -3'

Posted On

2022-04-18