Mutagenetix > Incidental Mutation

Incidental Mutation 'R9354:Gpr146'

708259

Institutional Source

Beutler Lab

Gene Symbol

Gpr146

Ensembl Gene

ENSMUSG00000044197

Gene Name

G protein-coupled receptor 146

Synonyms

PGR8

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9354 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

139363452-139382170 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 139378366 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 56 (N56S)

Ref Sequence

ENSEMBL: ENSMUSP00000049707 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051293] [ENSMUST00000066052] [ENSMUST00000100514] [ENSMUST00000138631] [ENSMUST00000198474]

AlphaFold

Q99LE2

Predicted Effect

probably benign
Transcript: ENSMUST00000051293
AA Change: N56S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000049707
Gene: ENSMUSG00000044197
AA Change: N56S

Domain	Start	End	E-Value	Type
low complexity region	21	36	N/A	INTRINSIC
Pfam:7tm_1	43	294	2e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000066052

SMART Domains

Protein: ENSMUSP00000069230
Gene: ENSMUSG00000053553

Domain	Start	End	E-Value	Type
low complexity region	54	77	N/A	INTRINSIC
Pfam:DUF2373	103	165	3e-26	PFAM
low complexity region	184	194	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100514
AA Change: N56S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000098083
Gene: ENSMUSG00000044197
AA Change: N56S

Domain	Start	End	E-Value	Type
low complexity region	21	36	N/A	INTRINSIC
Pfam:7tm_1	43	294	1.6e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000138631
AA Change: N56S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000119464
Gene: ENSMUSG00000044197
AA Change: N56S

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	26	80	3e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000198474

SMART Domains

Protein: ENSMUSP00000142949
Gene: ENSMUSG00000053553

Domain	Start	End	E-Value	Type
low complexity region	54	77	N/A	INTRINSIC
Pfam:DUF2373	102	141	9e-12	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933409G03Rik	A	G	2: 68,436,873 (GRCm39)	Q181R	unknown	Het
Acsl1	T	G	8: 46,966,753 (GRCm39)	C242G	probably benign	Het
Agap2	A	C	10: 126,923,104 (GRCm39)	N646T	unknown	Het
Ankrd44	A	T	1: 54,687,438 (GRCm39)	*994R	probably null	Het
Apol7c	C	T	15: 77,410,112 (GRCm39)	R278H	possibly damaging	Het
Birc6	A	G	17: 74,921,401 (GRCm39)	E2166G	probably benign	Het
Caskin2	G	A	11: 115,693,468 (GRCm39)	T528M	probably damaging	Het
Ccdc30	G	T	4: 119,230,850 (GRCm39)	P15Q	possibly damaging	Het
Cemip2	A	G	19: 21,779,389 (GRCm39)	S400G	probably benign	Het
Cenpf	G	A	1: 189,379,114 (GRCm39)	T45I		Het
Cep68	T	C	11: 20,188,569 (GRCm39)	E612G	probably damaging	Het
Chpf2	T	C	5: 24,796,392 (GRCm39)	L446P	probably damaging	Het
Ctsl	A	T	13: 64,516,850 (GRCm39)	Y40N	probably damaging	Het
Dis3l	T	C	9: 64,221,922 (GRCm39)	N496S	probably benign	Het
Espnl	A	T	1: 91,272,323 (GRCm39)	D561V	probably benign	Het
Flnb	T	A	14: 7,818,411 (GRCm38)	L87Q	probably benign	Het
Gbp9	T	A	5: 105,232,825 (GRCm39)	I276F	possibly damaging	Het
Gid4	G	T	11: 60,308,618 (GRCm39)	R46L	probably benign	Het
Gper1	G	A	5: 139,412,029 (GRCm39)	D125N	possibly damaging	Het
Grhpr	G	T	4: 44,981,465 (GRCm39)	R5L	probably benign	Het
Gstm6	A	T	3: 107,850,018 (GRCm39)	N59K	probably damaging	Het
Hdac7	A	T	15: 97,694,769 (GRCm39)	F802L	probably damaging	Het
Igfbpl1	A	T	4: 45,816,348 (GRCm39)	V159D	probably damaging	Het
Itga8	T	C	2: 12,237,668 (GRCm39)	S351G	possibly damaging	Het
Itgad	C	A	7: 127,785,146 (GRCm39)	H354Q	probably damaging	Het
Jmjd1c	T	C	10: 67,059,875 (GRCm39)	S641P	probably damaging	Het
Klhl33	T	C	14: 51,130,385 (GRCm39)	T110A	probably benign	Het
Lcorl	T	A	5: 45,890,968 (GRCm39)	K545*	probably null	Het
Lilra6	A	G	7: 3,914,628 (GRCm39)	S546P	probably damaging	Het
Ly6c1	T	A	15: 74,916,471 (GRCm39)	I124F	probably benign	Het
Map4	A	G	9: 109,897,847 (GRCm39)	T858A	probably benign	Het
Marchf5	G	T	19: 37,185,264 (GRCm39)		probably benign	Het
Muc2	A	G	7: 141,307,157 (GRCm39)	K769E		Het
Myh6	T	A	14: 55,200,992 (GRCm39)	I157F	probably damaging	Het
Naip1	A	G	13: 100,563,994 (GRCm39)	V389A	probably benign	Het
Or52b3	G	A	7: 102,204,397 (GRCm39)	R302H	possibly damaging	Het
Pcdhgc4	A	G	18: 37,949,640 (GRCm39)	H352R	probably benign	Het
Pde8a	C	T	7: 80,982,619 (GRCm39)	T746I	probably damaging	Het
Pick1	T	C	15: 79,123,848 (GRCm39)	V73A	probably damaging	Het
Ppwd1	C	T	13: 104,342,080 (GRCm39)	V625I	probably benign	Het
Prss59	A	G	6: 40,905,473 (GRCm39)	V61A	probably damaging	Het
Prune2	A	G	19: 17,099,986 (GRCm39)	E1830G	probably benign	Het
Ralgapb	A	G	2: 158,279,313 (GRCm39)	N445S	possibly damaging	Het
Rapgef6	G	T	11: 54,510,749 (GRCm39)	R222L	possibly damaging	Het
Rorc	A	T	3: 94,280,170 (GRCm39)		probably benign	Het
Rps6ka1	A	C	4: 133,594,432 (GRCm39)		probably null	Het
Sdk2	T	C	11: 113,725,757 (GRCm39)	Y1164C	probably benign	Het
Sema5a	T	A	15: 32,562,902 (GRCm39)	Y304*	probably null	Het
Slc26a4	A	T	12: 31,585,255 (GRCm39)	V513D	possibly damaging	Het
Slf2	T	A	19: 44,936,471 (GRCm39)	D705E	probably damaging	Het
Spag17	A	G	3: 99,934,905 (GRCm39)	T704A	probably benign	Het
Tex15	C	G	8: 34,063,344 (GRCm39)	Q925E	possibly damaging	Het
Trim38	A	T	13: 23,969,875 (GRCm39)	T145S	probably benign	Het
Trpm3	G	A	19: 22,425,696 (GRCm39)	C17Y	probably benign	Het
Usp42	A	G	5: 143,701,027 (GRCm39)	Y999H	probably benign	Het
Vat1	A	C	11: 101,351,441 (GRCm39)	M300R	probably benign	Het
Vmn1r85	T	A	7: 12,818,725 (GRCm39)	I140F	probably damaging	Het
Vmn2r57	T	C	7: 41,049,663 (GRCm39)	I695M	probably benign	Het
Wnk1	C	T	6: 119,942,660 (GRCm39)	R789Q	unknown	Het
Zswim5	G	A	4: 116,844,232 (GRCm39)	G1090D	probably damaging	Het

Other mutations in Gpr146

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01355:Gpr146	APN	5	139,364,659 (GRCm39)	intron	probably benign
IGL02152:Gpr146	APN	5	139,378,467 (GRCm39)	missense	probably damaging	1.00
IGL02369:Gpr146	APN	5	139,378,443 (GRCm39)	missense	probably benign	0.00
IGL02392:Gpr146	APN	5	139,378,533 (GRCm39)	missense	probably damaging	0.99
IGL02828:Gpr146	APN	5	139,378,576 (GRCm39)	missense	probably damaging	0.97
IGL03095:Gpr146	APN	5	139,378,705 (GRCm39)	missense	probably benign	0.11
R0360:Gpr146	UTSW	5	139,364,933 (GRCm39)	intron	probably benign
R0364:Gpr146	UTSW	5	139,364,933 (GRCm39)	intron	probably benign
R0746:Gpr146	UTSW	5	139,378,977 (GRCm39)	missense	probably damaging	1.00
R1446:Gpr146	UTSW	5	139,379,177 (GRCm39)	missense	probably benign	0.00
R1507:Gpr146	UTSW	5	139,379,124 (GRCm39)	missense	probably benign
R1758:Gpr146	UTSW	5	139,379,137 (GRCm39)	missense	probably benign	0.34
R2032:Gpr146	UTSW	5	139,364,902 (GRCm39)	intron	probably benign
R6513:Gpr146	UTSW	5	139,378,573 (GRCm39)	missense	probably damaging	1.00
R6797:Gpr146	UTSW	5	139,378,795 (GRCm39)	missense	possibly damaging	0.79
R7830:Gpr146	UTSW	5	139,378,357 (GRCm39)	missense	probably benign	0.02
R7977:Gpr146	UTSW	5	139,378,440 (GRCm39)	missense	possibly damaging	0.53
R7987:Gpr146	UTSW	5	139,378,440 (GRCm39)	missense	possibly damaging	0.53
R8225:Gpr146	UTSW	5	139,378,371 (GRCm39)	missense	probably benign	0.03
R8792:Gpr146	UTSW	5	139,378,549 (GRCm39)	missense	probably damaging	1.00
X0064:Gpr146	UTSW	5	139,364,664 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- GGACAGTGGTGACTAACTCTGTC -3'
(R):5'- TACATGGTCACCAGGGAAGC -3'

Sequencing Primer
(F):5'- TCTCTCTCAACAGCTGCGAAG -3'
(R):5'- CCACGTTGAACAGGATGAGC -3'

Posted On

2022-04-18