Mutagenetix > Incidental Mutation

Incidental Mutation 'R9360:Taar7e'

708637

Institutional Source

Beutler Lab

Gene Symbol

Taar7e

Ensembl Gene

ENSMUSG00000100689

Gene Name

trace amine-associated receptor 7E

Synonyms

LOC276742

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.139) question?

Stock #

R9360 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23913512-23914588 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 23913949 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 146 (Y146*)

Ref Sequence

ENSEMBL: ENSMUSP00000090326 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092656]

AlphaFold

Q5QD09

Predicted Effect

probably null
Transcript: ENSMUST00000092656
AA Change: Y146*

SMART Domains

Protein: ENSMUSP00000090326
Gene: ENSMUSG00000100689
AA Change: Y146*

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	56	261	5.4e-8	PFAM
Pfam:7TM_GPCR_Srsx	58	341	4.3e-10	PFAM
Pfam:7tm_1	64	326	9.4e-59	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam4	A	G	12: 81,468,261 (GRCm39)	L120P	probably damaging	Het
Asb6	A	G	2: 30,714,334 (GRCm39)	S259P	probably damaging	Het
Atp6v0a2	C	A	5: 124,767,259 (GRCm39)	S7R	possibly damaging	Het
Btbd16	C	T	7: 130,417,516 (GRCm39)	R344C	probably damaging	Het
Cacna1h	G	A	17: 25,594,336 (GRCm39)	A2274V	probably benign	Het
Caml	A	C	13: 55,771,030 (GRCm39)	N43H	probably damaging	Het
Ccar2	A	G	14: 70,379,445 (GRCm39)	S518P	probably damaging	Het
Cdkl3	A	G	11: 51,924,349 (GRCm39)	I546V	probably null	Het
Chac1	C	T	2: 119,182,854 (GRCm39)	T84I	probably damaging	Het
Col6a6	T	C	9: 105,644,686 (GRCm39)	T1201A	probably benign	Het
Csf1	T	C	3: 107,661,158 (GRCm39)	T120A	possibly damaging	Het
Ctsj	T	A	13: 61,151,634 (GRCm39)	I95F	probably benign	Het
Cyp4f17	C	T	17: 32,743,880 (GRCm39)	R353C	probably benign	Het
Depdc1a	A	G	3: 159,232,168 (GRCm39)	M640V	possibly damaging	Het
Dgkq	A	G	5: 108,798,469 (GRCm39)	V633A	probably damaging	Het
Fdft1	A	T	14: 63,415,189 (GRCm39)	Y14*	probably null	Het
Fktn	G	A	4: 53,734,854 (GRCm39)	G125D	probably benign	Het
Ftdc1	T	C	16: 58,434,234 (GRCm39)	D161G	probably benign	Het
Gfm2	T	A	13: 97,289,752 (GRCm39)	V179E	probably damaging	Het
Gpr12	A	G	5: 146,520,299 (GRCm39)	F208L	probably benign	Het
Greb1	A	G	12: 16,790,037 (GRCm39)	S4P	probably damaging	Het
Hlcs	A	T	16: 93,932,672 (GRCm39)	I717N	probably damaging	Het
Hormad1	G	A	3: 95,483,622 (GRCm39)	A145T	probably benign	Het
Kcmf1	A	T	6: 72,838,826 (GRCm39)	C33*	probably null	Het
Kif13a	A	T	13: 46,962,472 (GRCm39)	S561T	probably benign	Het
Klhl20	G	T	1: 160,921,269 (GRCm39)	P571Q	probably benign	Het
Kpna6	A	T	4: 129,547,635 (GRCm39)	M304K	probably benign	Het
Krt75	T	C	15: 101,476,729 (GRCm39)	K387E	probably damaging	Het
Lum	A	T	10: 97,404,752 (GRCm39)	K216*	probably null	Het
Meioc	A	G	11: 102,565,779 (GRCm39)	N409S	probably benign	Het
Mrpl20	T	C	4: 155,888,402 (GRCm39)		probably null	Het
Naa16	A	T	14: 79,593,943 (GRCm39)	I374N	probably benign	Het
Nbea	A	G	3: 55,943,319 (GRCm39)	I652T	possibly damaging	Het
Nhsl1	G	A	10: 18,194,898 (GRCm39)	G56R	probably damaging	Het
Obscn	T	C	11: 58,973,650 (GRCm39)	I1894V	probably benign	Het
Olig2	A	C	16: 91,023,774 (GRCm39)	T163P	probably damaging	Het
Or13c7c	A	G	4: 43,835,765 (GRCm39)	S242P	probably benign	Het
Or4g7	T	C	2: 111,309,684 (GRCm39)	I185T	probably benign	Het
Or5p81	T	A	7: 108,266,977 (GRCm39)	L118Q	probably damaging	Het
Or7e168	T	A	9: 19,720,529 (GRCm39)	V305D	possibly damaging	Het
Parp4	G	A	14: 56,878,775 (GRCm39)		probably null	Het
Pcdhb2	T	C	18: 37,429,551 (GRCm39)	I508T	probably damaging	Het
Pcdhga8	T	C	18: 37,859,787 (GRCm39)	F281S	probably damaging	Het
Pom121l12	T	A	11: 14,549,516 (GRCm39)	M74K	possibly damaging	Het
Pramel39-ps	G	T	5: 94,451,001 (GRCm39)	P375H	probably damaging	Het
Prpf8	T	C	11: 75,381,156 (GRCm39)	Y219H	possibly damaging	Het
Rhag	T	C	17: 41,142,548 (GRCm39)	V251A	possibly damaging	Het
Rrs1	A	G	1: 9,616,845 (GRCm39)	*366W	probably null	Het
Sgpp2	A	G	1: 78,367,143 (GRCm39)	D92G	probably damaging	Het
Slc44a3	C	A	3: 121,325,908 (GRCm39)		probably benign	Het
Smg8	T	C	11: 86,968,956 (GRCm39)	M257V	probably benign	Het
Tet2	G	T	3: 133,192,903 (GRCm39)	N510K	possibly damaging	Het
Tgfbr3	T	C	5: 107,257,550 (GRCm39)	I836M	unknown	Het
Thada	A	G	17: 84,499,410 (GRCm39)	V1929A	probably benign	Het
Tram1l1	A	T	3: 124,115,899 (GRCm39)	D353V	probably damaging	Het
Trf	T	A	9: 103,094,734 (GRCm39)		probably null	Het
Trim15	A	T	17: 37,177,942 (GRCm39)	C18S	probably damaging	Het
Vmn1r62	T	C	7: 5,678,952 (GRCm39)	I211T	probably damaging	Het
Zdhhc17	A	G	10: 110,783,165 (GRCm39)	V501A	probably benign	Het
Zfp563	A	G	17: 33,324,428 (GRCm39)	E341G	probably benign	Het

Other mutations in Taar7e

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00494:Taar7e	APN	10	23,914,038 (GRCm39)	missense	probably benign
IGL03150:Taar7e	APN	10	23,913,528 (GRCm39)	missense	probably benign	0.01
IGL03366:Taar7e	APN	10	23,913,813 (GRCm39)	missense	probably damaging	1.00
R2013:Taar7e	UTSW	10	23,913,732 (GRCm39)	missense	possibly damaging	0.63
R2095:Taar7e	UTSW	10	23,913,949 (GRCm39)	nonsense	probably null
R4210:Taar7e	UTSW	10	23,913,932 (GRCm39)	missense	probably damaging	1.00
R4211:Taar7e	UTSW	10	23,913,932 (GRCm39)	missense	probably damaging	1.00
R4766:Taar7e	UTSW	10	23,914,464 (GRCm39)	missense	probably damaging	0.99
R6085:Taar7e	UTSW	10	23,913,761 (GRCm39)	missense	probably benign	0.03
R6117:Taar7e	UTSW	10	23,914,427 (GRCm39)	missense	probably damaging	0.98
R6918:Taar7e	UTSW	10	23,913,513 (GRCm39)	start codon destroyed	probably null	0.15
R7410:Taar7e	UTSW	10	23,914,424 (GRCm39)	missense	probably benign
R7913:Taar7e	UTSW	10	23,913,902 (GRCm39)	missense	possibly damaging	0.79
R9008:Taar7e	UTSW	10	23,913,810 (GRCm39)	missense	probably damaging	1.00
R9081:Taar7e	UTSW	10	23,913,893 (GRCm39)	missense	probably benign	0.00
R9205:Taar7e	UTSW	10	23,913,972 (GRCm39)	missense	probably benign
R9465:Taar7e	UTSW	10	23,914,310 (GRCm39)	missense	possibly damaging	0.81
R9790:Taar7e	UTSW	10	23,913,554 (GRCm39)	missense	probably benign	0.00
R9791:Taar7e	UTSW	10	23,913,554 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TTTCAGGCAGCTGCACTCTC -3'
(R):5'- CACTGCAAGTTGACAGCCAC -3'

Sequencing Primer
(F):5'- AACTTCCTGGTGGCATCCCTG -3'
(R):5'- GTTGACAGCCACCCACACAG -3'

Posted On

2022-04-18