Incidental Mutation 'R9364:Mettl15'
ID 708820
Institutional Source Beutler Lab
Gene Symbol Mettl15
Ensembl Gene ENSMUSG00000057234
Gene Name methyltransferase like 15
Synonyms 0610027B03Rik, Mett5d1
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.126) question?
Stock # R9364 (G1)
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 108922642-109111093 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 108961960 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamine to Histidine at position 216 (Q216H)
Ref Sequence ENSEMBL: ENSMUSP00000080337 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000081631] [ENSMUST00000147770]
AlphaFold Q9DCL4
Predicted Effect probably benign
Transcript: ENSMUST00000081631
AA Change: Q216H

PolyPhen 2 Score 0.059 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000080337
Gene: ENSMUSG00000057234
AA Change: Q216H

DomainStartEndE-ValueType
Pfam:Methyltransf_5 69 406 1.5e-90 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000147770
AA Change: Q216H

PolyPhen 2 Score 0.059 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000116829
Gene: ENSMUSG00000057234
AA Change: Q216H

DomainStartEndE-ValueType
Pfam:Methyltransf_5 69 230 1.6e-47 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 62 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2200002D01Rik CCTTCTCCTTCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC CCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC 7: 28,947,048 (GRCm39) probably benign Het
Ankrd17 C T 5: 90,416,508 (GRCm39) R1108Q probably damaging Het
Ccdc136 C T 6: 29,405,960 (GRCm39) A102V probably damaging Het
Ccn4 G A 15: 66,784,900 (GRCm39) R191K probably benign Het
Ccnyl1 A G 1: 64,753,750 (GRCm39) Y187C probably damaging Het
Copa G A 1: 171,944,831 (GRCm39) V882I probably benign Het
Cyp17a1 C T 19: 46,657,165 (GRCm39) R361H probably damaging Het
Ddx49 T C 8: 70,746,226 (GRCm39) T459A probably benign Het
Depdc5 A G 5: 33,122,076 (GRCm39) N1133S probably benign Het
Dnajc18 T C 18: 35,808,260 (GRCm39) D329G probably damaging Het
Dusp16 G A 6: 134,695,982 (GRCm39) P283L probably damaging Het
Efcab12 A G 6: 115,814,975 (GRCm39) V40A probably benign Het
Emb G T 13: 117,357,096 (GRCm39) probably benign Het
Fam120b G A 17: 15,626,020 (GRCm39) A458T possibly damaging Het
Fgd4 G A 16: 16,308,353 (GRCm39) T9I probably benign Het
Hivep1 G A 13: 42,308,251 (GRCm39) D164N possibly damaging Het
Klf13 T A 7: 63,574,609 (GRCm39) probably benign Het
Lrrc47 T C 4: 154,100,398 (GRCm39) S325P possibly damaging Het
Mast3 C T 8: 71,238,826 (GRCm39) V493M probably damaging Het
Med22 T C 2: 26,795,821 (GRCm39) T200A probably benign Het
Mmel1 A G 4: 154,976,967 (GRCm39) D561G probably null Het
Ms4a4a G A 19: 11,367,708 (GRCm39) M191I probably benign Het
Mtcl3 G A 10: 29,072,775 (GRCm39) R689Q probably damaging Het
Myh11 T A 16: 14,018,580 (GRCm39) N1922I Het
Myo7b T C 18: 32,133,413 (GRCm39) I371V probably benign Het
Myo9b T C 8: 71,808,483 (GRCm39) S1697P probably damaging Het
Neurod4 T C 10: 130,106,840 (GRCm39) T145A probably benign Het
Nfx1 T C 4: 41,023,756 (GRCm39) V1055A probably benign Het
Ntng1 C T 3: 110,042,680 (GRCm39) A49T probably damaging Het
Oc90 G A 15: 65,761,437 (GRCm39) P194S probably benign Het
Opcml A C 9: 28,814,624 (GRCm39) N292T probably damaging Het
Oplah A G 15: 76,193,787 (GRCm39) S57P probably benign Het
Or1x6 T A 11: 50,939,223 (GRCm39) Y96* probably null Het
Or2a12 T A 6: 42,904,534 (GRCm39) I123N probably damaging Het
Or2aj4 T A 16: 19,384,722 (GRCm39) I304F possibly damaging Het
Or8k3b A T 2: 86,520,575 (GRCm39) V248D possibly damaging Het
Pdzd8 A T 19: 59,333,574 (GRCm39) L149Q probably damaging Het
Pid1 A G 1: 84,137,032 (GRCm39) V33A probably benign Het
Prpsap1 A G 11: 116,385,015 (GRCm39) probably benign Het
R3hdml T C 2: 163,334,535 (GRCm39) W42R probably benign Het
Rnase6 A T 14: 51,367,862 (GRCm39) N85Y possibly damaging Het
Scn3a T A 2: 65,291,596 (GRCm39) M1717L possibly damaging Het
Setbp1 C T 18: 78,826,599 (GRCm39) S1338N probably benign Het
Sh2b2 T C 5: 136,253,006 (GRCm39) T389A probably benign Het
Sh2d4a G A 8: 68,747,018 (GRCm39) G82D probably damaging Het
Skint9 A T 4: 112,248,915 (GRCm39) M171K probably benign Het
Slc5a11 C T 7: 122,868,324 (GRCm39) R505W probably damaging Het
Slx4 C A 16: 3,805,820 (GRCm39) M577I probably benign Het
Tarbp1 C T 8: 127,177,462 (GRCm39) V737I probably benign Het
Tm7sf3 C T 6: 146,525,179 (GRCm39) D89N possibly damaging Het
Tmem100 A T 11: 89,926,533 (GRCm39) E120V probably damaging Het
Trim72 C T 7: 127,609,173 (GRCm39) T325M possibly damaging Het
Trpc2 G A 7: 101,739,819 (GRCm39) G581D possibly damaging Het
Tyw1 G A 5: 130,298,065 (GRCm39) R202Q probably damaging Het
Ubn1 T C 16: 4,888,492 (GRCm39) S154P unknown Het
Uchl1 T A 5: 66,833,649 (GRCm39) M6K probably damaging Het
Vmn1r193 A T 13: 22,403,989 (GRCm39) M1K probably null Het
Zfp429 T A 13: 67,538,531 (GRCm39) K304N probably benign Het
Zfp618 A G 4: 63,036,824 (GRCm39) N375D probably damaging Het
Zfp7 TGCGGGAAAGGTTTCCACCTGAGCG TGCG 15: 76,774,800 (GRCm39) probably benign Het
Zfp78 A T 7: 6,382,354 (GRCm39) D468V probably benign Het
Zfp809 A C 9: 22,150,394 (GRCm39) Y297S probably damaging Het
Other mutations in Mettl15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00155:Mettl15 APN 2 108,923,521 (GRCm39) nonsense probably null
IGL00657:Mettl15 APN 2 108,923,552 (GRCm39) missense probably damaging 1.00
IGL02684:Mettl15 APN 2 108,961,925 (GRCm39) missense probably damaging 0.97
IGL03367:Mettl15 APN 2 108,961,916 (GRCm39) missense probably benign 0.10
R1433:Mettl15 UTSW 2 108,923,266 (GRCm39) missense probably benign 0.00
R1538:Mettl15 UTSW 2 108,962,010 (GRCm39) critical splice acceptor site probably null
R3890:Mettl15 UTSW 2 109,021,924 (GRCm39) missense probably benign 0.03
R5464:Mettl15 UTSW 2 109,021,967 (GRCm39) missense probably benign 0.00
R6609:Mettl15 UTSW 2 108,967,687 (GRCm39) missense probably null 1.00
R7619:Mettl15 UTSW 2 108,923,220 (GRCm39) nonsense probably null
R7737:Mettl15 UTSW 2 108,967,723 (GRCm39) missense probably damaging 0.99
R8914:Mettl15 UTSW 2 108,967,625 (GRCm39) intron probably benign
R9121:Mettl15 UTSW 2 109,104,948 (GRCm39) missense
R9159:Mettl15 UTSW 2 108,923,444 (GRCm39) missense probably damaging 1.00
R9192:Mettl15 UTSW 2 109,104,810 (GRCm39) nonsense probably null
R9566:Mettl15 UTSW 2 108,923,592 (GRCm39) missense possibly damaging 0.61
Predicted Primers PCR Primer
(F):5'- TTAGTTGAACTTGTTACTCTTAGACAG -3'
(R):5'- AGACTGAGGCAAGTTAAAGTCAA -3'

Sequencing Primer
(F):5'- ACTTGTTACTCTTAGACAGTACAGAG -3'
(R):5'- CAATGTGTGAAGATCTCTACCAATG -3'
Posted On 2022-04-18