Mutagenetix > Incidental Mutation

Incidental Mutation 'R9373:Or8b9'

709443

Institutional Source

Beutler Lab

Gene Symbol

Or8b9

Ensembl Gene

ENSMUSG00000066749

Gene Name

olfactory receptor family 8 subfamily B member 9

Synonyms

Olfr877, MOR161-5, GA_x6K02T2PVTD-31540342-31541277

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R9373 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

37766116-37767051 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 37766750 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 212 (V212D)

Ref Sequence

ENSEMBL: ENSMUSP00000150698 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086063] [ENSMUST00000213956]

AlphaFold

Q8VF62

Predicted Effect

probably damaging
Transcript: ENSMUST00000086063
AA Change: V212D

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000083230
Gene: ENSMUSG00000066749
AA Change: V212D

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.4e-49	PFAM
Pfam:7tm_1	41	291	6.5e-20	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213956
AA Change: V212D

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

96% (53/55)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2200002D01Rik	CCTTCTCCTTCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC	CCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC	7: 28,947,048 (GRCm39)		probably benign	Het
2310003L06Rik	A	C	5: 88,120,668 (GRCm39)	D475A	probably benign	Het
Aatk	T	C	11: 119,906,343 (GRCm39)	M243V	possibly damaging	Het
Acox1	A	T	11: 116,065,173 (GRCm39)	N627K	possibly damaging	Het
Adal	A	G	2: 120,980,703 (GRCm39)	Y142C	probably benign	Het
Agfg2	A	G	5: 137,662,476 (GRCm39)		probably null	Het
Ajap1	C	T	4: 153,516,670 (GRCm39)	A224T	probably benign	Het
Alg1	T	C	16: 5,056,990 (GRCm39)	F234L	probably benign	Het
Arhgap27	G	T	11: 103,251,287 (GRCm39)	A147E	possibly damaging	Het
C9orf72	A	G	4: 35,196,985 (GRCm39)	F360S		Het
Cachd1	T	C	4: 100,832,067 (GRCm39)	V743A	possibly damaging	Het
Cc2d2b	G	A	19: 40,784,167 (GRCm39)	V655I	unknown	Het
Cd72	T	C	4: 43,450,141 (GRCm39)	S256G	possibly damaging	Het
Celf2	G	T	2: 6,551,915 (GRCm39)	N521K	probably benign	Het
Clca4a	T	A	3: 144,672,133 (GRCm39)	N270Y	possibly damaging	Het
Clvs2	A	T	10: 33,404,382 (GRCm39)	V278E	probably benign	Het
Cwf19l2	T	C	9: 3,454,718 (GRCm39)	F677S	probably damaging	Het
Dnaaf4	A	G	9: 72,871,462 (GRCm39)	T241A	probably damaging	Het
Dusp9	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	X: 72,684,217 (GRCm39)		probably benign	Het
Epdr1	T	C	13: 19,778,707 (GRCm39)	N130D	possibly damaging	Het
Fam184a	G	A	10: 53,566,115 (GRCm39)	R491W	probably benign	Het
Foxp2	A	G	6: 15,377,969 (GRCm39)	Q160R	unknown	Het
Fstl5	T	A	3: 76,555,669 (GRCm39)	Y515*	probably null	Het
Garin5b	T	C	7: 4,760,712 (GRCm39)	M667V		Het
Helz2	G	T	2: 180,882,741 (GRCm39)	N17K	probably benign	Het
Irag1	A	G	7: 110,545,038 (GRCm39)		probably null	Het
Jmjd1c	C	T	10: 66,932,495 (GRCm39)		probably benign	Het
Jup	C	T	11: 100,270,391 (GRCm39)	C372Y	probably damaging	Het
Lrrn1	A	C	6: 107,545,465 (GRCm39)	Q421P	possibly damaging	Het
Mapk1	A	G	16: 16,836,154 (GRCm39)	I101V	probably benign	Het
Me2	T	C	18: 73,918,800 (GRCm39)	E427G	probably damaging	Het
Mrc1	G	A	2: 14,274,999 (GRCm39)	M433I	probably damaging	Het
Nelfb	G	A	2: 25,095,218 (GRCm39)	R324W	probably damaging	Het
Nlrp4b	T	A	7: 10,449,126 (GRCm39)	I443K	probably benign	Het
Nup210l	C	T	3: 90,107,173 (GRCm39)	P1570L	probably benign	Het
Or5b118	A	G	19: 13,449,216 (GRCm39)	E294G	probably damaging	Het
Or8b50	G	A	9: 38,518,142 (GRCm39)	C127Y	possibly damaging	Het
Or9g3	A	T	2: 85,590,275 (GRCm39)	Y148*	probably null	Het
Parvb	C	T	15: 84,188,100 (GRCm39)	T281I	probably damaging	Het
Prune2	A	G	19: 17,099,502 (GRCm39)	T1669A	probably benign	Het
Runx3	A	G	4: 134,848,456 (GRCm39)	T14A	probably benign	Het
Scn9a	A	G	2: 66,314,261 (GRCm39)	V1819A	probably benign	Het
Senp1	T	C	15: 97,964,435 (GRCm39)	T260A	probably benign	Het
Serpinb10	A	G	1: 107,474,749 (GRCm39)	R304G	possibly damaging	Het
Spem1	C	T	11: 69,712,640 (GRCm39)	C65Y	probably benign	Het
Tdrd6	A	G	17: 43,939,053 (GRCm39)	V665A	possibly damaging	Het
Tenm2	C	T	11: 35,930,713 (GRCm39)	A1772T	probably damaging	Het
Tenm3	C	T	8: 48,752,690 (GRCm39)	V891I	probably damaging	Het
Trank1	A	G	9: 111,194,259 (GRCm39)	D761G	probably benign	Het
Tut7	T	C	13: 59,944,681 (GRCm39)	S1053G	probably damaging	Het
Ubap2l	A	T	3: 89,915,587 (GRCm39)	Y985N	unknown	Het
Vwc2l	A	T	1: 70,768,218 (GRCm39)	H94L	probably damaging	Het
Wdr27	G	A	17: 15,154,795 (GRCm39)	H41Y	probably benign	Het
Zfp7	AGTGCGGGAAAGGTTTCCACCTG	AG	15: 76,774,798 (GRCm39)		probably benign	Het
Zfp7	TGCGGGAAAGGTTTCCACCTGAGCG	TGCG	15: 76,774,800 (GRCm39)		probably benign	Het

Other mutations in Or8b9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01862:Or8b9	APN	9	37,766,477 (GRCm39)	missense	probably damaging	1.00
IGL02108:Or8b9	APN	9	37,766,234 (GRCm39)	missense	possibly damaging	0.88
IGL02474:Or8b9	APN	9	37,766,656 (GRCm39)	missense	probably benign	0.08
R0006:Or8b9	UTSW	9	37,766,516 (GRCm39)	missense	possibly damaging	0.95
R0893:Or8b9	UTSW	9	37,766,492 (GRCm39)	missense	probably damaging	1.00
R1051:Or8b9	UTSW	9	37,766,657 (GRCm39)	missense	probably damaging	0.99
R1432:Or8b9	UTSW	9	37,766,548 (GRCm39)	missense	possibly damaging	0.79
R1718:Or8b9	UTSW	9	37,766,749 (GRCm39)	missense	probably benign	0.03
R1864:Or8b9	UTSW	9	37,766,560 (GRCm39)	missense	probably damaging	1.00
R4120:Or8b9	UTSW	9	37,766,705 (GRCm39)	missense	possibly damaging	0.66
R4507:Or8b9	UTSW	9	37,766,201 (GRCm39)	missense	possibly damaging	0.90
R4900:Or8b9	UTSW	9	37,766,608 (GRCm39)	missense	probably benign
R5406:Or8b9	UTSW	9	37,766,515 (GRCm39)	missense	probably benign	0.02
R6813:Or8b9	UTSW	9	37,766,810 (GRCm39)	missense	possibly damaging	0.83
R7061:Or8b9	UTSW	9	37,766,942 (GRCm39)	missense	possibly damaging	0.88
R7315:Or8b9	UTSW	9	37,766,543 (GRCm39)	missense	probably benign
R7500:Or8b9	UTSW	9	37,766,314 (GRCm39)	missense	probably damaging	1.00
R8021:Or8b9	UTSW	9	37,766,592 (GRCm39)	missense	probably damaging	1.00
R8188:Or8b9	UTSW	9	37,766,407 (GRCm39)	missense	probably benign	0.01
R9093:Or8b9	UTSW	9	37,766,294 (GRCm39)	missense	probably damaging	1.00
R9331:Or8b9	UTSW	9	37,766,710 (GRCm39)	missense	probably benign	0.03
R9696:Or8b9	UTSW	9	37,766,671 (GRCm39)	missense	probably benign	0.35
Z1088:Or8b9	UTSW	9	37,766,614 (GRCm39)	missense	probably benign	0.31
Z1177:Or8b9	UTSW	9	37,766,794 (GRCm39)	missense	possibly damaging	0.72

Predicted Primers

PCR Primer
(F):5'- GTCTCCCCAAGTGTGTTCATAC -3'
(R):5'- CCCTGATCCAGAGGCAAACTAG -3'

Sequencing Primer
(F):5'- CAAGTGTGTTCATACCTTTTGTCAG -3'
(R):5'- ACTAGAAGGTGGTTTGAGGTAC -3'

Posted On

2022-04-18