Mutagenetix > Incidental Mutation

Incidental Mutation 'R9373:Olfr1474'

709467

Institutional Source

Beutler Lab

Gene Symbol

Olfr1474

Ensembl Gene

ENSMUSG00000096273

Gene Name

olfactory receptor 1474

Synonyms

MOR202-42, MOR202-26P, GA_x6K02T2RE5P-3803583-3804527

MMRRC Submission

Accession Numbers

Genbank: NM_001011842.1

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R9373 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

13469565-13472157 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 13471852 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 294 (E294G)

Ref Sequence

ENSEMBL: ENSMUSP00000151810 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096202] [ENSMUST00000207529] [ENSMUST00000220113]

AlphaFold

Q7TQQ8

Predicted Effect

probably damaging
Transcript: ENSMUST00000096202
AA Change: E294G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000093916
Gene: ENSMUSG00000096273
AA Change: E294G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	1.6e-52	PFAM
Pfam:7TM_GPCR_Srsx	33	303	1e-7	PFAM
Pfam:7tm_1	39	288	8.7e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000207529

Predicted Effect

probably damaging
Transcript: ENSMUST00000220113
AA Change: E294G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

96% (53/55)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

none

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2200002D01Rik	CCTTCTCCTTCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC	CCTTCTCCTTCTTCTCCTTCTTCTCCATCTTCTCCTTCTTC	7: 29,247,623		probably benign	Het
2310003L06Rik	A	C	5: 87,972,809	D475A	probably benign	Het
3110043O21Rik	A	G	4: 35,196,985	F360S		Het
Aatk	T	C	11: 120,015,517	M243V	possibly damaging	Het
Acox1	A	T	11: 116,174,347	N627K	possibly damaging	Het
Adal	A	G	2: 121,150,222	Y142C	probably benign	Het
Agfg2	A	G	5: 137,664,214		probably null	Het
Ajap1	C	T	4: 153,432,213	A224T	probably benign	Het
Alg1	T	C	16: 5,239,126	F234L	probably benign	Het
Arhgap27	G	T	11: 103,360,461	A147E	possibly damaging	Het
Cachd1	T	C	4: 100,974,870	V743A	possibly damaging	Het
Cc2d2b	G	A	19: 40,795,723	V655I	unknown	Het
Cd72	T	C	4: 43,450,141	S256G	possibly damaging	Het
Celf2	G	T	2: 6,547,104	N521K	probably benign	Het
Clca4a	T	A	3: 144,966,372	N270Y	possibly damaging	Het
Clvs2	A	T	10: 33,528,386	V278E	probably benign	Het
Cwf19l2	T	C	9: 3,454,718	F677S	probably damaging	Het
Dusp9	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	X: 73,640,611		probably benign	Het
Dyx1c1	A	G	9: 72,964,180	T241A	probably damaging	Het
Epdr1	T	C	13: 19,594,537	N130D	possibly damaging	Het
Fam184a	G	A	10: 53,690,019	R491W	probably benign	Het
Fam71e2	T	C	7: 4,757,713	M667V		Het
Foxp2	A	G	6: 15,377,970	Q160R	unknown	Het
Fstl5	T	A	3: 76,648,362	Y515*	probably null	Het
Helz2	G	T	2: 181,240,948	N17K	probably benign	Het
Jmjd1c	C	T	10: 67,096,716		probably benign	Het
Jup	C	T	11: 100,379,565	C372Y	probably damaging	Het
Lrrn1	A	C	6: 107,568,504	Q421P	possibly damaging	Het
Mapk1	A	G	16: 17,018,290	I101V	probably benign	Het
Me2	T	C	18: 73,785,729	E427G	probably damaging	Het
Mrc1	G	A	2: 14,270,188	M433I	probably damaging	Het
Mrvi1	A	G	7: 110,945,831		probably null	Het
Nelfb	G	A	2: 25,205,206	R324W	probably damaging	Het
Nlrp4b	T	A	7: 10,715,199	I443K	probably benign	Het
Nup210l	C	T	3: 90,199,866	P1570L	probably benign	Het
Olfr1012	A	T	2: 85,759,931	Y148*	probably null	Het
Olfr877	T	A	9: 37,855,454	V212D	probably damaging	Het
Olfr914	G	A	9: 38,606,846	C127Y	possibly damaging	Het
Parvb	C	T	15: 84,303,899	T281I	probably damaging	Het
Prune2	A	G	19: 17,122,138	T1669A	probably benign	Het
Runx3	A	G	4: 135,121,145	T14A	probably benign	Het
Scn9a	A	G	2: 66,483,917	V1819A	probably benign	Het
Senp1	T	C	15: 98,066,554	T260A	probably benign	Het
Serpinb10	A	G	1: 107,547,019	R304G	possibly damaging	Het
Spem1	C	T	11: 69,821,814	C65Y	probably benign	Het
Tdrd6	A	G	17: 43,628,162	V665A	possibly damaging	Het
Tenm2	C	T	11: 36,039,886	A1772T	probably damaging	Het
Tenm3	C	T	8: 48,299,655	V891I	probably damaging	Het
Trank1	A	G	9: 111,365,191	D761G	probably benign	Het
Ubap2l	A	T	3: 90,008,280	Y985N	unknown	Het
Vwc2l	A	T	1: 70,729,059	H94L	probably damaging	Het
Wdr27	G	A	17: 14,934,533	H41Y	probably benign	Het
Zcchc6	T	C	13: 59,796,867	S1053G	probably damaging	Het
Zfp7	TGCGGGAAAGGTTTCCACCTGAGCG	TGCG	15: 76,890,600		probably benign	Het
Zfp7	AGTGCGGGAAAGGTTTCCACCTG	AG	15: 76,890,598		probably benign	Het

Other mutations in Olfr1474

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03256:Olfr1474	APN	19	13471267	missense	probably damaging	0.99
D605:Olfr1474	UTSW	19	13471157	nonsense	probably null
R0173:Olfr1474	UTSW	19	13471701	missense	probably benign	0.02
R1102:Olfr1474	UTSW	19	13471407	missense	probably damaging	0.97
R1515:Olfr1474	UTSW	19	13471680	missense	probably damaging	0.97
R1780:Olfr1474	UTSW	19	13471362	missense	probably benign	0.14
R2061:Olfr1474	UTSW	19	13471241	missense	probably damaging	0.98
R4016:Olfr1474	UTSW	19	13471197	missense	possibly damaging	0.95
R4485:Olfr1474	UTSW	19	13471555	missense	probably benign	0.08
R5119:Olfr1474	UTSW	19	13471546	missense	probably benign	0.00
R5150:Olfr1474	UTSW	19	13471430	missense	probably benign	0.01
R5156:Olfr1474	UTSW	19	13471673	missense	probably damaging	1.00
R5699:Olfr1474	UTSW	19	13470972	start codon destroyed	probably null	0.78
R5800:Olfr1474	UTSW	19	13471896	missense	probably benign	0.06
R5840:Olfr1474	UTSW	19	13471878	missense	probably benign	0.01
R5953:Olfr1474	UTSW	19	13471368	missense	possibly damaging	0.92
R5997:Olfr1474	UTSW	19	13471506	missense	probably benign	0.12
R6233:Olfr1474	UTSW	19	13471740	missense	probably damaging	1.00
R6488:Olfr1474	UTSW	19	13471617	missense	probably damaging	1.00
R6847:Olfr1474	UTSW	19	13471038	missense	probably benign	0.03
R6964:Olfr1474	UTSW	19	13471361	nonsense	probably null
R7214:Olfr1474	UTSW	19	13470973	start codon destroyed	probably null	1.00
R8001:Olfr1474	UTSW	19	13471422	missense	probably benign	0.03
R8035:Olfr1474	UTSW	19	13471899	missense	probably benign
R8129:Olfr1474	UTSW	19	13471144	missense	probably damaging	1.00
R9018:Olfr1474	UTSW	19	13471357	missense	possibly damaging	0.60
R9061:Olfr1474	UTSW	19	13471159	missense	probably damaging	0.98
R9065:Olfr1474	UTSW	19	13471306	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GCTATCCTGAGGATGCGTTC -3'
(R):5'- CCAATGCATGAAGTCAAAGTTCTC -3'

Sequencing Primer
(F):5'- AGGAAGGCCTTCTCTACCTGTG -3'
(R):5'- CATTTCACAGGCTTGAGAAA -3'

Posted On

2022-04-18