Mutagenetix > Incidental Mutation

Incidental Mutation 'R9374:Tns3'

709501

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

F830010I22Rik, Tens1, TEM6

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.327) question?

Stock #

R9374 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

8381652-8614681 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 8442606 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 586 (T586S)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000020695
AA Change: T586S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: T586S

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap21	A	T	2: 20,886,397 (GRCm39)	V270E	probably damaging	Het
Blvrb	A	G	7: 27,158,786 (GRCm39)	D62G	probably benign	Het
Ccdc68	T	A	18: 70,089,113 (GRCm39)	S219T	probably damaging	Het
Celf2	G	A	2: 6,590,886 (GRCm39)	T303I	possibly damaging	Het
Cep290	T	C	10: 100,372,729 (GRCm39)	S1176P	probably damaging	Het
Cpeb2	C	A	5: 43,391,584 (GRCm39)	S260*	probably null	Het
Dscam	A	T	16: 96,857,857 (GRCm39)	I51N	probably benign	Het
Erbb4	G	A	1: 68,779,642 (GRCm39)	Q45*	probably null	Het
Fam124a	T	G	14: 62,843,988 (GRCm39)	S499A	possibly damaging	Het
Hipk3	T	C	2: 104,301,850 (GRCm39)	N114S	probably benign	Het
Hnrnph3	T	C	10: 62,853,957 (GRCm39)	M54V	probably benign	Het
Map7d1	T	C	4: 126,127,429 (GRCm39)	E720G	unknown	Het
Mark2	G	T	19: 7,263,263 (GRCm39)	T201N	possibly damaging	Het
Mef2c	A	G	13: 83,810,461 (GRCm39)	N371S	probably benign	Het
Mgst3	T	C	1: 167,205,871 (GRCm39)	Y36C	probably damaging	Het
Mtor	C	T	4: 148,599,397 (GRCm39)	R1482C	probably damaging	Het
Ndst1	G	T	18: 60,845,931 (GRCm39)	T126K	probably damaging	Het
Nkain3	C	A	4: 20,778,431 (GRCm39)		probably benign	Het
Obscn	T	C	11: 58,922,787 (GRCm39)	I5850V	possibly damaging	Het
Or11g1	C	T	14: 50,651,625 (GRCm39)	S208F	probably benign	Het
Or5d14	C	T	2: 87,880,509 (GRCm39)	W153*	probably null	Het
Or6k4	A	T	1: 173,964,885 (GRCm39)	T192S	probably benign	Het
Pbx1	T	C	1: 168,258,910 (GRCm39)	D55G	possibly damaging	Het
Pi4ka	T	C	16: 17,125,574 (GRCm39)	E1187G		Het
Pkn1	A	T	8: 84,404,367 (GRCm39)	N543K	probably benign	Het
Plin1	T	C	7: 79,372,544 (GRCm39)	N343D	probably benign	Het
Prpf4	T	C	4: 62,336,131 (GRCm39)	V286A	probably benign	Het
Pyroxd2	A	G	19: 42,719,756 (GRCm39)		probably null	Het
Rbpms	A	G	8: 34,294,173 (GRCm39)	F190S	possibly damaging	Het
Rpl10a	T	A	17: 28,548,006 (GRCm39)	F49Y	possibly damaging	Het
Slc32a1	A	G	2: 158,455,995 (GRCm39)	T217A	probably damaging	Het
Slco6c1	T	C	1: 97,055,827 (GRCm39)	S25G	probably benign	Het
Slitrk5	C	T	14: 111,916,496 (GRCm39)	T40I	probably benign	Het
Smchd1	T	C	17: 71,718,843 (GRCm39)	N842S	possibly damaging	Het
Sorbs1	G	A	19: 40,361,923 (GRCm39)	R154*	probably null	Het
Stc2	A	G	11: 31,310,332 (GRCm39)	S235P	probably benign	Het
Tcaf2	C	T	6: 42,619,728 (GRCm39)	A100T	probably benign	Het
Tcerg1l	T	C	7: 137,995,998 (GRCm39)	D170G	possibly damaging	Het
Tcf15	T	C	2: 151,986,039 (GRCm39)	L165P	probably damaging	Het
Tle5	T	C	10: 81,399,988 (GRCm39)	V62A	probably damaging	Het
Tmem200c	G	T	17: 69,148,682 (GRCm39)	G422W	probably damaging	Het
Tnfsf13b	A	G	8: 10,085,391 (GRCm39)	I294V	possibly damaging	Het
Unc80	A	T	1: 66,629,460 (GRCm39)	E1319V	possibly damaging	Het
Vegfc	A	G	8: 54,622,180 (GRCm39)	I221V	possibly damaging	Het
Vmn1r86	A	T	7: 12,836,781 (GRCm39)	Y32N	possibly damaging	Het
Vmn2r111	T	A	17: 22,787,859 (GRCm39)	L497F	probably benign	Het
Wdr49	T	C	3: 75,230,931 (GRCm39)	D577G	probably benign	Het
Xpo4	A	G	14: 57,828,512 (GRCm39)	F783L	possibly damaging	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8,401,066 (GRCm39)	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8,393,976 (GRCm39)	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8,428,399 (GRCm39)	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8,442,617 (GRCm39)	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8,498,937 (GRCm39)	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8,442,798 (GRCm39)	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8,469,192 (GRCm39)	splice site	probably benign
IGL01844:Tns3	APN	11	8,387,177 (GRCm39)	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8,498,992 (GRCm39)	nonsense	probably null
IGL02137:Tns3	APN	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8,387,141 (GRCm39)	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8,442,346 (GRCm39)	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8,469,564 (GRCm39)	missense	probably damaging	1.00
ANU74:Tns3	UTSW	11	8,442,149 (GRCm39)	missense	probably benign	0.38
R0020:Tns3	UTSW	11	8,495,227 (GRCm39)	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0370:Tns3	UTSW	11	8,395,730 (GRCm39)	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8,395,703 (GRCm39)	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8,385,852 (GRCm39)	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8,497,262 (GRCm39)	splice site	probably benign
R0562:Tns3	UTSW	11	8,443,262 (GRCm39)	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8,443,121 (GRCm39)	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8,469,474 (GRCm39)	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8,443,302 (GRCm39)	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8,398,704 (GRCm39)	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8,468,261 (GRCm39)	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8,385,738 (GRCm39)	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8,481,719 (GRCm39)	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8,491,200 (GRCm39)	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8,385,870 (GRCm39)	missense	probably damaging	1.00
R3720:Tns3	UTSW	11	8,442,999 (GRCm39)	missense	probably damaging	1.00
R3765:Tns3	UTSW	11	8,401,133 (GRCm39)	missense	probably benign	0.00
R3817:Tns3	UTSW	11	8,384,619 (GRCm39)	missense	probably damaging	1.00
R4058:Tns3	UTSW	11	8,442,275 (GRCm39)	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8,481,747 (GRCm39)	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8,401,119 (GRCm39)	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8,401,092 (GRCm39)	missense	probably benign
R5749:Tns3	UTSW	11	8,401,177 (GRCm39)	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8,443,211 (GRCm39)	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8,384,580 (GRCm39)	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8,385,860 (GRCm39)	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8,442,245 (GRCm39)	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8,442,987 (GRCm39)	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8,442,147 (GRCm39)	missense	probably benign	0.01
R6536:Tns3	UTSW	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,058 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,057 (GRCm39)	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8,443,196 (GRCm39)	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8,481,743 (GRCm39)	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8,387,251 (GRCm39)	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8,401,442 (GRCm39)	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8,442,793 (GRCm39)	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8,480,894 (GRCm39)	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8,491,192 (GRCm39)	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8,442,701 (GRCm39)	missense	probably benign	0.01
R8055:Tns3	UTSW	11	8,495,343 (GRCm39)	missense	probably damaging	1.00
R8057:Tns3	UTSW	11	8,442,773 (GRCm39)	missense	probably benign
R8077:Tns3	UTSW	11	8,395,667 (GRCm39)	missense	probably damaging	1.00
R8518:Tns3	UTSW	11	8,442,971 (GRCm39)	missense	probably damaging	0.96
R8523:Tns3	UTSW	11	8,398,779 (GRCm39)	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8,468,273 (GRCm39)	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8,400,094 (GRCm39)	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
T0975:Tns3	UTSW	11	8,401,146 (GRCm39)	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8,499,100 (GRCm39)	start gained	probably benign
X0005:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,401,224 (GRCm39)	missense	probably benign	0.00
Z1177:Tns3	UTSW	11	8,401,014 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATTGCTGATGCCTCTCTGAAC -3'
(R):5'- AGCCAGAACTCTCTCCTGTC -3'

Sequencing Primer
(F):5'- TGATGCCTCTCTGAACAGCCAC -3'
(R):5'- AGAACTCTCTCCTGTCTGATGG -3'

Posted On

2022-04-18