Mutagenetix > Incidental Mutation

Incidental Mutation 'R9375:Vmn2r12'

709537

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R9375 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

109085849-109097864 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 109086120 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 742 (H742L)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

probably damaging
Transcript: ENSMUST00000095922
AA Change: H742L

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: H742L

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

99% (72/73)

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900026A02Rik	T	C	5: 113,184,701	D549G	probably benign	Het
Aamdc	A	G	7: 97,550,695	V120A	probably benign	Het
Acp2	A	T	2: 91,206,829	Q251L	probably benign	Het
AI597479	C	T	1: 43,111,345	A205V	possibly damaging	Het
Apob	A	G	12: 7,979,261	T44A	possibly damaging	Het
Arhgap18	G	A	10: 26,772,614	V11M	probably damaging	Het
Arhgef10l	T	G	4: 140,591,954	H156P	probably benign	Het
Aste1	G	A	9: 105,396,681	C40Y	probably benign	Het
BC053393	T	A	11: 46,577,419	C107S	probably damaging	Het
Chd9	G	A	8: 90,998,707		probably null	Het
Clec4n	T	A	6: 123,230,703	F42L	probably benign	Het
Cngb1	T	C	8: 95,299,722	K144E	unknown	Het
Ctgf	A	T	10: 24,597,603	M347L	possibly damaging	Het
Cyp4f17	A	G	17: 32,528,772	T485A	probably damaging	Het
Dcaf6	T	A	1: 165,357,483	Y480F	probably damaging	Het
Defa30	C	T	8: 21,134,670	T3I	probably damaging	Het
Dmxl1	T	A	18: 49,958,410	W2854R	probably damaging	Het
Dnah11	G	A	12: 117,920,968	T3783I	possibly damaging	Het
Dync1i1	T	A	6: 5,913,443	F225L	possibly damaging	Het
Elp6	T	A	9: 110,315,784	Y119*	probably null	Het
Enpep	A	T	3: 129,331,880	M201K	probably benign	Het
Fam161a	A	T	11: 23,020,661	R280W	probably damaging	Het
Fam98a	T	C	17: 75,541,335	N128S	possibly damaging	Het
Fpgt	A	T	3: 155,087,297	D364E	probably benign	Het
G3bp1	T	A	11: 55,499,613	M448K	probably damaging	Het
Gadd45b	T	C	10: 80,930,450	M16T	probably benign	Het
Gdi2	A	T	13: 3,564,869	I383F	probably benign	Het
Gm9268	C	T	7: 43,024,075	P193S	possibly damaging	Het
Gm9833	T	C	3: 10,088,731	S187P	probably benign	Het
H2-Bl	T	C	17: 36,081,101	T27A	possibly damaging	Het
Igtp	T	G	11: 58,206,200	L66V	probably damaging	Het
Kcne4	G	A	1: 78,817,906	M90I	probably benign	Het
Lmo7	T	A	14: 101,898,687	M732K	probably damaging	Het
Ltbp2	G	A	12: 84,791,090	P1192L	probably benign	Het
Mast4	T	G	13: 102,781,245	S552R	probably damaging	Het
Mff	A	T	1: 82,729,286	M11L	probably benign	Het
Mterf2	T	C	10: 85,120,463	E99G	probably damaging	Het
Myom2	T	A	8: 15,099,210	N560K	probably damaging	Het
Nab2	A	G	10: 127,664,711	S171P	probably damaging	Het
Nhlrc3	T	C	3: 53,461,769	N83S	possibly damaging	Het
Nod2	A	T	8: 88,664,405	I447F	probably damaging	Het
Npat	T	A	9: 53,563,156	D749E	possibly damaging	Het
Nxph4	A	G	10: 127,526,762	S87P	probably benign	Het
Olfr1392	T	A	11: 49,294,075	Y251*	probably null	Het
Olfr1497	T	A	19: 13,794,850	T254S	probably damaging	Het
Olfr641	G	T	7: 104,040,066	R90L	possibly damaging	Het
Olfr74	G	A	2: 87,974,182	T161M	possibly damaging	Het
Olfr782	T	A	10: 129,351,120	S186T	probably damaging	Het
Pcdha3	G	A	18: 36,946,300	V32I	probably benign	Het
Pcdhgc3	A	T	18: 37,806,638	T31S	probably benign	Het
Phldb3	A	G	7: 24,623,872	R391G	probably damaging	Het
Piezo1	A	G	8: 122,501,865	V257A		Het
Pip5k1b	T	A	19: 24,439,078	Q15L	probably benign	Het
Plxnc1	T	C	10: 94,813,231	N1229D	probably benign	Het
Ptpn12	A	G	5: 21,019,214	V127A	probably benign	Het
Ptprr	T	A	10: 116,273,819	V655D	probably benign	Het
Rbbp8	A	G	18: 11,705,831	T190A	probably benign	Het
Rnf17	T	A	14: 56,482,122	V943E	probably damaging	Het
Rnpc3	C	T	3: 113,611,264	G414D	probably damaging	Het
Robo4	T	A	9: 37,404,862	N387K	probably damaging	Het
Sall4	G	A	2: 168,755,861	T353M	probably damaging	Het
Serpinb13	T	C	1: 106,982,267	I38T	probably damaging	Het
Sgsm1	A	T	5: 113,274,273	I218N	unknown	Het
Sntg2	T	A	12: 30,243,344		probably null	Het
Taf1c	G	A	8: 119,598,654	S823F	probably damaging	Het
Tldc1	G	A	8: 119,771,357	A136V	probably benign	Het
Trhde	T	G	10: 114,408,693	I963L	probably damaging	Het
Trim43b	T	C	9: 89,085,566	Y339C	probably damaging	Het
Trp53	T	A	11: 69,589,711		probably null	Het
Tubg2	T	C	11: 101,160,795	V282A	probably benign	Het
Ube2g1	C	T	11: 72,663,117		probably benign	Het
Usp34	C	A	11: 23,487,203	A3341E		Het
Vmn2r24	T	C	6: 123,815,583	V623A	probably damaging	Het
Vmn2r5	A	T	3: 64,503,895	D417E	probably damaging	Het
Xirp2	A	T	2: 67,511,774	D1453V	probably damaging	Het
Zfp2	T	C	11: 50,900,215	S334G	probably damaging	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109097675	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109086259	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109091850	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109093027	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109086564	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109092159	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109086477	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109085992	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109090485	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109092070	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109092899	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109091889	nonsense	probably null
R0529:Vmn2r12	UTSW	5	109092848	missense	probably benign
R0715:Vmn2r12	UTSW	5	109090507	missense	probably benign	0.10
R0742:Vmn2r12	UTSW	5	109086415	missense	possibly damaging	0.55
R0894:Vmn2r12	UTSW	5	109087850	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109092854	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109092854	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109091897	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109086586	missense	probably benign	0.00
R1388:Vmn2r12	UTSW	5	109092974	missense	possibly damaging	0.56
R1549:Vmn2r12	UTSW	5	109092830	missense	probably benign	0.06
R1766:Vmn2r12	UTSW	5	109092044	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109091728	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109092076	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109091474	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109091531	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109091531	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109090504	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109092192	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109092192	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109091546	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109086435	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109086513	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109092986	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109091678	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109091506	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109090395	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109091818	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109086617	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109092800	missense	probably benign	0.06
R5753:Vmn2r12	UTSW	5	109091804	missense	probably damaging	1.00
R5797:Vmn2r12	UTSW	5	109085870	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109092897	missense	probably benign
R6162:Vmn2r12	UTSW	5	109086564	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109086000	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109092905	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109097789	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109086247	missense	possibly damaging	0.74
R7341:Vmn2r12	UTSW	5	109091945	missense	possibly damaging	0.95
R7383:Vmn2r12	UTSW	5	109092818	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109091749	missense	probably damaging	1.00
R7749:Vmn2r12	UTSW	5	109086054	missense	probably damaging	0.99
R7861:Vmn2r12	UTSW	5	109087963	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109086441	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109091881	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109090483	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109086208	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109092086	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109086656	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109086167	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109086019	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109093044	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109086337	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109086586	missense	probably benign	0.00
R9524:Vmn2r12	UTSW	5	109091957	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109091456	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109092780	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109091437	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCTGCACTAGAAGCCAAGATG -3'
(R):5'- CTACTCCTGGGAGAAGGATGAG -3'

Sequencing Primer
(F):5'- CTAGAAGCCAAGATGGAGAAAACCTC -3'
(R):5'- GATGAGAGGGATGCTGGC -3'

Posted On

2022-04-18