Mutagenetix > Incidental Mutation

Incidental Mutation 'R9376:Oas1f'

709614

Institutional Source

Beutler Lab

Gene Symbol

Oas1f

Ensembl Gene

ENSMUSG00000053765

Gene Name

2'-5' oligoadenylate synthetase 1F

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R9376 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

120985432-120996049 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 120986243 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 65 (Y65*)

Ref Sequence

ENSEMBL: ENSMUSP00000056993 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057814]

AlphaFold

Q14AZ4

Predicted Effect

probably null
Transcript: ENSMUST00000057814
AA Change: Y65*

SMART Domains

Protein: ENSMUSP00000056993
Gene: ENSMUSG00000053765
AA Change: Y65*

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	169	353	4.6e-81	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000123431

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	T	A	7: 119,893,438 (GRCm39)	L1296H	probably damaging	Het
Abcb4	A	T	5: 9,008,988 (GRCm39)	D1215V	probably damaging	Het
Ache	A	C	5: 137,289,025 (GRCm39)	I244L	probably benign	Het
Ankrd13d	A	T	19: 4,332,250 (GRCm39)	V58D	probably damaging	Het
Ano3	A	G	2: 110,496,782 (GRCm39)	L789S	probably damaging	Het
Atp2a3	T	C	11: 72,863,290 (GRCm39)	V129A	probably damaging	Het
Cldn11	T	C	3: 31,217,410 (GRCm39)	S193P	possibly damaging	Het
Clpb	A	T	7: 101,360,625 (GRCm39)	R218S	probably benign	Het
Cntln	A	G	4: 84,875,258 (GRCm39)	S231G	probably benign	Het
Cntn4	T	C	6: 106,639,591 (GRCm39)	Y574H	probably damaging	Het
Col6a3	C	T	1: 90,709,523 (GRCm39)	R2498H	unknown	Het
Ctdnep1	T	C	11: 69,875,594 (GRCm39)	V108A	probably damaging	Het
Cyp2c67	A	T	19: 39,627,178 (GRCm39)	N217K	probably damaging	Het
Dao	T	A	5: 114,147,901 (GRCm39)	M1K	probably null	Het
Dchs1	A	G	7: 105,414,981 (GRCm39)		probably null	Het
Dennd4a	A	T	9: 64,819,974 (GRCm39)	I1809L	probably benign	Het
Dnah7a	T	A	1: 53,568,058 (GRCm39)		probably null	Het
Epn1	A	T	7: 5,086,720 (GRCm39)		probably benign	Het
Erich1	A	G	8: 14,080,719 (GRCm39)	S267P	probably damaging	Het
Gm17732	G	T	18: 62,795,936 (GRCm39)	V41L	probably damaging	Het
Gpaa1	C	A	15: 76,218,826 (GRCm39)	T556K	possibly damaging	Het
Gpr65	T	A	12: 98,241,523 (GRCm39)	S59T	probably damaging	Het
Hydin	T	C	8: 111,124,695 (GRCm39)	V568A	possibly damaging	Het
Ifi205	T	C	1: 173,854,221 (GRCm39)	D144G	probably benign	Het
Itga1	T	C	13: 115,107,112 (GRCm39)	H993R	probably benign	Het
Itgax	T	C	7: 127,747,935 (GRCm39)	L1075P	possibly damaging	Het
Itpr1	T	A	6: 108,326,638 (GRCm39)	V120E	probably damaging	Het
Kdm3b	T	C	18: 34,970,718 (GRCm39)	S1762P	probably damaging	Het
Krt80	T	A	15: 101,247,978 (GRCm39)	T221S	unknown	Het
Lama2	T	C	10: 26,994,620 (GRCm39)	D1763G	probably benign	Het
Lrp5	T	C	19: 3,670,286 (GRCm39)	D606G	probably benign	Het
Map6d1	T	C	16: 20,059,933 (GRCm39)	D44G	probably benign	Het
Mtmr11	T	A	3: 96,072,372 (GRCm39)	I282K	probably benign	Het
Nalcn	G	A	14: 123,515,713 (GRCm39)	T1696M	possibly damaging	Het
Nup214	T	C	2: 31,924,244 (GRCm39)	V1591A	probably benign	Het
Or1x6	T	A	11: 50,939,662 (GRCm39)	C243S	probably damaging	Het
Or2t43	T	A	11: 58,457,683 (GRCm39)	T163S	possibly damaging	Het
Or2t46	T	C	11: 58,472,636 (GRCm39)	V322A	probably benign	Het
Or51f1e	A	T	7: 102,746,971 (GRCm39)	T8S	probably benign	Het
Or5p58	A	T	7: 107,694,471 (GRCm39)	I102K	possibly damaging	Het
Palld	C	T	8: 61,969,691 (GRCm39)	R1211H	unknown	Het
Pigyl	A	G	9: 22,069,324 (GRCm39)	I12V	probably benign	Het
Pno1	A	T	11: 17,158,791 (GRCm39)	D202E	probably benign	Het
Ppp5c	A	T	7: 16,743,849 (GRCm39)	M195K	probably damaging	Het
Prrc2a	A	T	17: 35,369,598 (GRCm39)	F1905Y	possibly damaging	Het
Ptpn11	C	A	5: 121,282,681 (GRCm39)	D435Y	probably damaging	Het
Rbm33	A	G	5: 28,544,164 (GRCm39)	Y140C	probably damaging	Het
Runx1t1	A	G	4: 13,865,225 (GRCm39)	E339G	possibly damaging	Het
Scube3	G	T	17: 28,383,670 (GRCm39)	K480N	possibly damaging	Het
Sephs1	T	A	2: 4,910,469 (GRCm39)	M313K	probably benign	Het
Tcaim	T	C	9: 122,655,995 (GRCm39)	W304R	probably damaging	Het
Terf2ip	C	T	8: 112,738,514 (GRCm39)	T134I	probably damaging	Het
Terf2ip	A	G	8: 112,744,528 (GRCm39)	T282A	probably benign	Het
Tnfrsf18	G	T	4: 156,112,448 (GRCm39)	A102S	probably benign	Het
Trim10	A	T	17: 37,184,168 (GRCm39)	I254F	probably benign	Het
Triqk	A	G	4: 12,962,987 (GRCm39)	T9A	probably benign	Het
Tshz2	G	A	2: 169,726,013 (GRCm39)	S203N	probably benign	Het
Zc3h13	G	A	14: 75,561,128 (GRCm39)	V573I	unknown	Het
Zfhx4	A	G	3: 5,306,833 (GRCm39)	K20E	probably damaging	Het
Zfhx4	A	G	3: 5,465,395 (GRCm39)	D1876G	probably benign	Het

Other mutations in Oas1f

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02398:Oas1f	APN	5	120,989,568 (GRCm39)	missense	probably benign
R0378:Oas1f	UTSW	5	120,994,489 (GRCm39)	missense	probably damaging	0.99
R1414:Oas1f	UTSW	5	120,989,522 (GRCm39)	intron	probably benign
R1826:Oas1f	UTSW	5	120,993,652 (GRCm39)	missense	probably benign	0.33
R1848:Oas1f	UTSW	5	120,993,492 (GRCm39)	missense	probably damaging	1.00
R1895:Oas1f	UTSW	5	120,993,648 (GRCm39)	missense	probably benign	0.04
R1960:Oas1f	UTSW	5	120,994,502 (GRCm39)	missense	possibly damaging	0.54
R2193:Oas1f	UTSW	5	120,989,648 (GRCm39)	missense	probably damaging	1.00
R4622:Oas1f	UTSW	5	120,986,390 (GRCm39)	missense	probably damaging	0.98
R4758:Oas1f	UTSW	5	120,985,545 (GRCm39)	missense	probably damaging	1.00
R5882:Oas1f	UTSW	5	120,986,316 (GRCm39)	missense	probably damaging	0.99
R6515:Oas1f	UTSW	5	120,986,497 (GRCm39)	missense	probably damaging	1.00
R6944:Oas1f	UTSW	5	120,986,247 (GRCm39)	missense	probably benign	0.01
R7980:Oas1f	UTSW	5	120,989,538 (GRCm39)	missense	probably benign	0.16
R8048:Oas1f	UTSW	5	120,986,479 (GRCm39)	missense	possibly damaging	0.91
R8048:Oas1f	UTSW	5	120,985,602 (GRCm39)	missense	probably damaging	1.00
R8921:Oas1f	UTSW	5	120,989,556 (GRCm39)	missense	probably benign	0.20
R9453:Oas1f	UTSW	5	120,993,592 (GRCm39)	missense	probably damaging	0.99
R9777:Oas1f	UTSW	5	120,994,429 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATTCGCTCTGTGACCTTTGAG -3'
(R):5'- AAGCTCAGAGACCTGGAGTTGG -3'

Sequencing Primer
(F):5'- TGACCTTTGAGTTTTGGTACATAC -3'
(R):5'- TCTGAGCTCTGCACCTCAAAC -3'

Posted On

2022-04-18