Mutagenetix > Incidental Mutations

Incidental Mutation 'R9376:Cyp2c67'

709656

Institutional Source

Beutler Lab

Gene Symbol

Cyp2c67

Ensembl Gene

ENSMUSG00000062624

Gene Name

cytochrome P450, family 2, subfamily c, polypeptide 67

Synonyms

C730004C24Rik

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R9376 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

39608842-39649051 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 39638734 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 217 (N217K)

Ref Sequence

ENSEMBL: ENSMUSP00000065796 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067328]

AlphaFold

Q569X9

Predicted Effect

probably damaging
Transcript: ENSMUST00000067328
AA Change: N217K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000065796
Gene: ENSMUSG00000062624
AA Change: N217K

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
Pfam:p450	30	487	8.5e-150	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	T	A	7: 120,294,215	L1296H	probably damaging	Het
Abcb4	A	T	5: 8,958,988	D1215V	probably damaging	Het
Ache	A	C	5: 137,290,763	I244L	probably benign	Het
Ankrd13d	A	T	19: 4,282,222	V58D	probably damaging	Het
Ano3	A	G	2: 110,666,437	L789S	probably damaging	Het
Atp2a3	T	C	11: 72,972,464	V129A	probably damaging	Het
Cldn11	T	C	3: 31,163,261	S193P	possibly damaging	Het
Clpb	A	T	7: 101,711,418	R218S	probably benign	Het
Cntln	A	G	4: 84,957,021	S231G	probably benign	Het
Cntn4	T	C	6: 106,662,630	Y574H	probably damaging	Het
Col6a3	C	T	1: 90,781,801	R2498H	unknown	Het
Ctdnep1	T	C	11: 69,984,768	V108A	probably damaging	Het
Dao	T	A	5: 114,009,840	M1K	probably null	Het
Dchs1	A	G	7: 105,765,774		probably null	Het
Dennd4a	A	T	9: 64,912,692	I1809L	probably benign	Het
Dnah7a	T	A	1: 53,528,899		probably null	Het
Epn1	A	T	7: 5,083,721		probably benign	Het
Erich1	A	G	8: 14,030,719	S267P	probably damaging	Het
Gm17732	G	T	18: 62,662,865	V41L	probably damaging	Het
Gpaa1	C	A	15: 76,334,626	T556K	possibly damaging	Het
Gpr65	T	A	12: 98,275,264	S59T	probably damaging	Het
Hydin	T	C	8: 110,398,063	V568A	possibly damaging	Het
Ifi205	T	C	1: 174,026,655	D144G	probably benign	Het
Itga1	T	C	13: 114,970,576	H993R	probably benign	Het
Itgax	T	C	7: 128,148,763	L1075P	possibly damaging	Het
Itpr1	T	A	6: 108,349,677	V120E	probably damaging	Het
Kdm3b	T	C	18: 34,837,665	S1762P	probably damaging	Het
Krt80	T	A	15: 101,350,097	T221S	unknown	Het
Lama2	T	C	10: 27,118,624	D1763G	probably benign	Het
Lrp5	T	C	19: 3,620,286	D606G	probably benign	Het
Map6d1	T	C	16: 20,241,183	D44G	probably benign	Het
Mtmr11	T	A	3: 96,165,055	I282K	probably benign	Het
Nalcn	G	A	14: 123,278,301	T1696M	possibly damaging	Het
Nup214	T	C	2: 32,034,232	V1591A	probably benign	Het
Oas1f	C	A	5: 120,848,180	Y65*	probably null	Het
Olfr1375	T	A	11: 51,048,835	C243S	probably damaging	Het
Olfr224	T	A	11: 58,566,857	T163S	possibly damaging	Het
Olfr325	T	C	11: 58,581,810	V322A	probably benign	Het
Olfr482	A	T	7: 108,095,264	I102K	possibly damaging	Het
Olfr585	A	T	7: 103,097,764	T8S	probably benign	Het
Palld	C	T	8: 61,516,657	R1211H	unknown	Het
Pigyl	A	G	9: 22,158,028	I12V	probably benign	Het
Pno1	A	T	11: 17,208,791	D202E	probably benign	Het
Ppp5c	A	T	7: 17,009,924	M195K	probably damaging	Het
Prrc2a	A	T	17: 35,150,622	F1905Y	possibly damaging	Het
Ptpn11	C	A	5: 121,144,618	D435Y	probably damaging	Het
Rbm33	A	G	5: 28,339,166	Y140C	probably damaging	Het
Runx1t1	A	G	4: 13,865,225	E339G	possibly damaging	Het
Scube3	G	T	17: 28,164,696	K480N	possibly damaging	Het
Sephs1	T	A	2: 4,905,658	M313K	probably benign	Het
Tcaim	T	C	9: 122,826,930	W304R	probably damaging	Het
Terf2ip	C	T	8: 112,011,882	T134I	probably damaging	Het
Terf2ip	A	G	8: 112,017,896	T282A	probably benign	Het
Tnfrsf18	G	T	4: 156,027,991	A102S	probably benign	Het
Trim10	A	T	17: 36,873,276	I254F	probably benign	Het
Triqk	A	G	4: 12,962,987	T9A	probably benign	Het
Tshz2	G	A	2: 169,884,093	S203N	probably benign	Het
Zc3h13	G	A	14: 75,323,688	V573I	unknown	Het
Zfhx4	A	G	3: 5,241,773	K20E	probably damaging	Het
Zfhx4	A	G	3: 5,400,335	D1876G	probably benign	Het

Other mutations in Cyp2c67

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00955:Cyp2c67	APN	19	39643385	missense	possibly damaging	0.95
IGL01025:Cyp2c67	APN	19	39639932	nonsense	probably null
IGL01363:Cyp2c67	APN	19	39639967	missense	probably damaging	0.99
IGL01819:Cyp2c67	APN	19	39615721	missense	probably damaging	0.98
IGL01902:Cyp2c67	APN	19	39649026	missense	probably damaging	1.00
IGL02172:Cyp2c67	APN	19	39649002	missense	possibly damaging	0.76
IGL02351:Cyp2c67	APN	19	39617417	missense	probably damaging	1.00
IGL02355:Cyp2c67	APN	19	39643405	missense	probably benign	0.34
IGL02355:Cyp2c67	APN	19	39617382	nonsense	probably null
IGL02358:Cyp2c67	APN	19	39617417	missense	probably damaging	1.00
IGL02362:Cyp2c67	APN	19	39643405	missense	probably benign	0.34
IGL02362:Cyp2c67	APN	19	39617382	nonsense	probably null
IGL02388:Cyp2c67	APN	19	39643355	missense	probably benign	0.20
IGL03106:Cyp2c67	APN	19	39643675	missense	probably benign	0.27
IGL03219:Cyp2c67	APN	19	39643294	missense	possibly damaging	0.54
IGL03326:Cyp2c67	APN	19	39643269	critical splice donor site	probably null
IGL03349:Cyp2c67	APN	19	39643684	missense	probably damaging	1.00
IGL03356:Cyp2c67	APN	19	39639961	missense	probably damaging	1.00
IGL03052:Cyp2c67	UTSW	19	39648885	missense	possibly damaging	0.88
R0585:Cyp2c67	UTSW	19	39638694	missense	possibly damaging	0.59
R0975:Cyp2c67	UTSW	19	39609178	missense	possibly damaging	0.49
R0976:Cyp2c67	UTSW	19	39643374	missense	probably damaging	1.00
R1252:Cyp2c67	UTSW	19	39626141	missense	possibly damaging	0.93
R1398:Cyp2c67	UTSW	19	39638625	missense	probably damaging	0.96
R1411:Cyp2c67	UTSW	19	39638591	missense	probably damaging	1.00
R1505:Cyp2c67	UTSW	19	39648964	missense	probably benign	0.00
R1543:Cyp2c67	UTSW	19	39643264	splice site	probably benign
R1613:Cyp2c67	UTSW	19	39626199	missense	probably benign	0.00
R1618:Cyp2c67	UTSW	19	39643264	splice site	probably benign
R1667:Cyp2c67	UTSW	19	39643590	critical splice donor site	probably null
R1852:Cyp2c67	UTSW	19	39617367	missense	probably benign	0.01
R2005:Cyp2c67	UTSW	19	39643345	missense	probably damaging	1.00
R2105:Cyp2c67	UTSW	19	39626237	missense	probably benign	0.24
R2181:Cyp2c67	UTSW	19	39609097	missense	possibly damaging	0.94
R3817:Cyp2c67	UTSW	19	39638683	missense	probably benign	0.00
R4669:Cyp2c67	UTSW	19	39643654	missense	probably benign	0.00
R4689:Cyp2c67	UTSW	19	39638588	missense	probably benign	0.00
R4756:Cyp2c67	UTSW	19	39643744	missense	probably benign	0.03
R4823:Cyp2c67	UTSW	19	39615724	missense	probably benign	0.13
R5152:Cyp2c67	UTSW	19	39638688	missense	probably benign	0.00
R5345:Cyp2c67	UTSW	19	39626232	missense	probably benign	0.01
R5580:Cyp2c67	UTSW	19	39615650	missense	probably damaging	0.99
R5644:Cyp2c67	UTSW	19	39615694	missense	possibly damaging	0.84
R6116:Cyp2c67	UTSW	19	39617435	missense	probably damaging	1.00
R6516:Cyp2c67	UTSW	19	39617429	missense	probably damaging	1.00
R6550:Cyp2c67	UTSW	19	39617410	nonsense	probably null
R6939:Cyp2c67	UTSW	19	39643334	missense	possibly damaging	0.68
R6995:Cyp2c67	UTSW	19	39615679	missense	probably damaging	0.96
R7028:Cyp2c67	UTSW	19	39639897	missense	possibly damaging	0.68
R7144:Cyp2c67	UTSW	19	39615694	missense	probably benign	0.00
R7242:Cyp2c67	UTSW	19	39617339	missense	probably benign	0.30
R7335:Cyp2c67	UTSW	19	39640007	nonsense	probably null
R7337:Cyp2c67	UTSW	19	39609264	splice site	probably null
R7474:Cyp2c67	UTSW	19	39617432	missense	probably null	0.05
R7642:Cyp2c67	UTSW	19	39615640	missense	probably damaging	0.97
R7870:Cyp2c67	UTSW	19	39609225	missense	probably damaging	1.00
R8152:Cyp2c67	UTSW	19	39640008	missense	probably benign	0.21
R8367:Cyp2c67	UTSW	19	39638674	missense	probably benign	0.01
R8717:Cyp2c67	UTSW	19	39638711	missense	probably benign	0.05
R8728:Cyp2c67	UTSW	19	39626161	missense	probably damaging	1.00
R9275:Cyp2c67	UTSW	19	39609255	missense	probably damaging	1.00
R9278:Cyp2c67	UTSW	19	39609255	missense	probably damaging	1.00
Z1177:Cyp2c67	UTSW	19	39643679	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- TCAATGAACTCTACAGTCCATGAC -3'
(R):5'- GGTTATTCAAACGTGTAGAACAGAG -3'

Sequencing Primer
(F):5'- TGAACTCTACAGTCCATGACAAGTC -3'
(R):5'- ACACTCAAAAGATATGTGAGTGTG -3'

Posted On

2022-04-18