Mutagenetix > Incidental Mutation

Incidental Mutation 'R9389:Prss3b'

710480

Institutional Source

Beutler Lab

Gene Symbol

Prss3b

Ensembl Gene

ENSMUSG00000029882

Gene Name

serine protease 3B

Synonyms

2210010C04Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R9389 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41007202-41012443 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 41010079 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 85 (G85D)

Ref Sequence

ENSEMBL: ENSMUSP00000031931 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031931]

AlphaFold

Q9CPN9

Predicted Effect

probably benign
Transcript: ENSMUST00000031931
AA Change: G85D

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000031931
Gene: ENSMUSG00000029882
AA Change: G85D

Domain	Start	End	E-Value	Type
Tryp_SPc	24	240	3.52e-102	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (55/55)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1b	G	A	5: 8,875,614 (GRCm39)	V596I	probably benign	Het
Agtpbp1	A	T	13: 59,613,884 (GRCm39)	M1019K	probably damaging	Het
Arfgef3	A	G	10: 18,479,271 (GRCm39)	V1448A	probably damaging	Het
Baz1a	T	A	12: 54,963,608 (GRCm39)	E828D	probably damaging	Het
Ces1f	A	T	8: 93,996,600 (GRCm39)		probably null	Het
Cfap53	T	A	18: 74,432,414 (GRCm39)		probably null	Het
Col6a4	T	C	9: 105,877,983 (GRCm39)	Y1998C	probably damaging	Het
Col9a2	A	G	4: 120,911,948 (GRCm39)	T675A	probably benign	Het
Dusp6	G	A	10: 99,099,839 (GRCm39)	V96M	possibly damaging	Het
Elmo1	T	G	13: 20,369,661 (GRCm39)	M22R	probably benign	Het
Fem1al	A	G	11: 29,775,107 (GRCm39)	S117P	probably damaging	Het
Gm19965	A	G	1: 116,749,566 (GRCm39)	N416D		Het
Gpr162	A	G	6: 124,838,357 (GRCm39)	Y98H	probably damaging	Het
Igfals	G	A	17: 25,100,600 (GRCm39)	V564I	probably benign	Het
Il4	C	T	11: 53,504,837 (GRCm39)	R76H	probably damaging	Het
Itgb1	T	A	8: 129,433,637 (GRCm39)	N50K	probably benign	Het
Itpr3	A	T	17: 27,314,899 (GRCm39)	Y682F	possibly damaging	Het
Jak3	C	T	8: 72,136,696 (GRCm39)	P668S	probably damaging	Het
Malrd1	A	T	2: 15,707,967 (GRCm39)	N932I	unknown	Het
Mast4	C	A	13: 103,470,438 (GRCm39)	R88L	probably benign	Het
Mfsd11	T	G	11: 116,764,161 (GRCm39)	S381A	probably benign	Het
Mrm3	A	G	11: 76,140,856 (GRCm39)	D288G	probably damaging	Het
Myg1	G	T	15: 102,245,372 (GRCm39)	V198F	probably damaging	Het
Naip5	T	A	13: 100,356,338 (GRCm39)	E1092D	probably benign	Het
Npy6r	T	C	18: 44,408,759 (GRCm39)	I60T	probably damaging	Het
Or12j3	T	C	7: 139,952,930 (GRCm39)	T198A	probably benign	Het
Or13p3	A	T	4: 118,567,353 (GRCm39)	M250L	probably benign	Het
Or4f57	G	T	2: 111,790,872 (GRCm39)	P182H	probably damaging	Het
Or5aq6	A	T	2: 86,923,390 (GRCm39)	M117K	possibly damaging	Het
Or5k14	C	T	16: 58,692,976 (GRCm39)	C179Y	probably damaging	Het
Or6c215	G	A	10: 129,637,540 (GRCm39)	P285S	probably damaging	Het
Ovgp1	CCACTGGTGTTTCTAAGACCACCACTGGCATTTCTAAGACCATCACTGGTGTTTCTAAGACCACCACTGGCATTTCTAAGACCACCACTGGCATTTCTAAGACCACCACTGGGGTTTCTAAGATCACCACTGGTGTTTCTAAGACCACCACTGGCATTTCTAAGACCA	CCACTGGTGTTTCTAAGACCACCACTGGCATTTCTAAGACCACCACTGGCATTTCTAAGACCACCACTGGGGTTTCTAAGATCACCACTGGTGTTTCTAAGACCACCACTGGCATTTCTAAGACCA	3: 105,893,841 (GRCm39)		probably benign	Het
Oxtr	C	A	6: 112,466,310 (GRCm39)	R150L	probably damaging	Het
Pappa	A	T	4: 65,099,125 (GRCm39)	Y548F	probably damaging	Het
Pip4k2a	T	C	2: 18,912,890 (GRCm39)	K73R	probably damaging	Het
Pla2g4f	T	A	2: 120,132,781 (GRCm39)	D685V	probably damaging	Het
Ppp1r35	T	A	5: 137,777,577 (GRCm39)	L81Q	probably damaging	Het
Ptprf	C	A	4: 118,093,236 (GRCm39)	A469S	probably benign	Het
Ranbp3l	T	A	15: 9,057,304 (GRCm39)	N322K	probably damaging	Het
Rev3l	T	A	10: 39,698,967 (GRCm39)	Y1155N	possibly damaging	Het
Rfesd	T	C	13: 76,151,131 (GRCm39)	Y96C	probably damaging	Het
Runx1	C	T	16: 92,410,568 (GRCm39)	V283I	possibly damaging	Het
Sema5b	A	C	16: 35,466,092 (GRCm39)	Q125P	probably damaging	Het
Skic3	A	G	13: 76,275,158 (GRCm39)	D403G	probably benign	Het
Spef2	A	T	15: 9,725,307 (GRCm39)	M150K	probably damaging	Het
Srcap	T	G	7: 127,141,455 (GRCm39)	L1745W	probably damaging	Het
Srgap2	A	G	1: 131,283,365 (GRCm39)	Y239H	probably damaging	Het
Svil	T	A	18: 5,090,811 (GRCm39)	H1304Q	possibly damaging	Het
Syne1	T	C	10: 5,179,193 (GRCm39)	D4427G	possibly damaging	Het
Tg	T	A	15: 66,561,173 (GRCm39)	M1065K	probably benign	Het
Tgm2	T	C	2: 157,959,816 (GRCm39)	T656A	probably benign	Het
Ubr4	A	G	4: 139,153,235 (GRCm39)	K809E		Het
Vmn1r88	T	C	7: 12,912,546 (GRCm39)	S301P	probably damaging	Het
Wdr27	A	G	17: 15,111,980 (GRCm39)	V671A	possibly damaging	Het
Zbtb17	G	A	4: 141,193,131 (GRCm39)	V550I	possibly damaging	Het
Zeb2	A	T	2: 44,887,920 (GRCm39)	I379N	probably damaging	Het

Other mutations in Prss3b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01120:Prss3b	APN	6	41,008,607 (GRCm39)	missense	probably damaging	1.00
IGL01625:Prss3b	APN	6	41,009,882 (GRCm39)	missense	probably benign	0.01
IGL02159:Prss3b	APN	6	41,009,891 (GRCm39)	missense	probably benign	0.34
IGL03161:Prss3b	APN	6	41,011,240 (GRCm39)	missense	probably damaging	0.96
R0419:Prss3b	UTSW	6	41,011,281 (GRCm39)	missense	probably benign	0.05
R1715:Prss3b	UTSW	6	41,009,870 (GRCm39)	splice site	probably null
R2057:Prss3b	UTSW	6	41,009,315 (GRCm39)	missense	probably benign	0.06
R2059:Prss3b	UTSW	6	41,009,315 (GRCm39)	missense	probably benign	0.06
R2136:Prss3b	UTSW	6	41,012,396 (GRCm39)	missense	probably benign	0.28
R2446:Prss3b	UTSW	6	41,008,582 (GRCm39)	missense	probably benign	0.15
R4862:Prss3b	UTSW	6	41,009,345 (GRCm39)	missense	possibly damaging	0.74
R5068:Prss3b	UTSW	6	41,009,370 (GRCm39)	missense	probably benign	0.35
R5369:Prss3b	UTSW	6	41,009,940 (GRCm39)	missense	probably benign	0.00
R6392:Prss3b	UTSW	6	41,009,306 (GRCm39)	missense	probably damaging	1.00
R7010:Prss3b	UTSW	6	41,009,247 (GRCm39)	missense	probably benign	0.42
R7567:Prss3b	UTSW	6	41,010,127 (GRCm39)	missense	probably benign	0.04
R7727:Prss3b	UTSW	6	41,010,127 (GRCm39)	missense	probably benign	0.04
R7962:Prss3b	UTSW	6	41,012,387 (GRCm39)	missense	probably benign	0.01
R8373:Prss3b	UTSW	6	41,008,622 (GRCm39)	missense	possibly damaging	0.53
R8529:Prss3b	UTSW	6	41,009,369 (GRCm39)	missense	probably benign	0.00
R8883:Prss3b	UTSW	6	41,009,305 (GRCm39)	nonsense	probably null
R8916:Prss3b	UTSW	6	41,010,103 (GRCm39)	missense	probably damaging	1.00
R9066:Prss3b	UTSW	6	41,008,640 (GRCm39)	missense	probably benign	0.41
R9095:Prss3b	UTSW	6	41,010,038 (GRCm39)	missense	possibly damaging	0.76
R9180:Prss3b	UTSW	6	41,008,681 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCAGTGGTACTTACTGCCAGAG -3'
(R):5'- AAGGGAGTGCACACTTACCTAGG -3'

Sequencing Primer
(F):5'- TGGTACTTACTGCCAGAGCTCAG -3'
(R):5'- CCTAGGATATAAAATCACCTATGTG -3'

Posted On

2022-04-18