Mutagenetix > Incidental Mutation

Incidental Mutation 'R9392:Vmn2r25'

710621

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r25

Ensembl Gene

ENSMUSG00000094672

Gene Name

vomeronasal 2, receptor 25

Synonyms

EG545874

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R9392 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

123799773-123830149 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 123816937 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 215 (I215V)

Ref Sequence

ENSEMBL: ENSMUSP00000124342 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000162046]

AlphaFold

W4VSP2

Predicted Effect

probably benign
Transcript: ENSMUST00000162046
AA Change: I215V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000124342
Gene: ENSMUSG00000094672
AA Change: I215V

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	473	6e-31	PFAM
Pfam:NCD3G	519	572	5.8e-25	PFAM
Pfam:7tm_3	603	840	4.8e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (27/27)

Allele List at MGI

Other mutations in this stock

Total: 27 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd34c	A	G	9: 89,611,787 (GRCm39)	C185R	possibly damaging	Het
Apob	T	C	12: 8,057,098 (GRCm39)	V1860A	probably benign	Het
Bahcc1	C	T	11: 120,163,513 (GRCm39)	R604*	probably null	Het
Btbd16	C	T	7: 130,417,516 (GRCm39)	R344C	probably damaging	Het
Cdhr4	A	G	9: 107,873,507 (GRCm39)	D413G	probably damaging	Het
Col15a1	G	C	4: 47,288,200 (GRCm39)		probably benign	Het
Dchs1	T	A	7: 105,421,869 (GRCm39)	T184S	probably benign	Het
Dnah11	A	T	12: 118,011,055 (GRCm39)	Y2044*	probably null	Het
Dnah11	T	C	12: 118,141,290 (GRCm39)	T489A	probably benign	Het
Dnah7b	C	T	1: 46,162,898 (GRCm39)	Q415*	probably null	Het
Eml5	T	C	12: 98,867,199 (GRCm39)	Y30C	probably damaging	Het
Fat1	A	G	8: 45,476,228 (GRCm39)	Y1758C	probably damaging	Het
Fndc1	A	T	17: 7,991,957 (GRCm39)	S580T	unknown	Het
Ift57	T	C	16: 49,584,174 (GRCm39)	I394T	possibly damaging	Het
Itpr1	T	A	6: 108,390,837 (GRCm39)	S1579T	probably benign	Het
Kcnj14	A	T	7: 45,467,159 (GRCm39)	C396S	probably benign	Het
Lig3	G	A	11: 82,680,666 (GRCm39)	C413Y	probably benign	Het
Or8b38	T	C	9: 37,973,195 (GRCm39)	I193T	probably benign	Het
Pkd1l1	T	C	11: 8,794,567 (GRCm39)	T2315A		Het
Rptn	A	T	3: 93,305,721 (GRCm39)	H1018L	probably benign	Het
Rsph4a	G	A	10: 33,781,236 (GRCm39)	V29I	probably benign	Het
Shroom1	C	A	11: 53,354,674 (GRCm39)	P198Q	possibly damaging	Het
Sipa1l2	A	G	8: 126,194,960 (GRCm39)	V926A	probably benign	Het
Swap70	T	C	7: 109,865,191 (GRCm39)		probably null	Het
Tcerg1l	A	G	7: 137,815,164 (GRCm39)	V505A	probably damaging	Het
Ubr5	A	G	15: 37,984,251 (GRCm39)	S2299P		Het
Vmn2r65	T	A	7: 84,589,718 (GRCm39)	I733F	possibly damaging	Het

Other mutations in Vmn2r25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Vmn2r25	APN	6	123,830,130 (GRCm39)	missense	probably benign	0.25
IGL01781:Vmn2r25	APN	6	123,816,324 (GRCm39)	missense	possibly damaging	0.48
IGL01843:Vmn2r25	APN	6	123,829,962 (GRCm39)	missense	possibly damaging	0.67
IGL02023:Vmn2r25	APN	6	123,816,388 (GRCm39)	missense	probably damaging	0.96
IGL02502:Vmn2r25	APN	6	123,816,392 (GRCm39)	missense	probably damaging	0.96
IGL02709:Vmn2r25	APN	6	123,816,723 (GRCm39)	missense	possibly damaging	0.50
IGL03053:Vmn2r25	APN	6	123,800,077 (GRCm39)	missense	probably damaging	1.00
PIT4468001:Vmn2r25	UTSW	6	123,816,557 (GRCm39)	missense	probably benign	0.00
PIT4812001:Vmn2r25	UTSW	6	123,800,447 (GRCm39)	missense	probably damaging	1.00
R0054:Vmn2r25	UTSW	6	123,829,984 (GRCm39)	missense	probably benign	0.00
R0312:Vmn2r25	UTSW	6	123,805,539 (GRCm39)	splice site	probably benign
R0366:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R0390:Vmn2r25	UTSW	6	123,800,140 (GRCm39)	missense	probably damaging	1.00
R0466:Vmn2r25	UTSW	6	123,829,008 (GRCm39)	missense	probably benign	0.16
R0541:Vmn2r25	UTSW	6	123,816,786 (GRCm39)	missense	probably damaging	0.97
R0612:Vmn2r25	UTSW	6	123,816,481 (GRCm39)	missense	probably damaging	1.00
R0865:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R1219:Vmn2r25	UTSW	6	123,816,282 (GRCm39)	missense	probably benign	0.00
R1240:Vmn2r25	UTSW	6	123,828,864 (GRCm39)	missense	probably damaging	0.98
R1701:Vmn2r25	UTSW	6	123,828,754 (GRCm39)	splice site	probably null
R1780:Vmn2r25	UTSW	6	123,805,424 (GRCm39)	missense	probably damaging	1.00
R1809:Vmn2r25	UTSW	6	123,802,337 (GRCm39)	missense	probably benign	0.00
R1833:Vmn2r25	UTSW	6	123,816,643 (GRCm39)	missense	probably benign	0.01
R1964:Vmn2r25	UTSW	6	123,800,254 (GRCm39)	missense	possibly damaging	0.94
R2154:Vmn2r25	UTSW	6	123,816,805 (GRCm39)	missense	probably benign	0.01
R2164:Vmn2r25	UTSW	6	123,816,518 (GRCm39)	missense	possibly damaging	0.96
R3799:Vmn2r25	UTSW	6	123,830,143 (GRCm39)	missense	probably benign	0.12
R3836:Vmn2r25	UTSW	6	123,830,044 (GRCm39)	missense	probably damaging	1.00
R3946:Vmn2r25	UTSW	6	123,817,057 (GRCm39)	missense	probably damaging	0.97
R4282:Vmn2r25	UTSW	6	123,800,606 (GRCm39)	missense	probably damaging	1.00
R4367:Vmn2r25	UTSW	6	123,805,496 (GRCm39)	missense	probably damaging	1.00
R4438:Vmn2r25	UTSW	6	123,816,756 (GRCm39)	missense	probably benign	0.03
R4488:Vmn2r25	UTSW	6	123,799,819 (GRCm39)	missense	probably damaging	1.00
R4580:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.46
R4631:Vmn2r25	UTSW	6	123,829,962 (GRCm39)	missense	possibly damaging	0.94
R4765:Vmn2r25	UTSW	6	123,800,182 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r25	UTSW	6	123,805,406 (GRCm39)	missense	probably benign
R5207:Vmn2r25	UTSW	6	123,817,062 (GRCm39)	missense	probably damaging	1.00
R5254:Vmn2r25	UTSW	6	123,802,277 (GRCm39)	missense	probably damaging	1.00
R5444:Vmn2r25	UTSW	6	123,805,451 (GRCm39)	missense	probably benign	0.00
R5586:Vmn2r25	UTSW	6	123,802,255 (GRCm39)	missense	probably damaging	1.00
R5607:Vmn2r25	UTSW	6	123,805,318 (GRCm39)	missense	possibly damaging	0.49
R5985:Vmn2r25	UTSW	6	123,800,587 (GRCm39)	missense	probably benign
R6046:Vmn2r25	UTSW	6	123,799,876 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r25	UTSW	6	123,799,900 (GRCm39)	missense	possibly damaging	0.69
R6569:Vmn2r25	UTSW	6	123,828,941 (GRCm39)	missense	probably benign	0.01
R6826:Vmn2r25	UTSW	6	123,800,071 (GRCm39)	missense	probably damaging	1.00
R7054:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R7120:Vmn2r25	UTSW	6	123,805,394 (GRCm39)	missense	possibly damaging	0.51
R7177:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7287:Vmn2r25	UTSW	6	123,829,040 (GRCm39)	missense	possibly damaging	0.49
R7397:Vmn2r25	UTSW	6	123,800,498 (GRCm39)	missense	probably damaging	0.96
R7486:Vmn2r25	UTSW	6	123,800,101 (GRCm39)	missense	probably damaging	1.00
R7699:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7700:Vmn2r25	UTSW	6	123,816,882 (GRCm39)	missense	possibly damaging	0.94
R7759:Vmn2r25	UTSW	6	123,800,339 (GRCm39)	missense	probably damaging	0.99
R7802:Vmn2r25	UTSW	6	123,828,791 (GRCm39)	missense	possibly damaging	0.88
R7850:Vmn2r25	UTSW	6	123,805,431 (GRCm39)	missense	probably damaging	1.00
R8064:Vmn2r25	UTSW	6	123,800,581 (GRCm39)	nonsense	probably null
R8170:Vmn2r25	UTSW	6	123,829,976 (GRCm39)	missense	probably benign	0.09
R8340:Vmn2r25	UTSW	6	123,829,972 (GRCm39)	missense	probably benign	0.01
R8346:Vmn2r25	UTSW	6	123,802,350 (GRCm39)	missense	probably benign	0.00
R8395:Vmn2r25	UTSW	6	123,799,982 (GRCm39)	missense	possibly damaging	0.81
R8889:Vmn2r25	UTSW	6	123,800,569 (GRCm39)	missense	probably damaging	1.00
R9094:Vmn2r25	UTSW	6	123,805,391 (GRCm39)	missense	probably benign	0.44
R9204:Vmn2r25	UTSW	6	123,830,092 (GRCm39)	missense	probably benign	0.00
R9253:Vmn2r25	UTSW	6	123,816,960 (GRCm39)	missense	probably damaging	0.98
R9520:Vmn2r25	UTSW	6	123,830,066 (GRCm39)	nonsense	probably null
R9525:Vmn2r25	UTSW	6	123,800,164 (GRCm39)	missense	probably damaging	1.00
R9622:Vmn2r25	UTSW	6	123,816,579 (GRCm39)	missense	probably damaging	0.99
X0020:Vmn2r25	UTSW	6	123,816,359 (GRCm39)	missense	possibly damaging	0.95
Z1176:Vmn2r25	UTSW	6	123,799,856 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGATAACAGGTGTCAAAGTGAATCG -3'
(R):5'- TGTCCTAGAGTGACAAAACAGAGATC -3'

Sequencing Primer
(F):5'- CGTTCCATAAAACGTTTCCAGTTTAC -3'
(R):5'- AGATTCTTGGGACCAGAG -3'

Posted On

2022-04-18