Incidental Mutation 'R9397:Odr4'
| ID |
710869 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Odr4
|
| Ensembl Gene |
ENSMUSG00000006010 |
| Gene Name |
odr4 GPCR localization factor homolog |
| Synonyms |
BC003331, 1810053E15Rik |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.783)
|
| Stock # |
R9397 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
1 |
| Chromosomal Location |
150237056-150268831 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to T
at 150238616 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Alanine to Threonine
at position 434
(A434T)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000107544
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000006167]
[ENSMUST00000094477]
[ENSMUST00000097546]
[ENSMUST00000097547]
[ENSMUST00000111913]
|
| AlphaFold |
Q4PJX1 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000006167
AA Change: A412T
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000006167
Gene: ENSMUSG00000006010
AA Change: A412T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:ODR4-like
|
28 |
364 |
1.8e-107 |
PFAM |
|
transmembrane domain
|
402 |
424 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000094477
|
| SMART Domains |
Protein: ENSMUSP00000092050
Gene: ENSMUSG00000006010
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:ODR4-like
|
28 |
383 |
2.8e-120 |
PFAM |
|
transmembrane domain
|
424 |
446 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000097546
AA Change: A412T
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000095152
Gene: ENSMUSG00000006010
AA Change: A412T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:ODR4-like
|
28 |
364 |
1.8e-107 |
PFAM |
|
transmembrane domain
|
402 |
424 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000097547
AA Change: A402T
PolyPhen 2
Score 0.111 (Sensitivity: 0.93; Specificity: 0.86)
|
| SMART Domains |
Protein: ENSMUSP00000095153
Gene: ENSMUSG00000006010
AA Change: A402T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:ODR4-like
|
28 |
209 |
1.2e-57 |
PFAM |
|
Pfam:ODR4-like
|
206 |
354 |
3.1e-43 |
PFAM |
|
transmembrane domain
|
392 |
414 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000111913
AA Change: A434T
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000107544
Gene: ENSMUSG00000006010
AA Change: A434T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:ODR4-like
|
28 |
386 |
7.1e-113 |
PFAM |
|
transmembrane domain
|
424 |
446 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.8%
- 20x: 99.4%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 49 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930555G01Rik |
T |
G |
14: 5,051,639 (GRCm38) |
V66G |
possibly damaging |
Het |
| A630001G21Rik |
G |
T |
1: 85,653,002 (GRCm39) |
Y55* |
probably null |
Het |
| Abca8a |
G |
A |
11: 109,921,173 (GRCm39) |
T1474I |
probably damaging |
Het |
| Acaca |
T |
G |
11: 84,259,551 (GRCm39) |
I1962S |
probably damaging |
Het |
| Acap1 |
A |
T |
11: 69,775,672 (GRCm39) |
S455T |
probably damaging |
Het |
| Acbd7 |
G |
A |
2: 3,337,259 (GRCm39) |
|
probably null |
Het |
| Adamts9 |
A |
G |
6: 92,878,444 (GRCm39) |
L541P |
probably damaging |
Het |
| Ankrd49 |
T |
C |
9: 14,692,711 (GRCm39) |
N151S |
probably benign |
Het |
| Apcdd1 |
C |
T |
18: 63,055,731 (GRCm39) |
|
probably benign |
Het |
| Arhgap27 |
T |
C |
11: 103,231,115 (GRCm39) |
D58G |
probably damaging |
Het |
| Btbd18 |
G |
T |
2: 84,497,810 (GRCm39) |
A483S |
probably damaging |
Het |
| C2 |
A |
G |
17: 35,094,965 (GRCm39) |
S257P |
probably damaging |
Het |
| Catsper3 |
T |
A |
13: 55,946,725 (GRCm39) |
Y141N |
probably damaging |
Het |
| Cfap54 |
G |
A |
10: 92,833,147 (GRCm39) |
L1160F |
probably damaging |
Het |
| Chst13 |
G |
A |
6: 90,286,506 (GRCm39) |
P152L |
probably damaging |
Het |
| Cyp4f14 |
C |
T |
17: 33,130,516 (GRCm39) |
G141D |
probably damaging |
Het |
| Duox1 |
C |
T |
2: 122,150,783 (GRCm39) |
T286I |
possibly damaging |
Het |
| Epc2 |
G |
A |
2: 49,378,822 (GRCm39) |
G92E |
probably damaging |
Het |
| Fmnl3 |
C |
T |
15: 99,225,938 (GRCm39) |
|
probably null |
Het |
| Glyatl3 |
G |
A |
17: 41,223,565 (GRCm39) |
R59* |
probably null |
Het |
| Gm3633 |
T |
A |
14: 42,460,400 (GRCm39) |
Q181L |
|
Het |
| Guca1b |
C |
T |
17: 47,696,560 (GRCm39) |
T49M |
probably benign |
Het |
| Hdac9 |
T |
A |
12: 34,353,275 (GRCm39) |
H645L |
probably damaging |
Het |
| Hhip |
T |
C |
8: 80,719,112 (GRCm39) |
R472G |
probably benign |
Het |
| Ifi203 |
A |
G |
1: 173,765,547 (GRCm39) |
V9A |
probably damaging |
Het |
| Igkv9-123 |
A |
T |
6: 67,931,443 (GRCm39) |
V41D |
probably damaging |
Het |
| Il5ra |
T |
A |
6: 106,721,258 (GRCm39) |
T14S |
probably benign |
Het |
| Ints2 |
A |
G |
11: 86,135,311 (GRCm39) |
S368P |
probably benign |
Het |
| Kat6b |
T |
A |
14: 21,675,244 (GRCm39) |
S470T |
possibly damaging |
Het |
| Kmt2d |
A |
G |
15: 98,747,994 (GRCm39) |
V3110A |
unknown |
Het |
| Lama2 |
T |
C |
10: 26,981,117 (GRCm39) |
N1846D |
probably benign |
Het |
| Lrp1b |
T |
A |
2: 40,640,922 (GRCm39) |
E3507D |
|
Het |
| Ly6g6c |
A |
G |
17: 35,288,317 (GRCm39) |
N63S |
probably benign |
Het |
| Naprt |
C |
T |
15: 75,763,859 (GRCm39) |
V354I |
probably null |
Het |
| Nars1 |
A |
G |
18: 64,642,327 (GRCm39) |
S172P |
probably benign |
Het |
| Or6c5b |
A |
G |
10: 129,245,909 (GRCm39) |
R225G |
possibly damaging |
Het |
| Or7e177 |
T |
C |
9: 20,211,748 (GRCm39) |
I85T |
possibly damaging |
Het |
| Pcdhb6 |
A |
G |
18: 37,469,353 (GRCm39) |
D758G |
probably benign |
Het |
| Pkp4 |
G |
A |
2: 59,148,856 (GRCm39) |
W536* |
probably null |
Het |
| Polr3b |
C |
T |
10: 84,467,653 (GRCm39) |
L78F |
possibly damaging |
Het |
| Rasa4 |
T |
A |
5: 136,129,836 (GRCm39) |
V272E |
possibly damaging |
Het |
| Syne2 |
A |
G |
12: 76,040,849 (GRCm39) |
T3836A |
possibly damaging |
Het |
| Tbpl2 |
C |
T |
2: 23,966,070 (GRCm39) |
V308I |
possibly damaging |
Het |
| Tcea1 |
T |
A |
1: 4,962,141 (GRCm39) |
I176K |
probably damaging |
Het |
| Try5 |
A |
T |
6: 41,289,314 (GRCm39) |
F87L |
probably benign |
Het |
| Ttc8 |
G |
T |
12: 98,942,692 (GRCm39) |
G411* |
probably null |
Het |
| Vmn2r115 |
G |
A |
17: 23,564,152 (GRCm39) |
W108* |
probably null |
Het |
| Zdhhc13 |
C |
T |
7: 48,476,628 (GRCm39) |
T581I |
probably benign |
Het |
| Zfp777 |
G |
T |
6: 48,021,190 (GRCm39) |
T144K |
probably benign |
Het |
|
| Other mutations in Odr4 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01876:Odr4
|
APN |
1 |
150,258,089 (GRCm39) |
missense |
probably benign |
0.10 |
|
IGL02189:Odr4
|
APN |
1 |
150,247,784 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
IGL02257:Odr4
|
APN |
1 |
150,262,155 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02485:Odr4
|
APN |
1 |
150,239,240 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL02585:Odr4
|
APN |
1 |
150,239,272 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL02712:Odr4
|
APN |
1 |
150,262,107 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL02902:Odr4
|
APN |
1 |
150,260,179 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL03014:Odr4
|
APN |
1 |
150,258,804 (GRCm39) |
splice site |
probably benign |
|
|
IGL03124:Odr4
|
APN |
1 |
150,262,176 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL03181:Odr4
|
APN |
1 |
150,239,290 (GRCm39) |
missense |
probably benign |
0.06 |
|
IGL03344:Odr4
|
APN |
1 |
150,239,295 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1170:Odr4
|
UTSW |
1 |
150,262,142 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1796:Odr4
|
UTSW |
1 |
150,251,305 (GRCm39) |
missense |
probably benign |
|
|
R1902:Odr4
|
UTSW |
1 |
150,264,360 (GRCm39) |
splice site |
probably null |
|
|
R2149:Odr4
|
UTSW |
1 |
150,264,310 (GRCm39) |
missense |
probably benign |
0.05 |
|
R2155:Odr4
|
UTSW |
1 |
150,258,086 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R2375:Odr4
|
UTSW |
1 |
150,265,985 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3786:Odr4
|
UTSW |
1 |
150,260,282 (GRCm39) |
missense |
probably benign |
0.21 |
|
R3948:Odr4
|
UTSW |
1 |
150,264,308 (GRCm39) |
nonsense |
probably null |
|
|
R4589:Odr4
|
UTSW |
1 |
150,260,238 (GRCm39) |
missense |
probably benign |
0.11 |
|
R4590:Odr4
|
UTSW |
1 |
150,262,103 (GRCm39) |
splice site |
probably null |
|
|
R4815:Odr4
|
UTSW |
1 |
150,250,597 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5196:Odr4
|
UTSW |
1 |
150,258,140 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5437:Odr4
|
UTSW |
1 |
150,239,269 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5549:Odr4
|
UTSW |
1 |
150,247,909 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R5677:Odr4
|
UTSW |
1 |
150,250,588 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5896:Odr4
|
UTSW |
1 |
150,256,111 (GRCm39) |
missense |
probably benign |
0.10 |
|
R6472:Odr4
|
UTSW |
1 |
150,257,273 (GRCm39) |
missense |
probably benign |
0.15 |
|
R7108:Odr4
|
UTSW |
1 |
150,258,041 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7402:Odr4
|
UTSW |
1 |
150,262,107 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7662:Odr4
|
UTSW |
1 |
150,258,045 (GRCm39) |
missense |
probably benign |
|
|
R7767:Odr4
|
UTSW |
1 |
150,247,788 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7810:Odr4
|
UTSW |
1 |
150,268,659 (GRCm39) |
utr 5 prime |
probably benign |
|
|
R7916:Odr4
|
UTSW |
1 |
150,260,249 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8114:Odr4
|
UTSW |
1 |
150,264,308 (GRCm39) |
nonsense |
probably null |
|
|
R8120:Odr4
|
UTSW |
1 |
150,260,177 (GRCm39) |
splice site |
probably null |
|
|
R8435:Odr4
|
UTSW |
1 |
150,258,020 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
| Predicted Primers |
PCR Primer
(F):5'- GCTAAACAAGGCAGCTGGTG -3'
(R):5'- CCACCACTGAATTTTAGTCTGTAC -3'
Sequencing Primer
(F):5'- CTAAACAAGGCAGCTGGTGGTTTC -3'
(R):5'- AGTCTGTACATATCCATATCTGTCC -3'
|
| Posted On |
2022-04-18 |
Incidental Mutation