Incidental Mutation 'R9399:Midn'
ID 711010
Institutional Source Beutler Lab
Gene Symbol Midn
Ensembl Gene ENSMUSG00000035621
Gene Name midnolin
Synonyms 3000003C15Rik
MMRRC Submission
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R9399 (G1)
Quality Score 225.009
Status Not validated
Chromosome 10
Chromosomal Location 79984106-79994202 bp(+) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) C to T at 79992210 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Stop codon at position 421 (R421*)
Ref Sequence ENSEMBL: ENSMUSP00000046967 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000042057] [ENSMUST00000099492] [ENSMUST00000144526] [ENSMUST00000146516] [ENSMUST00000153477]
AlphaFold Q3TPJ7
Predicted Effect probably null
Transcript: ENSMUST00000042057
AA Change: R421*
SMART Domains Protein: ENSMUSP00000046967
Gene: ENSMUSG00000035621
AA Change: R421*

DomainStartEndE-ValueType
UBQ 32 102 3.39e-7 SMART
low complexity region 130 143 N/A INTRINSIC
low complexity region 197 211 N/A INTRINSIC
low complexity region 238 262 N/A INTRINSIC
low complexity region 283 302 N/A INTRINSIC
low complexity region 398 409 N/A INTRINSIC
low complexity region 434 453 N/A INTRINSIC
low complexity region 465 485 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000099492
AA Change: R378*
SMART Domains Protein: ENSMUSP00000097091
Gene: ENSMUSG00000035621
AA Change: R378*

DomainStartEndE-ValueType
UBQ 32 102 3.39e-7 SMART
low complexity region 154 168 N/A INTRINSIC
low complexity region 195 219 N/A INTRINSIC
low complexity region 240 259 N/A INTRINSIC
low complexity region 355 366 N/A INTRINSIC
low complexity region 391 410 N/A INTRINSIC
low complexity region 422 442 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000144526
SMART Domains Protein: ENSMUSP00000120988
Gene: ENSMUSG00000035621

DomainStartEndE-ValueType
low complexity region 23 36 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000146516
SMART Domains Protein: ENSMUSP00000119962
Gene: ENSMUSG00000035621

DomainStartEndE-ValueType
low complexity region 47 61 N/A INTRINSIC
low complexity region 88 112 N/A INTRINSIC
low complexity region 133 152 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000153477
SMART Domains Protein: ENSMUSP00000119787
Gene: ENSMUSG00000035621

DomainStartEndE-ValueType
low complexity region 23 36 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.9%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a protein that contains an ubiquitin-like domain. This protein may be involved in the regulation of brain development as inferred by its high expression level in the embryonic midbrain. This protein has been found to negatively regulate glucokinase activity and insulin secretion in pancreatic beta cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit cleft palate and small ovary. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3425401B19Rik A C 14: 32,384,615 (GRCm39) L450R probably damaging Het
4933427I04Rik T A 4: 123,754,413 (GRCm39) L109* probably null Het
Amotl2 T C 9: 102,606,531 (GRCm39) L576P probably damaging Het
Ankrd11 T C 8: 123,618,179 (GRCm39) N1891S probably benign Het
Arap2 T A 5: 62,763,455 (GRCm39) H1563L possibly damaging Het
Astn2 T C 4: 65,664,588 (GRCm39) T732A possibly damaging Het
Atp2b2 A G 6: 113,780,713 (GRCm39) I312T probably benign Het
Bcl9 C T 3: 97,113,289 (GRCm39) M1055I probably benign Het
Carm1 A G 9: 21,486,791 (GRCm39) N180D possibly damaging Het
Cd180 A T 13: 102,842,021 (GRCm39) T356S probably benign Het
Cdh18 A C 15: 23,173,899 (GRCm39) T38P probably damaging Het
Cdk7 G T 13: 100,840,988 (GRCm39) P308Q probably damaging Het
Chd5 C T 4: 152,468,592 (GRCm39) A1715V probably benign Het
Clptm1 A T 7: 19,367,842 (GRCm39) V590D probably damaging Het
Col18a1 C T 10: 76,916,584 (GRCm39) G364R unknown Het
Ddhd1 C T 14: 45,895,117 (GRCm39) G118R possibly damaging Het
Fgfr4 C T 13: 55,304,293 (GRCm39) T111M probably damaging Het
Fndc8 A T 11: 82,788,739 (GRCm39) T190S probably damaging Het
Foxp1 TTGCTGCTGCTGCTGCTGCTGTTGCTGCTGCTGCTGTTGTTGCTGCTGCTGTTGCTGCTGTTGCTGCTGCTGCTGCTGCTGCTGTTGCTGCTGCTGCTGTTGCTGCTGCTG TTGCTGCTGCTGCTGCTGTTGCTGCTGCTGCTGTTGTTGCTGCTGCTGTTGCTGCTGTTGCTGCTGCTGCTGCTGCTGCTGTTGCTGCTGCTGCTGTTGCTGCTGCTG 6: 99,052,866 (GRCm39) probably benign Het
Glra3 T A 8: 56,542,079 (GRCm39) I274N probably damaging Het
Gpsm2 G A 3: 108,590,090 (GRCm39) R499* probably null Het
Gzme T A 14: 56,355,796 (GRCm39) D172V probably damaging Het
Ift88 T A 14: 57,717,385 (GRCm39) D536E probably benign Het
Itih3 T C 14: 30,643,335 (GRCm39) T151A probably benign Het
Kctd7 T C 5: 130,177,033 (GRCm39) Y95H probably damaging Het
Lipe A T 7: 25,097,227 (GRCm39) C239S probably benign Het
Lpcat3 T C 6: 124,640,283 (GRCm39) S38P probably benign Het
Mical2 C T 7: 111,946,082 (GRCm39) R1015C probably damaging Het
Mmp17 A T 5: 129,671,686 (GRCm39) K79* probably null Het
Or8b39 A G 9: 37,997,020 (GRCm39) H296R probably benign Het
Or8k16 G C 2: 85,520,395 (GRCm39) L207F probably damaging Het
Pla2r1 A G 2: 60,282,744 (GRCm39) probably null Het
Ppp1r3a A G 6: 14,755,010 (GRCm39) V79A probably damaging Het
Prkd3 G T 17: 79,264,719 (GRCm39) P633H probably damaging Het
Prss40 T A 1: 34,591,794 (GRCm39) S294C probably damaging Het
Raph1 T A 1: 60,565,154 (GRCm39) Q111L probably benign Het
Rufy3 A T 5: 88,797,725 (GRCm39) N634I possibly damaging Het
Sel1l3 A C 5: 53,265,486 (GRCm39) V1102G probably benign Het
Sh2b1 AGCTCAGCC AGCTCAGCCCCGGGGACCCGCTCAGCC 7: 126,066,750 (GRCm39) probably benign Het
Sh2b1 GG GGCACCAGCTCAGCCCCGCG 7: 126,066,762 (GRCm39) probably benign Het
Sh2b1 TGGGGACCAGCTCAGCCACGGGGACCAGCTC TGGGGACCAGCTCAGCCACGGGGACCAGCTCAGCCACGGGGACCAGCTC 7: 126,066,742 (GRCm39) probably benign Het
Sh2b1 GGGACCAGCTCAGCCACG GGGACCAGCTCAGCCACGTGGACCAGCTCAGCCACG 7: 126,066,744 (GRCm39) probably benign Het
Slc25a23 C A 17: 57,360,930 (GRCm39) G165C probably damaging Het
Spata31e1 T A 13: 49,940,175 (GRCm39) T512S possibly damaging Het
Sytl2 T A 7: 90,041,658 (GRCm39) D585E probably benign Het
Ttc14 A G 3: 33,858,856 (GRCm39) T372A possibly damaging Het
Vmn1r43 G A 6: 89,846,877 (GRCm39) T203M probably damaging Het
Vmn2r82 T A 10: 79,214,768 (GRCm39) Y250* probably null Het
Vmn2r92 T A 17: 18,389,137 (GRCm39) S484T probably benign Het
Other mutations in Midn
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01586:Midn APN 10 79,992,477 (GRCm39) unclassified probably benign
IGL01969:Midn APN 10 79,991,093 (GRCm39) missense probably benign 0.00
IGL02824:Midn APN 10 79,989,486 (GRCm39) missense possibly damaging 0.91
Dunkel UTSW 10 79,989,918 (GRCm39) missense probably damaging 0.96
full_moon UTSW 10 79,985,946 (GRCm39) missense possibly damaging 0.66
Midnight UTSW 10 79,990,291 (GRCm39) missense probably damaging 0.98
Sepia UTSW 10 79,987,238 (GRCm39) missense probably null 0.26
R0684:Midn UTSW 10 79,992,336 (GRCm39) missense probably damaging 1.00
R1517:Midn UTSW 10 79,989,957 (GRCm39) missense probably damaging 0.96
R1926:Midn UTSW 10 79,987,495 (GRCm39) missense probably damaging 1.00
R2004:Midn UTSW 10 79,990,983 (GRCm39) missense probably benign 0.13
R2016:Midn UTSW 10 79,985,949 (GRCm39) missense possibly damaging 0.91
R2340:Midn UTSW 10 79,985,946 (GRCm39) missense possibly damaging 0.66
R2483:Midn UTSW 10 79,986,144 (GRCm39) missense probably benign 0.16
R3622:Midn UTSW 10 79,986,144 (GRCm39) missense probably benign 0.16
R3624:Midn UTSW 10 79,986,144 (GRCm39) missense probably benign 0.16
R4296:Midn UTSW 10 79,987,553 (GRCm39) missense probably damaging 1.00
R4740:Midn UTSW 10 79,987,238 (GRCm39) missense probably null 0.26
R4930:Midn UTSW 10 79,991,189 (GRCm39) missense probably benign
R4977:Midn UTSW 10 79,986,018 (GRCm39) missense probably damaging 1.00
R5423:Midn UTSW 10 79,991,027 (GRCm39) missense probably benign 0.15
R6149:Midn UTSW 10 79,990,291 (GRCm39) missense probably damaging 0.98
R6542:Midn UTSW 10 79,992,418 (GRCm39) missense probably damaging 0.97
R6826:Midn UTSW 10 79,989,961 (GRCm39) nonsense probably null
R7478:Midn UTSW 10 79,991,156 (GRCm39) missense possibly damaging 0.53
R8025:Midn UTSW 10 79,991,126 (GRCm39) missense probably benign 0.00
R8819:Midn UTSW 10 79,990,234 (GRCm39) missense probably damaging 1.00
R8820:Midn UTSW 10 79,990,234 (GRCm39) missense probably damaging 1.00
R8870:Midn UTSW 10 79,985,939 (GRCm39) missense probably damaging 0.96
R9040:Midn UTSW 10 79,989,918 (GRCm39) missense probably damaging 0.96
R9228:Midn UTSW 10 79,990,275 (GRCm39) missense probably damaging 1.00
R9784:Midn UTSW 10 79,992,247 (GRCm39) missense probably damaging 1.00
X0018:Midn UTSW 10 79,989,831 (GRCm39) missense possibly damaging 0.90
Z1176:Midn UTSW 10 79,989,462 (GRCm39) missense probably benign
Z1177:Midn UTSW 10 79,986,074 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TGTGCAACACTTACTCAGCCC -3'
(R):5'- TCCAAACGGAGTCCTCGAAG -3'

Sequencing Primer
(F):5'- CACAGAACTGCCTGCTCTC -3'
(R):5'- TCGAAGTCGAGGCCCAAG -3'
Posted On 2022-04-18