Mutagenetix > Incidental Mutation

Incidental Mutation 'R9402:Gbp10'

711278

Institutional Source

Beutler Lab

Gene Symbol

Gbp10

Ensembl Gene

ENSMUSG00000105096

Gene Name

guanylate-binding protein 10

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R9402 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

105215699-105239533 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 105233997 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 96 (T96S)

Ref Sequence

ENSEMBL: ENSMUSP00000069042 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050011] [ENSMUST00000065588]

AlphaFold

Q000W5

Predicted Effect

probably benign
Transcript: ENSMUST00000050011

SMART Domains

Protein: ENSMUSP00000062528
Gene: ENSMUSG00000079362

Domain	Start	End	E-Value	Type
Pfam:GBP	16	279	7.6e-118	PFAM
Pfam:GBP_C	281	575	2.1e-117	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000065588
AA Change: T96S

PolyPhen 2 Score 0.824 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000069042
Gene: ENSMUSG00000105096
AA Change: T96S

Domain	Start	End	E-Value	Type
Pfam:GBP	16	279	5e-115	PFAM
Pfam:GBP_C	281	575	3.6e-117	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5730559C18Rik	T	G	1: 136,227,610	S86R	probably benign	Het
Abca9	T	A	11: 110,158,328	Q218L	probably benign	Het
Atg2b	A	G	12: 105,648,423	F1083S	probably damaging	Het
Bche	T	C	3: 73,701,323	N257D	probably benign	Het
Cfap221	T	A	1: 119,932,821	M692L	probably benign	Het
Cfap46	G	T	7: 139,635,949	P1530Q	unknown	Het
Crim1	CCGC	CC	17: 78,350,865		probably null	Het
Cyp4f39	T	C	17: 32,491,209		probably null	Het
Daam1	A	G	12: 71,959,830	T674A	probably benign	Het
Elp2	A	T	18: 24,626,163	I526L	probably benign	Het
Exoc4	T	A	6: 33,476,143	*523K	probably null	Het
Fbxl7	G	A	15: 26,552,503	S226L	probably damaging	Het
Fry	G	A	5: 150,433,696	E1903K	possibly damaging	Het
Fry	G	A	5: 150,436,853	R1988K	probably damaging	Het
Gas1	A	T	13: 60,176,202	M206K	probably damaging	Het
Gm14569	G	A	X: 36,430,896	P1387S	probably damaging	Het
Gpaa1	A	G	15: 76,332,218	T105A	probably benign	Het
Hoxc13	C	A	15: 102,921,616	C143*	probably null	Het
Htt	T	C	5: 34,848,980	I1411T	probably damaging	Het
Jmy	C	T	13: 93,499,170	C46Y	probably damaging	Het
Klhl40	A	G	9: 121,780,416	Y453C	possibly damaging	Het
Krit1	T	A	5: 3,822,210	Y460N	possibly damaging	Het
Lamb3	C	A	1: 193,331,396	T526K		Het
Lrrc29	T	A	8: 105,323,356	Y12F	probably benign	Het
Luzp1	T	A	4: 136,543,182	H905Q	probably damaging	Het
Lyst	T	A	13: 13,637,878	H958Q	probably benign	Het
Magi1	T	C	6: 94,283,297	N9S	probably benign	Het
Mmadhc	A	T	2: 50,281,107	V231E	probably benign	Het
Muc5b	T	A	7: 141,845,414	M364K	unknown	Het
Nae1	A	G	8: 104,528,185		probably null	Het
Ndst4	C	T	3: 125,724,736	S354L	probably benign	Het
Nlgn2	A	G	11: 69,828,107	F252S		Het
Nlrp9a	A	T	7: 26,570,605	N874I	possibly damaging	Het
Nodal	T	C	10: 61,423,600	I272T	probably damaging	Het
Olfr1234	C	A	2: 89,362,779	G217*	probably null	Het
Olfr1259	A	T	2: 89,943,940	Y58*	probably null	Het
Olfr599	T	C	7: 103,338,989	S312P	probably benign	Het
Olfr645	G	A	7: 104,084,403	R226*	probably null	Het
Orm1	C	A	4: 63,345,132	Q61K	possibly damaging	Het
Pcdh17	T	C	14: 84,447,206	V371A	probably damaging	Het
Pcdhb19	C	T	18: 37,499,479	Q776*	probably null	Het
Phf14	A	G	6: 11,933,780	R214G	possibly damaging	Het
Phf3	G	A	1: 30,811,847	T1142M	probably damaging	Het
Pigz	A	G	16: 31,945,369	E415G	probably damaging	Het
Pramel5	T	C	4: 144,271,456	T406A	probably benign	Het
Prtg	A	G	9: 72,911,971	E1082G	probably benign	Het
Rtp3	A	G	9: 110,985,963	C445R	unknown	Het
Scn3b	A	T	9: 40,282,556	E193V	probably damaging	Het
Scn7a	A	T	2: 66,680,112	N1315K	probably damaging	Het
Snx14	C	T	9: 88,407,437	G254D	probably damaging	Het
Srbd1	T	C	17: 86,099,277	D560G	probably benign	Het
Srp72	T	A	5: 76,976,482	L65*	probably null	Het
Steap1	T	A	5: 5,740,664	I95F		Het
Taf3	A	G	2: 9,951,112		probably null	Het
Taok2	T	C	7: 126,870,228	N1143D		Het
Tas2r114	C	T	6: 131,689,931	G45S	possibly damaging	Het
Tcerg1l	T	C	7: 138,209,822	K548E	probably damaging	Het
Tcp1	T	A	17: 12,922,618	L328Q	probably benign	Het
Tns2	A	G	15: 102,113,188	H1096R	probably damaging	Het
Trps1	A	T	15: 50,846,256	Y233N	probably damaging	Het
Tubgcp6	A	G	15: 89,102,861	V1303A	probably benign	Het
Ugt1a2	T	C	1: 88,200,962	F109S	possibly damaging	Het
Vmn1r43	G	A	6: 89,869,895	T203M	probably damaging	Het
Zfp748	A	G	13: 67,545,392	V54A	probably benign	Het

Other mutations in Gbp10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01470:Gbp10	APN	5	105221114	splice site	probably benign
IGL01680:Gbp10	APN	5	105224271	splice site	probably null
IGL01809:Gbp10	APN	5	105217359	missense	probably benign
IGL01845:Gbp10	APN	5	105219949	splice site	probably null
IGL02011:Gbp10	APN	5	105221101	missense	probably damaging	1.00
IGL02063:Gbp10	APN	5	105236040	missense	possibly damaging	0.95
IGL02533:Gbp10	APN	5	105220035	missense	probably damaging	1.00
IGL02612:Gbp10	APN	5	105218502	missense	possibly damaging	0.88
R0349:Gbp10	UTSW	5	105221076	missense	possibly damaging	0.60
R0462:Gbp10	UTSW	5	105218524	missense	possibly damaging	0.67
R0535:Gbp10	UTSW	5	105221011	missense	possibly damaging	0.95
R1223:Gbp10	UTSW	5	105219001	missense	probably damaging	1.00
R1704:Gbp10	UTSW	5	105224351	missense	probably damaging	1.00
R1792:Gbp10	UTSW	5	105224300	missense	probably damaging	1.00
R3738:Gbp10	UTSW	5	105224458	missense	possibly damaging	0.84
R3739:Gbp10	UTSW	5	105224458	missense	possibly damaging	0.84
R4035:Gbp10	UTSW	5	105224458	missense	possibly damaging	0.84
R4421:Gbp10	UTSW	5	105224651	splice site	probably null
R5207:Gbp10	UTSW	5	105224709	missense	probably benign	0.05
R5338:Gbp10	UTSW	5	105224300	missense	probably damaging	1.00
R6010:Gbp10	UTSW	5	105224339	missense	probably damaging	1.00
R6045:Gbp10	UTSW	5	105218403	missense	probably damaging	0.98
R6156:Gbp10	UTSW	5	105236149	start gained	probably benign
R6285:Gbp10	UTSW	5	105218460	missense	probably damaging	1.00
R6525:Gbp10	UTSW	5	105236084	missense	probably benign	0.01
R6908:Gbp10	UTSW	5	105221032	missense	probably damaging	0.97
R7339:Gbp10	UTSW	5	105220098	missense	possibly damaging	0.77
R7396:Gbp10	UTSW	5	105236149	start gained	probably benign
R7397:Gbp10	UTSW	5	105236149	start gained	probably benign
R7399:Gbp10	UTSW	5	105236149	start gained	probably benign
R7554:Gbp10	UTSW	5	105236149	start gained	probably benign
R7555:Gbp10	UTSW	5	105236149	start gained	probably benign
R7574:Gbp10	UTSW	5	105236149	start gained	probably benign
R7575:Gbp10	UTSW	5	105236149	start gained	probably benign
R7576:Gbp10	UTSW	5	105236149	start gained	probably benign
R7577:Gbp10	UTSW	5	105236149	start gained	probably benign
R7578:Gbp10	UTSW	5	105236149	start gained	probably benign
R7975:Gbp10	UTSW	5	105221101	missense	probably benign	0.20
R8293:Gbp10	UTSW	5	105224369	missense	probably damaging	1.00
R8708:Gbp10	UTSW	5	105220965	missense	probably damaging	1.00
R9002:Gbp10	UTSW	5	105221981	missense	probably benign	0.00
R9086:Gbp10	UTSW	5	105218503	missense	probably benign	0.00
R9149:Gbp10	UTSW	5	105218995	missense	probably damaging	0.98
R9582:Gbp10	UTSW	5	105224390	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- TGGAACCCAGGAATTTTAACTGTTG -3'
(R):5'- TTTAGCACACAAGGCAGCCTG -3'

Sequencing Primer
(F):5'- GCATGTTTTGCCTATTAAGATACAC -3'
(R):5'- GTGCAGGACACTCACATCC -3'

Posted On

2022-05-16