Mutagenetix > Incidental Mutation

Incidental Mutation 'R9407:Or14a259'

711593

Institutional Source

Beutler Lab

Gene Symbol

Or14a259

Ensembl Gene

ENSMUSG00000055571

Gene Name

olfactory receptor family 14 subfamily A member 259

Synonyms

GA_x6K02T2NHDJ-9744055-9745014, Olfr305, MOR219-2

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.121) question?

Stock #

R9407 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

86012584-86013543 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to C at 86013194 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Stop codon at position 117 (S117*)

Ref Sequence

ENSEMBL: ENSMUSP00000149762 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069236] [ENSMUST00000213255] [ENSMUST00000213869] [ENSMUST00000216700]

AlphaFold

Q7TS02

Predicted Effect

probably null
Transcript: ENSMUST00000069236
AA Change: S117*

SMART Domains

Protein: ENSMUSP00000068650
Gene: ENSMUSG00000055571
AA Change: S117*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	2.3e-40	PFAM
Pfam:7tm_1	39	288	3.3e-21	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213255
AA Change: S117*

Predicted Effect

probably null
Transcript: ENSMUST00000213869
AA Change: S117*

Predicted Effect

probably null
Transcript: ENSMUST00000216700
AA Change: S117*

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930578I06Rik	T	C	14: 64,223,629 (GRCm39)	Y49C	probably damaging	Het
Abca6	A	T	11: 110,093,210 (GRCm39)	L1021*	probably null	Het
Abcc9	A	G	6: 142,574,229 (GRCm39)	S1003P	possibly damaging	Het
Aldh4a1	A	G	4: 139,372,345 (GRCm39)	I505V	probably benign	Het
Brwd1	T	C	16: 95,803,693 (GRCm39)	D2159G	possibly damaging	Het
C1s2	T	C	6: 124,602,454 (GRCm39)	I586V	probably benign	Het
Cd300e	C	A	11: 114,946,171 (GRCm39)	D97Y	probably damaging	Het
Celsr3	A	G	9: 108,723,596 (GRCm39)	D2877G	probably damaging	Het
Cep41	T	C	6: 30,655,841 (GRCm39)	N328D	probably benign	Het
Cldn15	G	A	5: 137,003,765 (GRCm39)	V227M	probably damaging	Het
Clec10a	T	G	11: 70,060,155 (GRCm39)	I98S	probably damaging	Het
Cpne4	A	G	9: 104,749,963 (GRCm39)	Q89R	probably damaging	Het
Cryba2	C	A	1: 74,932,037 (GRCm39)	A4S	probably benign	Het
Csmd2	G	A	4: 128,442,613 (GRCm39)	A3222T		Het
Dapk1	C	A	13: 60,898,991 (GRCm39)	Y820*	probably null	Het
Efcab5	G	A	11: 77,022,934 (GRCm39)	T593I	probably damaging	Het
Entrep3	C	A	3: 89,094,645 (GRCm39)	A416D	possibly damaging	Het
Gar1	A	G	3: 129,620,608 (GRCm39)	Y160H	probably damaging	Het
Gm21834	T	A	17: 58,048,863 (GRCm39)	I118L	probably benign	Het
Gtf2a1l	G	A	17: 89,001,531 (GRCm39)	G129D	probably damaging	Het
Gzmb	T	A	14: 56,497,712 (GRCm39)	Y176F	probably benign	Het
Helz2	T	G	2: 180,881,975 (GRCm39)	K273Q	probably benign	Het
Ifi207	T	A	1: 173,555,234 (GRCm39)	E816V	probably damaging	Het
Insr	A	C	8: 3,235,106 (GRCm39)	D694E	probably benign	Het
Insyn2b	G	A	11: 34,352,072 (GRCm39)	R38Q	probably damaging	Het
Ipcef1	A	T	10: 6,870,036 (GRCm39)	C172*	probably null	Het
Itgal	T	G	7: 126,921,796 (GRCm39)		probably null	Het
Laptm5	A	T	4: 130,655,990 (GRCm39)	M103L		Het
Lypd4	A	T	7: 24,566,160 (GRCm39)	C55S	probably null	Het
Mccc1	A	C	3: 36,030,865 (GRCm39)	H400Q	possibly damaging	Het
Myl4	C	T	11: 104,474,887 (GRCm39)	R79W	probably damaging	Het
Myo18a	A	G	11: 77,709,596 (GRCm39)	T569A	probably benign	Het
Or4c117	T	A	2: 88,955,629 (GRCm39)	T149S	probably benign	Het
Or5ak24	A	G	2: 85,261,060 (GRCm39)	S38P	probably damaging	Het
Otof	T	A	5: 30,538,265 (GRCm39)	D1153V	probably damaging	Het
Pcdha12	A	T	18: 37,153,614 (GRCm39)	D111V	probably damaging	Het
Pomt2	G	T	12: 87,157,146 (GRCm39)	F724L	probably damaging	Het
Ros1	T	A	10: 51,994,491 (GRCm39)	Y1284F	probably damaging	Het
Skint6	T	C	4: 113,034,224 (GRCm39)	K301R	possibly damaging	Het
Slc30a5	T	A	13: 100,951,214 (GRCm39)	R223*	probably null	Het
Slc35a1	A	T	4: 34,675,181 (GRCm39)	V119E	probably damaging	Het
Slc6a11	A	T	6: 114,220,914 (GRCm39)	I482F	probably damaging	Het
Tektl1	T	C	10: 78,583,128 (GRCm39)	T419A	probably damaging	Het
Tgm1	A	T	14: 55,942,991 (GRCm39)	C616*	probably null	Het
Tiam2	T	A	17: 3,553,298 (GRCm39)	I1125N	probably damaging	Het
Tln2	T	C	9: 67,136,732 (GRCm39)	D1251G	probably damaging	Het
Tnfaip2	C	G	12: 111,412,161 (GRCm39)	S187R	probably benign	Het
Ush2a	A	G	1: 188,644,045 (GRCm39)	Y4469C	probably damaging	Het
Vmn1r229	G	C	17: 21,035,259 (GRCm39)	C168S	probably damaging	Het
Vmn2r53	A	G	7: 12,335,124 (GRCm39)	S179P	probably damaging	Het
Zfp474	A	C	18: 52,771,502 (GRCm39)	I52L	probably benign	Het

Other mutations in Or14a259

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01071:Or14a259	APN	7	86,012,768 (GRCm39)	missense	possibly damaging	0.46
IGL02102:Or14a259	APN	7	86,013,074 (GRCm39)	missense	probably benign	0.25
IGL02424:Or14a259	APN	7	86,012,688 (GRCm39)	missense	probably benign	0.02
IGL02664:Or14a259	APN	7	86,012,811 (GRCm39)	missense	possibly damaging	0.94
IGL03167:Or14a259	APN	7	86,013,128 (GRCm39)	missense	probably damaging	1.00
R0035:Or14a259	UTSW	7	86,013,395 (GRCm39)	missense	possibly damaging	0.95
R0373:Or14a259	UTSW	7	86,013,013 (GRCm39)	nonsense	probably null
R0510:Or14a259	UTSW	7	86,013,035 (GRCm39)	missense	probably benign	0.21
R2214:Or14a259	UTSW	7	86,013,414 (GRCm39)	missense	probably benign	0.01
R3147:Or14a259	UTSW	7	86,013,092 (GRCm39)	missense	probably benign	0.01
R3623:Or14a259	UTSW	7	86,013,308 (GRCm39)	missense	probably benign	0.02
R4155:Or14a259	UTSW	7	86,013,270 (GRCm39)	missense	probably benign	0.00
R4332:Or14a259	UTSW	7	86,013,080 (GRCm39)	missense	probably benign	0.01
R4785:Or14a259	UTSW	7	86,012,943 (GRCm39)	missense	probably damaging	1.00
R4834:Or14a259	UTSW	7	86,012,952 (GRCm39)	missense	probably benign	0.21
R4871:Or14a259	UTSW	7	86,012,692 (GRCm39)	missense	probably damaging	1.00
R5161:Or14a259	UTSW	7	86,013,546 (GRCm39)	splice site	probably null
R5254:Or14a259	UTSW	7	86,013,398 (GRCm39)	missense	possibly damaging	0.82
R6430:Or14a259	UTSW	7	86,013,181 (GRCm39)	nonsense	probably null
R7734:Or14a259	UTSW	7	86,013,476 (GRCm39)	missense	not run
R8520:Or14a259	UTSW	7	86,013,471 (GRCm39)	missense	probably benign	0.00
R8682:Or14a259	UTSW	7	86,013,373 (GRCm39)	missense	probably damaging	0.99
R9728:Or14a259	UTSW	7	86,012,771 (GRCm39)	missense	probably damaging	1.00
RF010:Or14a259	UTSW	7	86,012,594 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TCATGAGTGATCCAGAGCAGG -3'
(R):5'- AACACCCATGTACTTCTTCTTGAAG -3'

Sequencing Primer
(F):5'- TCCAGAGCAGGAAATCCTTAAG -3'
(R):5'- CCATTTCCATACTTGGATGT -3'

Posted On

2022-05-16