Mutagenetix > Incidental Mutations

Incidental Mutation 'R9412:Dclk3'

711859

Institutional Source

Beutler Lab

Gene Symbol

Dclk3

Ensembl Gene

ENSMUSG00000032500

Gene Name

doublecortin-like kinase 3

Synonyms

Click-I, -II related, Dcamkl3

Accession Numbers

Genbank: NM_172928; MGI: 3039580

Is this an essential gene?

Possibly non essential (E-score: 0.304) question?

Stock #

R9412 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

111439081-111489118 bp(+) (GRCm38)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

G to A at 111482751 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000107510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111879]

AlphaFold

Q8BWQ5

Predicted Effect

probably null
Transcript: ENSMUST00000111879

SMART Domains

Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
Pfam:DCX	115	177	2.9e-17	PFAM
low complexity region	200	218	N/A	INTRINSIC
low complexity region	267	277	N/A	INTRINSIC
low complexity region	479	496	N/A	INTRINSIC
S_TKc	514	771	1.63e-110	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700029H14Rik	T	A	8: 13,554,695	T203S	possibly damaging	Het
2310035C23Rik	A	G	1: 105,734,563	T936A	probably benign	Het
2700049A03Rik	G	A	12: 71,188,683	E1136K	possibly damaging	Het
Abca6	C	A	11: 110,212,233	R844L	probably damaging	Het
Abcb9	C	T	5: 124,083,690	R207Q	probably benign	Het
Abcd4	T	C	12: 84,608,807	R343G	probably damaging	Het
Arhgap45	T	A	10: 80,019,730	M1K	probably null	Het
Art3	A	G	5: 92,393,154	Y252C	probably damaging	Het
Ascc3	T	C	10: 50,649,134	M475T	probably benign	Het
Atxn2	C	T	5: 121,802,138	P992L	possibly damaging	Het
Ddx21	T	C	10: 62,594,102	T288A	possibly damaging	Het
Fat3	T	C	9: 15,997,407	D2433G	probably damaging	Het
Flnc	G	A	6: 29,441,485	R422Q	probably benign	Het
Fmnl2	G	T	2: 53,117,004	R776L	unknown	Het
Fndc1	T	A	17: 7,772,366	T833S	unknown	Het
Igf1r	T	A	7: 68,207,253	Y988N	probably damaging	Het
Ints2	T	C	11: 86,226,763	Y711C	probably damaging	Het
Irx5	A	G	8: 92,359,723	K145E	probably damaging	Het
Kdm5a	A	G	6: 120,389,030	Y377C	probably damaging	Het
Lrp1	T	A	10: 127,573,418	T1611S	probably damaging	Het
Lrrc28	T	A	7: 67,531,764	E241V	probably damaging	Het
Med23	T	C	10: 24,902,121	F789S	probably damaging	Het
Ncapd3	C	T	9: 27,056,155	Q596*	probably null	Het
Nrde2	C	A	12: 100,130,422	E1040*	probably null	Het
Olfr136	A	T	17: 38,335,429	T91S	possibly damaging	Het
Pcdh15	CAGAGA	CAGA	10: 74,645,831		probably null	Het
Pcm1	T	G	8: 41,287,751	N972K	probably damaging	Het
Peg10	C	CTCT	6: 4,756,453		probably benign	Het
Pla2g4a	T	C	1: 149,880,021	T222A	probably damaging	Het
Ptchd3	A	T	11: 121,841,953	K556N	possibly damaging	Het
Scap	T	C	9: 110,378,605	C461R	possibly damaging	Het
Scgb1b10	T	A	7: 32,101,202	L72*	probably null	Het
Serpinb6c	C	A	13: 33,897,388	A93S	probably benign	Het
Serpinb9c	C	A	13: 33,150,248	L298F	probably damaging	Het
Slf2	A	T	19: 44,942,021	E512D	probably benign	Het
Snapc3	C	T	4: 83,436,333	H194Y	probably benign	Het
Suox	T	A	10: 128,671,889	D90V	possibly damaging	Het
Supt20	TCAGCAGCAGCAGCAGCAGCAGCA	TCAGCAGCAGCAGCAGCAGCA	3: 54,727,648		probably benign	Het
Tert	C	T	13: 73,648,927	R1095W	probably benign	Het
Unc13c	T	C	9: 73,932,490	I360V	probably benign	Het
Uqcc1	A	G	2: 155,851,409	C235R	probably benign	Het
Vmn2r9	T	A	5: 108,843,618	I626F	probably damaging	Het
Vmn2r90	T	G	17: 17,733,951	C792W	probably damaging	Het
Zcchc11	A	T	4: 108,557,364	H46L		Het
Zfp57	C	T	17: 37,009,922	P223S	probably benign	Het
Zfp787	T	C	7: 6,132,947	T102A	probably damaging	Het

Other mutations in Dclk3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Dclk3	APN	9	111467843	nonsense	probably null
IGL02125:Dclk3	APN	9	111469107	missense	probably damaging	1.00
IGL02547:Dclk3	APN	9	111469023	missense	probably damaging	1.00
IGL03393:Dclk3	APN	9	111488673	utr 3 prime	probably benign
G1citation:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
IGL02984:Dclk3	UTSW	9	111488575	missense	probably damaging	1.00
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0432:Dclk3	UTSW	9	111484935	missense	probably damaging	1.00
R0440:Dclk3	UTSW	9	111469163	missense	probably damaging	1.00
R0530:Dclk3	UTSW	9	111482721	missense	probably damaging	1.00
R1024:Dclk3	UTSW	9	111469070	missense	possibly damaging	0.95
R1443:Dclk3	UTSW	9	111469020	missense	probably benign	0.01
R1474:Dclk3	UTSW	9	111469236	missense	probably benign	0.43
R1479:Dclk3	UTSW	9	111468546	missense	probably benign
R1482:Dclk3	UTSW	9	111467820	missense	possibly damaging	0.90
R1543:Dclk3	UTSW	9	111468054	missense	probably benign	0.04
R1552:Dclk3	UTSW	9	111488579	missense	probably damaging	1.00
R1559:Dclk3	UTSW	9	111469208	missense	probably damaging	1.00
R2011:Dclk3	UTSW	9	111468354	missense	probably benign	0.00
R2369:Dclk3	UTSW	9	111488542	missense	probably benign	0.16
R4111:Dclk3	UTSW	9	111469080	missense	probably damaging	0.99
R4510:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4511:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4592:Dclk3	UTSW	9	111467895	missense	probably damaging	1.00
R4604:Dclk3	UTSW	9	111469185	missense	probably damaging	1.00
R4857:Dclk3	UTSW	9	111468648	missense	probably benign
R4932:Dclk3	UTSW	9	111468042	missense	possibly damaging	0.56
R5045:Dclk3	UTSW	9	111467788	missense	probably damaging	0.99
R5233:Dclk3	UTSW	9	111468681	missense	probably benign
R5338:Dclk3	UTSW	9	111469059	missense	possibly damaging	0.95
R5463:Dclk3	UTSW	9	111469260	missense	probably benign	0.26
R6822:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
R6995:Dclk3	UTSW	9	111467700	missense	possibly damaging	0.88
R7187:Dclk3	UTSW	9	111484996	missense	probably damaging	1.00
R7532:Dclk3	UTSW	9	111467528	missense	probably benign	0.03
R7534:Dclk3	UTSW	9	111468218	missense	probably benign
R7734:Dclk3	UTSW	9	111469095	missense	probably damaging	1.00
R8326:Dclk3	UTSW	9	111467534	missense	probably damaging	0.96
R8372:Dclk3	UTSW	9	111485013	missense	probably damaging	0.98
R8388:Dclk3	UTSW	9	111482745	missense	probably damaging	1.00
R8423:Dclk3	UTSW	9	111468719	missense	possibly damaging	0.93
R8493:Dclk3	UTSW	9	111468147	missense	probably benign	0.06
R9013:Dclk3	UTSW	9	111468498	missense	probably benign	0.31
R9114:Dclk3	UTSW	9	111488615	missense	probably benign	0.06
R9338:Dclk3	UTSW	9	111439305	missense	unknown
X0020:Dclk3	UTSW	9	111485075	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTATGGTCTCAGTGGTACAGG -3'
(R):5'- ACATACCCAGAGAGATGCATGC -3'

Sequencing Primer
(F):5'- GTCTCAGTGGTACAGGTTACACAC -3'
(R):5'- GAGTTTCAGCTCTCTGAGA -3'

Posted On

2022-05-16