Mutagenetix > Incidental Mutation

Incidental Mutation 'R9434:Or4a15'

713156

Institutional Source

Beutler Lab

Gene Symbol

Or4a15

Ensembl Gene

ENSMUSG00000075090

Gene Name

olfactory receptor family 4 subfamily A member 15

Synonyms

Olfr1234, MOR231-2, GA_x6K02T2Q125-50805620-50804676

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.106) question?

Stock #

R9434 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

89190357-89195209 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 89193692 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 27 (V27A)

Ref Sequence

ENSEMBL: ENSMUSP00000107168 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099783] [ENSMUST00000111543] [ENSMUST00000137692]

AlphaFold

A2AVK5

Predicted Effect

probably benign
Transcript: ENSMUST00000099783
AA Change: V27A

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000097371
Gene: ENSMUSG00000075090
AA Change: V27A

Domain	Start	End	E-Value	Type
Pfam:7tm_1	39	285	5.9e-32	PFAM
Pfam:7tm_4	137	278	1.5e-37	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111543
AA Change: V27A

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000107168
Gene: ENSMUSG00000075090
AA Change: V27A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	303	3.2e-46	PFAM
Pfam:7tm_1	39	285	7.2e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000137692
AA Change: V27A

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

98% (49/50)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam24	A	G	8: 41,133,284 (GRCm39)	I251V	probably benign	Het
Adgrv1	T	C	13: 81,666,292 (GRCm39)		probably benign	Het
Adipoq	A	G	16: 22,965,697 (GRCm39)		probably benign	Het
Adnp	C	T	2: 168,026,377 (GRCm39)	R306Q	probably damaging	Het
Akap11	A	T	14: 78,747,829 (GRCm39)	N1519K		Het
Bach1	C	G	16: 87,516,603 (GRCm39)	S381R	probably benign	Het
Cdc14a	T	C	3: 116,217,092 (GRCm39)	M15V	probably benign	Het
Cgn	T	C	3: 94,672,837 (GRCm39)	D947G	probably damaging	Het
Crhr2	A	T	6: 55,069,512 (GRCm39)	F381I	probably damaging	Het
Dmxl1	G	T	18: 50,010,788 (GRCm39)	A982S	probably damaging	Het
Efcab3	T	C	11: 104,899,863 (GRCm39)	V4375A	probably benign	Het
Epha5	T	C	5: 84,479,227 (GRCm39)	E259G	possibly damaging	Het
Erbb4	T	C	1: 68,081,773 (GRCm39)	D1087G	possibly damaging	Het
Fcgr2b	A	T	1: 170,793,385 (GRCm39)	S215T	probably benign	Het
Fsip2	A	T	2: 82,816,702 (GRCm39)	Y4145F	possibly damaging	Het
Fyb2	C	T	4: 104,847,534 (GRCm39)	T518M	probably damaging	Het
Galk1	C	T	11: 115,903,494 (GRCm39)	W4*	probably null	Het
Gmds	C	A	13: 32,284,369 (GRCm39)	D248Y	probably damaging	Het
Herc3	T	A	6: 58,853,846 (GRCm39)	F631I	probably benign	Het
Hhla1	T	C	15: 65,839,226 (GRCm39)	K55E	possibly damaging	Het
Il17rb	T	C	14: 29,728,054 (GRCm39)	E51G	probably damaging	Het
Ildr1	T	A	16: 36,529,862 (GRCm39)	L83Q	probably damaging	Het
Ints9	A	G	14: 65,245,506 (GRCm39)	I255V	probably benign	Het
Itga9	G	T	9: 118,636,315 (GRCm39)	D668Y	probably damaging	Het
Itpr3	G	A	17: 27,337,651 (GRCm39)		probably benign	Het
Klhl35	A	G	7: 99,119,547 (GRCm39)	Y344C	probably damaging	Het
Klra4	A	G	6: 130,040,083 (GRCm39)	V63A	possibly damaging	Het
Lgr4	A	G	2: 109,836,907 (GRCm39)	T414A	probably benign	Het
Lmbrd2	C	T	15: 9,157,314 (GRCm39)	T184M	probably benign	Het
Lrp1	T	C	10: 127,381,689 (GRCm39)	D3795G	possibly damaging	Het
N6amt1	T	A	16: 87,159,421 (GRCm39)	L109Q	possibly damaging	Het
Ncoa1	A	G	12: 4,365,755 (GRCm39)	C215R	probably benign	Het
Ngef	G	A	1: 87,408,315 (GRCm39)	S584F	possibly damaging	Het
Notch4	T	C	17: 34,801,673 (GRCm39)	C1174R	probably damaging	Het
Opa1	T	C	16: 29,404,874 (GRCm39)	I24T	probably benign	Het
Or10v1	A	C	19: 11,873,393 (GRCm39)	I3L	probably benign	Het
Or1e1b-ps1	A	T	11: 73,845,662 (GRCm39)	I49F	probably damaging	Het
Or1o4	T	C	17: 37,591,254 (GRCm39)	E19G	probably benign	Het
Pcnx2	A	G	8: 126,542,512 (GRCm39)	V1223A	probably benign	Het
Phf14	A	G	6: 11,933,492 (GRCm39)	K118R	unknown	Het
Plcd1	A	T	9: 118,905,231 (GRCm39)	M186K	probably damaging	Het
Selplg	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	5: 113,957,756 (GRCm39)		probably benign	Het
Shank1	G	A	7: 43,962,342 (GRCm39)	S71N	unknown	Het
Sumf1	A	T	6: 108,130,096 (GRCm39)	C233S	possibly damaging	Het
Tbc1d12	A	G	19: 38,902,461 (GRCm39)	K540R	probably benign	Het
Tmem150c	T	C	5: 100,240,643 (GRCm39)	N73S	probably damaging	Het
Tpra1	T	C	6: 88,888,774 (GRCm39)	S319P	probably benign	Het
Ttk	C	A	9: 83,750,143 (GRCm39)	Y699*	probably null	Het
Vmn1r196	T	A	13: 22,477,790 (GRCm39)	L143*	probably null	Het
Vmn2r84	A	G	10: 130,221,745 (GRCm39)	V825A	possibly damaging	Het
Zpld2	T	G	4: 133,929,553 (GRCm39)	T251P	probably benign	Het

Other mutations in Or4a15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02134:Or4a15	APN	2	89,193,172 (GRCm39)	missense	probably damaging	0.97
R0945:Or4a15	UTSW	2	89,193,599 (GRCm39)	missense	probably damaging	0.98
R1018:Or4a15	UTSW	2	89,193,523 (GRCm39)	missense	possibly damaging	0.95
R1419:Or4a15	UTSW	2	89,193,666 (GRCm39)	missense	probably damaging	1.00
R1872:Or4a15	UTSW	2	89,192,933 (GRCm39)	missense	probably damaging	1.00
R1929:Or4a15	UTSW	2	89,193,353 (GRCm39)	missense	probably benign	0.01
R2143:Or4a15	UTSW	2	89,193,447 (GRCm39)	missense	probably damaging	1.00
R3499:Or4a15	UTSW	2	89,193,294 (GRCm39)	missense	probably benign	0.07
R3776:Or4a15	UTSW	2	89,193,108 (GRCm39)	missense	possibly damaging	0.69
R4595:Or4a15	UTSW	2	89,193,669 (GRCm39)	missense	probably damaging	0.98
R6287:Or4a15	UTSW	2	89,193,363 (GRCm39)	nonsense	probably null
R7256:Or4a15	UTSW	2	89,192,838 (GRCm39)	missense	probably benign	0.21
R7516:Or4a15	UTSW	2	89,193,719 (GRCm39)	missense	probably benign	0.01
R7636:Or4a15	UTSW	2	89,193,583 (GRCm39)	nonsense	probably null
R7975:Or4a15	UTSW	2	89,193,413 (GRCm39)	missense	probably benign	0.23
R8240:Or4a15	UTSW	2	89,192,896 (GRCm39)	missense	probably benign	0.01
R8514:Or4a15	UTSW	2	89,193,573 (GRCm39)	missense	probably benign	0.06
R8680:Or4a15	UTSW	2	89,193,065 (GRCm39)	missense	probably benign	0.00
R8780:Or4a15	UTSW	2	89,193,652 (GRCm39)	missense	probably damaging	1.00
R8898:Or4a15	UTSW	2	89,192,957 (GRCm39)	missense	possibly damaging	0.56
R9402:Or4a15	UTSW	2	89,193,123 (GRCm39)	nonsense	probably null
R9451:Or4a15	UTSW	2	89,193,243 (GRCm39)	missense	probably damaging	1.00
R9632:Or4a15	UTSW	2	89,193,065 (GRCm39)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- TGAGTCATACAAGCCCGGAAG -3'
(R):5'- GGTGAGAAAATTACCACATTATAGCAC -3'

Sequencing Primer
(F):5'- ATGGTCTTTGTTTCATAGAGCAAGTC -3'
(R):5'- TCAGCACACTCAGAAGAG -3'

Posted On

2022-05-16