Mutagenetix > Incidental Mutation

Incidental Mutation 'R9435:Tas2r103'

713228

Institutional Source

Beutler Lab

Gene Symbol

Tas2r103

Ensembl Gene

ENSMUSG00000030196

Gene Name

taste receptor, type 2, member 103

Synonyms

mt2r63, T2R3, Tas2r10, EG667992, mGR03, TRB2, Tas2r3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R9435 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

133036163-133037101 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 133036723 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 127 (K127*)

Ref Sequence

ENSEMBL: ENSMUSP00000032317 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032317]

AlphaFold

Q9JKA3

Predicted Effect

probably null
Transcript: ENSMUST00000032317
AA Change: K127*

SMART Domains

Protein: ENSMUSP00000032317
Gene: ENSMUSG00000030196
AA Change: K127*

Domain	Start	End	E-Value	Type
Pfam:TAS2R	5	301	1.5e-79	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (58/58)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	A	T	14: 32,660,605	D1134E	probably benign	Het
Abca5	C	T	11: 110,292,085		probably null	Het
Acp2	A	G	2: 91,206,064	T169A	probably damaging	Het
Agtpbp1	A	T	13: 59,474,615	N932K	probably benign	Het
Aldh5a1	G	A	13: 24,937,310	R87C	probably damaging	Het
Bpifa1	G	T	2: 154,143,923	A53S	unknown	Het
Cacna2d2	T	C	9: 107,519,185	I667T	probably benign	Het
Catsperg1	T	A	7: 29,190,326	T784S	probably benign	Het
Ccdc93	C	T	1: 121,441,855	Q109*	probably null	Het
Cd46	C	G	1: 195,085,412	D114H	probably damaging	Het
Cd82	A	T	2: 93,437,395	L19Q	probably damaging	Het
Celsr1	T	C	15: 85,922,334		probably benign	Het
Cnksr1	A	G	4: 134,234,574	I180T	possibly damaging	Het
Drg2	T	C	11: 60,468,140	V362A	probably benign	Het
Eef2	T	A	10: 81,179,160	M231K	probably benign	Het
Egr2	T	C	10: 67,539,798	Y75H	probably damaging	Het
Exoc6	A	C	19: 37,597,097	Q473H	probably benign	Het
Focad	T	A	4: 88,348,839	M1029K	unknown	Het
Fpr-rs4	T	A	17: 18,022,129	S133T	probably benign	Het
Gbf1	T	C	19: 46,279,993	V1322A	probably benign	Het
Gm13212	G	C	4: 145,622,475	E161Q		Het
Gm572	T	C	4: 148,668,509	S282P	possibly damaging	Het
Gm7579	GGCTGTGGCTCCTGTGGGGGCTGCAAGGGAAGCTGTGGCTCCTGTGGGGGCTGCAAGGGAAGCTGTGGCTCCTGTGGGGGATGCAAGGGAGGCTGTGGCTCCTGTGGGGG	GGCTGTGGCTCCTGTGGGGGCTGCAAGGGAAGCTGTGGCTCCTGTGGGGGATGCAAGGGAGGCTGTGGCTCCTGTGGGGG	7: 142,212,045		probably benign	Het
Golph3l	T	C	3: 95,589,058	V31A	probably benign	Het
Iars2	A	T	1: 185,302,716	V610D	probably damaging	Het
Igsf6	T	A	7: 121,067,249	I203F	probably damaging	Het
Ildr2	A	G	1: 166,309,122	Y510C	probably damaging	Het
Iqcf4	C	A	9: 106,568,453	R165L	possibly damaging	Het
Itgb1bp1	T	G	12: 21,270,942	I183L	possibly damaging	Het
Itgb4	A	C	11: 116,005,029	S1414R	probably benign	Het
Kbtbd7	G	T	14: 79,427,504	A259S	probably benign	Het
Kif14	T	A	1: 136,473,436	D508E	possibly damaging	Het
Klc2	A	T	19: 5,109,634	D484E	possibly damaging	Het
Lamc2	T	C	1: 153,137,326	S630G	probably benign	Het
Myl6b	G	A	10: 128,495,197	T125M	possibly damaging	Het
Nbea	T	A	3: 56,035,888	K655N	possibly damaging	Het
Nkx3-2	T	C	5: 41,762,150	D165G	probably benign	Het
Npy	A	T	6: 49,827,501	R67S	probably damaging	Het
Nrg3	A	T	14: 39,472,599	C68S	possibly damaging	Het
Nup160	C	T	2: 90,729,794	P1288S	probably damaging	Het
Olfr672	T	C	7: 104,996,739	H55R	probably benign	Het
Olfr952	A	G	9: 39,426,210	L287P	probably benign	Het
Pam	T	A	1: 97,894,419	I287L	probably benign	Het
Phf10	T	C	17: 14,945,125	R490G	probably benign	Het
Prim2	T	C	1: 33,484,795	Y345C	probably damaging	Het
Ptchd3	C	T	11: 121,830,820	A173V	probably benign	Het
Rab8b	G	A	9: 66,848,630	R167W	probably damaging	Het
S100a3	A	G	3: 90,602,195	E49G	probably benign	Het
Slc41a1	T	A	1: 131,839,158	V138E	probably damaging	Het
Slit1	C	A	19: 41,603,325		probably null	Het
Sntg1	T	A	1: 8,363,590	H479L	probably damaging	Het
Stam	T	A	2: 14,115,990	C72S	probably damaging	Het
Sval2	G	T	6: 41,863,861	A93S	probably benign	Het
Trank1	C	T	9: 111,364,822	T638M	probably benign	Het
Utrn	T	A	10: 12,643,429	I2260F	probably damaging	Het
Zfp280d	T	C	9: 72,319,317		probably null	Het

Other mutations in Tas2r103

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Tas2r103	APN	6	133037056	missense	possibly damaging	0.86
IGL02332:Tas2r103	APN	6	133036512	missense	probably benign	0.21
IGL03122:Tas2r103	APN	6	133036909	missense	probably damaging	1.00
IGL03167:Tas2r103	APN	6	133036660	missense	probably damaging	0.98
R0380:Tas2r103	UTSW	6	133036203	missense	probably damaging	1.00
R0671:Tas2r103	UTSW	6	133036350	missense	probably benign	0.01
R1700:Tas2r103	UTSW	6	133036811	missense	probably damaging	1.00
R2098:Tas2r103	UTSW	6	133036597	missense	probably benign
R3977:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3979:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3980:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R4924:Tas2r103	UTSW	6	133036198	missense	probably benign	0.02
R5779:Tas2r103	UTSW	6	133036945	missense	probably benign	0.02
R5839:Tas2r103	UTSW	6	133036816	missense	probably benign	0.06
R6265:Tas2r103	UTSW	6	133036531	missense	probably damaging	1.00
R7768:Tas2r103	UTSW	6	133036849	missense	probably benign	0.06
R8851:Tas2r103	UTSW	6	133036933	missense

Predicted Primers

PCR Primer
(F):5'- TTGTCAAAGACACAGCGAATGG -3'
(R):5'- TTGTCACTAATCACAGGGTTCTTG -3'

Sequencing Primer
(F):5'- TGAGTGTAAACCCAGTGCTAATG -3'
(R):5'- TGGTATCATTATTGGACCCAGC -3'

Posted On

2022-05-16