Mutagenetix > Incidental Mutation

Incidental Mutation 'R9437:Or2w25'

713334

Institutional Source

Beutler Lab

Gene Symbol

Or2w25

Ensembl Gene

ENSMUSG00000044061

Gene Name

olfactory receptor family 2 subfamily W member 25

Synonyms

MOR256-51, GA_x6K02T0073K-490-1545, Olfr225

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R9437 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

59503792-59505051 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 59504195 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 135 (M135T)

Ref Sequence

ENSEMBL: ENSMUSP00000051482 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055276] [ENSMUST00000213169]

AlphaFold

E9PWF9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000055276
AA Change: M135T

PolyPhen 2 Score 0.920 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000051482
Gene: ENSMUSG00000044061
AA Change: M135T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	307	6.6e-47	PFAM
Pfam:7tm_1	40	289	4.3e-22	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213169
AA Change: M135T

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap8l	T	C	17: 32,553,608 (GRCm39)	S371G	probably benign	Het
Bdp1	A	G	13: 100,162,158 (GRCm39)	S2255P	probably benign	Het
Ccdc93	C	T	1: 121,369,584 (GRCm39)	Q109*	probably null	Het
Copa	G	A	1: 171,931,712 (GRCm39)	R321H	possibly damaging	Het
Cyp2b23	A	T	7: 26,372,199 (GRCm39)	D415E	possibly damaging	Het
Dcun1d2	T	C	8: 13,331,004 (GRCm39)	D42G	probably benign	Het
Ebf4	T	A	2: 130,202,005 (GRCm39)	S325T	probably benign	Het
Ergic2	T	C	6: 148,097,562 (GRCm39)	E137G	probably benign	Het
Etl4	T	C	2: 20,813,872 (GRCm39)	S1304P	probably benign	Het
Fam24b	A	T	7: 130,927,893 (GRCm39)	C99S	probably benign	Het
Fat4	A	G	3: 38,945,417 (GRCm39)	T1437A	probably benign	Het
Fgd5	A	G	6: 91,964,627 (GRCm39)	T287A	probably benign	Het
Fscb	G	T	12: 64,519,708 (GRCm39)	A586E	unknown	Het
Gm2244	A	T	14: 19,599,038 (GRCm39)	I86N	probably damaging	Het
Hnrnph3	T	A	10: 62,854,627 (GRCm39)	R55*	probably null	Het
Kcnk5	A	C	14: 20,192,468 (GRCm39)	I231S	probably damaging	Het
Krt10	A	G	11: 99,276,390 (GRCm39)	M87T	probably benign	Het
Ly6c2	C	G	15: 74,983,535 (GRCm39)		probably benign	Het
Map4	C	A	9: 109,864,155 (GRCm39)	T460K	possibly damaging	Het
Mok	A	G	12: 110,774,659 (GRCm39)	C388R	probably benign	Het
Ms4a3	T	C	19: 11,607,065 (GRCm39)	D200G	possibly damaging	Het
Ncapd2	A	G	6: 125,153,655 (GRCm39)	Y687H	probably damaging	Het
Nrp1	A	G	8: 129,187,108 (GRCm39)	T385A	probably benign	Het
Or56a41	A	T	7: 104,739,963 (GRCm39)	Y294*	probably null	Het
Osbp2	A	G	11: 3,664,581 (GRCm39)	S131P	probably damaging	Het
Pcdhga3	G	A	18: 37,808,144 (GRCm39)	R199H	probably damaging	Het
Peg10	T	TCCA	6: 4,756,451 (GRCm39)		probably benign	Het
Pigx	G	T	16: 31,918,310 (GRCm39)	D26E	probably benign	Het
Pkn3	T	C	2: 29,973,267 (GRCm39)	I363T	possibly damaging	Het
Psg16	T	A	7: 16,827,715 (GRCm39)	Y133N	probably damaging	Het
Pygl	A	G	12: 70,246,925 (GRCm39)	I407T	probably damaging	Het
Rc3h2	G	A	2: 37,272,841 (GRCm39)	A745V	possibly damaging	Het
Srgap1	T	C	10: 121,636,777 (GRCm39)	E703G	probably benign	Het
Susd4	T	C	1: 182,681,697 (GRCm39)		probably null	Het
Tbpl1	T	C	10: 22,587,838 (GRCm39)	N37S	probably damaging	Het
Tex264	T	C	9: 106,559,096 (GRCm39)	I77V	possibly damaging	Het

Other mutations in Or2w25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00090:Or2w25	APN	11	59,504,147 (GRCm39)	missense	possibly damaging	0.86
IGL01835:Or2w25	APN	11	59,504,165 (GRCm39)	missense	probably damaging	0.99
IGL02006:Or2w25	APN	11	59,503,985 (GRCm39)	missense	probably damaging	1.00
IGL03195:Or2w25	APN	11	59,504,629 (GRCm39)	missense	probably damaging	1.00
R0148:Or2w25	UTSW	11	59,504,320 (GRCm39)	missense	probably damaging	1.00
R0831:Or2w25	UTSW	11	59,504,480 (GRCm39)	missense	possibly damaging	0.70
R1924:Or2w25	UTSW	11	59,503,949 (GRCm39)	missense	possibly damaging	0.58
R2184:Or2w25	UTSW	11	59,503,964 (GRCm39)	missense	probably damaging	1.00
R5772:Or2w25	UTSW	11	59,504,712 (GRCm39)	missense	probably benign
R6986:Or2w25	UTSW	11	59,504,924 (GRCm39)	missense	possibly damaging	0.53
R7405:Or2w25	UTSW	11	59,504,899 (GRCm39)	missense	possibly damaging	0.53
R7755:Or2w25	UTSW	11	59,504,467 (GRCm39)	missense	probably damaging	0.97
R7851:Or2w25	UTSW	11	59,504,789 (GRCm39)	missense	probably benign	0.33
R9545:Or2w25	UTSW	11	59,504,275 (GRCm39)	missense	probably benign	0.27
R9562:Or2w25	UTSW	11	59,504,580 (GRCm39)	missense	probably damaging	1.00
R9565:Or2w25	UTSW	11	59,504,339 (GRCm39)	missense	probably damaging	1.00
Z1177:Or2w25	UTSW	11	59,504,398 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCTCTGTTACAGCACCAGTAG -3'
(R):5'- GCCAGGATGAAAATTAGGCCTTC -3'

Sequencing Primer
(F):5'- TCTGTTACAGCACCAGTAGCATCC -3'
(R):5'- GAAAATTAGGCCTTCTATCCAGC -3'

Posted On

2022-05-16