Mutagenetix > Incidental Mutation

Incidental Mutation 'R9441:Cyp2g1'

713644

Institutional Source

Beutler Lab

Gene Symbol

Cyp2g1

Ensembl Gene

ENSMUSG00000049685

Gene Name

cytochrome P450, family 2, subfamily g, polypeptide 1

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9441 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

26808892-26821205 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 26814635 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 222 (M222T)

Ref Sequence

ENSEMBL: ENSMUSP00000047150 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040944]

AlphaFold

Q9WV19

Predicted Effect

possibly damaging
Transcript: ENSMUST00000040944
AA Change: M222T

PolyPhen 2 Score 0.720 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000047150
Gene: ENSMUSG00000049685
AA Change: M222T

Domain	Start	End	E-Value	Type
low complexity region	12	23	N/A	INTRINSIC
Pfam:p450	34	491	4e-150	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for disruptions in this gene display an essentially normal phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930563M21Rik	C	A	9: 56,010,492	G20V	unknown	Het
Adam22	T	C	5: 8,111,974	T733A	possibly damaging	Het
Adam28	T	A	14: 68,637,494	N245Y	probably damaging	Het
Aldh9a1	C	G	1: 167,350,350	R39G	probably benign	Het
Armc3	A	C	2: 19,248,615	E189A	possibly damaging	Het
Atg9a	C	T	1: 75,186,442	C338Y	possibly damaging	Het
Card6	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	15: 5,098,691		probably benign	Het
Cars	G	A	7: 143,569,448	T560I	probably benign	Het
Casp8ap2	A	G	4: 32,645,873	K1649E	probably benign	Het
Ccdc88b	T	C	19: 6,855,845	E278G	probably damaging	Het
Cd84	G	A	1: 171,886,427		probably null	Het
Ces2g	G	T	8: 104,963,991	A135S	possibly damaging	Het
Cfh	T	C	1: 140,102,411	D926G	probably benign	Het
Chrm2	A	G	6: 36,524,020	T271A	probably benign	Het
Col6a3	G	T	1: 90,777,527	Y2824*	probably null	Het
Cth	T	A	3: 157,910,938	R196S	probably damaging	Het
Depdc5	T	A	5: 32,937,698	M773K	probably benign	Het
Dmbx1	G	A	4: 115,923,687	P39L	probably damaging	Het
Dst	G	C	1: 34,199,351	W1697C	probably damaging	Het
E330017A01Rik	G	A	16: 58,638,521		probably benign	Het
Eno1	C	T	4: 150,236,751		probably benign	Het
Erc2	G	T	14: 28,080,157	V761L	possibly damaging	Het
Frem1	A	T	4: 83,005,846	I292N	probably damaging	Het
Hip1	G	T	5: 135,431,717	L530M	possibly damaging	Het
Kcna2	A	G	3: 107,104,952	Q283R	probably benign	Het
Kndc1	G	A	7: 139,921,476	D894N	probably damaging	Het
Lats1	G	T	10: 7,702,917	G602W	probably damaging	Het
Lgals12	A	G	19: 7,603,991	L117P	probably damaging	Het
Mfsd4b1	A	T	10: 40,002,684	L406I	possibly damaging	Het
Msln	A	T	17: 25,750,757	M333K	probably benign	Het
Mup2	A	G	4: 60,139,740	V16A	probably benign	Het
Mybpc2	T	C	7: 44,516,906	E220G	probably null	Het
Myh6	A	G	14: 54,960,314	Y456H	probably benign	Het
Nlrp1a	A	G	11: 71,123,108	S439P	probably damaging	Het
Nyap1	T	C	5: 137,734,932	E613G	probably benign	Het
Olfr1358	G	A	10: 78,519,775	V56M	probably benign	Het
Parp8	C	T	13: 116,893,026	V554M	probably damaging	Het
Phpt1	A	T	2: 25,574,738	D34E	probably benign	Het
Ppip5k2	G	A	1: 97,745,196	S463L	probably benign	Het
Prss28	A	G	17: 25,311,241	Q173R	probably benign	Het
Rtel1	T	A	2: 181,347,067	W441R	possibly damaging	Het
Serbp1	T	C	6: 67,267,041		probably benign	Het
Skint5	T	C	4: 113,490,651	K1319E	unknown	Het
Slc35a4	G	A	18: 36,683,058	G314S	probably damaging	Het
Slc8a1	A	G	17: 81,649,069	I180T	probably damaging	Het
Spire1	G	A	18: 67,519,392	P205L	probably benign	Het
Tat	G	T	8: 109,993,915	G168W	probably damaging	Het
Thap1	CAGCATCTGCTCGGAGCA	CAGCA	8: 26,160,856		probably null	Het
Trh	C	T	6: 92,242,958	G126R	probably benign	Het
Ugcg	G	A	4: 59,207,843	G61R	probably damaging	Het
Uggt1	T	C	1: 36,221,225	T170A	probably benign	Het
Unc5b	G	A	10: 60,772,249	P702S	probably damaging	Het
Usp8	A	G	2: 126,720,153	D89G	possibly damaging	Het
Vmn2r1	T	A	3: 64,105,253	I845K	probably damaging	Het
Vnn3	A	G	10: 23,864,600	N267S	possibly damaging	Het
Xkr9	A	G	1: 13,701,363	R368G	possibly damaging	Het

Other mutations in Cyp2g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01134:Cyp2g1	APN	7	26809831	missense	probably benign	0.05
IGL01137:Cyp2g1	APN	7	26814259	missense	possibly damaging	0.87
IGL02052:Cyp2g1	APN	7	26814294	splice site	probably benign
IGL02338:Cyp2g1	APN	7	26814804	splice site	probably benign
IGL02452:Cyp2g1	APN	7	26811446	missense	probably benign	0.28
IGL02523:Cyp2g1	APN	7	26819187	missense	probably damaging	1.00
IGL03165:Cyp2g1	APN	7	26809776	missense	possibly damaging	0.94
IGL03230:Cyp2g1	APN	7	26819403	missense	probably damaging	1.00
PIT4472001:Cyp2g1	UTSW	7	26814194	missense	probably benign	0.28
R0106:Cyp2g1	UTSW	7	26814182	missense	probably damaging	1.00
R0106:Cyp2g1	UTSW	7	26814182	missense	probably damaging	1.00
R0380:Cyp2g1	UTSW	7	26814295	splice site	probably benign
R0697:Cyp2g1	UTSW	7	26814727	nonsense	probably null
R0830:Cyp2g1	UTSW	7	26814791	missense	probably benign	0.00
R1660:Cyp2g1	UTSW	7	26809682	critical splice acceptor site	probably null
R2093:Cyp2g1	UTSW	7	26819433	missense	probably benign	0.35
R2131:Cyp2g1	UTSW	7	26820710	missense	probably damaging	0.99
R4606:Cyp2g1	UTSW	7	26814154	missense	possibly damaging	0.80
R5030:Cyp2g1	UTSW	7	26820801	missense	probably benign	0.06
R5574:Cyp2g1	UTSW	7	26820740	missense	possibly damaging	0.81
R5877:Cyp2g1	UTSW	7	26816640	missense	possibly damaging	0.80
R6745:Cyp2g1	UTSW	7	26814179	missense	probably damaging	1.00
R7040:Cyp2g1	UTSW	7	26820759	missense	probably damaging	0.99
R7223:Cyp2g1	UTSW	7	26814632	missense	probably damaging	0.98
R7934:Cyp2g1	UTSW	7	26819193	missense	probably damaging	1.00
R8112:Cyp2g1	UTSW	7	26819461	missense	probably benign
R8177:Cyp2g1	UTSW	7	26819153	missense	probably damaging	1.00
R8194:Cyp2g1	UTSW	7	26814734	missense	possibly damaging	0.89
R9043:Cyp2g1	UTSW	7	26809831	missense	probably benign	0.05
R9406:Cyp2g1	UTSW	7	26819485	critical splice donor site	probably null
X0067:Cyp2g1	UTSW	7	26820762	missense	possibly damaging	0.70

Predicted Primers

PCR Primer
(F):5'- CCCTTGGAGGCCCTTAAATTTG -3'
(R):5'- ACCTGGTGCATCTTGATGAG -3'

Sequencing Primer
(F):5'- GGAGGCCCTTAAATTTGTCCAAG -3'
(R):5'- CCTGGTGCATCTTGATGAGAAAGC -3'

Posted On

2022-06-15