Mutagenetix > Incidental Mutation

Incidental Mutation 'R9445:Il16'

713877

Institutional Source

Beutler Lab

Gene Symbol

Il16

Ensembl Gene

ENSMUSG00000001741

Gene Name

interleukin 16

Synonyms

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.255) question?

Stock #

R9445 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

83292033-83394934 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 83337380 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 112 (K112*)

Ref Sequence

ENSEMBL: ENSMUSP00000001792 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001792] [ENSMUST00000153560]

AlphaFold

O54824

Predicted Effect

probably null
Transcript: ENSMUST00000001792
AA Change: K112*

SMART Domains

Protein: ENSMUSP00000001792
Gene: ENSMUSG00000001741
AA Change: K112*

Domain	Start	End	E-Value	Type
low complexity region	76	92	N/A	INTRINSIC
low complexity region	99	115	N/A	INTRINSIC
PDZ	222	300	6.5e-23	SMART
PDZ	361	438	3.89e-12	SMART
low complexity region	507	526	N/A	INTRINSIC
low complexity region	556	577	N/A	INTRINSIC
low complexity region	589	602	N/A	INTRINSIC
low complexity region	647	680	N/A	INTRINSIC
low complexity region	776	787	N/A	INTRINSIC
low complexity region	825	839	N/A	INTRINSIC
low complexity region	978	989	N/A	INTRINSIC
PDZ	1115	1192	3.6e-16	SMART
low complexity region	1201	1216	N/A	INTRINSIC
PDZ	1234	1310	4.11e-11	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000153560
AA Change: K112*

SMART Domains

Protein: ENSMUSP00000118516
Gene: ENSMUSG00000001741
AA Change: K112*

Domain	Start	End	E-Value	Type
low complexity region	76	92	N/A	INTRINSIC
low complexity region	99	115	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

99% (72/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
PHENOTYPE: Mice homozygous for a knock-out allele display a transient but consistent increase of thymidine incorporation in anti-CD3-stimulated CD4+ T cells, but fail to show a hyperproliferative T cell phenotype using BrdU labeling. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700024B05Rik	T	A	14: 41,818,174 (GRCm39)	H23Q	possibly damaging	Het
Abca14	T	C	7: 119,877,691 (GRCm39)	S1069P	probably benign	Het
Abo	A	G	2: 26,733,720 (GRCm39)	W160R	probably damaging	Het
Adgrb1	A	T	15: 74,435,807 (GRCm39)		probably benign	Het
Ahnak2	T	C	12: 112,745,978 (GRCm39)	Q1624R		Het
Amy2a1	T	A	3: 113,325,324 (GRCm39)	N90I	possibly damaging	Het
Bod1l	C	T	5: 41,974,619 (GRCm39)	V2232I	probably benign	Het
Brsk2	T	A	7: 141,538,149 (GRCm39)	H98Q	probably damaging	Het
Caskin2	C	T	11: 115,694,576 (GRCm39)	V342I	probably damaging	Het
Ccdc13	T	C	9: 121,627,156 (GRCm39)	N707S	probably benign	Het
Cdv3	T	C	9: 103,241,240 (GRCm39)	E109G	probably damaging	Het
Coro2a	C	T	4: 46,540,558 (GRCm39)	E454K	probably benign	Het
Cpq	A	T	15: 33,213,391 (GRCm39)	I137F	possibly damaging	Het
Ctsj	G	A	13: 61,151,838 (GRCm39)	T73I	possibly damaging	Het
Dchs2	A	G	3: 83,146,284 (GRCm39)	D710G	probably damaging	Het
Drd4	T	C	7: 140,872,162 (GRCm39)	V71A	probably damaging	Het
Eef2k	T	C	7: 120,457,694 (GRCm39)	C18R	probably benign	Het
Ercc4	C	A	16: 12,945,474 (GRCm39)	D386E	probably benign	Het
F2rl2	A	C	13: 95,837,622 (GRCm39)	L222F	probably benign	Het
Fkbp4	T	C	6: 128,413,580 (GRCm39)	D68G	probably damaging	Het
Fsip2	A	T	2: 82,806,132 (GRCm39)	D817V	probably damaging	Het
Gm19410	G	A	8: 36,239,652 (GRCm39)	R116H	possibly damaging	Het
Gm9376	A	T	14: 118,504,502 (GRCm39)		probably benign	Het
Golim4	A	T	3: 75,813,775 (GRCm39)	F150I	probably damaging	Het
Gtpbp2	G	A	17: 46,478,757 (GRCm39)	V525M	probably damaging	Het
Hook1	A	G	4: 95,901,499 (GRCm39)	N486D	probably benign	Het
Hook1	A	G	4: 95,903,049 (GRCm39)	S513G	probably benign	Het
Hoxd9	A	T	2: 74,528,415 (GRCm39)	T6S	probably damaging	Het
Hsd3b2	A	T	3: 98,619,051 (GRCm39)	L298Q	possibly damaging	Het
Idh3b	A	C	2: 130,123,572 (GRCm39)	S172A	probably benign	Het
Ifnar1	C	T	16: 91,292,367 (GRCm39)	P207L	probably benign	Het
Ighv5-17	T	A	12: 113,822,858 (GRCm39)	T88S	possibly damaging	Het
Irag1	A	T	7: 110,545,161 (GRCm39)	I45N	possibly damaging	Het
Kalrn	T	A	16: 33,805,600 (GRCm39)	I2646F	probably benign	Het
Kcnt1	G	T	2: 25,767,959 (GRCm39)	G23C	probably damaging	Het
Kirrel2	T	A	7: 30,150,260 (GRCm39)	I523F	probably damaging	Het
Lrrc3b	A	T	14: 15,358,552 (GRCm38)	L18H	probably damaging	Het
Med13l	T	A	5: 118,862,214 (GRCm39)	S386T	probably benign	Het
Mettl6	C	T	14: 31,209,527 (GRCm39)		probably null	Het
Mocs2	C	T	13: 114,961,879 (GRCm39)	A120V	possibly damaging	Het
Mrpl39	T	C	16: 84,531,346 (GRCm39)	T74A	probably benign	Het
Myh2	T	C	11: 67,069,754 (GRCm39)	F367L	probably damaging	Het
N4bp1	G	A	8: 87,587,238 (GRCm39)	Q567*	probably null	Het
Naf1	A	G	8: 67,336,097 (GRCm39)	I341M	probably damaging	Het
Ncoa6	A	T	2: 155,250,063 (GRCm39)	N1080K	probably benign	Het
Nell1	C	A	7: 49,632,474 (GRCm39)	L36I	possibly damaging	Het
Nrxn2	A	G	19: 6,522,448 (GRCm39)	D446G	probably damaging	Het
Nrxn3	T	A	12: 89,499,737 (GRCm39)	C709*	probably null	Het
Or13p4	T	G	4: 118,547,416 (GRCm39)	T78P	probably damaging	Het
Or2ag17	A	G	7: 106,389,464 (GRCm39)	V248A	probably damaging	Het
Or2n1	A	C	17: 38,486,694 (GRCm39)	T240P	probably damaging	Het
Or2t26	C	G	11: 49,039,879 (GRCm39)	A265G	probably benign	Het
Or8g19	T	A	9: 39,055,766 (GRCm39)	Y123*	probably null	Het
Ovgp1	T	C	3: 105,893,883 (GRCm39)		probably benign	Het
Pbx3	A	G	2: 34,114,555 (GRCm39)		probably benign	Het
Pcnx1	T	C	12: 81,964,981 (GRCm39)	S383P	probably damaging	Het
Pde6h	T	G	6: 136,936,359 (GRCm39)	F34C	probably damaging	Het
Pnliprp1	A	G	19: 58,720,628 (GRCm39)		probably benign	Het
Pramel21	T	C	4: 143,343,795 (GRCm39)	L365P	probably damaging	Het
Prl8a9	C	T	13: 27,748,498 (GRCm39)		probably null	Het
Qdpr	T	C	5: 45,596,669 (GRCm39)	N165S	probably benign	Het
Rabl2	A	G	15: 89,468,148 (GRCm39)	F158L	probably damaging	Het
Rrp7a	G	T	15: 83,004,084 (GRCm39)	C117*	probably null	Het
Ryr2	T	C	13: 11,787,463 (GRCm39)	Y970C	probably damaging	Het
Sf3b3	T	C	8: 111,552,774 (GRCm39)	T503A	possibly damaging	Het
Sipa1	T	C	19: 5,704,198 (GRCm39)	E708G	probably damaging	Het
Skor2	T	A	18: 76,948,811 (GRCm39)	S844R	possibly damaging	Het
Slc6a7	C	T	18: 61,138,815 (GRCm39)	C231Y	probably damaging	Het
Smgc	A	T	15: 91,729,665 (GRCm39)	E206D	probably benign	Het
Tanc2	T	C	11: 105,758,290 (GRCm39)	S684P	possibly damaging	Het
Tep1	T	C	14: 51,082,967 (GRCm39)	T1014A	possibly damaging	Het
Tex19.1	A	G	11: 121,038,283 (GRCm39)	S214G	probably benign	Het
Trmt6	G	A	2: 132,650,774 (GRCm39)	S278L	probably benign	Het
Use1	T	C	8: 71,821,200 (GRCm39)	S156P	probably benign	Het
Znrf1	G	A	8: 112,335,954 (GRCm39)	V152M	probably damaging	Het

Other mutations in Il16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Il16	APN	7	83,301,666 (GRCm39)	missense	probably benign	0.02
IGL01743:Il16	APN	7	83,301,507 (GRCm39)	missense	probably benign	0.00
IGL01770:Il16	APN	7	83,322,234 (GRCm39)	splice site	probably benign
IGL02025:Il16	APN	7	83,302,056 (GRCm39)	missense	probably damaging	1.00
IGL02317:Il16	APN	7	83,316,097 (GRCm39)	missense	probably damaging	1.00
IGL02412:Il16	APN	7	83,301,899 (GRCm39)	missense	probably benign	0.03
IGL02550:Il16	APN	7	83,323,704 (GRCm39)	missense	possibly damaging	0.90
IGL02568:Il16	APN	7	83,310,484 (GRCm39)	missense	probably damaging	1.00
IGL02578:Il16	APN	7	83,327,194 (GRCm39)	critical splice donor site	probably null
IGL02815:Il16	APN	7	83,300,249 (GRCm39)	missense	probably damaging	0.98
IGL03157:Il16	APN	7	83,371,611 (GRCm39)	missense	probably damaging	1.00
IGL03161:Il16	APN	7	83,371,707 (GRCm39)	missense	probably damaging	1.00
IGL03188:Il16	APN	7	83,337,371 (GRCm39)	missense	probably benign	0.00
IGL03213:Il16	APN	7	83,295,708 (GRCm39)	missense	probably damaging	1.00
IGL03274:Il16	APN	7	83,310,442 (GRCm39)	missense	probably damaging	1.00
R0201:Il16	UTSW	7	83,371,516 (GRCm39)	missense	probably damaging	0.99
R0309:Il16	UTSW	7	83,371,762 (GRCm39)	missense	probably damaging	1.00
R0597:Il16	UTSW	7	83,327,183 (GRCm39)	splice site	probably benign
R0942:Il16	UTSW	7	83,312,349 (GRCm39)	missense	probably benign	0.01
R1018:Il16	UTSW	7	83,323,746 (GRCm39)	missense	probably damaging	1.00
R1434:Il16	UTSW	7	83,304,520 (GRCm39)	missense	probably benign
R1715:Il16	UTSW	7	83,297,936 (GRCm39)	missense	probably benign	0.01
R2179:Il16	UTSW	7	83,337,287 (GRCm39)	splice site	probably null
R2520:Il16	UTSW	7	83,301,202 (GRCm39)	missense	probably benign	0.03
R3425:Il16	UTSW	7	83,293,248 (GRCm39)	missense	probably damaging	1.00
R3761:Il16	UTSW	7	83,300,093 (GRCm39)	missense	possibly damaging	0.96
R3943:Il16	UTSW	7	83,301,223 (GRCm39)	missense	probably damaging	0.97
R4470:Il16	UTSW	7	83,300,046 (GRCm39)	intron	probably benign
R4530:Il16	UTSW	7	83,330,518 (GRCm39)	intron	probably benign
R4583:Il16	UTSW	7	83,332,107 (GRCm39)	missense	probably damaging	1.00
R4777:Il16	UTSW	7	83,300,104 (GRCm39)	missense	probably benign	0.14
R4874:Il16	UTSW	7	83,310,153 (GRCm39)	missense	possibly damaging	0.56
R4876:Il16	UTSW	7	83,322,302 (GRCm39)	missense	probably benign
R5677:Il16	UTSW	7	83,323,761 (GRCm39)	missense	probably damaging	1.00
R5686:Il16	UTSW	7	83,297,936 (GRCm39)	missense	probably benign	0.36
R5920:Il16	UTSW	7	83,301,552 (GRCm39)	missense	probably benign	0.03
R6115:Il16	UTSW	7	83,301,775 (GRCm39)	nonsense	probably null
R6459:Il16	UTSW	7	83,371,536 (GRCm39)	missense	probably damaging	1.00
R6459:Il16	UTSW	7	83,371,529 (GRCm39)	missense	probably damaging	1.00
R6601:Il16	UTSW	7	83,371,677 (GRCm39)	missense	probably damaging	1.00
R6616:Il16	UTSW	7	83,295,684 (GRCm39)	missense	probably benign	0.37
R6642:Il16	UTSW	7	83,337,335 (GRCm39)	missense	probably benign	0.03
R6721:Il16	UTSW	7	83,312,270 (GRCm39)	critical splice donor site	probably null
R7009:Il16	UTSW	7	83,295,596 (GRCm39)	missense	probably benign
R7144:Il16	UTSW	7	83,295,659 (GRCm39)	missense	probably damaging	0.97
R7346:Il16	UTSW	7	83,293,249 (GRCm39)	missense	probably damaging	1.00
R7403:Il16	UTSW	7	83,319,343 (GRCm39)	missense	probably damaging	1.00
R7499:Il16	UTSW	7	83,323,702 (GRCm39)	missense	probably damaging	0.99
R7814:Il16	UTSW	7	83,319,348 (GRCm39)	missense	possibly damaging	0.46
R7941:Il16	UTSW	7	83,332,037 (GRCm39)	missense	probably damaging	0.98
R8098:Il16	UTSW	7	83,295,767 (GRCm39)	missense	probably damaging	1.00
R8317:Il16	UTSW	7	83,304,538 (GRCm39)	missense	probably benign
R8437:Il16	UTSW	7	83,301,351 (GRCm39)	missense	probably damaging	1.00
R9094:Il16	UTSW	7	83,301,559 (GRCm39)	missense	probably benign
R9267:Il16	UTSW	7	83,371,757 (GRCm39)	missense	probably benign	0.01
R9595:Il16	UTSW	7	83,322,273 (GRCm39)	nonsense	probably null
R9651:Il16	UTSW	7	83,332,064 (GRCm39)	missense	probably damaging	0.96
Z1176:Il16	UTSW	7	83,302,035 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- TGCTAGTTCTGATATTCAGGAGC -3'
(R):5'- AAATCCAGGTCTCAGAGTGCC -3'

Sequencing Primer
(F):5'- AATCCATAGGTCTCCAGGTGATG -3'
(R):5'- AGGTCTCAGAGTGCCTGCTAC -3'

Posted On

2022-06-15