Mutagenetix > Incidental Mutation

Incidental Mutation 'R9446:Ralgapa1'

713953

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, Tulip1, 2310003F20Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.802) question?

Stock #

R9446 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

55649681-55867952 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 55754808 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 1161 (V1161A)

Ref Sequence

ENSEMBL: ENSMUSP00000082503 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

probably damaging
Transcript: ENSMUST00000085385
AA Change: V1161A

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027
AA Change: V1161A

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000110687
AA Change: V1161A

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027
AA Change: V1161A

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000219432
AA Change: V1208A

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000220367
AA Change: V1161A

PolyPhen 2 Score 0.897 (Sensitivity: 0.82; Specificity: 0.94)

Predicted Effect

probably benign
Transcript: ENSMUST00000226244
AA Change: V1617A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adal	T	G	2: 120,976,530 (GRCm39)	I90S	probably null	Het
Adck2	G	T	6: 39,551,221 (GRCm39)		probably benign	Het
Adgrf3	G	T	5: 30,401,957 (GRCm39)	D690E	probably benign	Het
Aff3	C	T	1: 38,574,337 (GRCm39)	G175S	probably benign	Het
Atf7	T	C	15: 102,459,923 (GRCm39)	H159R	probably damaging	Het
BB014433	GGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCAGGTTGGGGGCTCTGCACACAGCTTTGGAGGTGTACACACCGGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGGGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCAGGTTGGGGGCTCTGCACACAGCTTTGGAGGTGTACACACCGGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTACACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGG	GGGTTGGGGGCTCTGCACACAGCTTTGGAGGTGTACACACCGGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGGGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCAGGTTGGGGGCTCTGCACACAGCTTTGGAGGTGTACACACCGGGGTTGGGGCCTCTGCACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTACACACAGCTTTGGAGGTGTACACACCCGGGTTGGGGCCTCTGCACACAGCTTTGG	8: 15,091,810 (GRCm39)		probably benign	Het
Birc6	A	G	17: 74,925,491 (GRCm39)	K2241R	probably damaging	Het
Cacna1s	A	G	1: 136,045,362 (GRCm39)	E1670G	probably benign	Het
Clca4b	C	T	3: 144,638,134 (GRCm39)	A43T	probably benign	Het
Clock	T	A	5: 76,396,288 (GRCm39)	I154L	probably benign	Het
Dnah10	A	T	5: 124,823,677 (GRCm39)	D566V	probably damaging	Het
Eml1	T	C	12: 108,481,465 (GRCm39)	W443R	probably damaging	Het
Ghitm	A	G	14: 36,853,606 (GRCm39)	I78T	possibly damaging	Het
Gm4181	A	T	14: 51,873,062 (GRCm39)	M1K	probably null	Het
Gm9195	T	C	14: 72,717,957 (GRCm39)	Y187C	probably damaging	Het
Htt	A	G	5: 34,919,272 (GRCm39)	M8V	probably benign	Het
Ighv1-9	T	C	12: 114,547,388 (GRCm39)	Y51C	probably benign	Het
Kif13b	G	A	14: 64,984,470 (GRCm39)	E636K	probably damaging	Het
Mamdc4	T	A	2: 25,453,645 (GRCm39)	M1179L	probably benign	Het
Map3k4	G	A	17: 12,451,375 (GRCm39)	A1506V	probably damaging	Het
Mbtd1	T	C	11: 93,834,508 (GRCm39)	S622P	unknown	Het
Med13l	T	A	5: 118,876,567 (GRCm39)	D914E	probably benign	Het
Mettl9	T	A	7: 120,675,531 (GRCm39)	D310E	possibly damaging	Het
Myh13	T	G	11: 67,255,325 (GRCm39)	V1565G	probably benign	Het
Nrf1	A	G	6: 30,090,019 (GRCm39)	T33A	probably damaging	Het
Oas1d	A	G	5: 121,054,947 (GRCm39)	K173R	probably benign	Het
Or12e10	A	T	2: 87,640,199 (GRCm39)	M12L	probably benign	Het
Or1e34	T	A	11: 73,778,530 (GRCm39)	T223S	probably benign	Het
Or4k6	A	G	14: 50,475,974 (GRCm39)	Y123H	probably damaging	Het
Or8g54	A	T	9: 39,707,342 (GRCm39)	S224C	probably damaging	Het
Pde6a	T	A	18: 61,419,067 (GRCm39)	D814E	probably benign	Het
Pilrb2	T	C	5: 137,869,429 (GRCm39)	D57G	possibly damaging	Het
Psg21	T	A	7: 18,388,865 (GRCm39)	T76S	probably benign	Het
Rtl1	C	G	12: 109,556,604 (GRCm39)	*1745S	probably null	Het
Samd8	T	C	14: 21,833,769 (GRCm39)	C296R		Het
Selplg	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	5: 113,957,756 (GRCm39)		probably benign	Het
Sfmbt1	T	C	14: 30,506,697 (GRCm39)	I247T	possibly damaging	Het
Smarca4	G	A	9: 21,547,155 (GRCm39)	A227T	unknown	Het
Trmt6	G	A	2: 132,650,774 (GRCm39)	S278L	probably benign	Het
Trpm6	T	C	19: 18,815,462 (GRCm39)	S1183P	possibly damaging	Het
Trpm7	T	A	2: 126,672,185 (GRCm39)		probably null	Het
Wnt10a	C	A	1: 74,842,728 (GRCm39)	F401L	possibly damaging	Het
Zfp970	A	T	2: 177,167,064 (GRCm39)	K213*	probably null	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,769,558 (GRCm39)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,793,970 (GRCm39)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,749,237 (GRCm39)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,756,360 (GRCm39)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,689,144 (GRCm39)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,689,133 (GRCm39)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,824,101 (GRCm39)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,766,442 (GRCm39)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,689,262 (GRCm39)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,754,862 (GRCm39)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,689,234 (GRCm39)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,759,450 (GRCm39)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,720,292 (GRCm39)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,723,202 (GRCm39)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,763,854 (GRCm39)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,804,736 (GRCm39)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
bantam	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
wickedwarlock	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,842,438 (GRCm39)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,741,942 (GRCm39)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,833,048 (GRCm39)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,786,290 (GRCm39)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
R0361:Ralgapa1	UTSW	12	55,723,354 (GRCm39)	missense	possibly damaging	0.93
R0385:Ralgapa1	UTSW	12	55,723,823 (GRCm39)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,754,852 (GRCm39)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,736,576 (GRCm39)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,723,550 (GRCm39)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,829,670 (GRCm39)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,842,483 (GRCm39)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,712,448 (GRCm39)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,723,366 (GRCm39)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0863:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,809,446 (GRCm39)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,754,763 (GRCm39)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,723,711 (GRCm39)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,788,265 (GRCm39)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,731,309 (GRCm39)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,817,488 (GRCm39)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,788,321 (GRCm39)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,809,388 (GRCm39)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,723,552 (GRCm39)	missense	possibly damaging	0.79
R1714:Ralgapa1	UTSW	12	55,689,174 (GRCm39)	missense	probably damaging	1.00
R1832:Ralgapa1	UTSW	12	55,804,752 (GRCm39)	missense	probably benign	0.03
R1870:Ralgapa1	UTSW	12	55,723,817 (GRCm39)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,833,107 (GRCm39)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,723,811 (GRCm39)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,741,945 (GRCm39)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,822,973 (GRCm39)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,723,909 (GRCm39)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,867,540 (GRCm39)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,705,922 (GRCm39)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,817,398 (GRCm39)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,741,928 (GRCm39)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,705,915 (GRCm39)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,745,552 (GRCm39)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,842,486 (GRCm39)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,786,115 (GRCm39)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,724,061 (GRCm39)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,740,243 (GRCm39)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,759,533 (GRCm39)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,841,778 (GRCm39)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,723,222 (GRCm39)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,745,588 (GRCm39)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,809,359 (GRCm39)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,764,899 (GRCm39)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,659,508 (GRCm39)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,712,459 (GRCm39)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,822,937 (GRCm39)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,804,817 (GRCm39)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,723,582 (GRCm39)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,766,408 (GRCm39)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,723,495 (GRCm39)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,659,523 (GRCm39)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,867,551 (GRCm39)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,723,898 (GRCm39)	missense	probably damaging	1.00
R5950:Ralgapa1	UTSW	12	55,785,050 (GRCm39)	missense	possibly damaging	0.58
R5980:Ralgapa1	UTSW	12	55,817,401 (GRCm39)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,730,827 (GRCm39)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,804,709 (GRCm39)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,793,931 (GRCm39)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,730,695 (GRCm39)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,766,446 (GRCm39)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,785,104 (GRCm39)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,809,512 (GRCm39)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,651,058 (GRCm39)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,832,997 (GRCm39)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,822,976 (GRCm39)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,804,844 (GRCm39)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,867,508 (GRCm39)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,768,361 (GRCm39)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,650,976 (GRCm39)	missense	probably damaging	1.00
R7254:Ralgapa1	UTSW	12	55,741,978 (GRCm39)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,755,789 (GRCm39)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,759,457 (GRCm39)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,765,013 (GRCm39)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,705,928 (GRCm39)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,756,341 (GRCm39)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,756,340 (GRCm39)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,788,298 (GRCm39)	missense	probably benign
R7763:Ralgapa1	UTSW	12	55,804,740 (GRCm39)	missense	probably benign	0.28
R7791:Ralgapa1	UTSW	12	55,788,304 (GRCm39)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,766,413 (GRCm39)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,659,423 (GRCm39)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,793,934 (GRCm39)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,744,663 (GRCm39)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,749,242 (GRCm39)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,829,631 (GRCm39)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,769,699 (GRCm39)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,788,308 (GRCm39)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,705,847 (GRCm39)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,723,303 (GRCm39)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,786,198 (GRCm39)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,785,002 (GRCm39)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,785,101 (GRCm39)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,749,345 (GRCm39)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,867,546 (GRCm39)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,723,791 (GRCm39)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,652,314 (GRCm39)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,723,351 (GRCm39)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,781,881 (GRCm39)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,755,843 (GRCm39)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9684:Ralgapa1	UTSW	12	55,659,485 (GRCm39)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,755,865 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTCATAAAGTGAAAGTGAACGATAT -3'
(R):5'- TTCATTAGTGAAATTGGACCAACATA -3'

Sequencing Primer
(F):5'- CAGGCTATAGTCTATCACTGCAGG -3'
(R):5'- TGAAAACAGCTATTGCTTACT -3'

Posted On

2022-06-15