Incidental Mutation 'R9447:Fstl4'
ID 714009
Institutional Source Beutler Lab
Gene Symbol Fstl4
Ensembl Gene ENSMUSG00000036264
Gene Name follistatin-like 4
Synonyms SPIG1, B230374F23Rik
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.151) question?
Stock # R9447 (G1)
Quality Score 225.009
Status Not validated
Chromosome 11
Chromosomal Location 52655461-53079365 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 53077166 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Cysteine to Phenylalanine at position 641 (C641F)
Ref Sequence ENSEMBL: ENSMUSP00000042007 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000036796]
AlphaFold Q5STE3
Predicted Effect probably damaging
Transcript: ENSMUST00000036796
AA Change: C641F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000042007
Gene: ENSMUSG00000036264
AA Change: C641F

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
KAZAL 87 132 4.7e-13 SMART
Blast:IG_like 215 241 6e-7 BLAST
IGc2 260 327 1.9e-6 SMART
IGc2 352 419 1e-14 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.3%
Validation Efficiency
MGI Phenotype PHENOTYPE: Homozygous null mice were born at expected Mendelian ratio and healthy, fertile, apparently normal with normal retinal laminar structure. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 64 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aadacl2fm2 T A 3: 59,651,051 (GRCm39) Y58N probably damaging Het
Ago4 G A 4: 126,402,151 (GRCm39) P572S probably benign Het
Akr1c19 A G 13: 4,296,838 (GRCm39) I295V probably benign Het
Cadps2 A T 6: 23,323,297 (GRCm39) I1007K probably damaging Het
Cd47 C A 16: 49,715,822 (GRCm39) T196K Het
Cdrt4 T G 11: 62,883,418 (GRCm39) L40R probably damaging Het
Cox16 A T 12: 81,406,109 (GRCm39) C72S probably benign Het
Cyp2t4 G A 7: 26,854,717 (GRCm39) V66M possibly damaging Het
Daxx A T 17: 34,132,247 (GRCm39) D497V unknown Het
Ddt A G 10: 75,608,671 (GRCm39) V62A possibly damaging Het
Dhx57 A G 17: 80,549,523 (GRCm39) V1294A probably damaging Het
Dnaaf5 A G 5: 139,163,743 (GRCm39) T667A probably damaging Het
Dnase2a A C 8: 85,635,786 (GRCm39) S90R probably damaging Het
Dytn A G 1: 63,700,302 (GRCm39) V276A Het
Efcab8 G C 2: 153,646,861 (GRCm39) V397L unknown Het
Enpp6 A T 8: 47,483,600 (GRCm39) R131W probably damaging Het
Epm2a C T 10: 11,324,432 (GRCm39) H174Y possibly damaging Het
Fcho1 A G 8: 72,169,913 (GRCm39) L70P probably damaging Het
Fhip1b G T 7: 105,034,155 (GRCm39) T492K probably benign Het
Ganab C T 19: 8,886,894 (GRCm39) H327Y probably damaging Het
Gkn1 A G 6: 87,323,322 (GRCm39) Y164H probably benign Het
Gm57858 A G 3: 36,074,195 (GRCm39) V318A possibly damaging Het
Hpf1 A G 8: 61,348,618 (GRCm39) D111G probably damaging Het
Hs3st2 C T 7: 120,992,289 (GRCm39) R113W probably damaging Het
Ighm A T 12: 113,384,794 (GRCm39) L353Q Het
Krbox5 A G 13: 67,981,953 (GRCm39) E55G probably damaging Het
Ksr1 T C 11: 78,909,159 (GRCm39) D782G unknown Het
Lpin2 A G 17: 71,539,087 (GRCm39) K392E unknown Het
Lrrc4 A G 6: 28,830,650 (GRCm39) S322P probably benign Het
Man2a1 T C 17: 64,966,001 (GRCm39) V313A possibly damaging Het
Mctp1 A G 13: 76,727,904 (GRCm39) S9G probably benign Het
Morn5 A T 2: 35,969,525 (GRCm39) probably null Het
Mroh2b C T 15: 4,960,823 (GRCm39) A795V probably damaging Het
Ncf4 G T 15: 78,146,499 (GRCm39) A310S probably benign Het
Nedd4 T C 9: 72,577,381 (GRCm39) Y69H probably benign Het
Nras T C 3: 102,967,673 (GRCm39) F90L possibly damaging Het
Nrxn3 T C 12: 89,221,678 (GRCm39) F113L probably benign Het
Ntsr1 C T 2: 180,180,540 (GRCm39) T282I probably benign Het
Or1e1d-ps1 G T 11: 73,819,617 (GRCm39) C189F probably benign Het
Osbpl3 T A 6: 50,321,857 (GRCm39) K310* probably null Het
Pcca T C 14: 122,854,290 (GRCm39) V194A probably damaging Het
Plekha5 A G 6: 140,525,192 (GRCm39) Y900C probably damaging Het
Prss22 T C 17: 24,212,837 (GRCm39) D300G probably benign Het
Rad17 A T 13: 100,764,119 (GRCm39) Y451N probably damaging Het
Rnf181 G T 6: 72,337,570 (GRCm39) T97K probably damaging Het
Sec24d T C 3: 123,084,162 (GRCm39) S114P probably benign Het
Siae C A 9: 37,557,743 (GRCm39) Q517K probably benign Het
Smurf2 A T 11: 106,715,548 (GRCm39) V653D probably damaging Het
Sqor C A 2: 122,649,520 (GRCm39) T359K possibly damaging Het
Taf6 A T 5: 138,176,970 (GRCm39) *637R probably null Het
Tas2r109 A C 6: 132,957,270 (GRCm39) M220R probably damaging Het
Tlr2 T A 3: 83,748,445 (GRCm39) probably null Het
Tm7sf3 C T 6: 146,525,179 (GRCm39) D89N possibly damaging Het
Tmem8b T G 4: 43,685,766 (GRCm39) L179R probably damaging Het
Tnfrsf10b T A 14: 70,013,608 (GRCm39) H179Q probably damaging Het
Trip11 T A 12: 101,850,148 (GRCm39) K1305N probably damaging Het
Ttn C A 2: 76,582,862 (GRCm39) W22677L probably damaging Het
Ube2r2 T C 4: 41,190,655 (GRCm39) V183A probably damaging Het
Ubqln4 T A 3: 88,464,124 (GRCm39) D208E probably benign Het
Ugt2a3 T A 5: 87,473,330 (GRCm39) N529I probably benign Het
Vmn1r125 A G 7: 21,006,627 (GRCm39) N175S probably benign Het
Xirp2 T A 2: 67,338,950 (GRCm39) I397K probably damaging Het
Zfp821 G A 8: 110,450,816 (GRCm39) V270I probably damaging Het
Zfp935 A G 13: 62,602,842 (GRCm39) V99A possibly damaging Het
Other mutations in Fstl4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00093:Fstl4 APN 11 53,077,102 (GRCm39) missense probably benign 0.01
IGL00885:Fstl4 APN 11 53,039,809 (GRCm39) missense possibly damaging 0.90
IGL00915:Fstl4 APN 11 53,067,825 (GRCm39) missense probably benign
IGL00933:Fstl4 APN 11 53,077,588 (GRCm39) missense possibly damaging 0.80
IGL01022:Fstl4 APN 11 53,077,568 (GRCm39) missense probably benign 0.18
IGL01121:Fstl4 APN 11 52,705,464 (GRCm39) missense probably benign 0.00
IGL01656:Fstl4 APN 11 52,891,201 (GRCm39) missense probably damaging 1.00
IGL01805:Fstl4 APN 11 53,077,184 (GRCm39) missense probably damaging 1.00
IGL01997:Fstl4 APN 11 53,053,881 (GRCm39) nonsense probably null
IGL02386:Fstl4 APN 11 52,664,698 (GRCm39) missense probably benign 0.21
IGL02536:Fstl4 APN 11 53,024,851 (GRCm39) splice site probably benign
IGL02807:Fstl4 APN 11 53,077,501 (GRCm39) missense probably benign 0.03
IGL03037:Fstl4 APN 11 53,059,050 (GRCm39) missense possibly damaging 0.83
R0462:Fstl4 UTSW 11 53,077,229 (GRCm39) missense probably benign 0.09
R1190:Fstl4 UTSW 11 52,959,373 (GRCm39) missense probably benign
R1300:Fstl4 UTSW 11 52,959,454 (GRCm39) missense probably benign
R1626:Fstl4 UTSW 11 52,891,117 (GRCm39) nonsense probably null
R1695:Fstl4 UTSW 11 53,056,705 (GRCm39) splice site probably null
R1699:Fstl4 UTSW 11 53,059,005 (GRCm39) missense possibly damaging 0.81
R1727:Fstl4 UTSW 11 52,959,478 (GRCm39) missense probably damaging 1.00
R1752:Fstl4 UTSW 11 53,077,622 (GRCm39) missense probably benign 0.09
R1866:Fstl4 UTSW 11 53,077,225 (GRCm39) missense probably benign 0.00
R4689:Fstl4 UTSW 11 52,959,477 (GRCm39) nonsense probably null
R5126:Fstl4 UTSW 11 53,077,388 (GRCm39) missense possibly damaging 0.71
R5129:Fstl4 UTSW 11 53,077,266 (GRCm39) missense probably damaging 1.00
R5499:Fstl4 UTSW 11 52,959,374 (GRCm39) missense probably benign 0.01
R5578:Fstl4 UTSW 11 53,056,608 (GRCm39) missense probably damaging 1.00
R5715:Fstl4 UTSW 11 52,891,243 (GRCm39) missense possibly damaging 0.53
R6125:Fstl4 UTSW 11 53,077,130 (GRCm39) missense probably benign
R6177:Fstl4 UTSW 11 53,059,031 (GRCm39) missense probably benign 0.00
R6236:Fstl4 UTSW 11 53,077,162 (GRCm39) missense probably benign 0.00
R6311:Fstl4 UTSW 11 53,067,804 (GRCm39) missense probably damaging 1.00
R6611:Fstl4 UTSW 11 53,077,552 (GRCm39) missense probably benign 0.01
R6886:Fstl4 UTSW 11 53,077,277 (GRCm39) missense probably damaging 1.00
R7404:Fstl4 UTSW 11 53,024,898 (GRCm39) missense probably benign 0.03
R7423:Fstl4 UTSW 11 52,959,382 (GRCm39) missense possibly damaging 0.54
R7586:Fstl4 UTSW 11 52,963,256 (GRCm39) missense probably benign 0.00
R7756:Fstl4 UTSW 11 53,059,123 (GRCm39) missense possibly damaging 0.69
R7758:Fstl4 UTSW 11 53,059,123 (GRCm39) missense possibly damaging 0.69
R7775:Fstl4 UTSW 11 53,067,798 (GRCm39) nonsense probably null
R7953:Fstl4 UTSW 11 52,891,050 (GRCm39) missense probably benign 0.01
R8026:Fstl4 UTSW 11 52,959,496 (GRCm39) missense probably damaging 0.99
R8043:Fstl4 UTSW 11 52,891,050 (GRCm39) missense probably benign 0.01
R8375:Fstl4 UTSW 11 53,053,502 (GRCm39) missense possibly damaging 0.63
R8866:Fstl4 UTSW 11 52,963,233 (GRCm39) missense possibly damaging 0.54
R9103:Fstl4 UTSW 11 52,664,696 (GRCm39) missense probably benign 0.21
R9182:Fstl4 UTSW 11 53,024,905 (GRCm39) missense probably damaging 0.98
R9297:Fstl4 UTSW 11 53,024,973 (GRCm39) missense possibly damaging 0.50
R9390:Fstl4 UTSW 11 52,891,102 (GRCm39) missense probably benign
R9396:Fstl4 UTSW 11 52,664,778 (GRCm39) missense probably benign
R9506:Fstl4 UTSW 11 53,024,950 (GRCm39) missense probably benign 0.18
R9518:Fstl4 UTSW 11 53,056,647 (GRCm39) missense possibly damaging 0.71
R9523:Fstl4 UTSW 11 53,075,466 (GRCm39) missense probably benign 0.04
R9586:Fstl4 UTSW 11 53,077,729 (GRCm39) missense probably benign 0.02
R9594:Fstl4 UTSW 11 52,664,694 (GRCm39) missense probably benign
X0013:Fstl4 UTSW 11 53,053,446 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCTACATGACTGAAGGGTTGGG -3'
(R):5'- TTGGAGACACTGACGATGAAGC -3'

Sequencing Primer
(F):5'- GGTTGGGAAGCCATCATTTAAAAC -3'
(R):5'- ACGATGAAGCGTCCGTCAG -3'
Posted On 2022-06-15