Mutagenetix > Incidental Mutation

Incidental Mutation 'R9452:Micall2'

714324

Institutional Source

Beutler Lab

Gene Symbol

Micall2

Ensembl Gene

ENSMUSG00000036718

Gene Name

MICAL-like 2

Synonyms

MICAL-L2, Jrab, A930021H16Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.352) question?

Stock #

R9452 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

139692451-139722091 bp(-) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

T to G at 139703311 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000039707 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044642] [ENSMUST00000170773]

AlphaFold

Q3TN34

Predicted Effect

probably null
Transcript: ENSMUST00000044642

SMART Domains

Protein: ENSMUSP00000039707
Gene: ENSMUSG00000036718

Domain	Start	End	E-Value	Type
CH	3	102	4.34e-20	SMART
LIM	187	241	1.62e-5	SMART
low complexity region	242	253	N/A	INTRINSIC
low complexity region	326	344	N/A	INTRINSIC
low complexity region	428	489	N/A	INTRINSIC
low complexity region	502	518	N/A	INTRINSIC
low complexity region	728	746	N/A	INTRINSIC
low complexity region	755	770	N/A	INTRINSIC
DUF3585	840	980	3.1e-63	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000170773

SMART Domains

Protein: ENSMUSP00000127937
Gene: ENSMUSG00000036718

Domain	Start	End	E-Value	Type
SCOP:d1bkra_	1	25	9e-5	SMART
Blast:DUF3585	1	45	2e-7	BLAST
LIM	104	158	1.62e-5	SMART
low complexity region	159	170	N/A	INTRINSIC
low complexity region	243	261	N/A	INTRINSIC
low complexity region	345	406	N/A	INTRINSIC
low complexity region	419	435	N/A	INTRINSIC
low complexity region	645	663	N/A	INTRINSIC
low complexity region	672	687	N/A	INTRINSIC
DUF3585	757	897	3.1e-63	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb8	A	G	5: 24,612,382 (GRCm39)	Y564C	probably null	Het
Ablim2	A	T	5: 36,015,198 (GRCm39)	D470V	probably benign	Het
Actr10	T	C	12: 71,006,818 (GRCm39)		probably null	Het
Adam4	T	A	12: 81,467,071 (GRCm39)	I517L	probably benign	Het
Ank1	C	A	8: 23,622,429 (GRCm39)	D1659E	probably benign	Het
Arnt2	A	G	7: 83,933,334 (GRCm39)	V308A	probably benign	Het
Avl9	T	C	6: 56,706,726 (GRCm39)	V184A	probably damaging	Het
Bag1	A	T	4: 40,947,733 (GRCm39)	I157N	probably benign	Het
Bicc1	A	T	10: 70,792,981 (GRCm39)	D224E	probably damaging	Het
C2	T	G	17: 35,095,319 (GRCm39)	I209L	probably benign	Het
Cacna1e	A	T	1: 154,289,720 (GRCm39)		probably null	Het
Capn1	T	C	19: 6,057,287 (GRCm39)	Y387C	probably damaging	Het
Cebpa	A	G	7: 34,819,033 (GRCm39)	I64V	possibly damaging	Het
Celf4	T	G	18: 25,624,219 (GRCm39)	M436L	probably benign	Het
Cfb	C	A	17: 35,078,084 (GRCm39)	V436L	probably benign	Het
Ciart	C	T	3: 95,788,527 (GRCm39)	C104Y	probably benign	Het
Cmya5	A	G	13: 93,232,394 (GRCm39)	L898P	probably benign	Het
Cyp2c68	A	T	19: 39,687,833 (GRCm39)	M388K	possibly damaging	Het
Cypt12	T	C	3: 18,002,843 (GRCm39)	V72A	probably benign	Het
Dlec1	A	G	9: 118,941,541 (GRCm39)	D309G	probably benign	Het
Dnah1	A	G	14: 31,018,448 (GRCm39)	V1394A	probably benign	Het
Dpp3	A	C	19: 4,973,750 (GRCm39)	S108A	probably benign	Het
Eif5b	C	T	1: 38,084,861 (GRCm39)	P903S	probably damaging	Het
Erc2	A	G	14: 27,733,690 (GRCm39)	D580G	probably damaging	Het
Fam98c	A	G	7: 28,852,901 (GRCm39)	V44A	probably benign	Het
Fer	A	G	17: 64,231,067 (GRCm39)	K273E	probably benign	Het
Gzme	T	A	14: 56,355,854 (GRCm39)	I153F	probably benign	Het
Ice1	A	T	13: 70,744,462 (GRCm39)	N2040K	probably damaging	Het
Ifi214	G	T	1: 173,356,894 (GRCm39)	Q70K	possibly damaging	Het
Itpripl1	C	A	2: 126,983,334 (GRCm39)	E263*	probably null	Het
Kat8	A	G	7: 127,524,421 (GRCm39)	K433R	probably benign	Het
Klra5	C	A	6: 129,883,686 (GRCm39)	W147L	possibly damaging	Het
Lefty1	A	T	1: 180,762,849 (GRCm39)	T59S	probably benign	Het
Lig3	C	T	11: 82,681,448 (GRCm39)	T481M	probably damaging	Het
Lrfn1	A	T	7: 28,159,157 (GRCm39)	I359F	probably damaging	Het
Lrp1b	A	T	2: 41,850,726 (GRCm39)	C90*	probably null	Het
Mtmr3	G	T	11: 4,441,037 (GRCm39)	L539I	probably damaging	Het
Obscn	C	A	11: 58,924,962 (GRCm39)		probably null	Het
Olfml3	T	C	3: 103,643,575 (GRCm39)	T269A	probably benign	Het
Oprl1	C	T	2: 181,360,454 (GRCm39)	A170V	possibly damaging	Het
Or4c112	C	T	2: 88,854,234 (GRCm39)	G38R		Het
Pdgfrb	G	A	18: 61,198,798 (GRCm39)	V319M	possibly damaging	Het
Pirb	G	A	7: 3,720,617 (GRCm39)	R294C	possibly damaging	Het
Piwil1	T	C	5: 128,824,957 (GRCm39)	S552P	probably benign	Het
Pkd2	T	C	5: 104,614,841 (GRCm39)	F228L	probably damaging	Het
Pkhd1	T	A	1: 20,682,953 (GRCm39)	D83V	possibly damaging	Het
Plcl1	A	G	1: 55,734,992 (GRCm39)	D111G	probably damaging	Het
Plpp2	A	G	10: 79,363,702 (GRCm39)	S162P	probably damaging	Het
Prex2	T	A	1: 11,256,151 (GRCm39)	I1174K	probably benign	Het
Prkdc	T	A	16: 15,485,465 (GRCm39)	Y431N	possibly damaging	Het
Pter	A	G	2: 12,985,111 (GRCm39)	D147G	probably benign	Het
Rbsn	T	C	6: 92,178,745 (GRCm39)	D134G	possibly damaging	Het
Rnf220	T	C	4: 117,153,339 (GRCm39)	D299G	probably benign	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,133 (GRCm39)		probably benign	Het
Scn2a	G	A	2: 65,595,163 (GRCm39)	S2004N	probably benign	Het
Slc7a4	T	A	16: 17,391,271 (GRCm39)	N593I	probably damaging	Het
Spata31e3	T	C	13: 50,400,808 (GRCm39)	H506R	possibly damaging	Het
Speg	T	C	1: 75,399,152 (GRCm39)	S2200P	probably benign	Het
Thoc2l	T	A	5: 104,669,610 (GRCm39)	N1377K	probably benign	Het
Tle1	A	T	4: 72,040,596 (GRCm39)	H654Q	probably benign	Het
Ttll6	G	A	11: 96,026,588 (GRCm39)	R124H	probably damaging	Het
Vmn2r111	A	G	17: 22,778,132 (GRCm39)	C516R	probably damaging	Het
Vmn2r89	T	C	14: 51,693,288 (GRCm39)	S213P	probably damaging	Het
Vmn2r-ps158	A	T	7: 42,697,257 (GRCm39)	L771F	possibly damaging	Het
Washc4	A	G	10: 83,396,387 (GRCm39)	N399S	probably benign	Het
Wdr37	G	A	13: 8,897,663 (GRCm39)	H198Y		Het
Zfp407	A	G	18: 84,580,579 (GRCm39)	L178P	probably benign	Het
Zfp882	C	T	8: 72,668,831 (GRCm39)	H553Y	probably damaging	Het
Zfp956	T	C	6: 47,940,370 (GRCm39)	V243A	probably benign	Het

Other mutations in Micall2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Micall2	APN	5	139,703,311 (GRCm39)	critical splice acceptor site	probably null
IGL00496:Micall2	APN	5	139,702,083 (GRCm39)	missense	probably benign	0.01
IGL02641:Micall2	APN	5	139,705,094 (GRCm39)	missense	probably damaging	1.00
IGL03245:Micall2	APN	5	139,705,014 (GRCm39)	missense	probably damaging	1.00
IGL03252:Micall2	APN	5	139,702,481 (GRCm39)	missense	probably benign	0.01
R1214:Micall2	UTSW	5	139,697,396 (GRCm39)	missense	probably damaging	0.97
R1468:Micall2	UTSW	5	139,705,097 (GRCm39)	missense	probably damaging	1.00
R1468:Micall2	UTSW	5	139,705,097 (GRCm39)	missense	probably damaging	1.00
R1831:Micall2	UTSW	5	139,702,508 (GRCm39)	missense	probably benign	0.09
R1833:Micall2	UTSW	5	139,702,508 (GRCm39)	missense	probably benign	0.09
R1969:Micall2	UTSW	5	139,721,885 (GRCm39)	missense	probably damaging	1.00
R2023:Micall2	UTSW	5	139,703,266 (GRCm39)	missense	possibly damaging	0.51
R2060:Micall2	UTSW	5	139,697,317 (GRCm39)	missense	probably damaging	0.98
R2330:Micall2	UTSW	5	139,703,270 (GRCm39)	missense	probably damaging	1.00
R3820:Micall2	UTSW	5	139,701,611 (GRCm39)	missense	possibly damaging	0.92
R4299:Micall2	UTSW	5	139,695,226 (GRCm39)	intron	probably benign
R4334:Micall2	UTSW	5	139,699,105 (GRCm39)	missense	probably damaging	1.00
R4451:Micall2	UTSW	5	139,692,852 (GRCm39)	missense	probably damaging	1.00
R4769:Micall2	UTSW	5	139,692,641 (GRCm39)	missense	probably damaging	0.97
R4911:Micall2	UTSW	5	139,702,580 (GRCm39)	missense	probably damaging	1.00
R4996:Micall2	UTSW	5	139,696,344 (GRCm39)	missense	probably benign	0.31
R5118:Micall2	UTSW	5	139,702,202 (GRCm39)	missense	probably damaging	1.00
R5155:Micall2	UTSW	5	139,695,986 (GRCm39)	missense	probably damaging	1.00
R5475:Micall2	UTSW	5	139,702,224 (GRCm39)	missense	probably damaging	1.00
R5750:Micall2	UTSW	5	139,701,456 (GRCm39)	splice site	probably null
R5998:Micall2	UTSW	5	139,692,666 (GRCm39)	critical splice acceptor site	probably null
R6181:Micall2	UTSW	5	139,702,506 (GRCm39)	missense	probably benign	0.41
R6852:Micall2	UTSW	5	139,701,548 (GRCm39)	missense	possibly damaging	0.90
R7046:Micall2	UTSW	5	139,694,699 (GRCm39)	unclassified	probably benign
R7395:Micall2	UTSW	5	139,702,124 (GRCm39)	missense	possibly damaging	0.78
R8514:Micall2	UTSW	5	139,701,977 (GRCm39)	missense	probably damaging	1.00
R8889:Micall2	UTSW	5	139,703,254 (GRCm39)	missense	probably damaging	0.98
R8892:Micall2	UTSW	5	139,703,254 (GRCm39)	missense	probably damaging	0.98
R8960:Micall2	UTSW	5	139,702,025 (GRCm39)	missense	probably benign	0.23
R9060:Micall2	UTSW	5	139,705,035 (GRCm39)	missense	probably damaging	1.00
R9209:Micall2	UTSW	5	139,696,170 (GRCm39)	missense	unknown
R9227:Micall2	UTSW	5	139,701,827 (GRCm39)	missense	unknown
R9230:Micall2	UTSW	5	139,701,827 (GRCm39)	missense	unknown
R9260:Micall2	UTSW	5	139,695,453 (GRCm39)	missense	unknown
Z1088:Micall2	UTSW	5	139,702,050 (GRCm39)	missense	probably benign	0.12
Z1088:Micall2	UTSW	5	139,692,649 (GRCm39)	missense	probably damaging	1.00
Z1177:Micall2	UTSW	5	139,696,057 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCACCAATGGAAGACGGATG -3'
(R):5'- CCATGAGTTGCCAGCATCTC -3'

Sequencing Primer
(F):5'- ACGGATGGGAGGGCCAC -3'
(R):5'- CAGTCTCCTTCTTTCCAACGAGG -3'

Posted On

2022-06-15