Mutagenetix > Incidental Mutation

Incidental Mutation 'R9455:Fat4'

714517

Institutional Source

Beutler Lab

Gene Symbol

Fat4

Ensembl Gene

ENSMUSG00000046743

Gene Name

FAT atypical cadherin 4

Synonyms

6030410K14Rik

MMRRC Submission

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9455 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

38941089-39066134 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 38945412 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 1435 (L1435P)

Ref Sequence

ENSEMBL: ENSMUSP00000061836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061260]

AlphaFold

no structure available at present

Predicted Effect

SMART Domains

Protein: ENSMUSP00000061836
Gene: ENSMUSG00000046743
AA Change: L1435P

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
CA	60	133	4.09e-7	SMART
CA	157	248	4.51e-18	SMART
CA	272	351	7.66e-30	SMART
CA	380	473	2.55e-17	SMART
CA	497	580	8.27e-26	SMART
CA	605	687	6.46e-28	SMART
CA	711	791	1e-24	SMART
CA	815	891	3.78e-20	SMART
CA	915	994	8.6e-24	SMART
CA	1018	1098	7.09e-25	SMART
CA	1122	1208	6.78e-22	SMART
CA	1232	1313	2.63e-28	SMART
CA	1337	1418	7.25e-31	SMART
CA	1442	1527	4.58e-19	SMART
CA	1550	1629	4.52e-9	SMART
CA	1651	1738	1.3e-9	SMART
CA	1762	1839	2.01e-24	SMART
CA	1863	1942	3.11e-21	SMART
CA	1966	2049	5.85e-26	SMART
CA	2072	2152	1.88e-29	SMART
CA	2176	2257	3.06e-29	SMART
CA	2282	2362	2.61e-23	SMART
CA	2386	2466	2.99e-32	SMART
CA	2490	2568	9.92e-6	SMART
CA	2588	2669	6.58e-20	SMART
CA	2692	2773	7.25e-31	SMART
CA	2796	2872	1.69e-22	SMART
CA	2896	2983	3.16e-22	SMART
CA	3007	3089	1.01e-15	SMART
CA	3113	3194	1.25e-25	SMART
CA	3218	3298	7e-15	SMART
CA	3322	3405	3.96e-14	SMART
CA	3428	3510	3.41e-27	SMART
CA	3532	3614	5.64e-19	SMART
EGF	3807	3862	1.78e-2	SMART
EGF_CA	3864	3900	2.36e-16	SMART
EGF_CA	3902	3938	7.99e-14	SMART
EGF	3943	3976	1.24e-1	SMART
LamG	3996	4144	4.08e-19	SMART
EGF	4167	4200	5.88e-3	SMART
LamG	4244	4375	1.76e-23	SMART
EGF	4430	4464	1.41e-5	SMART
low complexity region	4514	4526	N/A	INTRINSIC
low complexity region	4533	4550	N/A	INTRINSIC
low complexity region	4840	4849	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous inactivation of this gene leads to neonatal lethality, reduced birth body size, curly tails, kyphosis, small lungs, renal cysts, and defects in sternum and vertebrae morphology, neural tube width, cochlear elongation, stereocilia orientation, kidney development, and intestinal elongation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	C	11: 9,353,897 (GRCm39)	Y3740H	probably damaging	Het
Acsl6	G	T	11: 54,210,752 (GRCm39)		probably benign	Het
Adamts5	C	T	16: 85,667,017 (GRCm39)	D492N	probably damaging	Het
Als2	T	C	1: 59,219,296 (GRCm39)	E1218G	probably damaging	Het
Bcl2l15	C	T	3: 103,743,369 (GRCm39)	T90I	unknown	Het
Bsn	A	G	9: 107,988,531 (GRCm39)	V2407A	unknown	Het
Casp8ap2	T	C	4: 32,643,924 (GRCm39)	M999T	possibly damaging	Het
Ccdc66	G	A	14: 27,208,872 (GRCm39)	P461S	probably benign	Het
Cd46	G	A	1: 194,744,704 (GRCm39)	L345F	possibly damaging	Het
Cep295	A	T	9: 15,245,046 (GRCm39)	S1137T	possibly damaging	Het
Chd7	A	G	4: 8,752,061 (GRCm39)	Y186C	unknown	Het
Clca3b	T	A	3: 144,529,023 (GRCm39)	T884S	unknown	Het
Cndp2	T	A	18: 84,690,246 (GRCm39)	Y232F	probably benign	Het
Cx3cr1	A	T	9: 119,880,659 (GRCm39)	Y248N	probably damaging	Het
Dnm3	A	T	1: 162,148,524 (GRCm39)	N282K	possibly damaging	Het
Emilin2	T	C	17: 71,581,485 (GRCm39)	I414V	probably benign	Het
F3	A	T	3: 121,527,866 (GRCm39)	N288I	probably damaging	Het
Fbxw14	A	T	9: 109,103,567 (GRCm39)	N371K	probably benign	Het
Firrm	G	T	1: 163,781,721 (GRCm39)	T902K	probably benign	Het
Fn1	T	A	1: 71,647,112 (GRCm39)	E1559D	probably benign	Het
Frrs1	T	A	3: 116,695,972 (GRCm39)	M497K	possibly damaging	Het
Gm45861	C	T	8: 28,041,394 (GRCm39)	Q1000*	probably null	Het
Gpr152	T	C	19: 4,193,844 (GRCm39)	S462P	probably benign	Het
Irgq	A	G	7: 24,231,217 (GRCm39)	N136S	probably benign	Het
Kdm2b	T	C	5: 123,099,537 (GRCm39)	Y134C	probably damaging	Het
Klf12	A	T	14: 100,347,226 (GRCm39)	L35H	probably damaging	Het
Krt9	TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC	TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC	11: 100,079,903 (GRCm39)		probably benign	Het
Ksr1	A	T	11: 78,911,602 (GRCm39)	C727S	possibly damaging	Het
Lpin3	G	T	2: 160,737,259 (GRCm39)	R102L	probably benign	Het
Ltv1	T	C	10: 13,058,117 (GRCm39)	E229G	probably damaging	Het
Ly6f	T	A	15: 75,141,648 (GRCm39)	Y30*	probably null	Het
Map2k7	C	G	8: 4,293,957 (GRCm39)	R168G	probably damaging	Het
Mep1a	T	C	17: 43,805,867 (GRCm39)	E100G	probably benign	Het
Mrgpra6	T	A	7: 46,838,967 (GRCm39)	Y46F	probably damaging	Het
Mroh6	C	A	15: 75,759,905 (GRCm39)	R143L	probably benign	Het
Myom2	T	A	8: 15,156,293 (GRCm39)	H713Q	probably benign	Het
Ndn	C	T	7: 61,998,337 (GRCm39)	P61L	possibly damaging	Het
Or51b6	G	T	7: 103,556,200 (GRCm39)	V182F		Het
Pear1	T	A	3: 87,666,488 (GRCm39)	T72S	possibly damaging	Het
Sel1l3	T	A	5: 53,289,157 (GRCm39)	N888I	probably damaging	Het
Sell	A	T	1: 163,894,218 (GRCm39)	T171S	probably benign	Het
Semp2l1	A	T	1: 32,585,907 (GRCm39)	M1K	probably null	Het
Sin3b	G	T	8: 73,450,681 (GRCm39)	A37S	possibly damaging	Het
Sipa1l1	T	C	12: 82,434,399 (GRCm39)	F723S	probably damaging	Het
Slc26a8	T	C	17: 28,863,588 (GRCm39)	T724A	probably damaging	Het
Slc51a	T	G	16: 32,305,013 (GRCm39)	N25H	probably damaging	Het
Slc9a4	T	C	1: 40,668,612 (GRCm39)	F752L	probably benign	Het
Slco4a1	A	C	2: 180,115,370 (GRCm39)	D649A	probably benign	Het
Tax1bp1	T	A	6: 52,743,029 (GRCm39)	H805Q	probably damaging	Het
Tgfb1i1	T	C	7: 127,852,009 (GRCm39)	F303L	probably damaging	Het
Tsg101	A	T	7: 46,563,151 (GRCm39)	D34E	probably damaging	Het
Tspoap1	T	C	11: 87,661,359 (GRCm39)	S604P	probably damaging	Het
Ttll2	C	A	17: 7,619,692 (GRCm39)	W78C	probably damaging	Het
Ttn	A	G	2: 76,749,334 (GRCm39)	F3905S	possibly damaging	Het
Txnrd2	G	T	16: 18,248,615 (GRCm39)	C57F	probably damaging	Het
Vcan	A	T	13: 89,837,452 (GRCm39)	D2697E	probably damaging	Het
Vwa8	G	A	14: 79,300,115 (GRCm39)	V983I	probably damaging	Het

Other mutations in Fat4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Fat4	APN	3	39,036,398 (GRCm39)	missense	probably damaging	1.00
IGL00509:Fat4	APN	3	38,943,188 (GRCm39)	missense	probably damaging	1.00
IGL00698:Fat4	APN	3	39,035,294 (GRCm39)	missense	probably benign	0.17
IGL00934:Fat4	APN	3	38,944,822 (GRCm39)	missense	probably damaging	1.00
IGL01063:Fat4	APN	3	38,944,728 (GRCm39)	missense	possibly damaging	0.80
IGL01123:Fat4	APN	3	39,011,418 (GRCm39)	missense	probably benign	0.00
IGL01313:Fat4	APN	3	39,061,350 (GRCm39)	missense	possibly damaging	0.53
IGL01328:Fat4	APN	3	39,034,807 (GRCm39)	missense	probably damaging	1.00
IGL01328:Fat4	APN	3	38,944,140 (GRCm39)	missense	probably damaging	1.00
IGL01374:Fat4	APN	3	38,941,647 (GRCm39)	missense	probably damaging	1.00
IGL01412:Fat4	APN	3	38,945,330 (GRCm39)	missense	probably benign	0.09
IGL01472:Fat4	APN	3	38,942,219 (GRCm39)	missense	probably damaging	1.00
IGL01514:Fat4	APN	3	39,003,683 (GRCm39)	missense	possibly damaging	0.89
IGL01548:Fat4	APN	3	39,063,406 (GRCm39)	missense	probably damaging	1.00
IGL01548:Fat4	APN	3	38,941,907 (GRCm39)	missense	probably damaging	0.99
IGL01576:Fat4	APN	3	38,943,096 (GRCm39)	missense	probably damaging	1.00
IGL01591:Fat4	APN	3	39,064,524 (GRCm39)	nonsense	probably null
IGL01626:Fat4	APN	3	39,005,181 (GRCm39)	missense	probably damaging	1.00
IGL01746:Fat4	APN	3	39,045,880 (GRCm39)	nonsense	probably null
IGL01800:Fat4	APN	3	39,035,878 (GRCm39)	missense	probably damaging	0.99
IGL01815:Fat4	APN	3	38,942,922 (GRCm39)	missense	probably damaging	1.00
IGL01863:Fat4	APN	3	39,024,768 (GRCm39)	splice site	probably benign
IGL01917:Fat4	APN	3	38,943,879 (GRCm39)	missense	possibly damaging	0.89
IGL01936:Fat4	APN	3	39,033,923 (GRCm39)	missense	probably benign	0.10
IGL02060:Fat4	APN	3	39,064,420 (GRCm39)	missense	probably damaging	1.00
IGL02103:Fat4	APN	3	38,943,348 (GRCm39)	missense	probably damaging	0.97
IGL02119:Fat4	APN	3	39,037,088 (GRCm39)	missense	probably benign	0.10
IGL02124:Fat4	APN	3	38,942,553 (GRCm39)	missense	probably damaging	1.00
IGL02164:Fat4	APN	3	39,050,354 (GRCm39)	critical splice donor site	probably null
IGL02182:Fat4	APN	3	38,944,695 (GRCm39)	missense	probably damaging	1.00
IGL02207:Fat4	APN	3	39,005,412 (GRCm39)	missense	probably benign	0.16
IGL02210:Fat4	APN	3	38,946,002 (GRCm39)	missense	probably benign	0.01
IGL02257:Fat4	APN	3	39,055,288 (GRCm39)	missense	probably benign	0.09
IGL02271:Fat4	APN	3	39,034,068 (GRCm39)	missense	probably benign	0.18
IGL02305:Fat4	APN	3	39,064,137 (GRCm39)	missense	probably damaging	1.00
IGL02314:Fat4	APN	3	38,941,779 (GRCm39)	missense	probably damaging	1.00
IGL02455:Fat4	APN	3	39,005,280 (GRCm39)	missense	possibly damaging	0.48
IGL02468:Fat4	APN	3	39,037,195 (GRCm39)	missense	probably benign
IGL02478:Fat4	APN	3	38,942,364 (GRCm39)	missense	probably damaging	1.00
IGL02480:Fat4	APN	3	39,064,579 (GRCm39)	missense	probably damaging	1.00
IGL02487:Fat4	APN	3	38,941,394 (GRCm39)	missense	probably damaging	1.00
IGL02632:Fat4	APN	3	39,056,913 (GRCm39)	missense	probably benign	0.04
IGL02665:Fat4	APN	3	39,056,985 (GRCm39)	missense	probably benign	0.08
IGL02674:Fat4	APN	3	39,037,486 (GRCm39)	missense	probably benign	0.35
IGL02692:Fat4	APN	3	39,005,235 (GRCm39)	missense	probably damaging	1.00
IGL02710:Fat4	APN	3	38,944,744 (GRCm39)	missense	probably damaging	1.00
IGL02803:Fat4	APN	3	38,943,444 (GRCm39)	missense	probably damaging	1.00
IGL02834:Fat4	APN	3	39,010,893 (GRCm39)	missense	probably damaging	1.00
IGL02891:Fat4	APN	3	39,005,422 (GRCm39)	missense	probably damaging	1.00
IGL02982:Fat4	APN	3	38,944,992 (GRCm39)	missense	probably damaging	1.00
IGL02993:Fat4	APN	3	39,011,304 (GRCm39)	missense	probably damaging	1.00
IGL02996:Fat4	APN	3	39,012,674 (GRCm39)	missense	probably damaging	1.00
IGL03029:Fat4	APN	3	39,036,740 (GRCm39)	missense	possibly damaging	0.46
IGL03124:Fat4	APN	3	39,035,701 (GRCm39)	missense	possibly damaging	0.61
IGL03144:Fat4	APN	3	39,011,008 (GRCm39)	missense	possibly damaging	0.68
IGL03149:Fat4	APN	3	39,045,834 (GRCm39)	missense	probably damaging	1.00
IGL03169:Fat4	APN	3	39,011,547 (GRCm39)	missense	probably benign	0.02
IGL03190:Fat4	APN	3	39,035,390 (GRCm39)	missense	probably damaging	1.00
IGL03272:Fat4	APN	3	39,063,852 (GRCm39)	missense	probably benign
IGL03371:Fat4	APN	3	39,037,336 (GRCm39)	missense	possibly damaging	0.65
IGL03372:Fat4	APN	3	38,943,283 (GRCm39)	missense	possibly damaging	0.88
IGL03388:Fat4	APN	3	39,011,376 (GRCm39)	missense	probably damaging	1.00
IGL03394:Fat4	APN	3	38,946,168 (GRCm39)	missense	probably damaging	0.99
IGL03394:Fat4	APN	3	39,063,513 (GRCm39)	missense	probably damaging	1.00
IGL03405:Fat4	APN	3	39,012,599 (GRCm39)	missense	probably benign	0.02
IGL03410:Fat4	APN	3	38,945,325 (GRCm39)	missense	probably damaging	1.00
Asahi	UTSW	3	39,035,968 (GRCm39)	missense	probably damaging	1.00
Expulsion	UTSW	3	38,943,798 (GRCm39)	missense	probably benign	0.00
heineken	UTSW	3	39,034,529 (GRCm39)	missense	probably damaging	1.00
schlitz	UTSW	3	39,034,808 (GRCm39)	missense	probably damaging	1.00
PIT4696001:Fat4	UTSW	3	39,036,506 (GRCm39)	missense	probably damaging	0.98
PIT4696001:Fat4	UTSW	3	38,943,153 (GRCm39)	missense	probably benign	0.04
R0015:Fat4	UTSW	3	39,036,652 (GRCm39)	missense	probably damaging	1.00
R0015:Fat4	UTSW	3	39,036,652 (GRCm39)	missense	probably damaging	1.00
R0078:Fat4	UTSW	3	38,943,080 (GRCm39)	missense	probably benign	0.35
R0100:Fat4	UTSW	3	39,034,397 (GRCm39)	missense	probably damaging	1.00
R0100:Fat4	UTSW	3	39,034,397 (GRCm39)	missense	probably damaging	1.00
R0201:Fat4	UTSW	3	38,945,745 (GRCm39)	missense	probably damaging	0.99
R0280:Fat4	UTSW	3	38,944,965 (GRCm39)	missense	probably benign
R0357:Fat4	UTSW	3	38,945,376 (GRCm39)	missense	probably damaging	1.00
R0409:Fat4	UTSW	3	39,031,562 (GRCm39)	missense	probably damaging	1.00
R0498:Fat4	UTSW	3	39,034,786 (GRCm39)	missense	probably benign	0.00
R0502:Fat4	UTSW	3	39,057,073 (GRCm39)	missense	probably damaging	0.98
R0506:Fat4	UTSW	3	38,942,463 (GRCm39)	missense	probably benign	0.00
R0532:Fat4	UTSW	3	39,035,870 (GRCm39)	missense	probably benign	0.02
R0616:Fat4	UTSW	3	38,997,019 (GRCm39)	missense	probably damaging	1.00
R0630:Fat4	UTSW	3	39,054,321 (GRCm39)	missense	probably damaging	1.00
R0678:Fat4	UTSW	3	38,943,843 (GRCm39)	missense	probably damaging	1.00
R0685:Fat4	UTSW	3	39,055,327 (GRCm39)	missense	probably benign
R0729:Fat4	UTSW	3	39,054,444 (GRCm39)	splice site	probably benign
R0748:Fat4	UTSW	3	38,941,977 (GRCm39)	missense	possibly damaging	0.67
R0811:Fat4	UTSW	3	39,011,623 (GRCm39)	missense	probably damaging	1.00
R0812:Fat4	UTSW	3	39,011,623 (GRCm39)	missense	probably damaging	1.00
R0830:Fat4	UTSW	3	39,053,258 (GRCm39)	missense	probably benign	0.26
R0841:Fat4	UTSW	3	39,050,147 (GRCm39)	missense	probably damaging	0.99
R0884:Fat4	UTSW	3	39,037,007 (GRCm39)	missense	possibly damaging	0.89
R1056:Fat4	UTSW	3	38,945,541 (GRCm39)	missense	probably damaging	1.00
R1066:Fat4	UTSW	3	39,011,376 (GRCm39)	missense	probably damaging	1.00
R1078:Fat4	UTSW	3	39,037,235 (GRCm39)	missense	probably benign	0.10
R1084:Fat4	UTSW	3	39,033,974 (GRCm39)	missense	possibly damaging	0.88
R1118:Fat4	UTSW	3	39,037,091 (GRCm39)	missense	possibly damaging	0.88
R1213:Fat4	UTSW	3	38,944,520 (GRCm39)	missense	probably benign	0.01
R1418:Fat4	UTSW	3	38,944,962 (GRCm39)	missense	probably damaging	1.00
R1475:Fat4	UTSW	3	38,942,472 (GRCm39)	missense	probably damaging	1.00
R1487:Fat4	UTSW	3	39,050,066 (GRCm39)	missense	possibly damaging	0.77
R1511:Fat4	UTSW	3	39,037,225 (GRCm39)	missense	probably damaging	0.97
R1534:Fat4	UTSW	3	38,944,238 (GRCm39)	missense	probably damaging	1.00
R1558:Fat4	UTSW	3	38,943,135 (GRCm39)	missense	probably damaging	1.00
R1586:Fat4	UTSW	3	38,943,009 (GRCm39)	missense	probably damaging	1.00
R1592:Fat4	UTSW	3	39,061,326 (GRCm39)	missense	probably damaging	0.99
R1655:Fat4	UTSW	3	39,011,467 (GRCm39)	missense	probably damaging	0.97
R1662:Fat4	UTSW	3	39,034,928 (GRCm39)	missense	probably damaging	1.00
R1710:Fat4	UTSW	3	39,005,304 (GRCm39)	missense	probably damaging	1.00
R1731:Fat4	UTSW	3	38,945,459 (GRCm39)	missense	probably damaging	1.00
R1761:Fat4	UTSW	3	38,941,638 (GRCm39)	missense	possibly damaging	0.61
R1770:Fat4	UTSW	3	39,064,417 (GRCm39)	missense	probably damaging	1.00
R1828:Fat4	UTSW	3	39,037,607 (GRCm39)	missense	probably damaging	1.00
R1835:Fat4	UTSW	3	39,037,720 (GRCm39)	missense	probably benign	0.00
R1846:Fat4	UTSW	3	39,036,532 (GRCm39)	missense	probably benign	0.00
R1861:Fat4	UTSW	3	39,064,633 (GRCm39)	missense	probably benign	0.09
R1871:Fat4	UTSW	3	39,035,221 (GRCm39)	missense	possibly damaging	0.63
R1981:Fat4	UTSW	3	39,045,813 (GRCm39)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	39,050,239 (GRCm39)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,941,264 (GRCm39)	missense	probably benign
R2056:Fat4	UTSW	3	38,945,319 (GRCm39)	missense	possibly damaging	0.88
R2058:Fat4	UTSW	3	38,945,319 (GRCm39)	missense	possibly damaging	0.88
R2059:Fat4	UTSW	3	38,945,319 (GRCm39)	missense	possibly damaging	0.88
R2070:Fat4	UTSW	3	39,064,804 (GRCm39)	missense	probably benign	0.00
R2078:Fat4	UTSW	3	38,943,822 (GRCm39)	missense	probably damaging	1.00
R2114:Fat4	UTSW	3	39,035,633 (GRCm39)	missense	probably benign	0.01
R2135:Fat4	UTSW	3	39,034,882 (GRCm39)	missense	probably damaging	0.98
R2152:Fat4	UTSW	3	39,037,544 (GRCm39)	missense	probably damaging	1.00
R2153:Fat4	UTSW	3	39,037,544 (GRCm39)	missense	probably damaging	1.00
R2154:Fat4	UTSW	3	38,941,688 (GRCm39)	missense	probably damaging	1.00
R2196:Fat4	UTSW	3	39,035,566 (GRCm39)	missense	probably benign	0.23
R2211:Fat4	UTSW	3	38,945,676 (GRCm39)	missense	possibly damaging	0.77
R2219:Fat4	UTSW	3	39,064,364 (GRCm39)	missense	probably damaging	1.00
R2247:Fat4	UTSW	3	38,946,198 (GRCm39)	missense	probably damaging	1.00
R2263:Fat4	UTSW	3	38,943,138 (GRCm39)	missense	possibly damaging	0.93
R2264:Fat4	UTSW	3	38,944,571 (GRCm39)	missense	probably benign	0.25
R2274:Fat4	UTSW	3	39,050,048 (GRCm39)	missense	possibly damaging	0.47
R2337:Fat4	UTSW	3	39,034,160 (GRCm39)	missense	probably damaging	1.00
R2343:Fat4	UTSW	3	39,011,254 (GRCm39)	missense	probably damaging	0.97
R2365:Fat4	UTSW	3	39,034,568 (GRCm39)	missense	probably benign
R2412:Fat4	UTSW	3	39,011,221 (GRCm39)	missense	probably benign	0.05
R2883:Fat4	UTSW	3	39,034,953 (GRCm39)	missense	probably damaging	1.00
R2942:Fat4	UTSW	3	39,036,485 (GRCm39)	missense	probably damaging	1.00
R2989:Fat4	UTSW	3	39,061,302 (GRCm39)	missense	probably benign
R3103:Fat4	UTSW	3	38,946,089 (GRCm39)	missense	probably benign	0.03
R3158:Fat4	UTSW	3	38,944,940 (GRCm39)	missense	possibly damaging	0.87
R3800:Fat4	UTSW	3	39,035,423 (GRCm39)	missense	possibly damaging	0.48
R3808:Fat4	UTSW	3	39,036,587 (GRCm39)	missense	possibly damaging	0.52
R3848:Fat4	UTSW	3	39,061,410 (GRCm39)	missense	probably benign	0.10
R3850:Fat4	UTSW	3	39,061,410 (GRCm39)	missense	probably benign	0.10
R3957:Fat4	UTSW	3	39,036,495 (GRCm39)	missense	probably benign
R4065:Fat4	UTSW	3	39,063,346 (GRCm39)	missense	probably benign	0.13
R4078:Fat4	UTSW	3	39,034,169 (GRCm39)	missense	probably damaging	1.00
R4096:Fat4	UTSW	3	38,942,024 (GRCm39)	missense	possibly damaging	0.46
R4161:Fat4	UTSW	3	38,996,958 (GRCm39)	missense	possibly damaging	0.95
R4273:Fat4	UTSW	3	38,945,776 (GRCm39)	missense	probably damaging	1.00
R4285:Fat4	UTSW	3	38,943,320 (GRCm39)	missense	probably benign	0.00
R4288:Fat4	UTSW	3	38,945,912 (GRCm39)	missense	probably damaging	1.00
R4407:Fat4	UTSW	3	39,012,689 (GRCm39)	missense	probably benign	0.05
R4528:Fat4	UTSW	3	38,945,443 (GRCm39)	missense	probably benign	0.01
R4547:Fat4	UTSW	3	39,005,432 (GRCm39)	missense	probably damaging	1.00
R4681:Fat4	UTSW	3	38,941,491 (GRCm39)	missense	probably damaging	1.00
R4826:Fat4	UTSW	3	39,037,106 (GRCm39)	missense	probably damaging	1.00
R4855:Fat4	UTSW	3	38,942,466 (GRCm39)	missense	probably benign
R4871:Fat4	UTSW	3	38,945,754 (GRCm39)	missense	probably damaging	1.00
R4897:Fat4	UTSW	3	39,034,781 (GRCm39)	missense	probably damaging	1.00
R4928:Fat4	UTSW	3	39,064,614 (GRCm39)	missense	probably damaging	1.00
R4932:Fat4	UTSW	3	39,061,352 (GRCm39)	missense	probably benign	0.00
R4941:Fat4	UTSW	3	39,011,601 (GRCm39)	missense	probably damaging	1.00
R4943:Fat4	UTSW	3	39,034,322 (GRCm39)	missense	probably benign	0.19
R4959:Fat4	UTSW	3	39,037,195 (GRCm39)	missense	probably benign	0.00
R4973:Fat4	UTSW	3	39,037,195 (GRCm39)	missense	probably benign	0.00
R5098:Fat4	UTSW	3	38,942,438 (GRCm39)	missense	probably benign	0.34
R5163:Fat4	UTSW	3	39,034,946 (GRCm39)	missense	probably damaging	1.00
R5213:Fat4	UTSW	3	39,034,340 (GRCm39)	missense	possibly damaging	0.56
R5328:Fat4	UTSW	3	39,011,017 (GRCm39)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	39,064,527 (GRCm39)	missense	probably benign	0.44
R5337:Fat4	UTSW	3	38,945,776 (GRCm39)	missense	probably damaging	1.00
R5363:Fat4	UTSW	3	38,942,154 (GRCm39)	missense	probably damaging	1.00
R5380:Fat4	UTSW	3	38,943,013 (GRCm39)	missense	probably damaging	1.00
R5384:Fat4	UTSW	3	39,050,095 (GRCm39)	missense	possibly damaging	0.87
R5422:Fat4	UTSW	3	38,941,394 (GRCm39)	missense	possibly damaging	0.92
R5436:Fat4	UTSW	3	38,945,495 (GRCm39)	missense	probably benign	0.00
R5443:Fat4	UTSW	3	39,064,519 (GRCm39)	missense	probably damaging	1.00
R5501:Fat4	UTSW	3	38,941,364 (GRCm39)	missense	probably benign	0.09
R5571:Fat4	UTSW	3	39,064,423 (GRCm39)	missense	probably damaging	1.00
R5625:Fat4	UTSW	3	38,943,083 (GRCm39)	missense	possibly damaging	0.78
R5652:Fat4	UTSW	3	39,057,117 (GRCm39)	missense	probably damaging	0.99
R5725:Fat4	UTSW	3	38,943,774 (GRCm39)	missense	probably damaging	1.00
R5735:Fat4	UTSW	3	39,003,725 (GRCm39)	missense	probably damaging	1.00
R5739:Fat4	UTSW	3	39,037,283 (GRCm39)	missense	probably benign	0.01
R5766:Fat4	UTSW	3	38,943,617 (GRCm39)	missense	probably damaging	1.00
R5780:Fat4	UTSW	3	39,035,104 (GRCm39)	missense	probably damaging	0.96
R5811:Fat4	UTSW	3	38,945,936 (GRCm39)	missense	probably damaging	1.00
R5829:Fat4	UTSW	3	39,061,454 (GRCm39)	missense	probably damaging	1.00
R5879:Fat4	UTSW	3	38,941,485 (GRCm39)	missense	probably benign
R5933:Fat4	UTSW	3	39,005,524 (GRCm39)	critical splice donor site	probably null
R5938:Fat4	UTSW	3	39,005,388 (GRCm39)	missense	probably damaging	1.00
R5940:Fat4	UTSW	3	38,943,798 (GRCm39)	missense	probably benign	0.00
R5945:Fat4	UTSW	3	39,037,355 (GRCm39)	missense	probably benign	0.19
R5963:Fat4	UTSW	3	39,064,696 (GRCm39)	missense	probably damaging	1.00
R6077:Fat4	UTSW	3	39,056,951 (GRCm39)	missense	probably damaging	1.00
R6158:Fat4	UTSW	3	39,037,411 (GRCm39)	missense	possibly damaging	0.95
R6246:Fat4	UTSW	3	38,945,870 (GRCm39)	missense	probably damaging	1.00
R6253:Fat4	UTSW	3	39,005,505 (GRCm39)	missense	probably damaging	0.99
R6259:Fat4	UTSW	3	39,061,395 (GRCm39)	missense	probably benign	0.18
R6295:Fat4	UTSW	3	39,061,229 (GRCm39)	splice site	probably null
R6387:Fat4	UTSW	3	39,037,934 (GRCm39)	missense	probably damaging	1.00
R6390:Fat4	UTSW	3	39,034,529 (GRCm39)	missense	probably damaging	1.00
R6456:Fat4	UTSW	3	39,038,128 (GRCm39)	missense	possibly damaging	0.90
R6493:Fat4	UTSW	3	38,945,036 (GRCm39)	missense	probably damaging	1.00
R6500:Fat4	UTSW	3	39,035,418 (GRCm39)	nonsense	probably null
R6503:Fat4	UTSW	3	39,036,406 (GRCm39)	missense	probably benign	0.00
R6519:Fat4	UTSW	3	39,057,020 (GRCm39)	missense	probably benign
R6566:Fat4	UTSW	3	39,011,275 (GRCm39)	missense	possibly damaging	0.78
R6576:Fat4	UTSW	3	39,033,839 (GRCm39)	missense	probably benign
R6590:Fat4	UTSW	3	39,037,688 (GRCm39)	missense	probably damaging	1.00
R6658:Fat4	UTSW	3	38,997,077 (GRCm39)	missense	probably benign	0.01
R6662:Fat4	UTSW	3	39,010,970 (GRCm39)	missense	possibly damaging	0.95
R6690:Fat4	UTSW	3	39,037,688 (GRCm39)	missense	probably damaging	1.00
R6807:Fat4	UTSW	3	39,036,589 (GRCm39)	missense	probably benign	0.18
R6823:Fat4	UTSW	3	39,038,088 (GRCm39)	missense	probably benign	0.05
R6824:Fat4	UTSW	3	39,011,674 (GRCm39)	missense	probably benign	0.00
R6830:Fat4	UTSW	3	39,035,966 (GRCm39)	missense	probably benign	0.00
R6925:Fat4	UTSW	3	39,050,353 (GRCm39)	critical splice donor site	probably null
R6948:Fat4	UTSW	3	39,063,595 (GRCm39)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	39,050,120 (GRCm39)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	39,035,924 (GRCm39)	missense	probably damaging	1.00
R7017:Fat4	UTSW	3	38,945,692 (GRCm39)	missense	probably benign
R7030:Fat4	UTSW	3	39,036,107 (GRCm39)	missense	probably damaging	1.00
R7044:Fat4	UTSW	3	39,064,960 (GRCm39)	missense	probably benign	0.02
R7044:Fat4	UTSW	3	39,064,959 (GRCm39)	missense	probably benign
R7045:Fat4	UTSW	3	38,942,750 (GRCm39)	missense	probably benign	0.01
R7094:Fat4	UTSW	3	38,944,023 (GRCm39)	missense	probably damaging	1.00
R7111:Fat4	UTSW	3	39,064,682 (GRCm39)	missense	probably damaging	1.00
R7130:Fat4	UTSW	3	39,034,936 (GRCm39)	missense	probably damaging	0.99
R7168:Fat4	UTSW	3	39,034,808 (GRCm39)	missense	probably damaging	1.00
R7192:Fat4	UTSW	3	39,034,613 (GRCm39)	missense	probably benign	0.04
R7194:Fat4	UTSW	3	39,038,044 (GRCm39)	missense	probably damaging	1.00
R7194:Fat4	UTSW	3	38,943,033 (GRCm39)	missense	probably damaging	1.00
R7199:Fat4	UTSW	3	39,031,511 (GRCm39)	missense	probably damaging	0.98
R7213:Fat4	UTSW	3	39,053,236 (GRCm39)	missense	possibly damaging	0.63
R7216:Fat4	UTSW	3	38,945,192 (GRCm39)	missense	probably damaging	1.00
R7225:Fat4	UTSW	3	39,034,325 (GRCm39)	missense	possibly damaging	0.50
R7238:Fat4	UTSW	3	38,944,562 (GRCm39)	missense	probably benign	0.31
R7239:Fat4	UTSW	3	39,037,989 (GRCm39)	missense	possibly damaging	0.85
R7283:Fat4	UTSW	3	38,943,842 (GRCm39)	missense	probably damaging	1.00
R7296:Fat4	UTSW	3	38,943,294 (GRCm39)	nonsense	probably null
R7372:Fat4	UTSW	3	38,944,358 (GRCm39)	missense	probably damaging	1.00
R7400:Fat4	UTSW	3	38,942,073 (GRCm39)	missense	probably damaging	1.00
R7419:Fat4	UTSW	3	39,054,385 (GRCm39)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	39,063,793 (GRCm39)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	38,941,599 (GRCm39)	missense	probably damaging	0.97
R7431:Fat4	UTSW	3	39,063,306 (GRCm39)	missense	possibly damaging	0.80
R7486:Fat4	UTSW	3	39,011,576 (GRCm39)	nonsense	probably null
R7501:Fat4	UTSW	3	39,012,597 (GRCm39)	nonsense	probably null
R7533:Fat4	UTSW	3	39,061,406 (GRCm39)	missense	probably benign	0.43
R7542:Fat4	UTSW	3	39,035,770 (GRCm39)	missense	possibly damaging	0.64
R7542:Fat4	UTSW	3	39,035,504 (GRCm39)	missense	possibly damaging	0.56
R7548:Fat4	UTSW	3	39,035,263 (GRCm39)	missense	probably benign	0.13
R7567:Fat4	UTSW	3	38,943,485 (GRCm39)	missense	probably damaging	1.00
R7644:Fat4	UTSW	3	39,064,390 (GRCm39)	missense	possibly damaging	0.64
R7660:Fat4	UTSW	3	39,035,309 (GRCm39)	missense	probably benign
R7665:Fat4	UTSW	3	38,943,327 (GRCm39)	missense	probably benign	0.00
R7676:Fat4	UTSW	3	38,945,846 (GRCm39)	missense	probably damaging	0.98
R7832:Fat4	UTSW	3	39,055,353 (GRCm39)	missense	probably benign	0.00
R7848:Fat4	UTSW	3	38,942,000 (GRCm39)	missense	probably benign
R7883:Fat4	UTSW	3	39,035,968 (GRCm39)	missense	probably damaging	1.00
R7892:Fat4	UTSW	3	39,003,588 (GRCm39)	critical splice acceptor site	probably null
R7904:Fat4	UTSW	3	38,941,690 (GRCm39)	missense	probably damaging	1.00
R7952:Fat4	UTSW	3	38,945,870 (GRCm39)	missense	probably damaging	0.98
R8015:Fat4	UTSW	3	39,036,065 (GRCm39)	missense	possibly damaging	0.79
R8040:Fat4	UTSW	3	39,035,815 (GRCm39)	missense	probably damaging	1.00
R8142:Fat4	UTSW	3	38,945,352 (GRCm39)	missense	probably damaging	1.00
R8151:Fat4	UTSW	3	38,946,203 (GRCm39)	missense	probably damaging	0.99
R8163:Fat4	UTSW	3	39,033,881 (GRCm39)	missense	possibly damaging	0.88
R8317:Fat4	UTSW	3	39,012,659 (GRCm39)	missense	possibly damaging	0.80
R8413:Fat4	UTSW	3	39,063,128 (GRCm39)	critical splice acceptor site	probably null
R8447:Fat4	UTSW	3	39,033,824 (GRCm39)	missense	possibly damaging	0.88
R8458:Fat4	UTSW	3	39,035,702 (GRCm39)	missense	probably benign	0.25
R8509:Fat4	UTSW	3	39,036,052 (GRCm39)	missense	probably benign
R8543:Fat4	UTSW	3	39,031,643 (GRCm39)	missense	probably damaging	1.00
R8679:Fat4	UTSW	3	39,064,842 (GRCm39)	missense	probably damaging	1.00
R8726:Fat4	UTSW	3	39,064,647 (GRCm39)	missense	probably damaging	1.00
R8743:Fat4	UTSW	3	38,942,592 (GRCm39)	missense	probably benign	0.16
R8751:Fat4	UTSW	3	38,946,002 (GRCm39)	missense	probably benign	0.01
R8779:Fat4	UTSW	3	39,033,898 (GRCm39)	missense	probably damaging	1.00
R8797:Fat4	UTSW	3	39,053,278 (GRCm39)	missense	probably benign	0.01
R8860:Fat4	UTSW	3	38,946,269 (GRCm39)	missense	probably benign	0.26
R8955:Fat4	UTSW	3	39,037,778 (GRCm39)	missense	probably benign	0.01
R9053:Fat4	UTSW	3	38,941,324 (GRCm39)	nonsense	probably null
R9071:Fat4	UTSW	3	39,037,598 (GRCm39)	missense	probably benign	0.29
R9088:Fat4	UTSW	3	39,061,448 (GRCm39)	missense	probably benign	0.02
R9100:Fat4	UTSW	3	39,064,803 (GRCm39)	missense
R9180:Fat4	UTSW	3	38,942,556 (GRCm39)	missense	possibly damaging	0.78
R9184:Fat4	UTSW	3	39,036,592 (GRCm39)	missense	probably damaging	0.99
R9201:Fat4	UTSW	3	38,945,079 (GRCm39)	missense	probably damaging	1.00
R9206:Fat4	UTSW	3	39,063,390 (GRCm39)	missense	probably damaging	0.99
R9268:Fat4	UTSW	3	38,942,396 (GRCm39)	missense	probably damaging	1.00
R9278:Fat4	UTSW	3	38,945,171 (GRCm39)	missense	probably benign	0.44
R9287:Fat4	UTSW	3	38,945,781 (GRCm39)	missense	probably damaging	0.98
R9355:Fat4	UTSW	3	39,036,047 (GRCm39)	missense	probably damaging	1.00
R9437:Fat4	UTSW	3	38,945,417 (GRCm39)	missense	probably benign	0.00
R9456:Fat4	UTSW	3	38,942,571 (GRCm39)	missense	possibly damaging	0.50
R9476:Fat4	UTSW	3	39,037,886 (GRCm39)	missense	probably benign	0.04
R9510:Fat4	UTSW	3	39,037,886 (GRCm39)	missense	probably benign	0.04
R9511:Fat4	UTSW	3	39,034,802 (GRCm39)	missense	probably damaging	0.98
R9540:Fat4	UTSW	3	39,063,346 (GRCm39)	missense	probably benign
R9568:Fat4	UTSW	3	38,946,156 (GRCm39)	missense	probably damaging	1.00
R9646:Fat4	UTSW	3	39,035,813 (GRCm39)	missense	probably damaging	1.00
R9683:Fat4	UTSW	3	38,943,332 (GRCm39)	missense	possibly damaging	0.52
R9711:Fat4	UTSW	3	39,055,374 (GRCm39)	missense	probably benign	0.00
X0017:Fat4	UTSW	3	39,063,255 (GRCm39)	missense	probably benign	0.00
X0019:Fat4	UTSW	3	39,035,189 (GRCm39)	missense	probably damaging	1.00
X0020:Fat4	UTSW	3	39,054,300 (GRCm39)	missense	probably damaging	1.00
X0024:Fat4	UTSW	3	38,997,051 (GRCm39)	missense	probably benign	0.43
X0064:Fat4	UTSW	3	39,024,901 (GRCm39)	missense	probably damaging	1.00
Z1088:Fat4	UTSW	3	39,012,641 (GRCm39)	missense	probably benign	0.00
Z1088:Fat4	UTSW	3	38,941,199 (GRCm39)	missense	possibly damaging	0.88
Z1176:Fat4	UTSW	3	39,037,964 (GRCm39)	missense	probably damaging	1.00
Z1176:Fat4	UTSW	3	39,037,508 (GRCm39)	missense	probably benign	0.00
Z1177:Fat4	UTSW	3	38,944,496 (GRCm39)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,942,733 (GRCm39)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	39,035,987 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CACTGGGAGTATCTTTCTGGC -3'
(R):5'- GCTTGGTCATTGGCTTTCAC -3'

Sequencing Primer
(F):5'- CTGGGAGTATCTTTCTGGCCAAAAAG -3'
(R):5'- GGCTTTCACCGTTAACTCGAAG -3'

Posted On

2022-06-15