|Institutional Source||Beutler Lab|
|Gene Name||proteasome (prosome, macropain) 26S subunit, non-ATPase, 5|
|Essential gene?||Non essential (E-score: 0.000)|
|Stock #||R9457 (G1)|
|Chromosomal Location||34849734-34874968 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 34854326 bp (GRCm38)|
|Amino Acid Change||Serine to Proline at position 395 (S395P)|
|Ref Sequence||ENSEMBL: ENSMUSP00000028225 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028225]|
AA Change: S395P
PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
AA Change: S395P
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Psmd5||
(F):5'- AGGGTAGAAACTACTGCGTGC -3'
(R):5'- GTTCATCCCGTCCAACAAAG -3'
(F):5'- CACAGAGGCTAGCTCACTCTAGTG -3'
(R):5'- TCCAACAAAGATGGGAGCTTTCTG -3'