Mutagenetix > Incidental Mutation

Incidental Mutation 'R9457:Mllt6'

714667

Institutional Source

Beutler Lab

Gene Symbol

Mllt6

Ensembl Gene

ENSMUSG00000038437

Gene Name

myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6

Synonyms

Af17

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9457 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

97663414-97685463 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 97665760 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 92 (I92T)

Ref Sequence

ENSEMBL: ENSMUSP00000045445 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044730] [ENSMUST00000107586]

AlphaFold

B1AR10

Predicted Effect

probably benign
Transcript: ENSMUST00000044730
AA Change: I92T

PolyPhen 2 Score 0.124 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000045445
Gene: ENSMUSG00000038437
AA Change: I92T

Domain	Start	End	E-Value	Type
PHD	7	55	5.92e-7	SMART
PHD	119	178	5e-5	SMART
low complexity region	190	224	N/A	INTRINSIC
low complexity region	271	284	N/A	INTRINSIC
low complexity region	290	330	N/A	INTRINSIC
low complexity region	358	380	N/A	INTRINSIC
low complexity region	398	412	N/A	INTRINSIC
low complexity region	487	498	N/A	INTRINSIC
low complexity region	502	516	N/A	INTRINSIC
low complexity region	520	536	N/A	INTRINSIC
low complexity region	559	573	N/A	INTRINSIC
low complexity region	579	601	N/A	INTRINSIC
low complexity region	649	666	N/A	INTRINSIC
coiled coil region	703	744	N/A	INTRINSIC
low complexity region	746	756	N/A	INTRINSIC
low complexity region	773	782	N/A	INTRINSIC
low complexity region	802	848	N/A	INTRINSIC
low complexity region	860	901	N/A	INTRINSIC
coiled coil region	915	942	N/A	INTRINSIC
low complexity region	995	1018	N/A	INTRINSIC
low complexity region	1026	1037	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107586
AA Change: I92T

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000103212
Gene: ENSMUSG00000038437
AA Change: I92T

Domain	Start	End	E-Value	Type
PHD	7	55	5.92e-7	SMART
PHD	119	178	5e-5	SMART
low complexity region	190	224	N/A	INTRINSIC
low complexity region	271	284	N/A	INTRINSIC
low complexity region	290	330	N/A	INTRINSIC
low complexity region	336	356	N/A	INTRINSIC
low complexity region	388	410	N/A	INTRINSIC
low complexity region	428	442	N/A	INTRINSIC
low complexity region	517	528	N/A	INTRINSIC
low complexity region	532	546	N/A	INTRINSIC
low complexity region	550	566	N/A	INTRINSIC
low complexity region	589	603	N/A	INTRINSIC
low complexity region	609	631	N/A	INTRINSIC
low complexity region	679	696	N/A	INTRINSIC
coiled coil region	733	774	N/A	INTRINSIC
low complexity region	776	786	N/A	INTRINSIC
low complexity region	803	812	N/A	INTRINSIC
low complexity region	832	878	N/A	INTRINSIC
low complexity region	890	931	N/A	INTRINSIC
coiled coil region	945	972	N/A	INTRINSIC
low complexity region	1025	1048	N/A	INTRINSIC
low complexity region	1056	1067	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a gene trap allele exhibit no abnormal phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110002H16Rik	A	G	18: 12,179,246 (GRCm38)	H181R	probably benign	Het
4930404N11Rik	C	A	10: 81,365,777 (GRCm38)	V4L	probably benign	Het
4930430A15Rik	A	T	2: 111,170,286 (GRCm38)	M196K	unknown	Het
4932415D10Rik	A	G	10: 82,286,739 (GRCm38)	V3479A	probably benign	Het
A930009A15Rik	T	C	10: 115,578,331 (GRCm38)	L54P	unknown	Het
Ablim3	A	G	18: 61,845,849 (GRCm38)	S204P	probably benign	Het
Acadl	A	G	1: 66,853,241 (GRCm38)	V141A	probably benign	Het
Ace3	A	G	11: 105,994,861 (GRCm38)	D31G	probably benign	Het
Adam11	T	G	11: 102,769,898 (GRCm38)	V85G	probably benign	Het
Adarb1	A	T	10: 77,322,148 (GRCm38)	M155K	possibly damaging	Het
Arhgap10	G	A	8: 77,384,786 (GRCm38)	T376M	probably benign	Het
Bod1l	G	T	5: 41,821,967 (GRCm38)	T668K	probably damaging	Het
Ccdc87	A	G	19: 4,841,631 (GRCm38)	E717G	probably damaging	Het
Cdh17	T	A	4: 11,771,329 (GRCm38)	I37N	probably damaging	Het
Cfap99	T	G	5: 34,301,397 (GRCm38)	F45L	probably benign	Het
Chsy1	C	A	7: 66,172,400 (GRCm38)	H794Q	probably benign	Het
Clec4a3	G	A	6: 122,954,086 (GRCm38)	V45I	probably benign	Het
Clic3	G	A	2: 25,457,718 (GRCm38)	V32I	probably benign	Het
Clip2	A	T	5: 134,502,730 (GRCm38)	D740E	probably benign	Het
Col5a2	C	T	1: 45,386,844 (GRCm38)	V1062I	probably benign	Het
Col5a2	A	G	1: 45,392,813 (GRCm38)		probably null	Het
Cyp2j11	C	T	4: 96,307,359 (GRCm38)	V367I	probably damaging	Het
Ddr1	C	T	17: 35,682,758 (GRCm38)	A821T	possibly damaging	Het
Dna2	G	A	10: 62,950,793 (GRCm38)	E107K	probably benign	Het
Eif3d	A	G	15: 77,959,694 (GRCm38)	V484A	probably benign	Het
Fgfr4	A	G	13: 55,161,127 (GRCm38)	T354A	probably benign	Het
Fkbp6	T	C	5: 135,349,632 (GRCm38)	D54G	probably benign	Het
Gm4787	T	A	12: 81,379,246 (GRCm38)	E46V	probably damaging	Het
Gm47995	A	G	1: 151,198,475 (GRCm38)	T10A	possibly damaging	Het
Gm498	T	C	7: 143,883,976 (GRCm38)	V137A	possibly damaging	Het
Gnb1l	T	A	16: 18,540,995 (GRCm38)	I50N	probably damaging	Het
Gphb5	A	T	12: 75,415,749 (GRCm38)	V22D	probably damaging	Het
Gstt4	C	T	10: 75,815,125 (GRCm38)	C221Y	probably benign	Het
Hmces	T	C	6: 87,933,274 (GRCm38)	V222A	possibly damaging	Het
Kat14	T	A	2: 144,373,782 (GRCm38)	D62E	probably benign	Het
Kcnmb2	T	A	3: 32,181,869 (GRCm38)	V89E	probably benign	Het
Kif23	T	A	9: 61,944,225 (GRCm38)	N63I	probably benign	Het
Lamc2	T	C	1: 153,139,854 (GRCm38)	M578V	probably benign	Het
Ltbp2	A	T	12: 84,789,153 (GRCm38)	C1335S	probably benign	Het
Lyst	A	G	13: 13,687,745 (GRCm38)	E2622G	possibly damaging	Het
Mctp1	A	T	13: 76,384,674 (GRCm38)	H47L	probably benign	Het
Micalcl	A	G	7: 112,411,458 (GRCm38)	K618R	probably damaging	Het
Morc2a	A	G	11: 3,676,184 (GRCm38)	I223V	probably benign	Het
Msh3	A	T	13: 92,345,086 (GRCm38)	I306N	probably benign	Het
Myo3b	T	A	2: 70,095,209 (GRCm38)	S35T	probably benign	Het
Nfkbiz	A	T	16: 55,813,984 (GRCm38)	V700E	probably damaging	Het
Oit3	T	A	10: 59,441,683 (GRCm38)	M1L	unknown	Het
Olfr1048	A	T	2: 86,236,472 (GRCm38)	I114K	probably damaging	Het
Olfr1231	A	T	2: 89,302,731 (GRCm38)	I287N	probably damaging	Het
Olfr606	T	A	7: 103,451,411 (GRCm38)	F25I	probably benign	Het
Peg3	T	G	7: 6,707,999 (GRCm38)	D1408A	probably damaging	Het
Plaa	A	C	4: 94,586,883 (GRCm38)	S201R	possibly damaging	Het
Psmd5	A	G	2: 34,854,326 (GRCm38)	S395P	probably benign	Het
Ralgapa2	T	C	2: 146,334,554 (GRCm38)	I1701V	probably damaging	Het
Rnf32	G	A	5: 29,206,186 (GRCm38)	A157T	probably damaging	Het
Rrad	T	A	8: 104,629,727 (GRCm38)		probably null	Het
Samm50	T	A	15: 84,207,841 (GRCm38)	L339Q	probably damaging	Het
Scin	T	C	12: 40,104,958 (GRCm38)	E212G	possibly damaging	Het
Scrib	T	C	15: 76,067,299 (GRCm38)	D146G	probably damaging	Het
Slc19a1	G	A	10: 77,049,771 (GRCm38)	D502N	probably benign	Het
Slc4a4	T	C	5: 89,214,573 (GRCm38)	S839P	probably damaging	Het
Slc4a8	A	G	15: 100,806,260 (GRCm38)	D764G	probably damaging	Het
Slc6a19	G	A	13: 73,681,765 (GRCm38)	A590V	probably damaging	Het
Slfn8	G	T	11: 83,017,706 (GRCm38)	H4N	probably benign	Het
Smad9	T	A	3: 54,789,335 (GRCm38)	F274I	possibly damaging	Het
Snx4	A	G	16: 33,286,010 (GRCm38)	E271G	probably benign	Het
Thap4	G	A	1: 93,750,306 (GRCm38)	R253*	probably null	Het
Tmem65	T	A	15: 58,790,179 (GRCm38)	I144F		Het
Tnrc6a	G	A	7: 123,179,735 (GRCm38)	R1223Q	probably benign	Het
Traf7	CA	CAA	17: 24,527,763 (GRCm38)		probably benign	Het
Trpm2	T	C	10: 77,911,392 (GRCm38)	Y1424C	possibly damaging	Het
Trrap	T	A	5: 144,826,668 (GRCm38)	Y2457N	probably damaging	Het
Vmn1r7	A	G	6: 57,024,523 (GRCm38)	S251P	probably damaging	Het
Vmn2r89	A	T	14: 51,456,012 (GRCm38)	E273V	probably damaging	Het
Vps52	A	G	17: 33,962,182 (GRCm38)	D466G	probably damaging	Het
Xirp2	T	C	2: 67,515,632 (GRCm38)	V2739A	probably benign	Het
Zc3h4	C	T	7: 16,434,750 (GRCm38)	S1003F	unknown	Het
Zyg11a	A	T	4: 108,217,905 (GRCm38)	H6Q	probably damaging	Het

Other mutations in Mllt6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01745:Mllt6	APN	11	97,676,928 (GRCm38)	missense	probably damaging	1.00
IGL01808:Mllt6	APN	11	97,672,484 (GRCm38)	missense	possibly damaging	0.88
IGL01967:Mllt6	APN	11	97,674,777 (GRCm38)	missense	probably damaging	0.98
IGL02247:Mllt6	APN	11	97,670,332 (GRCm38)	missense	probably benign	0.01
IGL03161:Mllt6	APN	11	97,667,151 (GRCm38)	missense	probably benign	0.03
R0284:Mllt6	UTSW	11	97,678,605 (GRCm38)	missense	probably benign	0.02
R0718:Mllt6	UTSW	11	97,676,359 (GRCm38)	splice site	probably benign
R0783:Mllt6	UTSW	11	97,665,745 (GRCm38)	missense	probably damaging	0.99
R0811:Mllt6	UTSW	11	97,678,561 (GRCm38)	missense	probably damaging	0.97
R0812:Mllt6	UTSW	11	97,678,561 (GRCm38)	missense	probably damaging	0.97
R0904:Mllt6	UTSW	11	97,664,998 (GRCm38)	missense	probably damaging	1.00
R0960:Mllt6	UTSW	11	97,664,946 (GRCm38)	splice site	probably benign
R1445:Mllt6	UTSW	11	97,672,451 (GRCm38)	splice site	probably benign
R1523:Mllt6	UTSW	11	97,665,023 (GRCm38)	missense	probably damaging	1.00
R1781:Mllt6	UTSW	11	97,672,569 (GRCm38)	missense	probably benign
R1952:Mllt6	UTSW	11	97,677,222 (GRCm38)	missense	probably damaging	0.99
R2258:Mllt6	UTSW	11	97,664,976 (GRCm38)	missense	probably damaging	1.00
R2259:Mllt6	UTSW	11	97,664,976 (GRCm38)	missense	probably damaging	1.00
R2927:Mllt6	UTSW	11	97,680,776 (GRCm38)	missense	probably damaging	0.98
R4866:Mllt6	UTSW	11	97,674,459 (GRCm38)	missense	probably damaging	1.00
R4938:Mllt6	UTSW	11	97,678,407 (GRCm38)	missense	probably benign
R5039:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5058:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5064:Mllt6	UTSW	11	97,673,949 (GRCm38)	missense	probably damaging	1.00
R5076:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5115:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5379:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5509:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5510:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5511:Mllt6	UTSW	11	97,669,500 (GRCm38)	missense	possibly damaging	0.83
R5603:Mllt6	UTSW	11	97,673,505 (GRCm38)	missense	probably damaging	1.00
R5816:Mllt6	UTSW	11	97,672,574 (GRCm38)	missense	probably damaging	0.97
R6030:Mllt6	UTSW	11	97,677,225 (GRCm38)	missense	probably damaging	0.99
R6030:Mllt6	UTSW	11	97,677,225 (GRCm38)	missense	probably damaging	0.99
R6051:Mllt6	UTSW	11	97,680,743 (GRCm38)	nonsense	probably null
R6180:Mllt6	UTSW	11	97,678,536 (GRCm38)	missense	possibly damaging	0.85
R6277:Mllt6	UTSW	11	97,673,948 (GRCm38)	missense	probably damaging	1.00
R6667:Mllt6	UTSW	11	97,676,934 (GRCm38)	missense	probably damaging	1.00
R6754:Mllt6	UTSW	11	97,674,447 (GRCm38)	missense	probably damaging	1.00
R6791:Mllt6	UTSW	11	97,680,602 (GRCm38)	missense	probably damaging	0.98
R7049:Mllt6	UTSW	11	97,673,811 (GRCm38)	missense	probably damaging	1.00
R7194:Mllt6	UTSW	11	97,673,568 (GRCm38)	missense	probably benign	0.23
R7387:Mllt6	UTSW	11	97,674,600 (GRCm38)	missense	probably benign	0.04
R7484:Mllt6	UTSW	11	97,672,616 (GRCm38)	missense	probably benign	0.18
R7685:Mllt6	UTSW	11	97,676,964 (GRCm38)	missense	probably damaging	0.99
R7853:Mllt6	UTSW	11	97,670,316 (GRCm38)	missense	probably benign
R7862:Mllt6	UTSW	11	97,665,805 (GRCm38)	missense	probably benign	0.03
R8004:Mllt6	UTSW	11	97,676,140 (GRCm38)	missense	possibly damaging	0.91
R8498:Mllt6	UTSW	11	97,676,862 (GRCm38)	missense	possibly damaging	0.86
R9044:Mllt6	UTSW	11	97,663,659 (GRCm38)	missense	probably damaging	1.00
R9297:Mllt6	UTSW	11	97,672,488 (GRCm38)	missense	probably damaging	0.96
R9557:Mllt6	UTSW	11	97,673,484 (GRCm38)	missense	probably benign	0.04
Z1177:Mllt6	UTSW	11	97,676,425 (GRCm38)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- CGCACAAAGATGGGGCATTG -3'
(R):5'- ACCTATGAGCAACCCTCTGG -3'

Sequencing Primer
(F):5'- TGAACGCCTGCTGAGGTCAG -3'
(R):5'- TATGAGCAACCCTCTGGCTGTG -3'

Posted On

2022-06-15