Mutagenetix > Incidental Mutation

Incidental Mutation 'R9460:Lpar5'

714814

Institutional Source

Beutler Lab

Gene Symbol

Lpar5

Ensembl Gene

ENSMUSG00000067714

Gene Name

lysophosphatidic acid receptor 5

Synonyms

Gpr92, LOC381810, GPR93, LPA5

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R9460 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

125044883-125059435 bp(+) (GRCm39)

Type of Mutation

start gained

DNA Base Change (assembly)

A to G at 125058234 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000132511 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088292] [ENSMUST00000140346] [ENSMUST00000171989]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000088292

SMART Domains

Protein: ENSMUSP00000085630
Gene: ENSMUSG00000067714

Domain	Start	End	E-Value	Type
low complexity region	15	27	N/A	INTRINSIC
Pfam:7tm_1	55	313	7.4e-40	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000140346

SMART Domains

Protein: ENSMUSP00000119904
Gene: ENSMUSG00000067714

Domain	Start	End	E-Value	Type
low complexity region	15	27	N/A	INTRINSIC
Pfam:7tm_1	55	164	1.5e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171989

SMART Domains

Protein: ENSMUSP00000132511
Gene: ENSMUSG00000067714

Domain	Start	End	E-Value	Type
low complexity region	15	27	N/A	INTRINSIC
Pfam:7tm_1	55	313	1.1e-48	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (56/56)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the rhodopsin class of G protein-coupled transmembrane receptors. This protein transmits extracellular signals from lysophosphatidic acid to cells through heterotrimeric G proteins and mediates numerous cellular processes. Many G protein receptors serve as targets for pharmaceutical drugs. Transcript variants of this gene have been described.[provided by RefSeq, Dec 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to neuropathic pain and myelin sheath alterations. Mice homozygous for a different targeted allele exhibit decreased nociception sensitivity, decreased anxiety-related response and enhanced coordination and spatial learning. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsbg3	C	T	17: 57,183,316 (GRCm39)	T19I	probably damaging	Het
Alg1	T	A	16: 5,060,425 (GRCm39)	M382K	probably damaging	Het
Arhgap17	A	G	7: 122,879,286 (GRCm39)	S822P	unknown	Het
Bend7	A	C	2: 4,749,302 (GRCm39)	T140P	probably benign	Het
C1rb	T	G	6: 124,557,865 (GRCm39)	D667E	probably benign	Het
Carmil1	A	G	13: 24,253,750 (GRCm39)	C794R	probably damaging	Het
Cd36	T	C	5: 18,000,608 (GRCm39)	D365G	probably null	Het
Cep164	T	C	9: 45,685,282 (GRCm39)	N825S	probably benign	Het
Cp	T	C	3: 20,018,566 (GRCm39)	L90P		Het
Cpeb2	C	T	5: 43,390,769 (GRCm39)		probably benign	Het
Cramp1	A	G	17: 25,222,281 (GRCm39)	S146P	probably damaging	Het
Csmd3	A	T	15: 47,617,130 (GRCm39)	D1019E		Het
Cyp2d10	T	A	15: 82,289,470 (GRCm39)	D214V	probably benign	Het
Dcaf17	G	C	2: 70,917,695 (GRCm39)	V406L	possibly damaging	Het
Ehmt1	A	G	2: 24,728,791 (GRCm39)	V703A	probably benign	Het
Ermp1	A	C	19: 29,609,916 (GRCm39)	V293G	probably benign	Het
Fancd2os	C	A	6: 113,574,569 (GRCm39)	V146F	probably benign	Het
Galnt12	C	T	4: 47,117,983 (GRCm39)	T426I	probably damaging	Het
Gm11011	T	A	2: 169,429,289 (GRCm39)	T44S	unknown	Het
Gm2832	C	T	14: 41,000,843 (GRCm39)	T27I		Het
Klhl32	A	G	4: 24,649,866 (GRCm39)	V310A	probably benign	Het
Kntc1	T	A	5: 123,941,378 (GRCm39)	C1728*	probably null	Het
Lama2	T	A	10: 27,298,475 (GRCm39)	N207I	probably damaging	Het
Map3k8	T	C	18: 4,349,277 (GRCm39)	I14V	probably benign	Het
Marf1	A	T	16: 13,947,526 (GRCm39)	V1151E	probably damaging	Het
Me1	G	A	9: 86,495,685 (GRCm39)	A274V	probably damaging	Het
Mybpc1	A	T	10: 88,372,197 (GRCm39)	I797N	probably damaging	Het
Myo15a	T	A	11: 60,372,566 (GRCm39)		probably null	Het
N4bp2	A	T	5: 65,963,886 (GRCm39)	D645V	probably benign	Het
Nlrp4f	G	T	13: 65,342,006 (GRCm39)	D546E	possibly damaging	Het
Npc1	T	C	18: 12,346,398 (GRCm39)	D266G	possibly damaging	Het
Or4a81	G	A	2: 89,618,778 (GRCm39)	S306L	probably benign	Het
Or8k16	A	G	2: 85,520,359 (GRCm39)	I195M	probably benign	Het
Pcdha12	C	T	18: 37,153,574 (GRCm39)	R98W	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Pigr	T	C	1: 130,772,403 (GRCm39)	I207T	probably damaging	Het
Plxnc1	T	A	10: 94,700,895 (GRCm39)	E596D	probably benign	Het
Polr1e	C	T	4: 45,018,691 (GRCm39)	P7L	probably benign	Het
Ppp5c	G	T	7: 16,741,137 (GRCm39)	Y313*	probably null	Het
Sacs	C	A	14: 61,441,611 (GRCm39)	T1219K	probably benign	Het
Scn3a	A	G	2: 65,300,535 (GRCm39)	V1277A	probably damaging	Het
Serinc5	G	T	13: 92,844,607 (GRCm39)	A450S	possibly damaging	Het
Serinc5	T	C	13: 92,844,619 (GRCm39)	C454R	probably benign	Het
Slc12a6	G	A	2: 112,183,280 (GRCm39)	V771I	probably benign	Het
Slc4a1ap	T	C	5: 31,685,463 (GRCm39)	I247T	probably benign	Het
Snx10	T	A	6: 51,565,888 (GRCm39)	S184R	probably damaging	Het
Tgm2	A	T	2: 157,971,241 (GRCm39)		probably null	Het
Thap1	CAGCATCTGCTCGGAGCA	CAGCA	8: 26,650,884 (GRCm39)		probably null	Het
Thsd7b	T	C	1: 130,090,674 (GRCm39)		probably null	Het
Tiam2	G	A	17: 3,487,585 (GRCm39)	G702E	probably damaging	Het
Tmco4	T	A	4: 138,747,387 (GRCm39)	V212D	probably damaging	Het
Usp48	G	A	4: 137,340,996 (GRCm39)	G332E	probably benign	Het
Vipas39	A	G	12: 87,288,021 (GRCm39)	L482P	probably damaging	Het
Vmn1r5	T	A	6: 56,962,829 (GRCm39)	M168K		Het
Vps13c	T	C	9: 67,837,904 (GRCm39)	L1818S	possibly damaging	Het
Vwa2	A	G	19: 56,886,388 (GRCm39)	I152V	probably benign	Het
Zfp180	G	A	7: 23,804,399 (GRCm39)	G273R	probably damaging	Het
Zpld2	G	A	4: 133,929,312 (GRCm39)	P331L	probably benign	Het

Other mutations in Lpar5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01719:Lpar5	APN	6	125,058,969 (GRCm39)	missense	possibly damaging	0.94
IGL01830:Lpar5	APN	6	125,058,785 (GRCm39)	missense	probably benign	0.01
IGL01975:Lpar5	APN	6	125,058,750 (GRCm39)	missense	probably damaging	0.99
IGL02021:Lpar5	APN	6	125,058,955 (GRCm39)	nonsense	probably null
IGL02718:Lpar5	APN	6	125,059,207 (GRCm39)	missense	probably damaging	1.00
IGL03027:Lpar5	APN	6	125,059,203 (GRCm39)	missense	probably damaging	1.00
IGL03300:Lpar5	APN	6	125,059,203 (GRCm39)	missense	probably damaging	1.00
F5770:Lpar5	UTSW	6	125,058,690 (GRCm39)	missense	possibly damaging	0.88
R0633:Lpar5	UTSW	6	125,058,954 (GRCm39)	missense	probably benign	0.25
R1639:Lpar5	UTSW	6	125,058,564 (GRCm39)	missense	probably damaging	1.00
R1822:Lpar5	UTSW	6	125,058,378 (GRCm39)	missense	possibly damaging	0.76
R2227:Lpar5	UTSW	6	125,058,098 (GRCm39)	critical splice acceptor site	probably null
R4019:Lpar5	UTSW	6	125,058,638 (GRCm39)	missense	probably damaging	1.00
R4288:Lpar5	UTSW	6	125,058,827 (GRCm39)	missense	probably benign	0.00
R4705:Lpar5	UTSW	6	125,059,170 (GRCm39)	missense	possibly damaging	0.64
R4787:Lpar5	UTSW	6	125,059,461 (GRCm39)	splice site	probably null
R5027:Lpar5	UTSW	6	125,059,110 (GRCm39)	missense	possibly damaging	0.69
R6114:Lpar5	UTSW	6	125,058,639 (GRCm39)	missense	probably damaging	1.00
R7197:Lpar5	UTSW	6	125,059,347 (GRCm39)	missense	probably benign	0.00
R7779:Lpar5	UTSW	6	125,059,207 (GRCm39)	missense	probably damaging	1.00
R8193:Lpar5	UTSW	6	125,058,302 (GRCm39)	missense	probably benign
R8264:Lpar5	UTSW	6	125,058,465 (GRCm39)	missense	probably damaging	1.00
R9628:Lpar5	UTSW	6	125,058,948 (GRCm39)	missense	probably damaging	0.96
V7580:Lpar5	UTSW	6	125,058,690 (GRCm39)	missense	possibly damaging	0.88
V7581:Lpar5	UTSW	6	125,058,690 (GRCm39)	missense	possibly damaging	0.88
V7582:Lpar5	UTSW	6	125,058,690 (GRCm39)	missense	possibly damaging	0.88
Z1176:Lpar5	UTSW	6	125,059,035 (GRCm39)	missense	probably damaging	1.00
Z1176:Lpar5	UTSW	6	125,058,342 (GRCm39)	missense	possibly damaging	0.92
Z1177:Lpar5	UTSW	6	125,058,981 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCTTTGAGGCCAAGAAAGGAC -3'
(R):5'- CCACCATATGCAAACGATGTG -3'

Sequencing Primer
(F):5'- CAGCCAAGTGTTTAGGCTACG -3'
(R):5'- GTATCTCGATAGTCAGGGCAC -3'

Posted On

2022-06-15