Mutagenetix > Incidental Mutation

Incidental Mutation 'R9463:Or7g27'

715007

Institutional Source

Beutler Lab

Gene Symbol

Or7g27

Ensembl Gene

ENSMUSG00000061614

Gene Name

olfactory receptor family 7 subfamily G member 27

Synonyms

MOR150-1P, GA_x6K02T2PVTD-13076685-13077623, MOR150-2, Olfr1522-ps1, MOR150-1, Olfr845, MOR150-1P

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R9463 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19249755-19250696 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 19250320 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 188 (A188V)

Ref Sequence

ENSEMBL: ENSMUSP00000150474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071259] [ENSMUST00000213344] [ENSMUST00000215572]

AlphaFold

Q7TRG2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000071259
AA Change: A188V

PolyPhen 2 Score 0.938 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000071239
Gene: ENSMUSG00000061614
AA Change: A188V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.1e-56	PFAM
Pfam:7tm_1	41	290	2.1e-22	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213344
AA Change: A188V

PolyPhen 2 Score 0.938 (Sensitivity: 0.80; Specificity: 0.94)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215572
AA Change: A188V

PolyPhen 2 Score 0.938 (Sensitivity: 0.80; Specificity: 0.94)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox2	C	T	14: 8,256,789 (GRCm38)	E25K	probably damaging	Het
Acss2	A	G	2: 155,392,032 (GRCm39)	Y218C	probably benign	Het
Armc8	C	T	9: 99,378,203 (GRCm39)		probably null	Het
Aspg	G	A	12: 112,089,824 (GRCm39)	G454D	probably damaging	Het
Atp9b	T	C	18: 80,809,051 (GRCm39)	T623A		Het
Cacna1e	A	G	1: 154,357,411 (GRCm39)	L655P	probably damaging	Het
Cant1	T	A	11: 118,302,281 (GRCm39)	N12I	probably damaging	Het
Cep63	T	C	9: 102,475,382 (GRCm39)	T445A	probably benign	Het
Clstn1	A	T	4: 149,698,564 (GRCm39)	D50V	possibly damaging	Het
Cnnm2	T	A	19: 46,750,990 (GRCm39)	I260N	probably damaging	Het
Col4a4	T	C	1: 82,431,076 (GRCm39)	M1609V	unknown	Het
Dand5	A	T	8: 85,542,938 (GRCm39)	C179*	probably null	Het
Dbndd1	A	G	8: 124,233,547 (GRCm39)	L153P	probably damaging	Het
Dnhd1	A	G	7: 105,306,454 (GRCm39)	I437V	probably benign	Het
Dnhd1	T	A	7: 105,344,223 (GRCm39)	S1856T	probably benign	Het
Dpp8	T	A	9: 64,973,700 (GRCm39)	Y641*	probably null	Het
Dthd1	G	A	5: 63,039,626 (GRCm39)	R676H	probably benign	Het
Efl1	C	T	7: 82,426,733 (GRCm39)	T1077M	probably damaging	Het
Eif3e	G	T	15: 43,138,709 (GRCm39)	Q83K	probably benign	Het
Firrm	A	T	1: 163,795,907 (GRCm39)	M428K	probably benign	Het
Gfm2	G	A	13: 97,286,910 (GRCm39)	A170T	probably damaging	Het
Gm14496	T	A	2: 181,642,256 (GRCm39)	H642Q	probably benign	Het
Gmip	G	A	8: 70,269,693 (GRCm39)	R596Q	possibly damaging	Het
Hook1	A	T	4: 95,910,510 (GRCm39)	Q708L	probably damaging	Het
Krtap9-3	G	A	11: 99,488,526 (GRCm39)	R119C	unknown	Het
Lnpk	A	C	2: 74,381,403 (GRCm39)		probably null	Het
Lrp1	G	T	10: 127,429,334 (GRCm39)	Y484*	probably null	Het
Lrrc8e	C	T	8: 4,285,185 (GRCm39)	P470L	probably damaging	Het
Mapre1	A	G	2: 153,606,960 (GRCm39)	N231D	probably benign	Het
Muc5b	T	A	7: 141,405,503 (GRCm39)	N937K	unknown	Het
Ndufaf7	T	C	17: 79,253,900 (GRCm39)		probably null	Het
Nlrp5	A	T	7: 23,118,225 (GRCm39)	I650F	probably benign	Het
Notch1	T	C	2: 26,359,845 (GRCm39)	D1290G	probably benign	Het
Or8b46	T	C	9: 38,450,665 (GRCm39)	I158T	probably damaging	Het
Orc4	C	T	2: 48,826,783 (GRCm39)		probably null	Het
Plekhm2	A	G	4: 141,357,949 (GRCm39)	V664A	probably benign	Het
Pom121l2	T	G	13: 22,168,402 (GRCm39)	I891S	probably benign	Het
Prkcsh	T	C	9: 21,923,982 (GRCm39)	Y425H	probably damaging	Het
Prps1l1	A	C	12: 35,035,559 (GRCm39)	T225P	probably damaging	Het
Prr18	T	G	17: 8,560,324 (GRCm39)	V160G	probably damaging	Het
Psd2	T	A	18: 36,143,798 (GRCm39)	F701L	probably damaging	Het
Sclt1	T	C	3: 41,601,931 (GRCm39)	E593G	probably damaging	Het
Sdk1	T	C	5: 141,948,548 (GRCm39)	I631T	probably benign	Het
Sirt1	A	T	10: 63,171,487 (GRCm39)	D231E	probably benign	Het
Slc22a30	C	T	19: 8,378,259 (GRCm39)	C139Y	probably damaging	Het
Sntg1	T	A	1: 8,624,974 (GRCm39)	N274I	probably damaging	Het
Spocd1	A	G	4: 129,847,398 (GRCm39)	Q529R		Het
Trarg1	T	A	11: 76,571,098 (GRCm39)	L38Q	probably damaging	Het
Trbv3	T	A	6: 41,025,530 (GRCm39)	L40H	probably damaging	Het
Trpm6	T	C	19: 18,761,264 (GRCm39)		probably null	Het
Ttn	A	G	2: 76,578,519 (GRCm39)	Y24125H	probably damaging	Het
Ush1g	C	A	11: 115,209,056 (GRCm39)	L379F	probably damaging	Het
Vipr1	G	A	9: 121,471,993 (GRCm39)		probably null	Het
Vmn2r73	A	T	7: 85,506,795 (GRCm39)	M839K		Het
Wee1	T	C	7: 109,721,917 (GRCm39)	S121P	probably damaging	Het
Zfp608	T	C	18: 55,030,274 (GRCm39)	K1222R	probably damaging	Het

Other mutations in Or7g27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01546:Or7g27	APN	9	19,250,068 (GRCm39)	missense	possibly damaging	0.56
IGL01637:Or7g27	APN	9	19,250,260 (GRCm39)	missense	probably damaging	1.00
IGL01767:Or7g27	APN	9	19,250,598 (GRCm39)	missense	possibly damaging	0.54
IGL01945:Or7g27	APN	9	19,250,628 (GRCm39)	missense	probably damaging	0.98
IGL02202:Or7g27	APN	9	19,250,545 (GRCm39)	missense	probably benign	0.06
IGL02877:Or7g27	APN	9	19,250,497 (GRCm39)	missense	possibly damaging	0.86
R0466:Or7g27	UTSW	9	19,250,475 (GRCm39)	missense	probably damaging	1.00
R1521:Or7g27	UTSW	9	19,249,948 (GRCm39)	missense	probably benign	0.35
R1650:Or7g27	UTSW	9	19,249,943 (GRCm39)	missense	possibly damaging	0.49
R1766:Or7g27	UTSW	9	19,250,154 (GRCm39)	missense	probably benign	0.06
R2060:Or7g27	UTSW	9	19,250,352 (GRCm39)	missense	probably benign	0.01
R2082:Or7g27	UTSW	9	19,250,574 (GRCm39)	missense	probably benign	0.36
R2257:Or7g27	UTSW	9	19,249,789 (GRCm39)	missense	probably benign	0.01
R2892:Or7g27	UTSW	9	19,250,034 (GRCm39)	missense	probably benign	0.04
R3156:Or7g27	UTSW	9	19,250,720 (GRCm39)	splice site	probably null
R3943:Or7g27	UTSW	9	19,250,371 (GRCm39)	missense	probably benign	0.05
R4116:Or7g27	UTSW	9	19,249,940 (GRCm39)	missense	probably benign	0.39
R4518:Or7g27	UTSW	9	19,250,556 (GRCm39)	missense	possibly damaging	0.86
R4814:Or7g27	UTSW	9	19,250,476 (GRCm39)	missense	probably damaging	1.00
R5339:Or7g27	UTSW	9	19,250,455 (GRCm39)	missense	possibly damaging	0.78
R6647:Or7g27	UTSW	9	19,249,925 (GRCm39)	missense	possibly damaging	0.50
R7493:Or7g27	UTSW	9	19,250,109 (GRCm39)	missense	probably damaging	0.98
R7522:Or7g27	UTSW	9	19,250,294 (GRCm39)	nonsense	probably null
R7584:Or7g27	UTSW	9	19,250,569 (GRCm39)	missense	possibly damaging	0.94
R9127:Or7g27	UTSW	9	19,250,026 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TTGCAGCAATGGCCTATGAC -3'
(R):5'- AACTACTGACAGGTGAGAGCC -3'

Sequencing Primer
(F):5'- AATGGCCTATGACCGCTATG -3'
(R):5'- CACACGTGGAAAATGCTTTATGCC -3'

Posted On

2022-06-15