Mutagenetix > Incidental Mutation

Incidental Mutation 'R9464:Zfp956'

715055

Institutional Source

Beutler Lab

Gene Symbol

Zfp956

Ensembl Gene

ENSMUSG00000045466

Gene Name

zinc finger protein 956

Synonyms

AI894139

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R9464 (G1)

Quality Score

211.009

Status

Not validated

Chromosome

Chromosomal Location

47930324-47942234 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 47941041 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 467 (C467S)

Ref Sequence

ENSEMBL: ENSMUSP00000098989 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000101445] [ENSMUST00000140719]

AlphaFold

Q3UZY6

Predicted Effect

probably damaging
Transcript: ENSMUST00000101445
AA Change: C467S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000098989
Gene: ENSMUSG00000045466
AA Change: C467S

Domain	Start	End	E-Value	Type
Pfam:DUF3669	39	99	2.1e-9	PFAM
KRAB	129	191	2.4e-1	SMART
ZnF_C2H2	325	347	2.24e-3	SMART
ZnF_C2H2	353	375	2.09e-3	SMART
ZnF_C2H2	381	403	6.78e-3	SMART
ZnF_C2H2	409	431	2.57e-3	SMART
ZnF_C2H2	437	459	3.16e-3	SMART
ZnF_C2H2	465	487	3.58e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000140719

SMART Domains

Protein: ENSMUSP00000114227
Gene: ENSMUSG00000045466

Domain	Start	End	E-Value	Type
Pfam:DUF3669	22	101	2.4e-16	PFAM
Blast:KRAB	129	179	2e-20	BLAST

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930523C07Rik	T	C	1: 159,902,865 (GRCm39)	F45S	possibly damaging	Het
Abca4	A	G	3: 121,913,714 (GRCm39)	T897A	probably benign	Het
Abhd5	A	T	9: 122,208,029 (GRCm39)	T308S	probably benign	Het
Abi2	G	A	1: 60,478,100 (GRCm39)		probably null	Het
Abi3bp	C	A	16: 56,409,046 (GRCm39)	T283N	possibly damaging	Het
Adcy2	A	C	13: 68,882,776 (GRCm39)	N320K	probably damaging	Het
Adcy3	T	A	12: 4,256,939 (GRCm39)	M819K	probably benign	Het
Anapc5	A	G	5: 122,940,209 (GRCm39)	V354A	probably benign	Het
Card14	A	G	11: 119,208,031 (GRCm39)	I34V	probably benign	Het
Ccdc153	A	T	9: 44,157,011 (GRCm39)	T118S	possibly damaging	Het
Champ1	G	A	8: 13,929,114 (GRCm39)	G424D	probably damaging	Het
Crybg3	A	T	16: 59,376,120 (GRCm39)		probably benign	Het
Ctsr	T	A	13: 61,307,295 (GRCm39)	I334F	possibly damaging	Het
Dlgap1	A	T	17: 70,823,964 (GRCm39)	Q316H	probably benign	Het
Dnajc18	A	C	18: 35,830,166 (GRCm39)	C80G	probably damaging	Het
Dthd1	G	A	5: 63,039,626 (GRCm39)	R676H	probably benign	Het
Fam186b	G	A	15: 99,177,616 (GRCm39)	A570V	probably damaging	Het
Fancc	T	C	13: 63,550,769 (GRCm39)	K18E	possibly damaging	Het
Fbxo46	G	C	7: 18,870,791 (GRCm39)	R470P	probably damaging	Het
Fbxw20	A	C	9: 109,050,399 (GRCm39)	W409G	probably damaging	Het
Gcnt4	C	T	13: 97,083,493 (GRCm39)	T263M	probably benign	Het
Gfy	T	C	7: 44,827,251 (GRCm39)	T282A	probably benign	Het
Glb1l3	A	G	9: 26,761,351 (GRCm39)	M227T	probably damaging	Het
Gm8267	T	C	14: 44,960,346 (GRCm39)	E133G	probably damaging	Het
Hmcn1	A	C	1: 150,599,248 (GRCm39)	I1556R	possibly damaging	Het
Ifnl3	T	C	7: 28,223,287 (GRCm39)	V102A	probably damaging	Het
Kifc3	C	T	8: 95,830,622 (GRCm39)	R548Q	possibly damaging	Het
Lonrf1	T	A	8: 36,690,024 (GRCm39)	Q678L	probably benign	Het
Map2	A	G	1: 66,454,497 (GRCm39)	E1129G	probably damaging	Het
Mug2	C	A	6: 122,028,690 (GRCm39)	D561E	probably benign	Het
Myo3a	G	T	2: 22,232,383 (GRCm39)		probably benign	Het
N4bp1	A	G	8: 87,587,165 (GRCm39)	V591A	probably damaging	Het
Nckap5	C	T	1: 125,952,494 (GRCm39)	G1353R	probably benign	Het
Nek10	T	A	14: 14,937,766 (GRCm38)	H828Q	probably benign	Het
Or52n3	T	A	7: 104,530,050 (GRCm39)	C45*	probably null	Het
Or5k1b	G	T	16: 58,581,202 (GRCm39)	C112*	probably null	Het
Pclo	T	C	5: 14,816,830 (GRCm39)	S1297P		Het
Pkhd1l1	T	C	15: 44,343,009 (GRCm39)	I162T	probably damaging	Het
Plaat5	A	G	19: 7,591,923 (GRCm39)	E113G	probably damaging	Het
Plekhg2	T	C	7: 28,062,297 (GRCm39)	E541G	probably damaging	Het
Ppp1r15a	T	C	7: 45,174,149 (GRCm39)	N220D	possibly damaging	Het
Prss3l	A	G	6: 41,420,486 (GRCm39)	V80A	probably benign	Het
Psmd4	A	G	3: 94,940,735 (GRCm39)	S343P	probably benign	Het
Rnf213	A	T	11: 119,354,406 (GRCm39)	H4063L		Het
Rnf40	T	C	7: 127,190,954 (GRCm39)	V313A	probably benign	Het
Ryr2	T	A	13: 11,752,680 (GRCm39)	E1880D	probably benign	Het
Slc30a4	A	T	2: 122,527,200 (GRCm39)	L398I	probably damaging	Het
Sp100	A	T	1: 85,624,751 (GRCm39)	N380I	probably damaging	Het
Speer4d	T	G	5: 15,828,393 (GRCm39)	L175W	probably damaging	Het
Srcap	T	A	7: 127,137,273 (GRCm39)	I985K	possibly damaging	Het
Tesk2	T	C	4: 116,658,443 (GRCm39)		probably null	Het
Tgfbrap1	A	G	1: 43,114,608 (GRCm39)	V164A	probably damaging	Het
Tlk1	T	C	2: 70,544,341 (GRCm39)	T765A	probably benign	Het
Tmem207	A	T	16: 26,345,413 (GRCm39)	C18S		Het
Tph2	A	T	10: 114,915,992 (GRCm39)	C394S	probably benign	Het
Trrap	C	A	5: 144,763,517 (GRCm39)	H2470N	probably damaging	Het
Ttc21b	T	C	2: 66,053,866 (GRCm39)	Y771C	probably damaging	Het
Uchl3	T	C	14: 101,904,451 (GRCm39)	F122L	probably damaging	Het
Vipr1	G	A	9: 121,471,993 (GRCm39)		probably null	Het
Vmn2r117	G	T	17: 23,696,578 (GRCm39)	D276E	probably benign	Het
Vps13c	T	C	9: 67,858,674 (GRCm39)	L2708P	probably damaging	Het
Vwa3a	T	A	7: 120,385,682 (GRCm39)	S675T	possibly damaging	Het
Zfp473	A	G	7: 44,383,766 (GRCm39)	L189P	probably benign	Het
Zgrf1	T	A	3: 127,377,741 (GRCm39)	N995K	probably benign	Het

Other mutations in Zfp956

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01110:Zfp956	APN	6	47,940,346 (GRCm39)	missense	probably benign	0.28
ANU74:Zfp956	UTSW	6	47,940,507 (GRCm39)	missense	probably benign	0.00
R0603:Zfp956	UTSW	6	47,932,962 (GRCm39)	missense	probably damaging	1.00
R1243:Zfp956	UTSW	6	47,932,985 (GRCm39)	missense	probably damaging	0.98
R1879:Zfp956	UTSW	6	47,940,678 (GRCm39)	missense	probably benign	0.19
R1999:Zfp956	UTSW	6	47,940,805 (GRCm39)	missense	probably damaging	1.00
R2106:Zfp956	UTSW	6	47,941,359 (GRCm39)	makesense	probably null
R2150:Zfp956	UTSW	6	47,940,805 (GRCm39)	missense	probably damaging	1.00
R4737:Zfp956	UTSW	6	47,939,476 (GRCm39)	missense	probably damaging	0.99
R4740:Zfp956	UTSW	6	47,939,476 (GRCm39)	missense	probably damaging	0.99
R4751:Zfp956	UTSW	6	47,940,510 (GRCm39)	missense	probably benign	0.00
R4832:Zfp956	UTSW	6	47,928,987 (GRCm39)	unclassified	probably benign
R5325:Zfp956	UTSW	6	47,928,012 (GRCm39)	unclassified	probably benign
R5523:Zfp956	UTSW	6	47,930,455 (GRCm39)	start gained	probably benign
R6842:Zfp956	UTSW	6	47,940,763 (GRCm39)	missense	possibly damaging	0.47
R7131:Zfp956	UTSW	6	47,932,781 (GRCm39)	missense	probably benign	0.00
R7367:Zfp956	UTSW	6	47,940,853 (GRCm39)	missense	probably damaging	1.00
R8806:Zfp956	UTSW	6	47,933,042 (GRCm39)	missense	probably benign	0.00
R9074:Zfp956	UTSW	6	47,939,462 (GRCm39)	missense	possibly damaging	0.67
R9248:Zfp956	UTSW	6	47,934,437 (GRCm39)	missense	possibly damaging	0.92
R9452:Zfp956	UTSW	6	47,940,370 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CGCCTGTCTACTCTAGTGGAAC -3'
(R):5'- GAGGGTCACTGGAGAACTTTCC -3'

Sequencing Primer
(F):5'- TCTACTCTAGTGGAACATAGGCGC -3'
(R):5'- ACTGGAGAACTTTCCCCTGG -3'

Posted On

2022-06-15