Incidental Mutation 'R9464:Nek10'
| ID |
715086 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nek10
|
| Ensembl Gene |
ENSMUSG00000042567 |
| Gene Name |
NIMA (never in mitosis gene a)- related kinase 10 |
| Synonyms |
LOC238944 |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R9464 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
14 |
| Chromosomal Location |
7457704-7666183 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 14937766 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Histidine to Glutamine
at position 828
(H828Q)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000108249
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000112630]
[ENSMUST00000112631]
[ENSMUST00000224491]
|
| AlphaFold |
Q3UGM2 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112630
AA Change: H828Q
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000108249
Gene: ENSMUSG00000042567
AA Change: H828Q
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112631
AA Change: H828Q
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000108250
Gene: ENSMUSG00000042567
AA Change: H828Q
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000136826
AA Change: H131Q
PolyPhen 2
Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
|
| SMART Domains |
Protein: ENSMUSP00000123151
Gene: ENSMUSG00000042567
AA Change: H131Q
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Pkinase
|
1 |
87 |
5.1e-13 |
PFAM |
|
Pfam:Pkinase_Tyr
|
1 |
87 |
4.2e-8 |
PFAM |
|
low complexity region
|
103 |
115 |
N/A |
INTRINSIC |
|
low complexity region
|
143 |
167 |
N/A |
INTRINSIC |
|
low complexity region
|
212 |
230 |
N/A |
INTRINSIC |
|
low complexity region
|
257 |
268 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000224491
AA Change: H828Q
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.4%
- 20x: 97.9%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 64 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930523C07Rik |
T |
C |
1: 159,902,865 (GRCm39) |
F45S |
possibly damaging |
Het |
| Abca4 |
A |
G |
3: 121,913,714 (GRCm39) |
T897A |
probably benign |
Het |
| Abhd5 |
A |
T |
9: 122,208,029 (GRCm39) |
T308S |
probably benign |
Het |
| Abi2 |
G |
A |
1: 60,478,100 (GRCm39) |
|
probably null |
Het |
| Abi3bp |
C |
A |
16: 56,409,046 (GRCm39) |
T283N |
possibly damaging |
Het |
| Adcy2 |
A |
C |
13: 68,882,776 (GRCm39) |
N320K |
probably damaging |
Het |
| Adcy3 |
T |
A |
12: 4,256,939 (GRCm39) |
M819K |
probably benign |
Het |
| Anapc5 |
A |
G |
5: 122,940,209 (GRCm39) |
V354A |
probably benign |
Het |
| Card14 |
A |
G |
11: 119,208,031 (GRCm39) |
I34V |
probably benign |
Het |
| Ccdc153 |
A |
T |
9: 44,157,011 (GRCm39) |
T118S |
possibly damaging |
Het |
| Champ1 |
G |
A |
8: 13,929,114 (GRCm39) |
G424D |
probably damaging |
Het |
| Crybg3 |
A |
T |
16: 59,376,120 (GRCm39) |
|
probably benign |
Het |
| Ctsr |
T |
A |
13: 61,307,295 (GRCm39) |
I334F |
possibly damaging |
Het |
| Dlgap1 |
A |
T |
17: 70,823,964 (GRCm39) |
Q316H |
probably benign |
Het |
| Dnajc18 |
A |
C |
18: 35,830,166 (GRCm39) |
C80G |
probably damaging |
Het |
| Dthd1 |
G |
A |
5: 63,039,626 (GRCm39) |
R676H |
probably benign |
Het |
| Fam186b |
G |
A |
15: 99,177,616 (GRCm39) |
A570V |
probably damaging |
Het |
| Fancc |
T |
C |
13: 63,550,769 (GRCm39) |
K18E |
possibly damaging |
Het |
| Fbxo46 |
G |
C |
7: 18,870,791 (GRCm39) |
R470P |
probably damaging |
Het |
| Fbxw20 |
A |
C |
9: 109,050,399 (GRCm39) |
W409G |
probably damaging |
Het |
| Gcnt4 |
C |
T |
13: 97,083,493 (GRCm39) |
T263M |
probably benign |
Het |
| Gfy |
T |
C |
7: 44,827,251 (GRCm39) |
T282A |
probably benign |
Het |
| Glb1l3 |
A |
G |
9: 26,761,351 (GRCm39) |
M227T |
probably damaging |
Het |
| Gm8267 |
T |
C |
14: 44,960,346 (GRCm39) |
E133G |
probably damaging |
Het |
| Hmcn1 |
A |
C |
1: 150,599,248 (GRCm39) |
I1556R |
possibly damaging |
Het |
| Ifnl3 |
T |
C |
7: 28,223,287 (GRCm39) |
V102A |
probably damaging |
Het |
| Kifc3 |
C |
T |
8: 95,830,622 (GRCm39) |
R548Q |
possibly damaging |
Het |
| Lonrf1 |
T |
A |
8: 36,690,024 (GRCm39) |
Q678L |
probably benign |
Het |
| Map2 |
A |
G |
1: 66,454,497 (GRCm39) |
E1129G |
probably damaging |
Het |
| Mug2 |
C |
A |
6: 122,028,690 (GRCm39) |
D561E |
probably benign |
Het |
| Myo3a |
G |
T |
2: 22,232,383 (GRCm39) |
|
probably benign |
Het |
| N4bp1 |
A |
G |
8: 87,587,165 (GRCm39) |
V591A |
probably damaging |
Het |
| Nckap5 |
C |
T |
1: 125,952,494 (GRCm39) |
G1353R |
probably benign |
Het |
| Or52n3 |
T |
A |
7: 104,530,050 (GRCm39) |
C45* |
probably null |
Het |
| Or5k1b |
G |
T |
16: 58,581,202 (GRCm39) |
C112* |
probably null |
Het |
| Pclo |
T |
C |
5: 14,816,830 (GRCm39) |
S1297P |
|
Het |
| Pkhd1l1 |
T |
C |
15: 44,343,009 (GRCm39) |
I162T |
probably damaging |
Het |
| Plaat5 |
A |
G |
19: 7,591,923 (GRCm39) |
E113G |
probably damaging |
Het |
| Plekhg2 |
T |
C |
7: 28,062,297 (GRCm39) |
E541G |
probably damaging |
Het |
| Ppp1r15a |
T |
C |
7: 45,174,149 (GRCm39) |
N220D |
possibly damaging |
Het |
| Prss3l |
A |
G |
6: 41,420,486 (GRCm39) |
V80A |
probably benign |
Het |
| Psmd4 |
A |
G |
3: 94,940,735 (GRCm39) |
S343P |
probably benign |
Het |
| Rnf213 |
A |
T |
11: 119,354,406 (GRCm39) |
H4063L |
|
Het |
| Rnf40 |
T |
C |
7: 127,190,954 (GRCm39) |
V313A |
probably benign |
Het |
| Ryr2 |
T |
A |
13: 11,752,680 (GRCm39) |
E1880D |
probably benign |
Het |
| Slc30a4 |
A |
T |
2: 122,527,200 (GRCm39) |
L398I |
probably damaging |
Het |
| Sp100 |
A |
T |
1: 85,624,751 (GRCm39) |
N380I |
probably damaging |
Het |
| Speer4d |
T |
G |
5: 15,828,393 (GRCm39) |
L175W |
probably damaging |
Het |
| Srcap |
T |
A |
7: 127,137,273 (GRCm39) |
I985K |
possibly damaging |
Het |
| Tesk2 |
T |
C |
4: 116,658,443 (GRCm39) |
|
probably null |
Het |
| Tgfbrap1 |
A |
G |
1: 43,114,608 (GRCm39) |
V164A |
probably damaging |
Het |
| Tlk1 |
T |
C |
2: 70,544,341 (GRCm39) |
T765A |
probably benign |
Het |
| Tmem207 |
A |
T |
16: 26,345,413 (GRCm39) |
C18S |
|
Het |
| Tph2 |
A |
T |
10: 114,915,992 (GRCm39) |
C394S |
probably benign |
Het |
| Trrap |
C |
A |
5: 144,763,517 (GRCm39) |
H2470N |
probably damaging |
Het |
| Ttc21b |
T |
C |
2: 66,053,866 (GRCm39) |
Y771C |
probably damaging |
Het |
| Uchl3 |
T |
C |
14: 101,904,451 (GRCm39) |
F122L |
probably damaging |
Het |
| Vipr1 |
G |
A |
9: 121,471,993 (GRCm39) |
|
probably null |
Het |
| Vmn2r117 |
G |
T |
17: 23,696,578 (GRCm39) |
D276E |
probably benign |
Het |
| Vps13c |
T |
C |
9: 67,858,674 (GRCm39) |
L2708P |
probably damaging |
Het |
| Vwa3a |
T |
A |
7: 120,385,682 (GRCm39) |
S675T |
possibly damaging |
Het |
| Zfp473 |
A |
G |
7: 44,383,766 (GRCm39) |
L189P |
probably benign |
Het |
| Zfp956 |
T |
A |
6: 47,941,041 (GRCm39) |
C467S |
probably damaging |
Het |
| Zgrf1 |
T |
A |
3: 127,377,741 (GRCm39) |
N995K |
probably benign |
Het |
|
| Other mutations in Nek10 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01361:Nek10
|
APN |
14 |
14,850,957 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL02067:Nek10
|
APN |
14 |
14,861,639 (GRCm38) |
missense |
probably benign |
0.12 |
|
IGL02361:Nek10
|
APN |
14 |
14,843,856 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02687:Nek10
|
APN |
14 |
14,840,570 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02929:Nek10
|
APN |
14 |
14,821,119 (GRCm38) |
missense |
possibly damaging |
0.82 |
|
IGL03229:Nek10
|
APN |
14 |
14,986,686 (GRCm38) |
missense |
probably benign |
0.10 |
|
P0041:Nek10
|
UTSW |
14 |
14,861,603 (GRCm38) |
missense |
probably benign |
0.01 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0142:Nek10
|
UTSW |
14 |
14,861,560 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R0433:Nek10
|
UTSW |
14 |
14,860,927 (GRCm38) |
missense |
probably benign |
0.32 |
|
R0633:Nek10
|
UTSW |
14 |
14,857,782 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1087:Nek10
|
UTSW |
14 |
14,827,059 (GRCm38) |
missense |
possibly damaging |
0.59 |
|
R1184:Nek10
|
UTSW |
14 |
14,931,325 (GRCm38) |
splice site |
probably benign |
|
|
R1250:Nek10
|
UTSW |
14 |
14,853,887 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1371:Nek10
|
UTSW |
14 |
14,850,983 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1506:Nek10
|
UTSW |
14 |
14,999,078 (GRCm38) |
splice site |
probably benign |
|
|
R1829:Nek10
|
UTSW |
14 |
14,863,454 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1831:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1833:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1990:Nek10
|
UTSW |
14 |
14,860,764 (GRCm38) |
missense |
probably benign |
|
|
R1997:Nek10
|
UTSW |
14 |
14,827,003 (GRCm38) |
missense |
probably benign |
0.09 |
|
R2011:Nek10
|
UTSW |
14 |
14,885,122 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2158:Nek10
|
UTSW |
14 |
14,885,047 (GRCm38) |
splice site |
probably null |
|
|
R2288:Nek10
|
UTSW |
14 |
14,853,956 (GRCm38) |
nonsense |
probably null |
|
|
R2568:Nek10
|
UTSW |
14 |
14,999,112 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R2907:Nek10
|
UTSW |
14 |
14,980,613 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R2965:Nek10
|
UTSW |
14 |
14,836,202 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3922:Nek10
|
UTSW |
14 |
14,861,585 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R4032:Nek10
|
UTSW |
14 |
14,853,877 (GRCm38) |
splice site |
probably null |
|
|
R4700:Nek10
|
UTSW |
14 |
14,842,841 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R4742:Nek10
|
UTSW |
14 |
14,861,624 (GRCm38) |
missense |
probably null |
0.03 |
|
R4785:Nek10
|
UTSW |
14 |
14,855,714 (GRCm38) |
missense |
probably benign |
|
|
R4890:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4891:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4920:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4924:Nek10
|
UTSW |
14 |
14,846,594 (GRCm38) |
splice site |
probably null |
|
|
R4928:Nek10
|
UTSW |
14 |
14,930,577 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4948:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4952:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4953:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R5092:Nek10
|
UTSW |
14 |
14,820,851 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R5097:Nek10
|
UTSW |
14 |
14,857,851 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5593:Nek10
|
UTSW |
14 |
14,980,544 (GRCm38) |
nonsense |
probably null |
|
|
R5696:Nek10
|
UTSW |
14 |
14,860,736 (GRCm38) |
splice site |
probably null |
|
|
R5813:Nek10
|
UTSW |
14 |
14,986,704 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5829:Nek10
|
UTSW |
14 |
14,865,404 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5872:Nek10
|
UTSW |
14 |
14,850,896 (GRCm38) |
missense |
probably benign |
0.06 |
|
R5939:Nek10
|
UTSW |
14 |
14,931,290 (GRCm38) |
missense |
possibly damaging |
0.58 |
|
R6025:Nek10
|
UTSW |
14 |
14,865,633 (GRCm38) |
missense |
probably benign |
0.41 |
|
R6235:Nek10
|
UTSW |
14 |
14,821,113 (GRCm38) |
nonsense |
probably null |
|
|
R6539:Nek10
|
UTSW |
14 |
14,860,789 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R6542:Nek10
|
UTSW |
14 |
14,999,108 (GRCm38) |
missense |
probably benign |
0.44 |
|
R6561:Nek10
|
UTSW |
14 |
14,828,448 (GRCm38) |
missense |
possibly damaging |
0.48 |
|
R6659:Nek10
|
UTSW |
14 |
14,861,684 (GRCm38) |
missense |
probably benign |
0.29 |
|
R7039:Nek10
|
UTSW |
14 |
14,986,700 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7039:Nek10
|
UTSW |
14 |
14,826,946 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R7102:Nek10
|
UTSW |
14 |
14,828,517 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7185:Nek10
|
UTSW |
14 |
14,846,621 (GRCm38) |
missense |
probably benign |
0.03 |
|
R7198:Nek10
|
UTSW |
14 |
14,850,947 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7202:Nek10
|
UTSW |
14 |
14,836,171 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7251:Nek10
|
UTSW |
14 |
14,853,965 (GRCm38) |
missense |
probably benign |
|
|
R7345:Nek10
|
UTSW |
14 |
14,955,503 (GRCm38) |
missense |
probably benign |
|
|
R7590:Nek10
|
UTSW |
14 |
15,006,693 (GRCm38) |
makesense |
probably null |
|
|
R7593:Nek10
|
UTSW |
14 |
14,826,955 (GRCm38) |
missense |
probably benign |
0.04 |
|
R7616:Nek10
|
UTSW |
14 |
14,937,759 (GRCm38) |
missense |
probably benign |
0.27 |
|
R7635:Nek10
|
UTSW |
14 |
14,850,932 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7817:Nek10
|
UTSW |
14 |
15,001,017 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7826:Nek10
|
UTSW |
14 |
14,860,846 (GRCm38) |
splice site |
probably null |
|
|
R7986:Nek10
|
UTSW |
14 |
15,001,020 (GRCm38) |
missense |
probably benign |
0.17 |
|
R8765:Nek10
|
UTSW |
14 |
14,999,104 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R8856:Nek10
|
UTSW |
14 |
14,937,610 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8973:Nek10
|
UTSW |
14 |
14,931,321 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9002:Nek10
|
UTSW |
14 |
14,980,590 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9088:Nek10
|
UTSW |
14 |
14,931,314 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9195:Nek10
|
UTSW |
14 |
14,821,139 (GRCm38) |
missense |
probably benign |
0.03 |
|
R9511:Nek10
|
UTSW |
14 |
14,828,511 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9529:Nek10
|
UTSW |
14 |
14,850,833 (GRCm38) |
missense |
probably benign |
|
|
R9590:Nek10
|
UTSW |
14 |
14,853,888 (GRCm38) |
missense |
probably damaging |
1.00 |
|
Z1177:Nek10
|
UTSW |
14 |
15,001,157 (GRCm38) |
nonsense |
probably null |
|
|
Z1177:Nek10
|
UTSW |
14 |
14,853,948 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- GAAGCCCGTCCAGATATTGTAG -3'
(R):5'- TGCTCTGGCTTCACACTAGAC -3'
Sequencing Primer
(F):5'- GCCCGTCCAGATATTGTAGAAGTTAG -3'
(R):5'- TCTGGCTTCACACTAGACAATAAATC -3'
|
| Posted On |
2022-06-15 |
Incidental Mutation