Mutagenetix > Incidental Mutation

Incidental Mutation 'R9477:Gimap4'

715853

Institutional Source

Beutler Lab

Gene Symbol

Gimap4

Ensembl Gene

ENSMUSG00000054435

Gene Name

GTPase, IMAP family member 4

Synonyms

Ian1, E430007K16Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R9477 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

48661483-48668994 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 48667314 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 23 (N23I)

Ref Sequence

ENSEMBL: ENSMUSP00000068398 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067506] [ENSMUST00000090070] [ENSMUST00000118802] [ENSMUST00000119575] [ENSMUST00000121957] [ENSMUST00000156770]

AlphaFold

Q99JY3

Predicted Effect

probably benign
Transcript: ENSMUST00000067506
AA Change: N23I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000068398
Gene: ENSMUSG00000054435
AA Change: N23I

Domain	Start	End	E-Value	Type
Pfam:AIG1	31	218	4.2e-72	PFAM
Pfam:MMR_HSR1	32	186	2.6e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000090070
AA Change: N23I

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000087524
Gene: ENSMUSG00000054435
AA Change: N23I

Domain	Start	End	E-Value	Type
Pfam:AIG1	31	242	1.5e-80	PFAM
Pfam:MMR_HSR1	32	170	1.6e-10	PFAM
low complexity region	265	282	N/A	INTRINSIC

Predicted Effect

SMART Domains

Protein: ENSMUSP00000112530
Gene: ENSMUSG00000054435
AA Change: N23I

Domain	Start	End	E-Value	Type
Pfam:AIG1	31	53	1.6e-7	PFAM
Pfam:AIG1	48	114	6.4e-17	PFAM
low complexity region	137	154	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000119575
AA Change: N23I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000113989
Gene: ENSMUSG00000054435
AA Change: N23I

Domain	Start	End	E-Value	Type
SCOP:d1zin_1	31	50	2e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121957
AA Change: N23I

PolyPhen 2 Score 0.069 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000113016
Gene: ENSMUSG00000054435
AA Change: N23I

Domain	Start	End	E-Value	Type
Pfam:AIG1	31	55	4.3e-8	PFAM
Pfam:AIG1	48	89	1.5e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156770
AA Change: N23I

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000122070
Gene: ENSMUSG00000054435
AA Change: N23I

Domain	Start	End	E-Value	Type
Pfam:AIG1	31	69	6.7e-17	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. This gene exists within a cluster of other related genes located on mouse chromosome 6. This family member encodes a lymphoid signaling protein that functions to accelerate programmed T-cell death, which appears to correlate with the phosphorylation status of the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
PHENOTYPE: Mice homozygous for a knock-out allele show no detectable alterations in T-cell development, selection and activation; however, mutant T cells exhibit a delay in the execution of programmed cell death induced by intrinsic stimuli downstream of caspase-3 activation and phosphatidylserine exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agap1	A	T	1: 89,765,485 (GRCm39)	M618L	probably benign	Het
Anxa2r2	A	T	13: 120,488,282 (GRCm39)	I89N	probably damaging	Het
App	C	A	16: 84,853,392 (GRCm39)	A229S	unknown	Het
B020011L13Rik	G	T	1: 117,728,903 (GRCm39)	E137*	probably null	Het
BC034090	C	T	1: 155,102,087 (GRCm39)	G59E	probably damaging	Het
C1ra	A	G	6: 124,499,455 (GRCm39)	D547G	probably benign	Het
Cdh2	A	T	18: 16,755,212 (GRCm39)	D774E	probably damaging	Het
Cdon	A	G	9: 35,403,201 (GRCm39)	N1104D	probably damaging	Het
Cecr2	G	A	6: 120,720,743 (GRCm39)		probably null	Het
Ces2b	A	T	8: 105,560,556 (GRCm39)	N124Y	probably damaging	Het
Cfap61	A	G	2: 145,985,032 (GRCm39)	T959A	probably benign	Het
Chaf1a	A	T	17: 56,369,244 (GRCm39)	K492*	probably null	Het
Clec14a	A	G	12: 58,314,620 (GRCm39)	V334A	probably benign	Het
Col6a3	A	G	1: 90,706,621 (GRCm39)	I2771T	unknown	Het
Creb3	T	C	4: 43,566,298 (GRCm39)	L244P	probably damaging	Het
Crispld1	T	C	1: 17,816,956 (GRCm39)	V171A	probably benign	Het
Crmp1	T	C	5: 37,446,182 (GRCm39)	Y613H	probably damaging	Het
Csrnp3	G	A	2: 65,852,819 (GRCm39)	V416I	probably benign	Het
Dhrs1	A	G	14: 55,976,868 (GRCm39)	V272A	probably benign	Het
Dip2b	T	C	15: 99,936,784 (GRCm39)	S32P	probably damaging	Het
Dpp10	G	T	1: 123,304,370 (GRCm39)	T510K	possibly damaging	Het
Dst	A	G	1: 34,205,292 (GRCm39)	Y820C	probably damaging	Het
Efcab3	A	T	11: 104,836,698 (GRCm39)	H3562L	unknown	Het
Fam3c	A	T	6: 22,308,479 (GRCm39)	M238K	probably damaging	Het
Fcgbpl1	T	C	7: 27,852,265 (GRCm39)	F1263L	probably damaging	Het
Fcrl2	A	T	3: 87,159,803 (GRCm39)	C484S	probably damaging	Het
Fsd1	T	C	17: 56,295,720 (GRCm39)	L97P	possibly damaging	Het
Gapdhs	T	C	7: 30,431,682 (GRCm39)	I310V	probably damaging	Het
Gpr21	T	A	2: 37,408,386 (GRCm39)	F311I	probably damaging	Het
Grm1	T	C	10: 10,595,405 (GRCm39)	E741G	probably benign	Het
Gsto2	G	A	19: 47,864,911 (GRCm39)	R148H	probably benign	Het
Gzmd	A	G	14: 56,368,813 (GRCm39)	V27A	probably benign	Het
Hbp1	A	G	12: 31,980,766 (GRCm39)	C446R	probably damaging	Het
Hmcn2	A	T	2: 31,286,031 (GRCm39)		probably null	Het
Ifi205	G	A	1: 173,854,155 (GRCm39)	T166I	probably benign	Het
Il23a	T	A	10: 128,133,922 (GRCm39)		probably benign	Het
Itpr3	G	A	17: 27,337,651 (GRCm39)		probably benign	Het
Jag1	A	T	2: 136,936,409 (GRCm39)	C362S	probably damaging	Het
Jmjd1c	A	T	10: 66,993,734 (GRCm39)	R72*	probably null	Het
Kcna6	A	G	6: 126,716,361 (GRCm39)	I176T	probably damaging	Het
Marchf1	T	C	8: 66,871,542 (GRCm39)	V102A	probably damaging	Het
Mast1	T	C	8: 85,638,779 (GRCm39)	K1517E	probably benign	Het
Mbnl1	A	G	3: 60,520,769 (GRCm39)	D119G	probably damaging	Het
Mfsd8	A	G	3: 40,785,057 (GRCm39)		probably null	Het
Mtus2	T	G	5: 148,014,740 (GRCm39)	V511G	probably benign	Het
Myot	A	C	18: 44,470,333 (GRCm39)	Q103H	probably benign	Het
Nrip2	G	A	6: 128,377,145 (GRCm39)	D24N	probably benign	Het
Ntm	T	A	9: 29,322,922 (GRCm39)	S33C	probably benign	Het
Nus1	C	T	10: 52,294,001 (GRCm39)	T55M	possibly damaging	Het
Or4c112	G	A	2: 88,853,615 (GRCm39)	T244I	probably benign	Het
Pik3cb	C	T	9: 98,922,973 (GRCm39)		probably null	Het
Pogk	T	C	1: 166,227,404 (GRCm39)	N249S	probably damaging	Het
Pou1f1	C	T	16: 65,320,503 (GRCm39)	T66I	possibly damaging	Het
Ppp1r42	T	G	1: 10,055,854 (GRCm39)	K211N	probably damaging	Het
Ppp2r3d	C	A	9: 124,476,857 (GRCm38)	D2Y		Het
Prdm5	A	T	6: 65,771,342 (GRCm39)	N51I	possibly damaging	Het
Rif1	G	A	2: 52,001,342 (GRCm39)	A1599T	probably benign	Het
Sema3c	G	A	5: 17,921,981 (GRCm39)	A520T		Het
Shank1	G	A	7: 43,962,342 (GRCm39)	S71N	unknown	Het
Spice1	T	A	16: 44,197,183 (GRCm39)	N489K	possibly damaging	Het
Sptbn4	A	G	7: 27,132,624 (GRCm39)	M116T	possibly damaging	Het
Supt5	T	A	7: 28,025,500 (GRCm39)	N249Y	probably damaging	Het
Tex2	A	G	11: 106,410,706 (GRCm39)		probably null	Het
Trhde	T	A	10: 114,338,980 (GRCm39)	E610V	probably benign	Het
Trmt12	T	C	15: 58,745,471 (GRCm39)	S290P	possibly damaging	Het
Trpm2	C	T	10: 77,747,224 (GRCm39)	V1425M	probably benign	Het
Uaca	T	C	9: 60,778,108 (GRCm39)	S832P	probably benign	Het
Vcan	T	A	13: 89,841,128 (GRCm39)	D1472V	probably damaging	Het
Virma	A	G	4: 11,528,753 (GRCm39)	E1330G	probably damaging	Het
Vmn1r125	T	G	7: 21,006,261 (GRCm39)	V53G	possibly damaging	Het
Vmn2r27	A	T	6: 124,168,910 (GRCm39)	I740N	probably damaging	Het
Vmn2r70	C	T	7: 85,218,244 (GRCm39)	C18Y	possibly damaging	Het
Zfp35	A	G	18: 24,136,188 (GRCm39)	I177M	possibly damaging	Het
Zfp532	T	C	18: 65,777,428 (GRCm39)	I895T	probably benign	Het
Zfp58	C	A	13: 67,640,158 (GRCm39)	C111F	probably damaging	Het

Other mutations in Gimap4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00980:Gimap4	APN	6	48,667,872 (GRCm39)	missense	probably damaging	1.00
IGL01917:Gimap4	APN	6	48,667,854 (GRCm39)	missense	probably benign	0.02
IGL02302:Gimap4	APN	6	48,667,347 (GRCm39)	missense	probably damaging	1.00
IGL02679:Gimap4	APN	6	48,667,429 (GRCm39)	nonsense	probably null
R1466:Gimap4	UTSW	6	48,668,216 (GRCm39)	missense	probably benign	0.17
R1466:Gimap4	UTSW	6	48,668,216 (GRCm39)	missense	probably benign	0.17
R1584:Gimap4	UTSW	6	48,668,216 (GRCm39)	missense	probably benign	0.17
R2079:Gimap4	UTSW	6	48,667,881 (GRCm39)	missense	possibly damaging	0.46
R2118:Gimap4	UTSW	6	48,667,905 (GRCm39)	missense	probably benign	0.24
R2566:Gimap4	UTSW	6	48,667,799 (GRCm39)	missense	probably damaging	1.00
R4279:Gimap4	UTSW	6	48,667,511 (GRCm39)	missense	probably benign	0.22
R5592:Gimap4	UTSW	6	48,668,092 (GRCm39)	missense	probably damaging	0.99
R5597:Gimap4	UTSW	6	48,667,698 (GRCm39)	missense	probably damaging	1.00
R6162:Gimap4	UTSW	6	48,667,655 (GRCm39)	missense	probably damaging	0.97
R6354:Gimap4	UTSW	6	48,663,814 (GRCm39)	missense	possibly damaging	0.53
R6658:Gimap4	UTSW	6	48,668,338 (GRCm39)	missense	possibly damaging	0.65
R8028:Gimap4	UTSW	6	48,667,684 (GRCm39)	missense	probably damaging	0.98
R8349:Gimap4	UTSW	6	48,667,694 (GRCm39)	missense	probably damaging	1.00
R8449:Gimap4	UTSW	6	48,667,694 (GRCm39)	missense	probably damaging	1.00
R8993:Gimap4	UTSW	6	48,667,539 (GRCm39)	missense	probably damaging	1.00
R9112:Gimap4	UTSW	6	48,667,629 (GRCm39)	missense	probably benign	0.00
R9366:Gimap4	UTSW	6	48,668,037 (GRCm39)	missense	probably benign
R9367:Gimap4	UTSW	6	48,667,746 (GRCm39)	missense	probably damaging	1.00
X0050:Gimap4	UTSW	6	48,667,734 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- GACAGGCATTTAGACCCACAGG -3'
(R):5'- CAGCATCTGGTACCTCAGTGTC -3'

Sequencing Primer
(F):5'- CAGGTTGTCTGTAGTCCCTG -3'
(R):5'- CTCAGTGTCAAAAATACCAGGTG -3'

Posted On

2022-06-15