Incidental Mutation 'R9484:Eif3a'
ID 716491
Institutional Source Beutler Lab
Gene Symbol Eif3a
Ensembl Gene ENSMUSG00000024991
Gene Name eukaryotic translation initiation factor 3, subunit A
Synonyms Eif3, Csma, Eif3s10, A830012B05Rik
MMRRC Submission
Accession Numbers
Essential gene? Probably essential (E-score: 0.971) question?
Stock # R9484 (G1)
Quality Score 225.009
Status Not validated
Chromosome 19
Chromosomal Location 60749555-60779096 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 60755006 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Serine to Threonine at position 1059 (S1059T)
Ref Sequence ENSEMBL: ENSMUSP00000025955 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025955]
AlphaFold P23116
Predicted Effect unknown
Transcript: ENSMUST00000025955
AA Change: S1059T
SMART Domains Protein: ENSMUSP00000025955
Gene: ENSMUSG00000024991
AA Change: S1059T

DomainStartEndE-ValueType
coiled coil region 82 120 N/A INTRINSIC
PINT 426 506 5.69e-18 SMART
SCOP:d1f5aa2 563 711 5e-3 SMART
coiled coil region 772 880 N/A INTRINSIC
low complexity region 919 947 N/A INTRINSIC
low complexity region 951 976 N/A INTRINSIC
internal_repeat_2 978 991 1.01e-8 PROSPERO
low complexity region 993 1007 N/A INTRINSIC
low complexity region 1013 1027 N/A INTRINSIC
low complexity region 1033 1057 N/A INTRINSIC
low complexity region 1064 1089 N/A INTRINSIC
internal_repeat_1 1090 1111 6.2e-12 PROSPERO
internal_repeat_2 1099 1112 1.01e-8 PROSPERO
internal_repeat_1 1110 1131 6.2e-12 PROSPERO
low complexity region 1146 1173 N/A INTRINSIC
low complexity region 1176 1206 N/A INTRINSIC
low complexity region 1221 1260 N/A INTRINSIC
low complexity region 1265 1297 N/A INTRINSIC
low complexity region 1301 1314 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.8%
  • 10x: 99.2%
  • 20x: 97.1%
Validation Efficiency
Allele List at MGI

All alleles(36) : Targeted, other(2) Gene trapped(34)

Other mutations in this stock
Total: 83 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acads A T 5: 115,250,845 (GRCm39) C151S probably damaging Het
Actr8 T C 14: 29,708,301 (GRCm39) I193T probably benign Het
Btbd2 T C 10: 80,480,103 (GRCm39) N419S probably benign Het
Cacna2d1 T A 5: 16,561,831 (GRCm39) W821R probably damaging Het
Cadps2 T C 6: 23,626,646 (GRCm39) Y214C probably benign Het
Calu T A 6: 29,366,162 (GRCm39) L180Q probably damaging Het
Corin C T 5: 72,497,280 (GRCm39) V615I probably damaging Het
Cyp8b1 A T 9: 121,744,983 (GRCm39) D116E probably benign Het
D630036H23Rik C A 12: 36,431,711 (GRCm39) A96S unknown Het
Ddx24 A T 12: 103,377,555 (GRCm39) Y717N probably damaging Het
Dmap1 G T 4: 117,533,308 (GRCm39) Q249K probably benign Het
Dnah10 T A 5: 124,900,508 (GRCm39) W3922R probably damaging Het
Dnah14 T C 1: 181,517,773 (GRCm39) F2036L probably benign Het
Dnah14 T C 1: 181,625,311 (GRCm39) I4064T probably benign Het
Dock9 A C 14: 121,818,844 (GRCm39) V1546G probably damaging Het
Eif1ad4 A T 12: 87,862,249 (GRCm39) Q37L possibly damaging Het
Ep300 A G 15: 81,521,026 (GRCm39) E1262G unknown Het
Evl T A 12: 108,652,716 (GRCm39) I387N probably damaging Het
Fat2 A C 11: 55,200,752 (GRCm39) V774G probably damaging Het
Fez1 T A 9: 36,755,093 (GRCm39) Y31N probably benign Het
Fkbp10 A G 11: 100,313,960 (GRCm39) I435V probably damaging Het
Flnb A G 14: 7,929,004 (GRCm38) D1911G probably benign Het
Fnbp1 T C 2: 30,973,038 (GRCm39) Y154C probably benign Het
Fndc10 T C 4: 155,779,496 (GRCm39) I180T possibly damaging Het
Frmd4a G A 2: 4,609,026 (GRCm39) V965I possibly damaging Het
Gabrr2 A G 4: 33,071,352 (GRCm39) H64R possibly damaging Het
Glis3 T C 19: 28,508,403 (GRCm39) D527G probably damaging Het
H2-Ob T A 17: 34,459,989 (GRCm39) F33L probably damaging Het
Hbb-bh1 C T 7: 103,492,239 (GRCm39) E27K probably benign Het
Ifnb1 T A 4: 88,440,915 (GRCm39) T33S probably benign Het
Igkv4-80 T A 6: 68,993,766 (GRCm39) T42S probably damaging Het
Irs2 C T 8: 11,057,334 (GRCm39) G366D probably damaging Het
Kcnk2 CAAA CAA 1: 188,988,891 (GRCm39) probably null Het
Krtap5-3 T A 7: 141,756,068 (GRCm39) C302S unknown Het
Lgi1 A G 19: 38,294,757 (GRCm39) K510E probably benign Het
Lgi2 T A 5: 52,695,936 (GRCm39) D341V probably benign Het
Lin7c T C 2: 109,724,813 (GRCm39) I14T probably benign Het
Lrrc19 A T 4: 94,531,573 (GRCm39) M13K probably benign Het
Lrrc69 T C 4: 14,666,012 (GRCm39) I315M probably benign Het
Mtcl3 G A 10: 29,072,969 (GRCm39) D754N probably damaging Het
Myo5c A T 9: 75,204,770 (GRCm39) D1541V probably damaging Het
Mypn T G 10: 63,003,019 (GRCm39) M373L probably benign Het
Nckipsd C A 9: 108,689,837 (GRCm39) H333N probably damaging Het
Nrg2 A T 18: 36,157,401 (GRCm39) L428Q probably null Het
Or13a20 T C 7: 140,231,904 (GRCm39) L4S probably benign Het
Or2b28 C A 13: 21,531,587 (GRCm39) T163K probably damaging Het
Or5an11 T C 19: 12,245,735 (GRCm39) I47T possibly damaging Het
Otogl T A 10: 107,657,894 (GRCm39) probably null Het
Otogl C T 10: 107,737,156 (GRCm39) G86D probably damaging Het
Papln A T 12: 83,838,618 (GRCm39) Q1249L probably benign Het
Plxna2 G T 1: 194,327,202 (GRCm39) G379C probably damaging Het
Pofut2 T C 10: 77,095,260 (GRCm39) I35T probably benign Het
Pros1 A G 16: 62,744,887 (GRCm39) T501A possibly damaging Het
Rapgef1 T C 2: 29,625,821 (GRCm39) S1042P possibly damaging Het
Rps6kb1 C T 11: 86,408,443 (GRCm39) E185K probably damaging Het
Slc13a2 A T 11: 78,294,233 (GRCm39) L216Q probably damaging Het
Slc22a14 T C 9: 119,009,615 (GRCm39) Y160C probably damaging Het
Slc22a4 T A 11: 53,879,773 (GRCm39) I429F possibly damaging Het
Slc26a3 A G 12: 31,511,785 (GRCm39) K457E probably damaging Het
Slc47a2 T C 11: 61,227,060 (GRCm39) I169M possibly damaging Het
Slco1a1 C T 6: 141,854,672 (GRCm39) V660I probably benign Het
Smyd1 A G 6: 71,202,450 (GRCm39) Y252H probably damaging Het
Spp2 A T 1: 88,334,695 (GRCm39) probably benign Het
Stra8 T A 6: 34,911,121 (GRCm39) W250R probably damaging Het
Syne1 A C 10: 5,170,359 (GRCm39) L5183R probably damaging Het
Tdrd9 T C 12: 112,012,684 (GRCm39) S1203P probably damaging Het
Th C A 7: 142,453,620 (GRCm39) E27* probably null Het
Thada C A 17: 84,736,619 (GRCm39) L887F probably damaging Het
Timm23 T C 14: 31,902,586 (GRCm39) T186A probably benign Het
Tmem132b A T 5: 125,860,420 (GRCm39) E555V probably damaging Het
Tnik A T 3: 28,649,093 (GRCm39) Q457L unknown Het
Uba7 C A 9: 107,861,037 (GRCm39) H942Q probably benign Het
Upf2 T C 2: 5,966,078 (GRCm39) S233P unknown Het
Urm1 T A 2: 29,732,760 (GRCm39) N72K probably damaging Het
Usp10 T C 8: 120,675,504 (GRCm39) S508P possibly damaging Het
Usp37 G A 1: 74,499,081 (GRCm39) P632S probably damaging Het
Wdr77 T A 3: 105,872,404 (GRCm39) N209K probably benign Het
Zbtb24 C T 10: 41,327,429 (GRCm39) T105M probably benign Het
Zfp341 T A 2: 154,485,763 (GRCm39) I671N probably damaging Het
Zfp414 A G 17: 33,848,984 (GRCm39) T73A probably benign Het
Zfp574 A T 7: 24,781,404 (GRCm39) I809F possibly damaging Het
Zfp62 T C 11: 49,108,108 (GRCm39) I733T probably damaging Het
Zfp719 A G 7: 43,239,581 (GRCm39) I390V possibly damaging Het
Other mutations in Eif3a
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00485:Eif3a APN 19 60,758,328 (GRCm39) missense unknown
IGL00981:Eif3a APN 19 60,755,049 (GRCm39) missense unknown
IGL01650:Eif3a APN 19 60,762,434 (GRCm39) missense probably damaging 1.00
IGL01926:Eif3a APN 19 60,758,399 (GRCm39) missense unknown
IGL02100:Eif3a APN 19 60,755,442 (GRCm39) splice site probably benign
IGL02316:Eif3a APN 19 60,760,076 (GRCm39) splice site probably benign
IGL02444:Eif3a APN 19 60,762,045 (GRCm39) missense possibly damaging 0.89
IGL02552:Eif3a APN 19 60,751,664 (GRCm39) unclassified probably benign
IGL02797:Eif3a APN 19 60,761,164 (GRCm39) missense probably damaging 1.00
IGL03108:Eif3a APN 19 60,770,747 (GRCm39) missense possibly damaging 0.85
FR4304:Eif3a UTSW 19 60,763,728 (GRCm39) critical splice donor site probably benign
FR4548:Eif3a UTSW 19 60,763,729 (GRCm39) critical splice donor site probably benign
FR4737:Eif3a UTSW 19 60,763,727 (GRCm39) critical splice donor site probably benign
FR4976:Eif3a UTSW 19 60,763,729 (GRCm39) critical splice donor site probably benign
G5538:Eif3a UTSW 19 60,770,340 (GRCm39) missense probably damaging 0.97
R0054:Eif3a UTSW 19 60,755,264 (GRCm39) missense unknown
R0054:Eif3a UTSW 19 60,755,264 (GRCm39) missense unknown
R1483:Eif3a UTSW 19 60,757,164 (GRCm39) missense unknown
R1636:Eif3a UTSW 19 60,770,343 (GRCm39) missense possibly damaging 0.93
R1748:Eif3a UTSW 19 60,755,236 (GRCm39) missense unknown
R1857:Eif3a UTSW 19 60,770,635 (GRCm39) missense probably damaging 1.00
R1858:Eif3a UTSW 19 60,770,635 (GRCm39) missense probably damaging 1.00
R1993:Eif3a UTSW 19 60,769,954 (GRCm39) missense probably benign 0.19
R2034:Eif3a UTSW 19 60,750,568 (GRCm39) unclassified probably benign
R2099:Eif3a UTSW 19 60,752,551 (GRCm39) unclassified probably benign
R2140:Eif3a UTSW 19 60,763,832 (GRCm39) splice site probably benign
R2434:Eif3a UTSW 19 60,752,488 (GRCm39) unclassified probably benign
R2940:Eif3a UTSW 19 60,762,115 (GRCm39) missense probably benign 0.22
R4630:Eif3a UTSW 19 60,766,424 (GRCm39) missense probably benign 0.41
R4630:Eif3a UTSW 19 60,758,366 (GRCm39) missense unknown
R4926:Eif3a UTSW 19 60,751,656 (GRCm39) unclassified probably benign
R5366:Eif3a UTSW 19 60,767,971 (GRCm39) missense probably benign 0.12
R6003:Eif3a UTSW 19 60,755,319 (GRCm39) missense unknown
R6082:Eif3a UTSW 19 60,760,568 (GRCm39) missense possibly damaging 0.82
R6256:Eif3a UTSW 19 60,759,464 (GRCm39) missense possibly damaging 0.72
R7056:Eif3a UTSW 19 60,751,500 (GRCm39) splice site probably null
R7365:Eif3a UTSW 19 60,755,082 (GRCm39) missense unknown
R7922:Eif3a UTSW 19 60,764,280 (GRCm39) missense probably damaging 1.00
R8076:Eif3a UTSW 19 60,762,363 (GRCm39) missense probably damaging 0.97
R8169:Eif3a UTSW 19 60,750,628 (GRCm39) missense unknown
R8246:Eif3a UTSW 19 60,767,806 (GRCm39) missense probably damaging 1.00
R8474:Eif3a UTSW 19 60,767,929 (GRCm39) missense possibly damaging 0.63
R8546:Eif3a UTSW 19 60,755,208 (GRCm39) missense unknown
R8964:Eif3a UTSW 19 60,751,630 (GRCm39) missense unknown
R9071:Eif3a UTSW 19 60,751,634 (GRCm39) missense unknown
R9290:Eif3a UTSW 19 60,765,221 (GRCm39) missense probably damaging 1.00
R9780:Eif3a UTSW 19 60,766,398 (GRCm39) missense probably damaging 1.00
X0028:Eif3a UTSW 19 60,770,340 (GRCm39) missense probably damaging 0.97
X0066:Eif3a UTSW 19 60,750,731 (GRCm39) unclassified probably benign
Predicted Primers PCR Primer
(F):5'- GCATCATCACCCCTTCTAGG -3'
(R):5'- GCAGATGATGATAGGCCTCC -3'

Sequencing Primer
(F):5'- AGGAACCCTATCATCATCCATATTTC -3'
(R):5'- CTCCCAGGCGAATTGGTGATG -3'
Posted On 2022-07-18