Mutagenetix > Incidental Mutation

Incidental Mutation 'R9485:Zfp985'

716506

Institutional Source

Beutler Lab

Gene Symbol

Zfp985

Ensembl Gene

ENSMUSG00000065999

Gene Name

zinc finger protein 985

Synonyms

Gm13154

MMRRC Submission

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.624) question?

Stock #

R9485 (G1)

Quality Score

100.008

Status

Not validated

Chromosome

Chromosomal Location

147637734-147669655 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 147668280 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 383 (C383S)

Ref Sequence

ENSEMBL: ENSMUSP00000080438 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081742] [ENSMUST00000139784] [ENSMUST00000143885]

AlphaFold

A2A7A5

Predicted Effect

probably damaging
Transcript: ENSMUST00000081742
AA Change: C383S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000080438
Gene: ENSMUSG00000065999
AA Change: C383S

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART
ZnF_C2H2	238	260	8.34e-3	SMART
ZnF_C2H2	266	288	1.47e-3	SMART
ZnF_C2H2	294	316	2.36e-2	SMART
ZnF_C2H2	322	344	8.34e-3	SMART
ZnF_C2H2	350	372	7.67e-2	SMART
ZnF_C2H2	378	400	8.6e-5	SMART
ZnF_C2H2	406	428	8.6e-5	SMART
ZnF_C2H2	434	456	7.9e-4	SMART
ZnF_C2H2	462	484	1.95e-3	SMART
ZnF_C2H2	490	512	2.09e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000139784

SMART Domains

Protein: ENSMUSP00000123296
Gene: ENSMUSG00000065999

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000143885

SMART Domains

Protein: ENSMUSP00000121177
Gene: ENSMUSG00000065999

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	T	C	14: 32,383,400 (GRCm39)	D855G	possibly damaging	Het
4930486L24Rik	A	T	13: 61,001,059 (GRCm39)	V159D	possibly damaging	Het
Ahnak	G	A	19: 8,979,438 (GRCm39)	A241T	probably benign	Het
Apoa4	T	C	9: 46,152,453 (GRCm39)	M1T	probably null	Het
Atn1	T	C	6: 124,722,748 (GRCm39)	K776E	unknown	Het
Atp1a2	A	G	1: 172,105,822 (GRCm39)	*948R	probably null	Het
Atp7b	T	C	8: 22,502,778 (GRCm39)	Q801R	probably damaging	Het
Birc6	T	A	17: 74,945,398 (GRCm39)	S2824T	probably damaging	Het
Cacng3	A	T	7: 122,361,435 (GRCm39)	I109F	probably damaging	Het
Cass4	T	C	2: 172,269,805 (GRCm39)	F629S	probably benign	Het
Ccdc168	T	G	1: 44,095,399 (GRCm39)	K1900Q	possibly damaging	Het
Cnot6l	T	C	5: 96,230,858 (GRCm39)	T370A	probably damaging	Het
Cntnap5c	A	C	17: 58,409,103 (GRCm39)	D447A	probably damaging	Het
Col11a2	T	A	17: 34,258,669 (GRCm39)	L14Q	unknown	Het
Dennd4a	T	G	9: 64,814,388 (GRCm39)	Y1505*	probably null	Het
Dhx32	T	C	7: 133,327,110 (GRCm39)	M464V	possibly damaging	Het
Dip2b	T	C	15: 100,052,924 (GRCm39)	V266A	probably benign	Het
Dnajb6	C	T	5: 29,986,517 (GRCm39)	Q220*	probably null	Het
Dnmt3a	G	A	12: 3,916,121 (GRCm39)	S102N	probably benign	Het
Dph5	A	T	3: 115,681,977 (GRCm39)		probably benign	Het
Ear6	T	A	14: 52,091,489 (GRCm39)	L12H		Het
Erp27	T	C	6: 136,886,548 (GRCm39)	T162A	possibly damaging	Het
Fance	T	A	17: 28,536,479 (GRCm39)	L13H	probably damaging	Het
Fanci	T	A	7: 79,089,405 (GRCm39)	V947D	probably benign	Het
Gab1	G	A	8: 81,515,484 (GRCm39)	T278M	probably damaging	Het
Gbp4	A	T	5: 105,269,796 (GRCm39)	M344K	probably damaging	Het
Gpr87	C	T	3: 59,087,005 (GRCm39)	V167M	possibly damaging	Het
Gramd1a	T	G	7: 30,829,963 (GRCm39)	D708A	unknown	Het
Gzmd	T	A	14: 56,368,160 (GRCm39)	I100F	probably benign	Het
Hcrtr1	T	A	4: 130,031,054 (GRCm39)	M77L	possibly damaging	Het
Hipk2	C	A	6: 38,680,445 (GRCm39)	R965L	possibly damaging	Het
Ift88	T	C	14: 57,675,724 (GRCm39)	M79T	probably benign	Het
Ighv1-12	T	C	12: 114,579,525 (GRCm39)	Y99C	possibly damaging	Het
Il1rl2	CTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATT	CTTTATTTTATTTTATTTTATTTTATTTTATTTTATT	1: 40,366,470 (GRCm39)		probably benign	Het
Il22b	A	G	10: 118,130,314 (GRCm39)	V63A	probably benign	Het
Mllt1	C	T	17: 57,207,184 (GRCm39)	R220H	probably damaging	Het
Mroh8	T	C	2: 157,071,913 (GRCm39)	T531A	probably benign	Het
Myh6	T	C	14: 55,181,802 (GRCm39)	K1833R	probably benign	Het
Nfkbie	C	T	17: 45,871,353 (GRCm39)	T270I	probably damaging	Het
Or4c110	G	A	2: 88,831,709 (GRCm39)	P308S	unknown	Het
Or52b3	A	T	7: 102,204,013 (GRCm39)	N174I	probably damaging	Het
Or52e5	C	T	7: 104,718,703 (GRCm39)	H10Y	possibly damaging	Het
Pcdh8	A	T	14: 80,005,689 (GRCm39)	F900I	probably damaging	Het
Pcolce2	T	A	9: 95,520,720 (GRCm39)	C32*	probably null	Het
Pus7	A	T	5: 23,973,859 (GRCm39)	S212T	probably benign	Het
Rgs12	G	T	5: 35,189,614 (GRCm39)	W1322L	probably damaging	Het
Sec1	G	A	7: 45,328,033 (GRCm39)	T338I	probably damaging	Het
Slc25a36	T	C	9: 96,962,522 (GRCm39)	K156E	probably benign	Het
Snrpn	C	T	7: 59,637,212 (GRCm39)	D35N	probably damaging	Het
Sox9	A	G	11: 112,673,705 (GRCm39)	S99G	probably benign	Het
Spmip3	T	C	1: 177,580,545 (GRCm39)	V130A	possibly damaging	Het
Tacc3	T	C	5: 33,821,644 (GRCm39)	S135P	possibly damaging	Het
Taf2	T	C	15: 54,911,667 (GRCm39)	E583G	probably benign	Het
Tmprss7	T	A	16: 45,498,282 (GRCm39)	K366*	probably null	Het
Trpm6	G	T	19: 18,755,978 (GRCm39)	V74L	probably benign	Het
Ttbk2	G	T	2: 120,575,986 (GRCm39)	T997N	probably benign	Het
Utp25	C	A	1: 192,812,541 (GRCm39)		probably benign	Het
Vmn2r3	T	A	3: 64,183,046 (GRCm39)	I218F	probably damaging	Het
Vps50	T	C	6: 3,592,557 (GRCm39)	V730A	probably damaging	Het
Wdr47	A	T	3: 108,544,371 (GRCm39)	I665F	probably damaging	Het
Wnt7a	T	A	6: 91,343,297 (GRCm39)	N195I	probably benign	Het
Zfp131	A	G	13: 120,251,885 (GRCm39)		probably benign	Het
Zfp709	T	A	8: 72,643,669 (GRCm39)	V366E	possibly damaging	Het

Other mutations in Zfp985

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0098:Zfp985	UTSW	4	147,661,566 (GRCm39)	missense	probably damaging	0.97
R0324:Zfp985	UTSW	4	147,667,314 (GRCm39)	missense	probably benign	0.00
R1307:Zfp985	UTSW	4	147,667,704 (GRCm39)	missense	probably benign
R1594:Zfp985	UTSW	4	147,667,537 (GRCm39)	missense	probably benign	0.05
R1657:Zfp985	UTSW	4	147,668,567 (GRCm39)	missense	probably benign	0.01
R1667:Zfp985	UTSW	4	147,668,407 (GRCm39)	missense	possibly damaging	0.84
R1761:Zfp985	UTSW	4	147,668,502 (GRCm39)	missense	probably benign	0.00
R1858:Zfp985	UTSW	4	147,667,315 (GRCm39)	missense	probably benign	0.29
R2509:Zfp985	UTSW	4	147,667,443 (GRCm39)	missense	possibly damaging	0.75
R2510:Zfp985	UTSW	4	147,667,443 (GRCm39)	missense	possibly damaging	0.75
R2847:Zfp985	UTSW	4	147,667,468 (GRCm39)	nonsense	probably null
R2848:Zfp985	UTSW	4	147,667,468 (GRCm39)	nonsense	probably null
R4245:Zfp985	UTSW	4	147,667,396 (GRCm39)	missense	probably damaging	0.96
R4260:Zfp985	UTSW	4	147,668,029 (GRCm39)	missense	probably damaging	1.00
R4434:Zfp985	UTSW	4	147,668,368 (GRCm39)	missense	probably benign	0.37
R4480:Zfp985	UTSW	4	147,668,536 (GRCm39)	missense	probably benign	0.07
R4512:Zfp985	UTSW	4	147,668,020 (GRCm39)	missense	probably damaging	1.00
R4514:Zfp985	UTSW	4	147,668,020 (GRCm39)	missense	probably damaging	1.00
R4528:Zfp985	UTSW	4	147,667,347 (GRCm39)	missense	possibly damaging	0.49
R4836:Zfp985	UTSW	4	147,668,612 (GRCm39)	missense	probably damaging	0.97
R4884:Zfp985	UTSW	4	147,667,801 (GRCm39)	missense	probably benign	0.04
R5054:Zfp985	UTSW	4	147,667,438 (GRCm39)	missense	probably damaging	0.98
R5106:Zfp985	UTSW	4	147,668,612 (GRCm39)	missense	probably damaging	0.97
R5205:Zfp985	UTSW	4	147,667,368 (GRCm39)	missense	probably damaging	1.00
R5266:Zfp985	UTSW	4	147,667,289 (GRCm39)	critical splice acceptor site	probably null
R5468:Zfp985	UTSW	4	147,667,702 (GRCm39)	missense	probably benign
R5533:Zfp985	UTSW	4	147,667,440 (GRCm39)	nonsense	probably null
R6282:Zfp985	UTSW	4	147,667,805 (GRCm39)	missense	probably benign	0.00
R6303:Zfp985	UTSW	4	147,668,232 (GRCm39)	missense	probably benign	0.01
R6609:Zfp985	UTSW	4	147,668,124 (GRCm39)	missense	probably damaging	1.00
R6609:Zfp985	UTSW	4	147,667,578 (GRCm39)	missense	probably benign
R6722:Zfp985	UTSW	4	147,667,528 (GRCm39)	missense	probably benign	0.26
R6858:Zfp985	UTSW	4	147,667,764 (GRCm39)	nonsense	probably null
R7064:Zfp985	UTSW	4	147,667,573 (GRCm39)	missense	probably benign	0.20
R7216:Zfp985	UTSW	4	147,667,913 (GRCm39)	missense	probably damaging	1.00
R7471:Zfp985	UTSW	4	147,667,388 (GRCm39)	missense	possibly damaging	0.75
R7583:Zfp985	UTSW	4	147,667,946 (GRCm39)	nonsense	probably null
R7685:Zfp985	UTSW	4	147,667,331 (GRCm39)	missense	probably benign	0.00
R8242:Zfp985	UTSW	4	147,668,639 (GRCm39)	missense	possibly damaging	0.52
R8504:Zfp985	UTSW	4	147,667,883 (GRCm39)	missense	possibly damaging	0.70
R8780:Zfp985	UTSW	4	147,668,412 (GRCm39)	missense	possibly damaging	0.79
R8785:Zfp985	UTSW	4	147,668,080 (GRCm39)	missense	probably damaging	1.00
R9513:Zfp985	UTSW	4	147,667,999 (GRCm39)	missense	probably damaging	1.00
R9631:Zfp985	UTSW	4	147,665,742 (GRCm39)	missense	probably damaging	1.00
R9722:Zfp985	UTSW	4	147,667,618 (GRCm39)	missense	possibly damaging	0.63
R9786:Zfp985	UTSW	4	147,668,047 (GRCm39)	missense	probably benign
X0050:Zfp985	UTSW	4	147,667,728 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- TTCATACAGGAGAGAAACCTCACA -3'
(R):5'- CTCCTGTATGAATTCCCTGATGAA -3'

Sequencing Primer
(F):5'- GTGAATGTGACAAATGCTTTACCCAC -3'
(R):5'- AGACTGCCTTTTCCAGTAAAGC -3'

Posted On

2022-07-18