Mutagenetix > Incidental Mutation

Incidental Mutation 'R9521:Nxt2'

718919

Institutional Source

Beutler Lab

Gene Symbol

Nxt2

Ensembl Gene

ENSMUSG00000042271

Gene Name

nuclear transport factor 2-like export factor 2

Synonyms

P15-2, 6330587F24Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.310) question?

Stock #

R9521 (G1)

Quality Score

221.999

Status

Not validated

Chromosome

Chromosomal Location

141009766-141022688 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 141020747 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 118 (A118V)

Ref Sequence

ENSEMBL: ENSMUSP00000108536 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042329] [ENSMUST00000112913] [ENSMUST00000112914] [ENSMUST00000112916]

AlphaFold

Q3UNA4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000042329
AA Change: A118V

PolyPhen 2 Score 0.779 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000048221
Gene: ENSMUSG00000042271
AA Change: A118V

Domain	Start	End	E-Value	Type
Pfam:NTF2	17	136	3.5e-31	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112913
AA Change: A90V

PolyPhen 2 Score 0.779 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000108535
Gene: ENSMUSG00000042271
AA Change: A90V

Domain	Start	End	E-Value	Type
Pfam:NTF2	1	108	1.4e-25	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112914
AA Change: A118V

PolyPhen 2 Score 0.779 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000108536
Gene: ENSMUSG00000042271
AA Change: A118V

Domain	Start	End	E-Value	Type
Pfam:NTF2	17	136	3.5e-31	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112916
AA Change: A174V

PolyPhen 2 Score 0.754 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000108538
Gene: ENSMUSG00000042271
AA Change: A174V

Domain	Start	End	E-Value	Type
Pfam:NTF2	73	192	1.5e-31	PFAM

Meta Mutation Damage Score

0.4261

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene contains a nuclear transport factor 2 (NTF2) domain, which plays an important role in the trafficking of macromolecules, ions, and small molecules between the cytoplasm and nucleus. This protein may also have a role in mRNA nuclear export. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jun 2011]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	T	C	13: 119,608,778 (GRCm39)		probably null	Het
A030005K14Rik	C	A	1: 83,036,980 (GRCm39)	G17V	unknown	Het
Afap1l1	C	T	18: 61,879,863 (GRCm39)	A336T	probably benign	Het
Akap8	C	T	17: 32,530,036 (GRCm39)	R395H	possibly damaging	Het
Ankrd26	G	A	6: 118,517,420 (GRCm39)	A467V	possibly damaging	Het
Ano9	A	T	7: 140,682,227 (GRCm39)	S683T	probably benign	Het
Aox3	C	A	1: 58,164,222 (GRCm39)	A164E	probably benign	Het
Apc	T	C	18: 34,445,738 (GRCm39)	I878T	probably benign	Het
Apcdd1	C	T	18: 63,055,731 (GRCm39)		probably benign	Het
Atg9b	T	C	5: 24,593,107 (GRCm39)	N432S	probably benign	Het
Atp1b3	A	G	9: 96,227,911 (GRCm39)	F49L	probably damaging	Het
Cacnb2	A	T	2: 14,609,138 (GRCm39)		probably benign	Het
Ccdc180	T	A	4: 45,916,283 (GRCm39)	L828Q	probably null	Het
Chd3	C	T	11: 69,249,133 (GRCm39)	R748Q	probably benign	Het
Chst13	G	A	6: 90,286,506 (GRCm39)	P152L	probably damaging	Het
Cldn18	T	A	9: 99,581,028 (GRCm39)		probably null	Het
Cmklr1	C	T	5: 113,752,480 (GRCm39)	V174I	probably benign	Het
Cyp2d22	A	T	15: 82,256,688 (GRCm39)	V362D	probably damaging	Het
Dock10	T	C	1: 80,501,763 (GRCm39)	K1810R	probably damaging	Het
Duox1	T	A	2: 122,159,216 (GRCm39)	V661E	possibly damaging	Het
Ercc2	G	A	7: 19,125,899 (GRCm39)	R518Q	probably damaging	Het
Fam186a	A	G	15: 99,841,471 (GRCm39)	L1591P	probably damaging	Het
Fam186b	G	T	15: 99,178,419 (GRCm39)	D302E	probably benign	Het
Fap	T	C	2: 62,372,500 (GRCm39)	D326G	probably benign	Het
Gm42669	T	A	5: 107,655,892 (GRCm39)	D135E		Het
Keap1	A	T	9: 21,143,136 (GRCm39)	V463E	probably damaging	Het
Mga	T	C	2: 119,794,979 (GRCm39)	S2888P	probably damaging	Het
Mgam	A	G	6: 40,722,118 (GRCm39)	D403G	probably damaging	Het
Mios	C	T	6: 8,233,171 (GRCm39)	T773I	probably benign	Het
Nav3	T	C	10: 109,835,845 (GRCm39)	D79G	possibly damaging	Het
Or52d3	A	G	7: 104,228,855 (GRCm39)	M1V	probably null	Het
Or8k35	C	A	2: 86,424,771 (GRCm39)	V134F		Het
Orc6	T	C	8: 86,026,615 (GRCm39)	V20A	possibly damaging	Het
Peli2	A	G	14: 48,490,052 (GRCm39)	K175E	probably benign	Het
Plec	C	G	15: 76,062,924 (GRCm39)	R2382P	possibly damaging	Het
Ppp1r12b	T	A	1: 134,705,063 (GRCm39)	D875V	probably damaging	Het
Pramel6	T	A	2: 87,340,629 (GRCm39)	C320*	probably null	Het
Qrich2	A	T	11: 116,339,208 (GRCm39)	M26K	probably damaging	Het
Rapgef1	A	G	2: 29,624,291 (GRCm39)	I1006V	probably benign	Het
Rasgrp3	T	A	17: 75,821,158 (GRCm39)	L455Q	probably null	Het
Scaf8	C	T	17: 3,248,285 (GRCm39)	R1203W	probably damaging	Het
Senp6	C	T	9: 79,974,687 (GRCm39)		probably benign	Het
Senp7	A	G	16: 55,992,144 (GRCm39)	Y700C	probably damaging	Het
Siglec1	A	G	2: 130,915,246 (GRCm39)		probably null	Het
Slc12a7	G	A	13: 73,947,087 (GRCm39)	V592I	probably benign	Het
Slfn3	T	C	11: 83,103,825 (GRCm39)	V232A	probably benign	Het
Tdp1	T	C	12: 99,877,906 (GRCm39)	V396A	probably damaging	Het
Ticam1	T	C	17: 56,578,388 (GRCm39)	T236A	probably benign	Het
Tob1	AGCAGCAGCAGCAGCAGCAGCCGTCATCATCCCAGCCGCCGCCTCCACTACCGCAGCAGCAGCAGCAGCAGCC	AGCAGCAGCAGCAGCAGCAGCC	11: 94,105,205 (GRCm39)		probably benign	Het
Ttc21a	C	A	9: 119,787,181 (GRCm39)	D729E	probably damaging	Het
Ubtd2	T	C	11: 32,449,432 (GRCm39)	I93T	possibly damaging	Het
Unc45b	A	G	11: 82,808,586 (GRCm39)	D224G	probably benign	Het
Usp48	G	A	4: 137,340,996 (GRCm39)	G332E	probably benign	Het
Vmn2r61	A	G	7: 41,916,626 (GRCm39)	E413G	probably damaging	Het
Zfp423	A	T	8: 88,509,033 (GRCm39)	L437Q	probably damaging	Het
Zfp810	A	G	9: 22,190,227 (GRCm39)	V227A	possibly damaging	Het

Other mutations in Nxt2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R9016:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9018:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9064:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9065:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9066:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9114:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9115:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9144:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9145:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9146:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9147:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9148:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9149:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9326:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9327:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
R9480:Nxt2	UTSW	X	141,020,747 (GRCm39)	missense	possibly damaging	0.78
X0064:Nxt2	UTSW	X	141,012,590 (GRCm39)	start codon destroyed	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- GGGATGCCTGCTTAAAGTACAG -3'
(R):5'- ATCCAAACTGGCAGCTGATCTAG -3'

Sequencing Primer
(F):5'- GCCTGCTTAAAGTACAGAATAGTC -3'
(R):5'- TGGACCAATAGAGTGGGT -3'

Posted On

2022-07-18