Mutagenetix > Incidental Mutation

Incidental Mutation 'R9526:Atrnl1'

719291

Institutional Source

Beutler Lab

Gene Symbol

Atrnl1

Ensembl Gene

ENSMUSG00000054843

Gene Name

attractin like 1

Synonyms

Alp

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.187) question?

Stock #

R9526 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

57599466-58121775 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 57617551 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 109 (P109S)

Ref Sequence

ENSEMBL: ENSMUSP00000076514 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077282]

AlphaFold

Q6A051

Predicted Effect

probably damaging
Transcript: ENSMUST00000077282
AA Change: P109S

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000076514
Gene: ENSMUSG00000054843
AA Change: P109S

Domain	Start	End	E-Value	Type
low complexity region	25	32	N/A	INTRINSIC
EGF	61	90	5.71e-1	SMART
CUB	92	208	1.43e-11	SMART
EGF	209	244	1.95e1	SMART
Pfam:EGF_2	248	279	5.8e-7	PFAM
Pfam:Kelch_5	350	391	2.1e-9	PFAM
Pfam:Kelch_6	354	401	5.8e-8	PFAM
Pfam:Kelch_4	465	517	4.3e-7	PFAM
Pfam:Kelch_1	519	573	2.7e-6	PFAM
PSI	613	656	3.38e-1	SMART
PSI	665	708	2e-3	SMART
PSI	714	759	1.72e-2	SMART
CLECT	747	872	2.86e-20	SMART
PSI	888	938	6.26e-5	SMART
PSI	941	1011	1.73e-7	SMART
EGF_Lam	1013	1056	1.07e-5	SMART
low complexity region	1157	1173	N/A	INTRINSIC
transmembrane domain	1229	1251	N/A	INTRINSIC
low complexity region	1261	1272	N/A	INTRINSIC
low complexity region	1326	1339	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit normal coat coloring and normal brain morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn1	A	G	12: 80,230,393 (GRCm39)	L287S	probably damaging	Het
Adgrf1	A	T	17: 43,616,237 (GRCm39)	Q292L	possibly damaging	Het
Arhgap32	A	G	9: 32,172,026 (GRCm39)	D1602G	probably benign	Het
Camsap1	G	A	2: 25,843,962 (GRCm39)	H230Y	probably benign	Het
Capns1	C	T	7: 29,891,612 (GRCm39)	C144Y	probably damaging	Het
Ccdc17	G	A	4: 116,455,994 (GRCm39)	V341I	possibly damaging	Het
Cd36	T	C	5: 18,002,033 (GRCm39)	T323A	probably damaging	Het
Cdc14a	CGCTGCTGCTGCTGCTGCTG	CGCTGCTGCTGCTGCTG	3: 116,087,509 (GRCm39)		probably benign	Het
Ckap2l	G	A	2: 129,111,161 (GRCm39)	Q679*	probably null	Het
Clec4g	T	A	8: 3,768,565 (GRCm39)	N93I	probably benign	Het
Cntnap2	T	A	6: 45,992,165 (GRCm39)	L364Q	probably damaging	Het
Cul9	A	G	17: 46,841,026 (GRCm39)	M748T	probably benign	Het
Dnah11	A	T	12: 118,150,711 (GRCm39)	I349N	probably damaging	Het
Dnajc14	T	A	10: 128,642,260 (GRCm39)	Y61N	probably benign	Het
Dock6	A	T	9: 21,713,802 (GRCm39)	Y1909*	probably null	Het
Dock8	T	A	19: 25,165,739 (GRCm39)	D1874E	probably benign	Het
Dpm1	A	G	2: 168,072,210 (GRCm39)	S22P	probably benign	Het
Dysf	T	C	6: 84,128,885 (GRCm39)	L1385P	probably damaging	Het
Egf	T	C	3: 129,491,421 (GRCm39)	T859A	probably benign	Het
Eif2b1	A	T	5: 124,711,867 (GRCm39)	S162T	probably benign	Het
Emilin1	T	C	5: 31,075,484 (GRCm39)	L575P	probably damaging	Het
Erp27	T	A	6: 136,886,550 (GRCm39)	Q161L	probably benign	Het
Fbxw10	C	A	11: 62,765,945 (GRCm39)	D738E	possibly damaging	Het
Fcgbp	T	G	7: 27,790,937 (GRCm39)	C733G	probably damaging	Het
Fgb	T	A	3: 82,957,122 (GRCm39)		probably benign	Het
Gata3os	A	T	2: 9,887,634 (GRCm39)	T12S	unknown	Het
Got1l1	G	T	8: 27,688,503 (GRCm39)	Q283K	probably benign	Het
Grk2	G	A	19: 4,340,871 (GRCm39)	R226C	probably damaging	Het
Hoxa10	T	C	6: 52,211,334 (GRCm39)	D194G	probably benign	Het
Ipcef1	G	A	10: 6,840,620 (GRCm39)	T363I	probably damaging	Het
Itga10	C	A	3: 96,564,273 (GRCm39)	T922N	probably damaging	Het
Itgad	T	C	7: 127,777,552 (GRCm39)	I144T	probably benign	Het
Kat6b	C	A	14: 21,567,564 (GRCm39)	Q208K	possibly damaging	Het
Kidins220	T	G	12: 25,088,383 (GRCm39)	L1042R	probably damaging	Het
Kmt2c	T	C	5: 25,486,355 (GRCm39)	S4733G	probably damaging	Het
Lrig3	A	G	10: 125,850,736 (GRCm39)	I1101V	probably benign	Het
Lrp1	T	A	10: 127,431,229 (GRCm39)	N311I	probably damaging	Het
Man2a1	A	T	17: 64,958,310 (GRCm39)	K275I	probably benign	Het
Map3k10	T	A	7: 27,364,434 (GRCm39)	N318I	probably damaging	Het
Map3k8	G	A	18: 4,333,869 (GRCm39)	R408W	probably damaging	Het
Mast1	A	G	8: 85,647,805 (GRCm39)	M523T	probably damaging	Het
Mast4	T	C	13: 102,873,593 (GRCm39)	H1925R	probably benign	Het
Med12l	C	T	3: 58,984,207 (GRCm39)	S461L	probably damaging	Het
Mfap1a	T	C	2: 121,333,237 (GRCm39)	K65E	probably damaging	Het
Mical1	T	C	10: 41,358,602 (GRCm39)	S507P	probably benign	Het
Miga2	T	C	2: 30,268,400 (GRCm39)	V433A	unknown	Het
Mpdz	T	C	4: 81,274,653 (GRCm39)	T848A	probably benign	Het
Mycn	A	G	12: 12,989,778 (GRCm39)	V206A	probably benign	Het
Ndst1	G	A	18: 60,838,220 (GRCm39)	Q342*	probably null	Het
Nectin1	A	G	9: 43,702,369 (GRCm39)	M39V	probably benign	Het
Nectin4	A	T	1: 171,210,209 (GRCm39)	R234*	probably null	Het
Ngb	C	G	12: 87,145,317 (GRCm39)	V113L	possibly damaging	Het
Nphp3	T	A	9: 103,913,337 (GRCm39)	Y990N	probably damaging	Het
Nrdc	T	C	4: 108,915,833 (GRCm39)		probably null	Het
Or10a2	A	T	7: 106,673,739 (GRCm39)	K235*	probably null	Het
Or1e23	T	A	11: 73,407,351 (GRCm39)	I225F	probably damaging	Het
Or5p60	T	A	7: 107,723,801 (GRCm39)	Y223F	probably benign	Het
Osbpl6	A	G	2: 76,415,603 (GRCm39)	Y655C	probably damaging	Het
Pcdhgb4	A	G	18: 37,855,882 (GRCm39)	Q759R	probably benign	Het
Pcsk4	T	C	10: 80,161,800 (GRCm39)	D164G	probably damaging	Het
Pde4d	T	A	13: 110,071,915 (GRCm39)	I303N	probably damaging	Het
Pdf	T	A	8: 107,774,972 (GRCm39)	M87L	probably benign	Het
Pigb	A	G	9: 72,941,840 (GRCm39)	S140P	probably damaging	Het
Plch1	T	A	3: 63,758,549 (GRCm39)		probably benign	Het
Plxna1	G	T	6: 89,319,633 (GRCm39)	D557E	probably benign	Het
Polr2e	C	T	10: 79,872,792 (GRCm39)	V149M	probably benign	Het
Polr3a	A	T	14: 24,503,313 (GRCm39)	C1174S	probably benign	Het
Prb1c	T	A	6: 132,338,891 (GRCm39)	N109I	unknown	Het
Psmd2	T	A	16: 20,474,369 (GRCm39)	S308R	probably benign	Het
Ptger1	T	A	8: 84,396,002 (GRCm39)	V353E	probably damaging	Het
Ptprc	A	G	1: 137,996,111 (GRCm39)	M940T	probably benign	Het
Pus7l	T	C	15: 94,425,781 (GRCm39)	N540S	probably damaging	Het
Rap1gds1	T	A	3: 138,756,317 (GRCm39)	I13L	probably benign	Het
Rasl10b	A	T	11: 83,303,590 (GRCm39)	N49I	probably damaging	Het
Rftn1	A	T	17: 50,301,237 (GRCm39)	N537K	probably benign	Het
Rnase4	A	G	14: 51,342,645 (GRCm39)	Y123C	probably damaging	Het
Rnf181	A	C	6: 72,338,302 (GRCm39)	N26K	probably benign	Het
Rnf31	G	A	14: 55,836,269 (GRCm39)		probably null	Het
Rpusd3	C	G	6: 113,393,200 (GRCm39)	C304S	unknown	Het
Rsf1	CGGC	CGGCGGCGGGGGC	7: 97,229,139 (GRCm39)		probably benign	Het
Ryr3	C	A	2: 112,664,270 (GRCm39)	V1694L	probably benign	Het
Sanbr	T	C	11: 23,559,098 (GRCm39)	Y372C	probably damaging	Het
Sephs2	T	C	7: 126,872,346 (GRCm39)	D249G	probably damaging	Het
Serpina10	A	G	12: 103,583,217 (GRCm39)	I409T	probably damaging	Het
Serpina5	C	T	12: 104,069,403 (GRCm39)	A205V	probably damaging	Het
Sfr1	T	A	19: 47,723,453 (GRCm39)	V319E	probably damaging	Het
Skil	T	A	3: 31,171,639 (GRCm39)	Y542N	probably benign	Het
Slc15a5	T	C	6: 138,049,954 (GRCm39)	T154A	probably benign	Het
Slc27a2	T	A	2: 126,429,846 (GRCm39)	L618Q	probably damaging	Het
Slc6a6	A	T	6: 91,726,808 (GRCm39)	Y483F	probably benign	Het
Sprr1b	T	C	3: 92,344,443 (GRCm39)	I144M	probably benign	Het
Stat5a	T	A	11: 100,771,161 (GRCm39)	V580E		Het
Tnk1	C	T	11: 69,746,011 (GRCm39)	D305N	probably damaging	Het
Tox2	A	G	2: 163,164,930 (GRCm39)	*523W	probably null	Het
Trav12-2	A	G	14: 53,854,085 (GRCm39)	N20D	probably benign	Het
Trav16	T	A	14: 53,981,046 (GRCm39)	N78K	probably damaging	Het
Trf	C	T	9: 103,104,130 (GRCm39)	A78T	probably damaging	Het
Ttn	A	G	2: 76,711,543 (GRCm39)		probably benign	Het
Vmn2r67	T	A	7: 84,785,834 (GRCm39)	M724L	probably benign	Het
Vmn2r94	A	T	17: 18,477,261 (GRCm39)	F383L	probably benign	Het
Vwa2	T	C	19: 56,895,208 (GRCm39)	S461P	probably benign	Het
Zfp991	G	A	4: 147,264,327 (GRCm39)	G568E	probably damaging	Het
Zfpl1	A	G	19: 6,134,440 (GRCm39)	F15S	probably damaging	Het

Other mutations in Atrnl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Atrnl1	APN	19	57,680,249 (GRCm39)	missense	probably benign	0.02
IGL00707:Atrnl1	APN	19	57,661,697 (GRCm39)	missense	probably damaging	0.96
IGL00921:Atrnl1	APN	19	57,690,585 (GRCm39)	missense	probably damaging	1.00
IGL01410:Atrnl1	APN	19	58,119,536 (GRCm39)	missense	probably damaging	1.00
IGL01468:Atrnl1	APN	19	57,688,144 (GRCm39)	missense	probably benign	0.02
IGL01756:Atrnl1	APN	19	57,641,380 (GRCm39)	missense	probably benign
IGL01971:Atrnl1	APN	19	57,741,715 (GRCm39)	missense	probably damaging	1.00
IGL02019:Atrnl1	APN	19	57,680,195 (GRCm39)	splice site	probably benign
IGL02580:Atrnl1	APN	19	57,703,008 (GRCm39)	splice site	probably benign
IGL02649:Atrnl1	APN	19	57,638,873 (GRCm39)	splice site	probably benign
IGL02676:Atrnl1	APN	19	57,680,316 (GRCm39)	missense	probably damaging	1.00
IGL03276:Atrnl1	APN	19	57,641,359 (GRCm39)	missense	probably damaging	0.99
IGL03379:Atrnl1	APN	19	57,630,973 (GRCm39)	missense	probably benign	0.02
Magnetogorsk	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
polar	UTSW	19	57,641,382 (GRCm39)	missense	probably benign	0.00
PIT4812001:Atrnl1	UTSW	19	57,720,055 (GRCm39)	missense	probably benign	0.08
R0109:Atrnl1	UTSW	19	57,743,949 (GRCm39)	missense	possibly damaging	0.78
R0308:Atrnl1	UTSW	19	57,741,720 (GRCm39)	missense	probably benign	0.04
R0394:Atrnl1	UTSW	19	57,661,608 (GRCm39)	missense	probably benign	0.10
R0734:Atrnl1	UTSW	19	57,643,293 (GRCm39)	missense	probably damaging	1.00
R0811:Atrnl1	UTSW	19	57,661,573 (GRCm39)	missense	probably benign	0.07
R0812:Atrnl1	UTSW	19	57,661,573 (GRCm39)	missense	probably benign	0.07
R1183:Atrnl1	UTSW	19	57,638,725 (GRCm39)	missense	probably damaging	0.97
R1213:Atrnl1	UTSW	19	57,626,894 (GRCm39)	missense	probably benign	0.25
R1344:Atrnl1	UTSW	19	57,924,137 (GRCm39)	critical splice donor site	probably null
R1418:Atrnl1	UTSW	19	57,924,137 (GRCm39)	critical splice donor site	probably null
R1707:Atrnl1	UTSW	19	57,675,169 (GRCm39)	missense	probably benign	0.00
R1748:Atrnl1	UTSW	19	57,703,134 (GRCm39)	missense	probably damaging	0.99
R2051:Atrnl1	UTSW	19	57,680,281 (GRCm39)	missense	probably benign	0.01
R2113:Atrnl1	UTSW	19	57,744,048 (GRCm39)	nonsense	probably null
R2130:Atrnl1	UTSW	19	57,643,426 (GRCm39)	missense	probably damaging	1.00
R3710:Atrnl1	UTSW	19	57,645,546 (GRCm39)	missense	probably damaging	1.00
R3916:Atrnl1	UTSW	19	57,924,084 (GRCm39)	missense	possibly damaging	0.82
R4524:Atrnl1	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
R4707:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4712:Atrnl1	UTSW	19	57,641,382 (GRCm39)	missense	probably benign	0.00
R4784:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4785:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4798:Atrnl1	UTSW	19	58,030,793 (GRCm39)	missense	probably benign
R5172:Atrnl1	UTSW	19	57,673,945 (GRCm39)	nonsense	probably null
R5226:Atrnl1	UTSW	19	57,638,767 (GRCm39)	missense	probably benign
R5289:Atrnl1	UTSW	19	57,645,514 (GRCm39)	missense	probably damaging	1.00
R5372:Atrnl1	UTSW	19	57,743,968 (GRCm39)	missense	probably benign
R5737:Atrnl1	UTSW	19	57,766,320 (GRCm39)	missense	possibly damaging	0.84
R5782:Atrnl1	UTSW	19	57,741,718 (GRCm39)	missense	possibly damaging	0.95
R5826:Atrnl1	UTSW	19	57,618,724 (GRCm39)	nonsense	probably null
R6169:Atrnl1	UTSW	19	57,630,895 (GRCm39)	missense	probably benign	0.00
R6242:Atrnl1	UTSW	19	57,630,910 (GRCm39)	missense	probably benign	0.02
R6342:Atrnl1	UTSW	19	57,626,942 (GRCm39)	missense	probably damaging	1.00
R6372:Atrnl1	UTSW	19	57,638,764 (GRCm39)	missense	probably benign	0.01
R6811:Atrnl1	UTSW	19	57,643,393 (GRCm39)	missense	probably damaging	0.98
R6897:Atrnl1	UTSW	19	58,030,800 (GRCm39)	missense	probably benign	0.01
R7024:Atrnl1	UTSW	19	57,626,882 (GRCm39)	critical splice acceptor site	probably null
R7085:Atrnl1	UTSW	19	57,680,289 (GRCm39)	missense	probably damaging	1.00
R7144:Atrnl1	UTSW	19	58,030,784 (GRCm39)	missense	probably damaging	1.00
R7259:Atrnl1	UTSW	19	57,924,038 (GRCm39)	nonsense	probably null
R7289:Atrnl1	UTSW	19	57,638,846 (GRCm39)	missense	probably benign	0.13
R7310:Atrnl1	UTSW	19	57,630,856 (GRCm39)	missense	possibly damaging	0.69
R7372:Atrnl1	UTSW	19	57,924,078 (GRCm39)	missense	possibly damaging	0.47
R7432:Atrnl1	UTSW	19	57,743,956 (GRCm39)	missense	probably damaging	1.00
R7478:Atrnl1	UTSW	19	57,684,744 (GRCm39)	missense	possibly damaging	0.89
R7556:Atrnl1	UTSW	19	57,643,278 (GRCm39)	missense	probably benign
R7567:Atrnl1	UTSW	19	57,687,955 (GRCm39)	missense	probably damaging	0.98
R7608:Atrnl1	UTSW	19	57,703,119 (GRCm39)	missense	probably damaging	1.00
R7632:Atrnl1	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
R7655:Atrnl1	UTSW	19	57,599,811 (GRCm39)	nonsense	probably null
R7656:Atrnl1	UTSW	19	57,599,811 (GRCm39)	nonsense	probably null
R7718:Atrnl1	UTSW	19	57,728,615 (GRCm39)	nonsense	probably null
R7721:Atrnl1	UTSW	19	57,684,763 (GRCm39)	missense	probably benign	0.00
R7726:Atrnl1	UTSW	19	57,690,504 (GRCm39)	missense	probably damaging	1.00
R7733:Atrnl1	UTSW	19	57,690,420 (GRCm39)	missense	probably benign	0.00
R7774:Atrnl1	UTSW	19	57,688,103 (GRCm39)	missense	probably damaging	1.00
R8010:Atrnl1	UTSW	19	57,670,878 (GRCm39)	missense	probably benign	0.14
R8119:Atrnl1	UTSW	19	57,630,895 (GRCm39)	missense	probably benign	0.00
R9242:Atrnl1	UTSW	19	57,645,660 (GRCm39)	missense	probably benign	0.07
R9265:Atrnl1	UTSW	19	57,766,359 (GRCm39)	missense	probably benign	0.11
R9272:Atrnl1	UTSW	19	57,643,420 (GRCm39)	missense	probably benign	0.00
R9480:Atrnl1	UTSW	19	57,690,420 (GRCm39)	missense	possibly damaging	0.61
R9672:Atrnl1	UTSW	19	57,618,695 (GRCm39)	missense	possibly damaging	0.87
R9673:Atrnl1	UTSW	19	57,599,786 (GRCm39)	start codon destroyed	probably null	0.04
RF021:Atrnl1	UTSW	19	57,630,905 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ACAGCCGTGCTTTATCCAATTTG -3'
(R):5'- GCATATGCTCCAATAATAAGCAAGG -3'

Sequencing Primer
(F):5'- CCGTGCTTTATCCAATTTGAGGAG -3'
(R):5'- TGCTCCAATAATAAGCAAGGAAATG -3'

Posted On

2022-07-18