Mutagenetix > Incidental Mutation

Incidental Mutation 'R9533:Zfp85'

719579

Institutional Source

Beutler Lab

Gene Symbol

Zfp85

Ensembl Gene

ENSMUSG00000058331

Gene Name

zinc finger protein 85

Synonyms

Zfp85-rs1, KRAB19, Zfp71

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R9533 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67895919-67903347 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 67897722 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 117 (S117P)

Ref Sequence

ENSEMBL: ENSMUSP00000121202 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091520] [ENSMUST00000144183]

AlphaFold

Q7TMC9

Predicted Effect

probably benign
Transcript: ENSMUST00000091520

SMART Domains

Protein: ENSMUSP00000089105
Gene: ENSMUSG00000058331

Domain	Start	End	E-Value	Type
KRAB	5	65	5.78e-29	SMART
ZnF_C2H2	81	103	1.95e-3	SMART
ZnF_C2H2	109	131	1.22e-4	SMART
ZnF_C2H2	137	159	6.32e-3	SMART
ZnF_C2H2	165	187	2.95e-3	SMART
ZnF_C2H2	193	215	3.89e-3	SMART
ZnF_C2H2	221	243	3.89e-3	SMART
ZnF_C2H2	249	271	1.1e-2	SMART
ZnF_C2H2	277	299	2.91e-2	SMART
ZnF_C2H2	305	327	2.49e-1	SMART
ZnF_C2H2	333	355	2.09e-3	SMART
ZnF_C2H2	361	383	4.17e-3	SMART
ZnF_C2H2	389	411	2.95e-3	SMART
ZnF_C2H2	417	439	1.3e-4	SMART
ZnF_C2H2	445	467	1.04e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000144183
AA Change: S117P

PolyPhen 2 Score 0.076 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000121202
Gene: ENSMUSG00000058331
AA Change: S117P

Domain	Start	End	E-Value	Type
KRAB	45	105	5.78e-29	SMART
ZnF_C2H2	121	143	1.95e-3	SMART
ZnF_C2H2	149	171	1.22e-4	SMART
ZnF_C2H2	177	199	6.32e-3	SMART
ZnF_C2H2	205	227	2.95e-3	SMART
ZnF_C2H2	233	255	3.89e-3	SMART
ZnF_C2H2	261	283	3.89e-3	SMART
ZnF_C2H2	289	311	1.1e-2	SMART
ZnF_C2H2	317	339	2.91e-2	SMART
ZnF_C2H2	345	367	2.49e-1	SMART
ZnF_C2H2	373	395	2.09e-3	SMART
ZnF_C2H2	401	423	4.17e-3	SMART
ZnF_C2H2	429	451	2.95e-3	SMART
ZnF_C2H2	457	479	1.3e-4	SMART
ZnF_C2H2	485	507	1.04e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (40/40)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	C	T	11: 110,102,582 (GRCm39)	V881I	probably benign	Het
Acot1	C	T	12: 84,063,988 (GRCm39)	S57F		Het
Actr3	T	C	1: 125,339,048 (GRCm39)	I97V	probably benign	Het
C4b	A	T	17: 34,956,698 (GRCm39)	C633*	probably null	Het
Cdh19	T	A	1: 110,817,589 (GRCm39)	T718S	probably damaging	Het
Coasy	T	A	11: 100,975,852 (GRCm39)	S355T	probably benign	Het
Coch	A	G	12: 51,650,132 (GRCm39)	I374V	possibly damaging	Het
Dhrs7l	C	T	12: 72,675,050 (GRCm39)		probably benign	Het
Dip2b	T	A	15: 100,073,178 (GRCm39)	M760K	probably benign	Het
Evi5	A	G	5: 107,957,566 (GRCm39)	Y428H	probably damaging	Het
Fst	G	A	13: 114,592,397 (GRCm39)	R107*	probably null	Het
Gba1	A	G	3: 89,114,756 (GRCm39)	T387A	probably benign	Het
Gid8	A	T	2: 180,358,713 (GRCm39)	Q126L	probably damaging	Het
Iqgap1	A	G	7: 80,383,929 (GRCm39)	V1045A	possibly damaging	Het
Kif23	G	A	9: 61,832,924 (GRCm39)	S535L	probably benign	Het
Krt79	G	A	15: 101,848,417 (GRCm39)	A78V	possibly damaging	Het
Lama2	A	G	10: 26,862,871 (GRCm39)	F2964S	probably damaging	Het
Lamp3	T	C	16: 19,519,808 (GRCm39)	D125G	probably benign	Het
Lrrc15	C	T	16: 30,092,637 (GRCm39)	G234D	possibly damaging	Het
Myo7b	A	G	18: 32,108,297 (GRCm39)	V1188A	probably benign	Het
Nbeal2	T	C	9: 110,473,729 (GRCm39)	T37A	probably benign	Het
Nlrp1b	T	C	11: 71,109,095 (GRCm39)	I135M	probably benign	Het
Or2a51	A	G	6: 43,178,991 (GRCm39)	R138G	probably benign	Het
Or6c69	T	A	10: 129,747,404 (GRCm39)	I248F	probably benign	Het
P3h2	T	C	16: 25,789,725 (GRCm39)	Y514C	probably damaging	Het
Pcdha5	A	G	18: 37,093,986 (GRCm39)	N165S	probably damaging	Het
Phf10	G	C	17: 15,175,366 (GRCm39)	H157D	probably damaging	Het
Ppip5k2	C	T	1: 97,661,792 (GRCm39)	R708H	probably benign	Het
Prr12	A	G	7: 44,698,692 (GRCm39)	S408P	unknown	Het
Rab3a	A	T	8: 71,209,804 (GRCm39)	Y123F	probably damaging	Het
Rbak	A	T	5: 143,160,172 (GRCm39)	S294T	probably damaging	Het
Sarm1	T	C	11: 78,373,996 (GRCm39)	D677G	probably damaging	Het
Scly	A	T	1: 91,228,413 (GRCm39)		probably benign	Het
Slc26a6	T	C	9: 108,735,481 (GRCm39)	V385A	probably damaging	Het
Slc66a2	T	C	18: 80,327,034 (GRCm39)	V209A	unknown	Het
Srp72	T	C	5: 77,128,274 (GRCm39)	V165A	probably benign	Het
Tars3	A	C	7: 65,333,808 (GRCm39)		probably null	Het
Tspan1	T	A	4: 116,020,211 (GRCm39)	M233L	probably benign	Het
Zfp574	G	A	7: 24,780,379 (GRCm39)	G467D	probably damaging	Het
Zfp932	C	G	5: 110,157,787 (GRCm39)	P495R	probably damaging	Het

Other mutations in Zfp85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0383:Zfp85	UTSW	13	67,896,791 (GRCm39)	missense	probably benign	0.05
R1187:Zfp85	UTSW	13	67,897,835 (GRCm39)	missense	probably damaging	0.99
R1510:Zfp85	UTSW	13	67,903,084 (GRCm39)	intron	probably benign
R1775:Zfp85	UTSW	13	67,897,823 (GRCm39)	missense	probably damaging	0.99
R1803:Zfp85	UTSW	13	67,899,747 (GRCm39)	missense	probably benign	0.15
R2108:Zfp85	UTSW	13	67,897,003 (GRCm39)	missense	probably benign	0.08
R2412:Zfp85	UTSW	13	67,897,765 (GRCm39)	missense	probably damaging	0.99
R3147:Zfp85	UTSW	13	67,900,612 (GRCm39)	missense	probably damaging	0.96
R4811:Zfp85	UTSW	13	67,897,745 (GRCm39)	missense	probably damaging	0.97
R5308:Zfp85	UTSW	13	67,896,974 (GRCm39)	missense	probably damaging	1.00
R5334:Zfp85	UTSW	13	67,899,803 (GRCm39)	missense	probably damaging	0.99
R5373:Zfp85	UTSW	13	67,897,577 (GRCm39)	missense	probably damaging	1.00
R7045:Zfp85	UTSW	13	67,897,712 (GRCm39)	missense	probably benign	0.00
R7391:Zfp85	UTSW	13	67,897,410 (GRCm39)	missense	probably damaging	1.00
R7438:Zfp85	UTSW	13	67,897,064 (GRCm39)	missense	probably benign	0.09
R7544:Zfp85	UTSW	13	67,897,184 (GRCm39)	missense	probably benign	0.02
R8046:Zfp85	UTSW	13	67,897,098 (GRCm39)	nonsense	probably null
R8112:Zfp85	UTSW	13	67,896,893 (GRCm39)	missense	possibly damaging	0.71
R9615:Zfp85	UTSW	13	67,897,326 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TTGTAGGGTTTCTCTCCAGTATG -3'
(R):5'- GGCAGAGACATGCGGATTT -3'

Sequencing Primer
(F):5'- CTCTTCGGTGAACAATAAGTCCTG -3'
(R):5'- GGAAGGCCTTTGATAGGA -3'

Posted On

2022-07-18