Mutagenetix > Incidental Mutation

Incidental Mutation 'R9533:Lrrc15'

719585

Institutional Source

Beutler Lab

Gene Symbol

Lrrc15

Ensembl Gene

ENSMUSG00000052316

Gene Name

leucine rich repeat containing 15

Synonyms

5430427N11Rik

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9533 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

30088120-30102072 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 30092637 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 234 (G234D)

Ref Sequence

ENSEMBL: ENSMUSP00000066777 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064606]

AlphaFold

Q80X72

Predicted Effect

possibly damaging
Transcript: ENSMUST00000064606
AA Change: G234D

PolyPhen 2 Score 0.667 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000066777
Gene: ENSMUSG00000052316
AA Change: G234D

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
LRRNT	24	57	2.98e0	SMART
LRR	76	99	6.97e1	SMART
LRR_TYP	100	123	1.98e-4	SMART
LRR	124	147	4.08e0	SMART
LRR_TYP	148	171	2.4e-3	SMART
LRR_TYP	172	195	1.82e-3	SMART
LRR_TYP	196	219	1.18e-2	SMART
LRR_TYP	220	243	3.16e-3	SMART
LRR_TYP	245	267	3.39e-3	SMART
LRR_TYP	268	291	8.81e-2	SMART
LRR_TYP	292	315	9.5e-7	SMART
LRR_TYP	316	339	6.52e-5	SMART
LRR_TYP	340	363	7.78e-3	SMART
LRR_TYP	364	387	1.03e-2	SMART
LRR	388	411	8.48e0	SMART
LRRCT	423	474	1.11e-3	SMART
low complexity region	485	512	N/A	INTRINSIC
transmembrane domain	537	559	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (40/40)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	C	T	11: 110,102,582 (GRCm39)	V881I	probably benign	Het
Acot1	C	T	12: 84,063,988 (GRCm39)	S57F		Het
Actr3	T	C	1: 125,339,048 (GRCm39)	I97V	probably benign	Het
C4b	A	T	17: 34,956,698 (GRCm39)	C633*	probably null	Het
Cdh19	T	A	1: 110,817,589 (GRCm39)	T718S	probably damaging	Het
Coasy	T	A	11: 100,975,852 (GRCm39)	S355T	probably benign	Het
Coch	A	G	12: 51,650,132 (GRCm39)	I374V	possibly damaging	Het
Dhrs7l	C	T	12: 72,675,050 (GRCm39)		probably benign	Het
Dip2b	T	A	15: 100,073,178 (GRCm39)	M760K	probably benign	Het
Evi5	A	G	5: 107,957,566 (GRCm39)	Y428H	probably damaging	Het
Fst	G	A	13: 114,592,397 (GRCm39)	R107*	probably null	Het
Gba1	A	G	3: 89,114,756 (GRCm39)	T387A	probably benign	Het
Gid8	A	T	2: 180,358,713 (GRCm39)	Q126L	probably damaging	Het
Iqgap1	A	G	7: 80,383,929 (GRCm39)	V1045A	possibly damaging	Het
Kif23	G	A	9: 61,832,924 (GRCm39)	S535L	probably benign	Het
Krt79	G	A	15: 101,848,417 (GRCm39)	A78V	possibly damaging	Het
Lama2	A	G	10: 26,862,871 (GRCm39)	F2964S	probably damaging	Het
Lamp3	T	C	16: 19,519,808 (GRCm39)	D125G	probably benign	Het
Myo7b	A	G	18: 32,108,297 (GRCm39)	V1188A	probably benign	Het
Nbeal2	T	C	9: 110,473,729 (GRCm39)	T37A	probably benign	Het
Nlrp1b	T	C	11: 71,109,095 (GRCm39)	I135M	probably benign	Het
Or2a51	A	G	6: 43,178,991 (GRCm39)	R138G	probably benign	Het
Or6c69	T	A	10: 129,747,404 (GRCm39)	I248F	probably benign	Het
P3h2	T	C	16: 25,789,725 (GRCm39)	Y514C	probably damaging	Het
Pcdha5	A	G	18: 37,093,986 (GRCm39)	N165S	probably damaging	Het
Phf10	G	C	17: 15,175,366 (GRCm39)	H157D	probably damaging	Het
Ppip5k2	C	T	1: 97,661,792 (GRCm39)	R708H	probably benign	Het
Prr12	A	G	7: 44,698,692 (GRCm39)	S408P	unknown	Het
Rab3a	A	T	8: 71,209,804 (GRCm39)	Y123F	probably damaging	Het
Rbak	A	T	5: 143,160,172 (GRCm39)	S294T	probably damaging	Het
Sarm1	T	C	11: 78,373,996 (GRCm39)	D677G	probably damaging	Het
Scly	A	T	1: 91,228,413 (GRCm39)		probably benign	Het
Slc26a6	T	C	9: 108,735,481 (GRCm39)	V385A	probably damaging	Het
Slc66a2	T	C	18: 80,327,034 (GRCm39)	V209A	unknown	Het
Srp72	T	C	5: 77,128,274 (GRCm39)	V165A	probably benign	Het
Tars3	A	C	7: 65,333,808 (GRCm39)		probably null	Het
Tspan1	T	A	4: 116,020,211 (GRCm39)	M233L	probably benign	Het
Zfp574	G	A	7: 24,780,379 (GRCm39)	G467D	probably damaging	Het
Zfp85	A	G	13: 67,897,722 (GRCm39)	S117P	probably benign	Het
Zfp932	C	G	5: 110,157,787 (GRCm39)	P495R	probably damaging	Het

Other mutations in Lrrc15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00495:Lrrc15	APN	16	30,092,848 (GRCm39)	missense	possibly damaging	0.90
IGL01720:Lrrc15	APN	16	30,092,138 (GRCm39)	missense	probably benign	0.19
R0138:Lrrc15	UTSW	16	30,092,267 (GRCm39)	missense	possibly damaging	0.63
R0317:Lrrc15	UTSW	16	30,092,561 (GRCm39)	missense	probably benign
R0497:Lrrc15	UTSW	16	30,091,710 (GRCm39)	missense	probably damaging	0.98
R0528:Lrrc15	UTSW	16	30,092,566 (GRCm39)	missense	probably damaging	0.99
R1122:Lrrc15	UTSW	16	30,092,719 (GRCm39)	missense	probably damaging	1.00
R1950:Lrrc15	UTSW	16	30,092,649 (GRCm39)	missense	probably benign	0.21
R2898:Lrrc15	UTSW	16	30,092,604 (GRCm39)	missense	probably benign	0.01
R4272:Lrrc15	UTSW	16	30,092,673 (GRCm39)	missense	probably benign	0.06
R4839:Lrrc15	UTSW	16	30,093,086 (GRCm39)	missense	probably benign	0.00
R5091:Lrrc15	UTSW	16	30,092,172 (GRCm39)	missense	probably damaging	1.00
R5369:Lrrc15	UTSW	16	30,091,722 (GRCm39)	missense	possibly damaging	0.93
R6076:Lrrc15	UTSW	16	30,091,806 (GRCm39)	missense	probably benign	0.25
R6133:Lrrc15	UTSW	16	30,093,054 (GRCm39)	missense	probably benign	0.11
R7017:Lrrc15	UTSW	16	30,091,780 (GRCm39)	missense	probably benign	0.00
R9217:Lrrc15	UTSW	16	30,092,415 (GRCm39)	missense	probably damaging	1.00
R9507:Lrrc15	UTSW	16	30,092,829 (GRCm39)	missense	probably damaging	1.00
R9562:Lrrc15	UTSW	16	30,093,016 (GRCm39)	missense	probably damaging	1.00
R9565:Lrrc15	UTSW	16	30,093,016 (GRCm39)	missense	probably damaging	1.00
R9616:Lrrc15	UTSW	16	30,092,517 (GRCm39)	missense	probably damaging	1.00
R9641:Lrrc15	UTSW	16	30,093,006 (GRCm39)	missense	probably damaging	1.00
R9755:Lrrc15	UTSW	16	30,093,147 (GRCm39)	missense	possibly damaging	0.95
Z1176:Lrrc15	UTSW	16	30,093,070 (GRCm39)	missense	probably benign	0.31

Predicted Primers

PCR Primer
(F):5'- TGTGATTGTTGTAGAGCCAAAGC -3'
(R):5'- CTGGTAGGTCTCACCAAACTC -3'

Sequencing Primer
(F):5'- AAAGCTCCCTCAGGTTGGG -3'
(R):5'- GGTAGGTCTCACCAAACTCAACCTG -3'

Posted On

2022-07-18