Mutagenetix > Incidental Mutation

Incidental Mutation 'R9537:Zfy2'

719761

Institutional Source

Beutler Lab

Gene Symbol

Zfy2

Ensembl Gene

ENSMUSG00000000103

Gene Name

zinc finger protein 2, Y-linked

Synonyms

Zfy-2

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R9537 (G1)

Quality Score

222.006

Status

Not validated

Chromosome

Chromosomal Location

2106015-2170409 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 2108596 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 355 (T355S)

Ref Sequence

ENSEMBL: ENSMUSP00000111557 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000115891] [ENSMUST00000187148]

AlphaFold

P20662

Predicted Effect

SMART Domains

Protein: ENSMUSP00000111557
Gene: ENSMUSG00000000103
AA Change: T355S

Domain	Start	End	E-Value	Type
low complexity region	3	13	N/A	INTRINSIC
Pfam:Zfx_Zfy_act	68	388	1.1e-115	PFAM
ZnF_C2H2	403	425	3.69e-4	SMART
ZnF_C2H2	434	456	1.26e-2	SMART
ZnF_C2H2	491	514	1.41e0	SMART
ZnF_C2H2	520	542	3.69e-4	SMART
ZnF_C2H2	548	571	3.63e-3	SMART
ZnF_C2H2	577	599	1.02e1	SMART
ZnF_C2H2	605	628	3.58e-2	SMART
ZnF_C2H2	634	656	2.95e-3	SMART
ZnF_C2H2	662	685	1.23e0	SMART
ZnF_C2H2	691	713	1.45e-2	SMART
ZnF_C2H2	719	742	1.2e-3	SMART
ZnF_C2H2	748	770	3.34e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000187148
AA Change: T355S

PolyPhen 2 Score 0.047 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000139591
Gene: ENSMUSG00000000103
AA Change: T355S

Domain	Start	End	E-Value	Type
low complexity region	3	13	N/A	INTRINSIC
Pfam:Zfx_Zfy_act	67	388	2.6e-149	PFAM
ZnF_C2H2	403	425	3.69e-4	SMART
ZnF_C2H2	434	456	1.26e-2	SMART
ZnF_C2H2	491	514	1.41e0	SMART
ZnF_C2H2	520	542	3.69e-4	SMART
ZnF_C2H2	548	571	3.63e-3	SMART
ZnF_C2H2	577	599	1.02e1	SMART
ZnF_C2H2	605	628	3.58e-2	SMART
ZnF_C2H2	634	656	2.95e-3	SMART
ZnF_C2H2	662	685	1.23e0	SMART
ZnF_C2H2	691	713	1.45e-2	SMART
ZnF_C2H2	719	742	1.2e-3	SMART
ZnF_C2H2	748	770	3.34e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310007B03Rik	T	C	1: 93,153,162	K341E	possibly damaging	Het
Adgra3	A	G	5: 49,960,865	Y1114H	possibly damaging	Het
Atp5j	A	G	16: 84,828,470	Y82H	probably damaging	Het
Bcl2a1d	T	G	9: 88,731,473	I83L	probably benign	Het
Bean1	CT	C	8: 104,182,032		probably null	Het
Bglap3	T	C	3: 88,368,832	D71G	probably benign	Het
Bnip3l	G	A	14: 67,008,765	P7L	possibly damaging	Het
Chmp2b	T	C	16: 65,551,046	K15E	probably benign	Het
Chrm3	A	T	13: 9,877,426	W525R	probably damaging	Het
Col7a1	A	T	9: 108,955,352	K143*	probably null	Het
D6Ertd527e	G	C	6: 87,111,857	S334T	unknown	Het
Dnaic1	A	G	4: 41,629,790		probably null	Het
Dnhd1	G	A	7: 105,695,533	G2028D	probably damaging	Het
Esyt3	C	T	9: 99,317,239	R773Q	probably damaging	Het
Ffar1	T	C	7: 30,860,600	T291A	probably benign	Het
Gm5767	G	T	16: 8,683,313	C11F		Het
Golga2	G	T	2: 32,288,301		probably benign	Het
Hapln2	T	A	3: 88,024,473		probably null	Het
Ing1	T	C	8: 11,561,889	L203P	probably benign	Het
Med1	T	C	11: 98,171,760	T171A	possibly damaging	Het
Mib2	A	T	4: 155,657,495	L387H	probably damaging	Het
Mier1	A	G	4: 103,162,561	N494S	probably benign	Het
Myof	A	G	19: 37,907,606	L1847P	probably damaging	Het
Ndst3	A	T	3: 123,671,513	V270D		Het
Nup160	T	C	2: 90,729,744	L1271S	possibly damaging	Het
Osgep	A	T	14: 50,924,662		probably null	Het
Ptgfr	G	T	3: 151,835,808	T21N	possibly damaging	Het
Ptgs1	T	C	2: 36,230,727	S23P	unknown	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,579,914		probably benign	Het
Smu1	A	G	4: 40,755,671	S65P	probably benign	Het
Spef2	T	C	15: 9,601,799	Y1459C	unknown	Het
Spen	A	G	4: 141,471,704	V3204A	probably benign	Het
Spen	TTGCTGCTGCTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTGCTGCTGCTG	4: 141,516,845		probably benign	Het
Timm44	G	A	8: 4,260,576	T392I	possibly damaging	Het
Tnc	A	T	4: 63,966,584	I1818N	probably damaging	Het
Trpm1	G	T	7: 64,153,868		probably benign	Het
Ttc14	A	G	3: 33,803,198	Y231C	probably damaging	Het
Ube3b	A	G	5: 114,387,184	R23G	probably damaging	Het
Usp40	T	C	1: 88,007,395	Y10C	probably benign	Het
Vmn2r107	C	A	17: 20,374,887	S567R	probably benign	Het
Zfp112	T	C	7: 24,127,087	Y831H	probably damaging	Het

Other mutations in Zfy2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02850:Zfy2	APN	Y	2106894	missense	probably benign	0.01
IGL02850:Zfy2	APN	Y	2117188	missense	probably benign	0.00
IGL02851:Zfy2	APN	Y	2106894	missense	probably benign	0.01
IGL02851:Zfy2	APN	Y	2117188	missense	probably benign	0.00
IGL02852:Zfy2	APN	Y	2106894	missense	probably benign	0.01
IGL02852:Zfy2	APN	Y	2117188	missense	probably benign	0.00
PIT4515001:Zfy2	UTSW	Y	2117096	missense	probably benign	0.09
R0426:Zfy2	UTSW	Y	2107348	missense	possibly damaging	0.61
R0490:Zfy2	UTSW	Y	2106620	missense	possibly damaging	0.89
R1080:Zfy2	UTSW	Y	2121645	missense	probably benign	0.00
R1513:Zfy2	UTSW	Y	2116185	missense	probably benign	0.00
R1935:Zfy2	UTSW	Y	2121496	missense	probably benign	0.02
R1936:Zfy2	UTSW	Y	2121496	missense	probably benign	0.02
R2358:Zfy2	UTSW	Y	2107272	missense	possibly damaging	0.61
R4484:Zfy2	UTSW	Y	2107351	missense	possibly damaging	0.86
R4754:Zfy2	UTSW	Y	2121477	missense	probably benign	0.02
R4777:Zfy2	UTSW	Y	2116194	missense	probably benign	0.00
R4812:Zfy2	UTSW	Y	2106334	missense	probably benign	0.08
R5045:Zfy2	UTSW	Y	2107159	missense	possibly damaging	0.77
R5363:Zfy2	UTSW	Y	2106555	missense	possibly damaging	0.95
R6256:Zfy2	UTSW	Y	2116267	missense	probably benign	0.02
R6618:Zfy2	UTSW	Y	2121477	missense	probably benign	0.10
R6941:Zfy2	UTSW	Y	2121491	missense	probably benign	0.02
R7011:Zfy2	UTSW	Y	2107127	missense	possibly damaging	0.59
R7712:Zfy2	UTSW	Y	2121420	missense	probably benign	0.05
R7759:Zfy2	UTSW	Y	2117083	missense	probably benign	0.02
R7985:Zfy2	UTSW	Y	2116263	missense	probably benign	0.00
R8051:Zfy2	UTSW	Y	2117380	intron	probably benign
R8218:Zfy2	UTSW	Y	2133421	missense	unknown
R8345:Zfy2	UTSW	Y	2107096	missense	possibly damaging	0.95
R8371:Zfy2	UTSW	Y	2117168	missense	probably benign	0.00
R8830:Zfy2	UTSW	Y	2106600	missense	possibly damaging	0.60
R9448:Zfy2	UTSW	Y	2109904	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GCACAAAAGCTACCAGGTGG -3'
(R):5'- GGCGAACTACCAACTAAATGTATTC -3'

Sequencing Primer
(F):5'- CCAGGTGGTGATTAGTCAAAAC -3'
(R):5'- CTTTTCACTGTTGTAGTACTCA -3'

Posted On

2022-07-18