Mutagenetix > Incidental Mutation

Incidental Mutation 'R9544:Or7g25'

720003

Institutional Source

Beutler Lab

Gene Symbol

Or7g25

Ensembl Gene

ENSMUSG00000048391

Gene Name

olfactory receptor family 7 subfamily G member 25

Synonyms

GA_x6K02T2PVTD-12986331-12985390, Olfr843, MOR155-2

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R9544 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19159653-19160759 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19160561 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 45 (I45F)

Ref Sequence

ENSEMBL: ENSMUSP00000150008 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061365] [ENSMUST00000214019] [ENSMUST00000214267]

AlphaFold

Q7TRG4

Predicted Effect

probably damaging
Transcript: ENSMUST00000061365
AA Change: I45F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000051027
Gene: ENSMUSG00000048391
AA Change: I45F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	2.4e-51	PFAM
Pfam:7tm_1	41	290	3.3e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000214019
AA Change: I45F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000214267
AA Change: I45F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 96.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5530400C23Rik	A	G	6: 133,271,202 (GRCm39)	E82G	possibly damaging	Het
Adra2a	C	G	19: 54,035,454 (GRCm39)	P270R	probably benign	Het
Alox12b	A	T	11: 69,054,812 (GRCm39)	I272F	possibly damaging	Het
Als2	C	A	1: 59,250,468 (GRCm39)	E425D	probably benign	Het
Arhgap21	C	A	2: 20,858,938 (GRCm39)	C1252F	possibly damaging	Het
Arhgap31	T	G	16: 38,423,976 (GRCm39)	T697P	probably damaging	Het
Aspm	C	A	1: 139,385,523 (GRCm39)	T389K	probably benign	Het
B4galnt3	T	A	6: 120,209,905 (GRCm39)	I73F	probably damaging	Het
Btn2a2	A	G	13: 23,672,008 (GRCm39)	V27A	probably benign	Het
Cep97	A	T	16: 55,735,303 (GRCm39)	N570K	possibly damaging	Het
Cfh	T	C	1: 140,036,266 (GRCm39)	I751V	probably benign	Het
Chd3	A	G	11: 69,241,046 (GRCm39)	C1516R	probably damaging	Het
Cul3	A	G	1: 80,258,576 (GRCm39)	F487L	probably damaging	Het
Dock1	T	C	7: 134,348,186 (GRCm39)	S266P	possibly damaging	Het
Dock6	A	T	9: 21,732,830 (GRCm39)	Y1118*	probably null	Het
Dolpp1	T	A	2: 30,282,515 (GRCm39)	Y23N	probably damaging	Het
Egf	T	C	3: 129,511,617 (GRCm39)	M517V	probably benign	Het
Endou	C	A	15: 97,609,969 (GRCm39)	K442N	probably damaging	Het
Fam210b	C	T	2: 172,194,633 (GRCm39)	R162W	probably damaging	Het
Fbxw27	ATGT	AT	9: 109,602,322 (GRCm39)		probably null	Het
Flt3	T	A	5: 147,291,442 (GRCm39)	Q641L	possibly damaging	Het
Ighv3-3	T	A	12: 114,160,270 (GRCm39)	I47F	probably damaging	Het
Il4	C	T	11: 53,504,837 (GRCm39)	R76H	probably damaging	Het
Inpp5f	A	C	7: 128,278,515 (GRCm39)	D435A	possibly damaging	Het
Kcnq2	T	G	2: 180,729,407 (GRCm39)	D532A	probably damaging	Het
Krtap11-1	A	C	16: 89,368,062 (GRCm39)		probably benign	Het
Lamp3	T	A	16: 19,494,832 (GRCm39)		probably null	Het
Lcn3	A	T	2: 25,656,151 (GRCm39)	I69L	probably benign	Het
Lrrc43	A	T	5: 123,641,307 (GRCm39)	H497L	probably damaging	Het
Luc7l2	T	G	6: 38,580,315 (GRCm39)	S305A	unknown	Het
Malrd1	C	T	2: 15,640,809 (GRCm39)	T513I	unknown	Het
Mllt3	T	A	4: 87,759,418 (GRCm39)	D210V	probably damaging	Het
Mmp14	A	G	14: 54,673,251 (GRCm39)	H53R	possibly damaging	Het
Ndst4	A	C	3: 125,476,808 (GRCm39)	H11P	probably damaging	Het
Nln	G	A	13: 104,198,356 (GRCm39)	T152M	probably benign	Het
Oas1c	A	G	5: 120,946,202 (GRCm39)	F99L	probably benign	Het
Or10s1	T	C	9: 39,986,353 (GRCm39)	L254P	probably damaging	Het
Or1e35	T	C	11: 73,797,637 (GRCm39)	K227R	probably benign	Het
Or1j11	G	T	2: 36,311,784 (GRCm39)	A125S	probably damaging	Het
Or5w15	G	A	2: 87,568,644 (GRCm39)	T8I	probably benign	Het
Pappa2	C	A	1: 158,784,817 (GRCm39)	Q64H	probably damaging	Het
Pcdhb15	T	C	18: 37,608,948 (GRCm39)	F727L	probably benign	Het
Pcsk1	A	T	13: 75,259,039 (GRCm39)	N271I	probably damaging	Het
Pkhd1l1	G	A	15: 44,410,239 (GRCm39)	G2582D	probably damaging	Het
Psma4	A	T	9: 54,862,245 (GRCm39)	Q123L	probably damaging	Het
Selenbp2	A	T	3: 94,605,368 (GRCm39)	I127F	possibly damaging	Het
Shank1	G	A	7: 43,962,342 (GRCm39)	S71N	unknown	Het
Slc29a3	G	T	10: 60,551,960 (GRCm39)	C361*	probably null	Het
Slx4	C	A	16: 3,797,917 (GRCm39)	R1489L	probably damaging	Het
Sorl1	A	C	9: 41,993,105 (GRCm39)	Y306*	probably null	Het
Tbc1d16	G	T	11: 119,101,335 (GRCm39)	H58Q	probably damaging	Het
Tfdp2	A	G	9: 96,169,663 (GRCm39)	T5A	probably damaging	Het
Thbs1	G	T	2: 117,953,932 (GRCm39)	G1061V	probably damaging	Het
Timm29	G	A	9: 21,504,662 (GRCm39)	R110H	probably damaging	Het
Tmf1	C	T	6: 97,147,293 (GRCm39)	E558K	possibly damaging	Het
Trappc11	T	C	8: 47,972,713 (GRCm39)	T287A	possibly damaging	Het
Unc119b	G	A	5: 115,272,827 (GRCm39)	T48M	probably benign	Het
Vmn1r123	T	C	7: 20,896,987 (GRCm39)	I293T	probably benign	Het
Vmn2r67	G	A	7: 84,786,317 (GRCm39)	H563Y	possibly damaging	Het
Vps8	G	T	16: 21,336,893 (GRCm39)	V763L	probably benign	Het
Wdr3	A	T	3: 100,050,752 (GRCm39)	Y722*	probably null	Het
Xrn1	A	G	9: 95,920,756 (GRCm39)	T1339A	probably benign	Het
Zfp827	T	A	8: 79,905,604 (GRCm39)	H860Q	probably damaging	Het

Other mutations in Or7g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02104:Or7g25	APN	9	19,160,166 (GRCm39)	missense	probably benign	0.21
IGL02338:Or7g25	APN	9	19,159,960 (GRCm39)	missense	probably damaging	0.98
IGL03197:Or7g25	APN	9	19,160,098 (GRCm39)	missense	probably benign	0.37
IGL03227:Or7g25	APN	9	19,160,518 (GRCm39)	missense	probably damaging	1.00
IGL03046:Or7g25	UTSW	9	19,160,441 (GRCm39)	missense	probably damaging	0.99
IGL03052:Or7g25	UTSW	9	19,159,938 (GRCm39)	nonsense	probably null
R0423:Or7g25	UTSW	9	19,160,248 (GRCm39)	nonsense	probably null
R1316:Or7g25	UTSW	9	19,160,035 (GRCm39)	missense	probably benign	0.06
R1759:Or7g25	UTSW	9	19,160,384 (GRCm39)	missense	probably benign	0.36
R1782:Or7g25	UTSW	9	19,159,877 (GRCm39)	missense	probably benign	0.04
R2517:Or7g25	UTSW	9	19,160,357 (GRCm39)	missense	probably benign	0.21
R3196:Or7g25	UTSW	9	19,160,495 (GRCm39)	missense	probably damaging	1.00
R3742:Or7g25	UTSW	9	19,159,979 (GRCm39)	missense	possibly damaging	0.91
R4191:Or7g25	UTSW	9	19,160,383 (GRCm39)	missense	probably benign	0.05
R4320:Or7g25	UTSW	9	19,160,052 (GRCm39)	missense	probably damaging	1.00
R4523:Or7g25	UTSW	9	19,160,525 (GRCm39)	missense	probably damaging	0.98
R4756:Or7g25	UTSW	9	19,160,153 (GRCm39)	missense	possibly damaging	0.56
R4908:Or7g25	UTSW	9	19,160,149 (GRCm39)	missense	probably benign	0.00
R5319:Or7g25	UTSW	9	19,160,329 (GRCm39)	missense	possibly damaging	0.60
R5554:Or7g25	UTSW	9	19,160,039 (GRCm39)	missense	probably benign	0.02
R6011:Or7g25	UTSW	9	19,159,807 (GRCm39)	missense	probably damaging	0.98
R7330:Or7g25	UTSW	9	19,160,567 (GRCm39)	missense	probably benign	0.01
R7862:Or7g25	UTSW	9	19,160,736 (GRCm39)	start gained	probably benign

Predicted Primers

PCR Primer
(F):5'- TCACAGCAAGTAGACAACTTTCTAAGC -3'
(R):5'- ATGTGACCCCAAAGAATTTGTG -3'

Sequencing Primer
(F):5'- GACAACTTTCTAAGCCACCAAAAATC -3'
(R):5'- GTGACCCCAAAGAATTTGTGAAAAC -3'

Posted On

2022-07-18