Mutagenetix > Incidental Mutation

Incidental Mutation 'R9547:Or5k3'

720210

Institutional Source

Beutler Lab

Gene Symbol

Or5k3

Ensembl Gene

ENSMUSG00000062608

Gene Name

olfactory receptor family 5 subfamily K member 3

Synonyms

MOR184-5, GA_x54KRFPKG5P-55369823-55370749, Olfr195

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.085) question?

Stock #

R9547 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

58969215-58970141 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 58970107 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 298 (I298T)

Ref Sequence

ENSEMBL: ENSMUSP00000150249 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075381] [ENSMUST00000216957]

AlphaFold

Q8VGQ6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000075381
AA Change: I298T

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000073737
Gene: ENSMUSG00000062608
AA Change: I298T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	4.1e-53	PFAM
Pfam:7TM_GPCR_Srsx	35	161	2.7e-8	PFAM
Pfam:7tm_1	41	307	9.4e-17	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000216957
AA Change: I298T

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2fm3	G	T	3: 59,772,656 (GRCm39)	M53I	probably benign	Het
Abtb1	C	T	6: 88,815,917 (GRCm39)	V185M	probably damaging	Het
Acod1	A	T	14: 103,292,294 (GRCm39)	S273C	probably benign	Het
Aifm3	T	C	16: 17,317,604 (GRCm39)	V75A	probably benign	Het
Anks1	T	C	17: 28,270,748 (GRCm39)	L845P	probably damaging	Het
Apc	A	G	18: 34,445,311 (GRCm39)	K736E	possibly damaging	Het
Atad2	A	G	15: 57,989,973 (GRCm39)	S168P	probably damaging	Het
Cand2	G	A	6: 115,759,757 (GRCm39)	A143T	probably benign	Het
Cep290	A	T	10: 100,380,841 (GRCm39)	H116L	probably benign	Het
Chd9	G	A	8: 91,683,186 (GRCm39)	R542H	unknown	Het
Crnkl1	T	C	2: 145,772,550 (GRCm39)	M176V	possibly damaging	Het
Dnah14	A	C	1: 181,420,992 (GRCm39)		probably null	Het
Dync1h1	A	G	12: 110,624,805 (GRCm39)	E3744G	probably damaging	Het
Ednrb	T	C	14: 104,080,459 (GRCm39)	I152V	probably benign	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
Fat3	A	T	9: 15,911,142 (GRCm39)	I1620N	possibly damaging	Het
Fbxo4	G	A	15: 3,998,493 (GRCm39)	P322S	probably damaging	Het
Fbxw10	G	T	11: 62,767,647 (GRCm39)	V828F	possibly damaging	Het
Gm7579	CTGTGTG	CTGTG	7: 141,765,736 (GRCm39)		probably null	Het
Gmip	T	C	8: 70,273,381 (GRCm39)	S891P	possibly damaging	Het
Grb10	A	G	11: 11,893,919 (GRCm39)	I389T	probably benign	Het
Grip1	G	A	10: 119,874,569 (GRCm39)	E778K	possibly damaging	Het
Hhatl	A	G	9: 121,618,649 (GRCm39)	Y118H	probably damaging	Het
Ighv1-69	A	G	12: 115,586,885 (GRCm39)	F83L	possibly damaging	Het
Intu	G	A	3: 40,608,536 (GRCm39)	V183I	probably benign	Het
Ipo4	A	G	14: 55,870,789 (GRCm39)		probably null	Het
Kdm4c	A	C	4: 74,323,104 (GRCm39)	D1012A	possibly damaging	Het
Klrc2	C	G	6: 129,633,812 (GRCm39)	A188P	probably benign	Het
Lipc	T	C	9: 70,728,146 (GRCm39)	D104G	unknown	Het
Lrrc63	A	T	14: 75,344,828 (GRCm39)	L420M	probably damaging	Het
Ly75	C	A	2: 60,161,069 (GRCm39)		probably null	Het
Mrpl50	T	A	4: 49,514,338 (GRCm39)	H111L	probably damaging	Het
Mtmr9	A	G	14: 63,779,855 (GRCm39)	I78T	possibly damaging	Het
Npepl1	A	G	2: 173,962,030 (GRCm39)	D304G	probably null	Het
Nphs1	C	T	7: 30,180,875 (GRCm39)	S1093F	probably benign	Het
Or2n1d	G	T	17: 38,646,341 (GRCm39)	V98F	possibly damaging	Het
Or8g24	G	A	9: 38,989,927 (GRCm39)	T38I	probably damaging	Het
Pcdh18	A	T	3: 49,709,506 (GRCm39)	I603K	possibly damaging	Het
Pdcd6ip	A	T	9: 113,484,174 (GRCm39)	Y818N	unknown	Het
Phf10	A	G	17: 15,166,459 (GRCm39)		probably null	Het
Phgdh	A	G	3: 98,241,950 (GRCm39)	S55P	probably damaging	Het
Poll	G	T	19: 45,546,359 (GRCm39)	P227H	probably benign	Het
Prdm2	A	T	4: 142,861,561 (GRCm39)	S576R	probably damaging	Het
Psg26	T	C	7: 18,214,087 (GRCm39)	I192V	probably benign	Het
Rpgrip1l	T	C	8: 91,977,873 (GRCm39)	D1038G	probably benign	Het
Snx18	A	G	13: 113,753,754 (GRCm39)	M393T	possibly damaging	Het
Srsf11	A	G	3: 157,717,735 (GRCm39)	C448R	unknown	Het
Sv2c	A	G	13: 96,185,008 (GRCm39)	I223T	probably benign	Het
Tbc1d1	T	C	5: 64,330,950 (GRCm39)	V43A	possibly damaging	Het
Tex21	T	C	12: 76,253,591 (GRCm39)	T441A	probably damaging	Het
Thnsl2	T	A	6: 71,116,810 (GRCm39)	D114V	probably damaging	Het
Trpm2	A	G	10: 77,748,467 (GRCm39)	V1401A	probably benign	Het
Ttll6	T	C	11: 96,049,588 (GRCm39)	S769P	probably benign	Het
Tut4	A	G	4: 108,370,429 (GRCm39)	D776G	probably benign	Het
Uba5	A	G	9: 103,931,567 (GRCm39)	S222P	probably damaging	Het
Ublcp1	T	C	11: 44,347,251 (GRCm39)	Y290C	probably damaging	Het
Vmn1r216	A	T	13: 23,283,455 (GRCm39)	D46V	probably damaging	Het
Wdr17	A	T	8: 55,112,735 (GRCm39)	F789I	probably damaging	Het
Zfp266	A	G	9: 20,411,746 (GRCm39)	S144P	probably benign	Het

Other mutations in Or5k3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01937:Or5k3	APN	16	58,969,827 (GRCm39)	missense	probably benign	0.01
IGL01945:Or5k3	APN	16	58,969,827 (GRCm39)	missense	probably benign	0.01
R0071:Or5k3	UTSW	16	58,969,578 (GRCm39)	missense	probably benign	0.23
R0390:Or5k3	UTSW	16	58,969,662 (GRCm39)	missense	probably benign	0.01
R0601:Or5k3	UTSW	16	58,970,117 (GRCm39)	missense	probably benign	0.00
R1499:Or5k3	UTSW	16	58,969,287 (GRCm39)	missense	probably benign
R1612:Or5k3	UTSW	16	58,969,987 (GRCm39)	missense	probably benign	0.00
R1785:Or5k3	UTSW	16	58,969,660 (GRCm39)	missense	probably damaging	1.00
R2082:Or5k3	UTSW	16	58,969,248 (GRCm39)	missense	probably damaging	0.99
R3605:Or5k3	UTSW	16	58,969,846 (GRCm39)	missense	probably damaging	1.00
R4168:Or5k3	UTSW	16	58,969,363 (GRCm39)	missense	probably benign	0.00
R4839:Or5k3	UTSW	16	58,969,393 (GRCm39)	missense	probably damaging	1.00
R4989:Or5k3	UTSW	16	58,969,981 (GRCm39)	missense	probably damaging	1.00
R5285:Or5k3	UTSW	16	58,969,633 (GRCm39)	missense	probably damaging	1.00
R7107:Or5k3	UTSW	16	58,969,279 (GRCm39)	missense	probably benign	0.10
R7136:Or5k3	UTSW	16	58,969,327 (GRCm39)	missense	probably damaging	1.00
R7317:Or5k3	UTSW	16	58,969,684 (GRCm39)	missense	possibly damaging	0.80
R7601:Or5k3	UTSW	16	58,969,597 (GRCm39)	missense	probably benign	0.01
R7729:Or5k3	UTSW	16	58,969,570 (GRCm39)	missense	probably damaging	1.00
R8004:Or5k3	UTSW	16	58,969,351 (GRCm39)	missense	probably damaging	1.00
R8208:Or5k3	UTSW	16	58,969,382 (GRCm39)	missense	probably benign	0.41
R8282:Or5k3	UTSW	16	58,969,529 (GRCm39)	nonsense	probably null
R8954:Or5k3	UTSW	16	58,969,319 (GRCm39)	nonsense	probably null
R9137:Or5k3	UTSW	16	58,969,635 (GRCm39)	missense	probably benign	0.30
R9197:Or5k3	UTSW	16	58,969,489 (GRCm39)	missense	probably damaging	0.99
R9396:Or5k3	UTSW	16	58,969,302 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- AAGCCTTATCAACATGCGCTTC -3'
(R):5'- CGGTGACTAGATACATTCCATTACTTC -3'

Sequencing Primer
(F):5'- AACATGCGCTTCCCACTTTCTG -3'
(R):5'- TTCCTAAGAATTTCAAGCATG -3'

Posted On

2022-07-18