Mutagenetix > Incidental Mutation

Incidental Mutation 'R9553:Or5w14'

720590

Institutional Source

Beutler Lab

Gene Symbol

Or5w14

Ensembl Gene

ENSMUSG00000075150

Gene Name

olfactory receptor family 5 subfamily W member 14

Synonyms

Olfr1137, MOR177-20, GA_x6K02T2Q125-49215724-49214792, MOR40-9P

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R9553 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

87541316-87542248 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 87541992 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 86 (V86E)

Ref Sequence

ENSEMBL: ENSMUSP00000149311 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099850] [ENSMUST00000214209]

AlphaFold

Q7TR40

Predicted Effect

probably benign
Transcript: ENSMUST00000099850
AA Change: V86E

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000097437
Gene: ENSMUSG00000075150
AA Change: V86E

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-45	PFAM
Pfam:7tm_1	41	290	2.8e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214209
AA Change: V86E

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410002F23Rik	C	T	7: 43,900,334 (GRCm39)	R80W	probably damaging	Het
Ankdd1b	G	T	13: 96,591,294 (GRCm39)	N68K	possibly damaging	Het
Arhgef1	A	G	7: 24,619,115 (GRCm39)	E452G	probably damaging	Het
Cbx8	T	G	11: 118,930,964 (GRCm39)	E45D	probably damaging	Het
Ceacam15	T	C	7: 16,407,316 (GRCm39)	Y67C	probably damaging	Het
Ceacam20	A	G	7: 19,723,926 (GRCm39)	Y570C	probably damaging	Het
Cnga4	A	G	7: 105,054,977 (GRCm39)	Y187C	probably damaging	Het
Cntrob	T	C	11: 69,205,679 (GRCm39)	N385S	probably benign	Het
Coro7	A	G	16: 4,486,624 (GRCm39)	V183A	possibly damaging	Het
Cyp2t4	G	A	7: 26,854,717 (GRCm39)	V66M	possibly damaging	Het
Dnah7b	A	G	1: 46,264,956 (GRCm39)	Y2150C	probably damaging	Het
E2f8	A	G	7: 48,528,394 (GRCm39)	S25P	probably damaging	Het
Eif1ad7	A	G	12: 88,238,476 (GRCm39)	Y95H	probably damaging	Het
Eif3d	T	C	15: 77,843,837 (GRCm39)	E503G	probably damaging	Het
Elp6	T	A	9: 110,144,965 (GRCm39)	V157D	probably damaging	Het
Entpd3	T	C	9: 120,387,546 (GRCm39)	Y248H	probably damaging	Het
Fam186a	T	C	15: 99,844,561 (GRCm39)	E561G	unknown	Het
Fbxl13	C	T	5: 21,728,151 (GRCm39)	G519S	probably damaging	Het
Fgfr4	T	G	13: 55,309,228 (GRCm39)	S422A	probably damaging	Het
Flg2	G	A	3: 93,121,901 (GRCm39)	C1357Y	unknown	Het
Fpr3	T	A	17: 18,191,612 (GRCm39)	N294K	probably damaging	Het
Gas6	T	C	8: 13,525,048 (GRCm39)	Q312R	possibly damaging	Het
Glg1	C	T	8: 111,926,770 (GRCm39)	E182K	probably benign	Het
Gucy1b1	T	C	3: 81,947,087 (GRCm39)	D374G	probably damaging	Het
Ifrd2	C	T	9: 107,468,285 (GRCm39)	T251I	possibly damaging	Het
Igkv4-91	C	T	6: 68,745,632 (GRCm39)	G89R	possibly damaging	Het
Kcnk1	C	A	8: 126,756,322 (GRCm39)	Y281*	probably null	Het
Kcp	G	T	6: 29,485,100 (GRCm39)	F1217L	probably null	Het
Lama3	G	A	18: 12,563,019 (GRCm39)	G514D	probably damaging	Het
Madd	A	G	2: 91,008,800 (GRCm39)	L34P	probably damaging	Het
Mak	T	A	13: 41,183,595 (GRCm39)	T562S	probably benign	Het
Nfatc4	A	G	14: 56,070,259 (GRCm39)	E879G	probably damaging	Het
Pcdhb19	C	T	18: 37,631,848 (GRCm39)	R548C	probably damaging	Het
Peli2	A	G	14: 48,488,150 (GRCm39)	I165V	probably damaging	Het
Plekhd1	A	T	12: 80,753,977 (GRCm39)	M148L	probably benign	Het
Rars2	G	A	4: 34,637,014 (GRCm39)	G172R	probably damaging	Het
Reck	T	C	4: 43,928,310 (GRCm39)	V537A	probably damaging	Het
Rnf135	T	A	11: 80,074,758 (GRCm39)	S6T	probably benign	Het
Sirpb1a	C	T	3: 15,476,320 (GRCm39)	C226Y	probably damaging	Het
Speg	A	C	1: 75,394,645 (GRCm39)	I1785L	probably benign	Het
Spry4	T	C	18: 38,723,070 (GRCm39)	N231S	probably damaging	Het
Tchh	C	T	3: 93,355,125 (GRCm39)	Q1522*	probably null	Het
Tm7sf3	C	T	6: 146,525,179 (GRCm39)	D89N	possibly damaging	Het
Trav7d-3	G	T	14: 52,981,820 (GRCm39)		probably benign	Het
Vmn2r49	A	C	7: 9,720,849 (GRCm39)	V214G	probably benign	Het
Vmn2r80	T	C	10: 78,984,743 (GRCm39)	Y32H	probably benign	Het
Vwf	A	C	6: 125,577,662 (GRCm39)	D501A		Het
Zfp428	A	G	7: 24,214,866 (GRCm39)	T161A	possibly damaging	Het
Zpld2	G	A	4: 133,929,312 (GRCm39)	P331L	probably benign	Het

Other mutations in Or5w14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Or5w14	APN	2	87,541,477 (GRCm39)	missense	possibly damaging	0.76
IGL02225:Or5w14	APN	2	87,541,743 (GRCm39)	missense	possibly damaging	0.78
IGL02312:Or5w14	APN	2	87,541,353 (GRCm39)	missense	probably benign	0.00
IGL03326:Or5w14	APN	2	87,542,039 (GRCm39)	missense	probably damaging	1.00
R0432:Or5w14	UTSW	2	87,541,774 (GRCm39)	missense	probably benign	0.13
R1656:Or5w14	UTSW	2	87,541,422 (GRCm39)	missense	possibly damaging	0.94
R1852:Or5w14	UTSW	2	87,541,317 (GRCm39)	splice site	probably null
R1880:Or5w14	UTSW	2	87,541,639 (GRCm39)	missense	probably damaging	1.00
R2184:Or5w14	UTSW	2	87,541,549 (GRCm39)	missense	probably damaging	1.00
R2511:Or5w14	UTSW	2	87,541,392 (GRCm39)	missense	probably damaging	1.00
R4356:Or5w14	UTSW	2	87,542,229 (GRCm39)	missense	possibly damaging	0.95
R6184:Or5w14	UTSW	2	87,542,188 (GRCm39)	missense	probably benign
R6278:Or5w14	UTSW	2	87,541,815 (GRCm39)	nonsense	probably null
R6621:Or5w14	UTSW	2	87,541,899 (GRCm39)	missense	probably benign	0.10
R7549:Or5w14	UTSW	2	87,542,115 (GRCm39)	missense	probably damaging	1.00
R7799:Or5w14	UTSW	2	87,541,428 (GRCm39)	missense	possibly damaging	0.88
R8187:Or5w14	UTSW	2	87,541,624 (GRCm39)	missense	probably benign	0.14
R8236:Or5w14	UTSW	2	87,542,104 (GRCm39)	missense	possibly damaging	0.63
R8298:Or5w14	UTSW	2	87,541,376 (GRCm39)	missense	probably damaging	1.00
R8314:Or5w14	UTSW	2	87,541,546 (GRCm39)	missense	probably benign	0.00
R8398:Or5w14	UTSW	2	87,542,175 (GRCm39)	missense	probably benign
R9121:Or5w14	UTSW	2	87,541,975 (GRCm39)	missense	probably damaging	0.99
R9378:Or5w14	UTSW	2	87,541,423 (GRCm39)	missense	possibly damaging	0.88
R9732:Or5w14	UTSW	2	87,541,489 (GRCm39)	missense	possibly damaging	0.78

Predicted Primers

PCR Primer
(F):5'- ATGCCAGTGTTGTATGTATCAAACC -3'
(R):5'- TGCCAGAATTCTTTCTCTTGGG -3'

Sequencing Primer
(F):5'- TCAAACCATCTATAGTACTTGTCAGG -3'
(R):5'- CCAGAATTCTTTCTCTTGGGTATTAG -3'

Posted On

2022-08-09