Mutagenetix > Incidental Mutation

Incidental Mutation 'R9553:Fpr3'

720634

Institutional Source

Beutler Lab

Gene Symbol

Fpr3

Ensembl Gene

ENSMUSG00000079700

Gene Name

formyl peptide receptor 3

Synonyms

Fprl1, LXA4-R, Lxa4r, Fpr-rs1

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9553 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

18190720-18191939 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 18191612 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 294 (N294K)

Ref Sequence

ENSEMBL: ENSMUSP00000093316 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054871] [ENSMUST00000115565]

AlphaFold

O08790

Predicted Effect

probably damaging
Transcript: ENSMUST00000054871
AA Change: N294K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000093316
Gene: ENSMUSG00000079700
AA Change: N294K

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	302	2.2e-43	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000115565
AA Change: N294K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000111227
Gene: ENSMUSG00000079700
AA Change: N294K

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	302	1.5e-36	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410002F23Rik	C	T	7: 43,900,334 (GRCm39)	R80W	probably damaging	Het
Ankdd1b	G	T	13: 96,591,294 (GRCm39)	N68K	possibly damaging	Het
Arhgef1	A	G	7: 24,619,115 (GRCm39)	E452G	probably damaging	Het
Cbx8	T	G	11: 118,930,964 (GRCm39)	E45D	probably damaging	Het
Ceacam15	T	C	7: 16,407,316 (GRCm39)	Y67C	probably damaging	Het
Ceacam20	A	G	7: 19,723,926 (GRCm39)	Y570C	probably damaging	Het
Cnga4	A	G	7: 105,054,977 (GRCm39)	Y187C	probably damaging	Het
Cntrob	T	C	11: 69,205,679 (GRCm39)	N385S	probably benign	Het
Coro7	A	G	16: 4,486,624 (GRCm39)	V183A	possibly damaging	Het
Cyp2t4	G	A	7: 26,854,717 (GRCm39)	V66M	possibly damaging	Het
Dnah7b	A	G	1: 46,264,956 (GRCm39)	Y2150C	probably damaging	Het
E2f8	A	G	7: 48,528,394 (GRCm39)	S25P	probably damaging	Het
Eif1ad7	A	G	12: 88,238,476 (GRCm39)	Y95H	probably damaging	Het
Eif3d	T	C	15: 77,843,837 (GRCm39)	E503G	probably damaging	Het
Elp6	T	A	9: 110,144,965 (GRCm39)	V157D	probably damaging	Het
Entpd3	T	C	9: 120,387,546 (GRCm39)	Y248H	probably damaging	Het
Fam186a	T	C	15: 99,844,561 (GRCm39)	E561G	unknown	Het
Fbxl13	C	T	5: 21,728,151 (GRCm39)	G519S	probably damaging	Het
Fgfr4	T	G	13: 55,309,228 (GRCm39)	S422A	probably damaging	Het
Flg2	G	A	3: 93,121,901 (GRCm39)	C1357Y	unknown	Het
Gas6	T	C	8: 13,525,048 (GRCm39)	Q312R	possibly damaging	Het
Glg1	C	T	8: 111,926,770 (GRCm39)	E182K	probably benign	Het
Gucy1b1	T	C	3: 81,947,087 (GRCm39)	D374G	probably damaging	Het
Ifrd2	C	T	9: 107,468,285 (GRCm39)	T251I	possibly damaging	Het
Igkv4-91	C	T	6: 68,745,632 (GRCm39)	G89R	possibly damaging	Het
Kcnk1	C	A	8: 126,756,322 (GRCm39)	Y281*	probably null	Het
Kcp	G	T	6: 29,485,100 (GRCm39)	F1217L	probably null	Het
Lama3	G	A	18: 12,563,019 (GRCm39)	G514D	probably damaging	Het
Madd	A	G	2: 91,008,800 (GRCm39)	L34P	probably damaging	Het
Mak	T	A	13: 41,183,595 (GRCm39)	T562S	probably benign	Het
Nfatc4	A	G	14: 56,070,259 (GRCm39)	E879G	probably damaging	Het
Or5w14	A	T	2: 87,541,992 (GRCm39)	V86E	probably benign	Het
Pcdhb19	C	T	18: 37,631,848 (GRCm39)	R548C	probably damaging	Het
Peli2	A	G	14: 48,488,150 (GRCm39)	I165V	probably damaging	Het
Plekhd1	A	T	12: 80,753,977 (GRCm39)	M148L	probably benign	Het
Rars2	G	A	4: 34,637,014 (GRCm39)	G172R	probably damaging	Het
Reck	T	C	4: 43,928,310 (GRCm39)	V537A	probably damaging	Het
Rnf135	T	A	11: 80,074,758 (GRCm39)	S6T	probably benign	Het
Sirpb1a	C	T	3: 15,476,320 (GRCm39)	C226Y	probably damaging	Het
Speg	A	C	1: 75,394,645 (GRCm39)	I1785L	probably benign	Het
Spry4	T	C	18: 38,723,070 (GRCm39)	N231S	probably damaging	Het
Tchh	C	T	3: 93,355,125 (GRCm39)	Q1522*	probably null	Het
Tm7sf3	C	T	6: 146,525,179 (GRCm39)	D89N	possibly damaging	Het
Trav7d-3	G	T	14: 52,981,820 (GRCm39)		probably benign	Het
Vmn2r49	A	C	7: 9,720,849 (GRCm39)	V214G	probably benign	Het
Vmn2r80	T	C	10: 78,984,743 (GRCm39)	Y32H	probably benign	Het
Vwf	A	C	6: 125,577,662 (GRCm39)	D501A		Het
Zfp428	A	G	7: 24,214,866 (GRCm39)	T161A	possibly damaging	Het
Zpld2	G	A	4: 133,929,312 (GRCm39)	P331L	probably benign	Het

Other mutations in Fpr3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Fpr3	APN	17	18,190,828 (GRCm39)	missense	probably benign	0.17
IGL01520:Fpr3	APN	17	18,191,325 (GRCm39)	missense	possibly damaging	0.69
IGL02166:Fpr3	APN	17	18,190,726 (GRCm39)	utr 5 prime	probably benign
IGL02380:Fpr3	APN	17	18,191,254 (GRCm39)	missense	probably benign	0.00
IGL02587:Fpr3	APN	17	18,190,953 (GRCm39)	missense	probably benign	0.12
R1521:Fpr3	UTSW	17	18,191,277 (GRCm39)	missense	probably damaging	1.00
R1533:Fpr3	UTSW	17	18,190,922 (GRCm39)	nonsense	probably null
R1913:Fpr3	UTSW	17	18,191,670 (GRCm39)	missense	probably damaging	0.96
R2099:Fpr3	UTSW	17	18,191,443 (GRCm39)	missense	probably damaging	1.00
R2140:Fpr3	UTSW	17	18,190,879 (GRCm39)	missense	probably damaging	1.00
R2206:Fpr3	UTSW	17	18,190,908 (GRCm39)	missense	probably damaging	1.00
R2219:Fpr3	UTSW	17	18,191,644 (GRCm39)	missense	possibly damaging	0.93
R2224:Fpr3	UTSW	17	18,191,455 (GRCm39)	missense	probably damaging	1.00
R2244:Fpr3	UTSW	17	18,191,449 (GRCm39)	missense	probably benign	0.03
R2994:Fpr3	UTSW	17	18,191,130 (GRCm39)	nonsense	probably null
R5364:Fpr3	UTSW	17	18,190,806 (GRCm39)	missense	probably benign	0.00
R6179:Fpr3	UTSW	17	18,190,919 (GRCm39)	nonsense	probably null
R6781:Fpr3	UTSW	17	18,190,978 (GRCm39)	missense	probably benign	0.09
R6909:Fpr3	UTSW	17	18,191,429 (GRCm39)	missense	probably benign	0.00
R7565:Fpr3	UTSW	17	18,191,227 (GRCm39)	missense	probably damaging	1.00
R8008:Fpr3	UTSW	17	18,191,715 (GRCm39)	missense	probably benign	0.03
R8097:Fpr3	UTSW	17	18,191,054 (GRCm39)	missense	probably damaging	1.00
R8351:Fpr3	UTSW	17	18,191,436 (GRCm39)	missense	probably benign	0.27
R8732:Fpr3	UTSW	17	18,191,223 (GRCm39)	missense	possibly damaging	0.94
R8994:Fpr3	UTSW	17	18,191,341 (GRCm39)	missense	possibly damaging	0.92
R9076:Fpr3	UTSW	17	18,191,725 (GRCm39)	missense	probably benign
R9206:Fpr3	UTSW	17	18,191,131 (GRCm39)	missense	probably damaging	1.00
R9208:Fpr3	UTSW	17	18,191,131 (GRCm39)	missense	probably damaging	1.00
R9327:Fpr3	UTSW	17	18,191,713 (GRCm39)	missense	probably damaging	1.00
X0021:Fpr3	UTSW	17	18,191,500 (GRCm39)	missense	probably benign	0.06
Z1176:Fpr3	UTSW	17	18,191,255 (GRCm39)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- AAGCCTTTGTTAATTCCAGCCG -3'
(R):5'- TAAGGTGGGACAAAACACATCC -3'

Sequencing Primer
(F):5'- GTCCTTTCCGAGTTCTTACAGGAG -3'
(R):5'- ACAGCCCCCTCCTCATATTG -3'

Posted On

2022-08-09