Incidental Mutation 'R9555:Zfp84'
| ID |
720710 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp84
|
| Ensembl Gene |
ENSMUSG00000046185 |
| Gene Name |
zinc finger protein 84 |
| Synonyms |
KRAB18, C86188, Zfp69, 2210410P13Rik, 4633401C23Rik |
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R9555 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
29467977-29479246 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to T
at 29476102 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Phenylalanine
at position 265
(V265F)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000032802
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000032802]
|
| AlphaFold |
Q9D654 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000032802
AA Change: V265F
PolyPhen 2
Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000032802
Gene: ENSMUSG00000046185
AA Change: V265F
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
14 |
74 |
9.09e-36 |
SMART |
|
ZnF_C2H2
|
249 |
271 |
1.67e-2 |
SMART |
|
ZnF_C2H2
|
277 |
299 |
1.43e-1 |
SMART |
|
ZnF_C2H2
|
305 |
327 |
5.81e-2 |
SMART |
|
ZnF_C2H2
|
333 |
355 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
361 |
383 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
389 |
411 |
2.32e-1 |
SMART |
|
ZnF_C2H2
|
417 |
439 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
445 |
467 |
1.69e-3 |
SMART |
|
ZnF_C2H2
|
473 |
495 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
501 |
523 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
529 |
551 |
1.58e-3 |
SMART |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.5%
- 20x: 98.6%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 38 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930453N24Rik |
A |
G |
16: 64,586,947 (GRCm39) |
V259A |
probably benign |
Het |
| Abcd4 |
T |
C |
12: 84,661,949 (GRCm39) |
I63V |
probably benign |
Het |
| Agps |
C |
A |
2: 75,683,091 (GRCm39) |
P139T |
probably damaging |
Het |
| B130006D01Rik |
A |
T |
11: 95,616,956 (GRCm39) |
H27L |
unknown |
Het |
| BC024139 |
A |
G |
15: 76,005,359 (GRCm39) |
V501A |
possibly damaging |
Het |
| Cacul1 |
A |
T |
19: 60,533,887 (GRCm39) |
Y238* |
probably null |
Het |
| Cep55 |
T |
C |
19: 38,059,592 (GRCm39) |
|
probably null |
Het |
| Csf1r |
T |
A |
18: 61,243,473 (GRCm39) |
I163N |
possibly damaging |
Het |
| Ddx3y |
G |
T |
Y: 1,265,895 (GRCm39) |
D367E |
probably benign |
Het |
| Ecpas |
T |
A |
4: 58,879,083 (GRCm39) |
I69L |
possibly damaging |
Het |
| Epb41l4a |
A |
G |
18: 34,009,966 (GRCm39) |
V213A |
possibly damaging |
Het |
| Idh2 |
TCCCAGG |
T |
7: 79,748,079 (GRCm39) |
|
probably benign |
Het |
| Klrb1a |
T |
C |
6: 128,595,427 (GRCm39) |
E142G |
probably damaging |
Het |
| Lamb3 |
A |
T |
1: 193,011,113 (GRCm39) |
I369F |
possibly damaging |
Het |
| Lig1 |
T |
C |
7: 13,025,400 (GRCm39) |
V254A |
probably benign |
Het |
| Lrp1b |
T |
A |
2: 40,741,693 (GRCm39) |
D3192V |
|
Het |
| Lrp1b |
T |
C |
2: 40,748,438 (GRCm39) |
D3134G |
|
Het |
| Map1s |
T |
A |
8: 71,367,236 (GRCm39) |
S714T |
probably benign |
Het |
| Marchf2 |
A |
G |
17: 33,922,129 (GRCm39) |
L77P |
probably damaging |
Het |
| Nek8 |
A |
G |
11: 78,067,390 (GRCm39) |
F15L |
probably benign |
Het |
| Or1s2 |
T |
A |
19: 13,758,360 (GRCm39) |
I126N |
probably damaging |
Het |
| Pdzrn4 |
C |
T |
15: 92,297,703 (GRCm39) |
R223C |
probably damaging |
Het |
| Per1 |
T |
A |
11: 68,995,574 (GRCm39) |
V718D |
probably benign |
Het |
| Pik3ca |
A |
G |
3: 32,505,916 (GRCm39) |
H676R |
probably damaging |
Het |
| Pla2g4e |
C |
A |
2: 120,075,400 (GRCm39) |
|
probably benign |
Het |
| Proser1 |
A |
T |
3: 53,378,876 (GRCm39) |
I103F |
possibly damaging |
Het |
| Pyroxd1 |
G |
A |
6: 142,300,421 (GRCm39) |
A184T |
possibly damaging |
Het |
| Rin2 |
C |
T |
2: 145,718,415 (GRCm39) |
Q632* |
probably null |
Het |
| Serpinb12 |
C |
T |
1: 106,884,345 (GRCm39) |
H364Y |
probably damaging |
Het |
| Sfxn3 |
G |
A |
19: 45,038,211 (GRCm39) |
E64K |
probably benign |
Het |
| Slc38a1 |
C |
T |
15: 96,486,860 (GRCm39) |
V199M |
possibly damaging |
Het |
| Tep1 |
A |
G |
14: 51,105,888 (GRCm39) |
I44T |
possibly damaging |
Het |
| Trip13 |
T |
A |
13: 74,084,252 (GRCm39) |
T78S |
probably benign |
Het |
| Trmt11 |
T |
C |
10: 30,470,150 (GRCm39) |
D58G |
possibly damaging |
Het |
| Usp28 |
A |
T |
9: 48,952,736 (GRCm39) |
I1015F |
probably damaging |
Het |
| Vcan |
T |
C |
13: 89,839,659 (GRCm39) |
T1962A |
|
Het |
| Vmn2r100 |
T |
A |
17: 19,743,857 (GRCm39) |
S507T |
probably benign |
Het |
| Vmn2r91 |
C |
T |
17: 18,325,792 (GRCm39) |
P137S |
possibly damaging |
Het |
|
| Other mutations in Zfp84 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01768:Zfp84
|
APN |
7 |
29,476,091 (GRCm39) |
missense |
probably benign |
0.16 |
|
IGL03022:Zfp84
|
APN |
7 |
29,474,759 (GRCm39) |
splice site |
probably benign |
|
|
R0666:Zfp84
|
UTSW |
7 |
29,476,276 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0781:Zfp84
|
UTSW |
7 |
29,470,797 (GRCm39) |
start codon destroyed |
probably null |
0.02 |
|
R1110:Zfp84
|
UTSW |
7 |
29,470,797 (GRCm39) |
start codon destroyed |
probably null |
0.02 |
|
R1353:Zfp84
|
UTSW |
7 |
29,475,600 (GRCm39) |
missense |
probably benign |
0.02 |
|
R1495:Zfp84
|
UTSW |
7 |
29,476,728 (GRCm39) |
nonsense |
probably null |
|
|
R1496:Zfp84
|
UTSW |
7 |
29,476,039 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R1681:Zfp84
|
UTSW |
7 |
29,476,825 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1827:Zfp84
|
UTSW |
7 |
29,476,768 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R1854:Zfp84
|
UTSW |
7 |
29,474,796 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R2209:Zfp84
|
UTSW |
7 |
29,476,607 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R2843:Zfp84
|
UTSW |
7 |
29,474,758 (GRCm39) |
splice site |
probably null |
|
|
R2844:Zfp84
|
UTSW |
7 |
29,474,758 (GRCm39) |
splice site |
probably null |
|
|
R4691:Zfp84
|
UTSW |
7 |
29,476,505 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5453:Zfp84
|
UTSW |
7 |
29,475,722 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R5474:Zfp84
|
UTSW |
7 |
29,476,514 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5578:Zfp84
|
UTSW |
7 |
29,474,856 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R5646:Zfp84
|
UTSW |
7 |
29,475,818 (GRCm39) |
missense |
probably benign |
0.05 |
|
R5963:Zfp84
|
UTSW |
7 |
29,476,378 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6830:Zfp84
|
UTSW |
7 |
29,475,911 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8129:Zfp84
|
UTSW |
7 |
29,475,862 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8138:Zfp84
|
UTSW |
7 |
29,474,797 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9180:Zfp84
|
UTSW |
7 |
29,474,873 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R9401:Zfp84
|
UTSW |
7 |
29,476,297 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9489:Zfp84
|
UTSW |
7 |
29,476,264 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R9605:Zfp84
|
UTSW |
7 |
29,476,264 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
V3553:Zfp84
|
UTSW |
7 |
29,476,672 (GRCm39) |
missense |
probably benign |
0.36 |
|
Z1186:Zfp84
|
UTSW |
7 |
29,470,805 (GRCm39) |
missense |
probably damaging |
0.98 |
|
| Predicted Primers |
PCR Primer
(F):5'- CACAGGTGAGATGAAGCGTC -3'
(R):5'- AGGCCTTCTGACATTTGTGGC -3'
Sequencing Primer
(F):5'- GCGTCAGCAGGACTTTGC -3'
(R):5'- CTTCTGACATTTGTGGCATTCG -3'
|
| Posted On |
2022-08-09 |
Incidental Mutation